HPLC法同时测定半枝莲中黄酮和生物碱类成分

Simultaneous Determination of Flavones and Scutebarbatines in Scutellariae barbatae by HPLC

    摘要
  • 摘要: 目的 建立同时测定半枝莲饮片中木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和半枝莲生物碱B的HPLC法。方法 采用高效液相色谱法，色谱柱为Phenomenon C18柱(250 mm×4.60 mm，5 μm)，以乙腈-水溶液为流动相，梯度洗脱；流速为1.0 mL/min，柱温为30 ℃；木犀草素、芹菜素检测波长为355 nm，汉黄芩素、半枝莲生物碱A和生物碱B检测波长为264 nm。结果 木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和生物碱B分别在0.0374~2.40 μg、0.0312~2.00 μg、0.0312~2.00 μg、0.0062~0.40 μg和0.0062~0.40 μg内线性关系良好。精密度，重复性及加样回收均符合要求。结论 此方法快速简便，重现性好，可用于半枝莲饮片的质量控制。

     

    Abstract: OBJECTIVE To establish HPLC methods for simultaneous determination of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B.METHODS The analytical column was Phenomenex Luna C18(250 mm×4.60 mm， 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The flow rate was 1.0 mL/min; The column temperature was 30 ℃. Luteolin and apigenin were detected at 355 nm; wogonin， scutebarbatine A and scutebarbatine B were detected at 264 nm.RESULTS The ranges of calibration curve of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B were 0.037 4~2.40 μg、0.031 2~2.00 μg、0.031 2~2.00 μg、0.006 2~0.40 μg and 0.006 2~0.40 μg， respectively. Also， the intra-batch and inter-batch variations and the average recoveries were all meet the requirement of Chinese Pharmacopoeia.CONCLUSION The method is rapid and simple with a good reproducibility and it can be used for the quality control of herba Scutellariae barbatae.

     

    • HTML全文
    • 参考文献(6)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回