Abstract: OBJECTIVE To observe the effect of ginger moxibustion on vasoactive intestinal peptide(VIP) and interleukin-10(IL-10) in gastric cancer rats.METHODS The gastric carcinoma models， established by N-methyl-N-nitro-N-nitrosoguanidine(MNNG)， were randomly divided into 4 groups: VIP group(n=10， 10 μg/100 mL)，VIP antagonist group(n=10， 10 μg/100 mL)， model group(n=10， the normal breeding) and ginger moxibustion group(ginger moxibustion， n=10， on zhongwan， pishu， weishu， zusanli， neiguan， etc). Apart from that， blank group(n=10， physiological saline) was set up additionally. Three weeks later， the contents of VIP， IL-10 were tested， and the expression of proteins VIP and mRNA in gastric cancer tissues were detected by immunohistrochemistry and RT-PCR methods.RESULTS VIP and IL-10 in model group and were reduced， and the ones in ginger moxibustion group were increased obviously(P<0.01)， which have significant differences with the VIP group. Gastric carcinoma group have VIP-positive immunoreactive cells， which were not found in normal gastric tissues. There was VIP mRNA expression in each groups' gastric cancer tissue. The expression of mRNA in Ginger moxibustion and VIP groups were reduced， but ginger moxibustion group was more significantly reduced(P<0.01).CONCLUSION Gastric cancer rats can be treated with ginger moxibustion by inhibiting the detection of VIP， IL-10 and the expression of VIP mRNA.