清肺平喘胶囊治疗哮喘的作用机制研究
Research on Mechanism of Qingfei Pingchuan Capsule Treating Asthma
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摘要: 目的 通过清肺平喘胶囊对哮喘大鼠IL-4、IL-13等细胞因子，炎症介质IgE，脂质过氧化物MDA的影响以探讨清肺平喘胶囊治疗哮喘的作用机制。方法 制作哮喘大鼠模型，分别测定各组大鼠BALF和血清中IL-4、IL-13、IgE、MDA含量。结果 与生理盐水组比较，模型组大鼠BALF以及血清中IL-4、IL-13、IgE、MDA含量均显著升高（P<0.01，P<0.05），模型大鼠BALF中IL-4含量与MDA、IgE含量呈显著正相关(r=0.83，0.96，P<0.05)；与模型组比较，给药组大鼠BALF与血清中IL-4、IL-13、IgE、MDA含量均显著降低（P<0.01，P<0.05），模型大鼠血清中与IL-4含量与MDA、IgE含量呈显著正相关(r=0.86，0.88，P<0.05)。结论 清肺平喘胶囊能通过降低脂质过氧化物MDA、IL-4、IL-13相关细胞因子的表达，减少炎性介质IgE的增加，有效地阻止哮喘炎症的发生，减轻气道炎症及缓解气道高反应性发展，避免哮喘进一步加重。Abstract: OBJECTIVE To explore the mechanism of Qingfei Pingchuan Capsule treating on asthma by discussing its function on asthma rats' IL-4， IL-13 cytokines， IgE inflammatory mediator， lipid peroxide MDA. METHODS The asthma rats' model were established by allergen， then the rats' BALF and IL-4， IL-13， IgE， MDA in serum were tested respectively.RESULTS Compared with saline group， the model group rats' BALF and IL-4， IL-13， IgE， MDA in serum were significantly higher(P<0.01， P<0.05)， and the contents of IL-4 in BALF in the model group have the positive correlation with the contents of MDA and IgE(r=0.83， 0.96， P<0.05). Compared with the model group， treatment group rats' BALF and IL-4， IL-13， IgE， MDA in serum were significantly lower(P<0.01， P<0.05)， and the contents of IL-4 in BALF in the model group have the positive correlation with the contents of MDA and IgE(r=0.86， 0.88， P<0.05).CONCLUSION Qingfei Pingchuan capsule can bring down the expression of MDA， IL-4， IL-13 cytokines， reduce the increasing inflammatory IgE， prevent asthma inflammation， relieve airway inflammation and ease its high-response， and avoid the aggravation of asthma.
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