Abstract: OBJECTIVE The previous study had shown that Bushen Yisui Capsule played an important role in the development and prevention of experimental autoimmune encephalomyelitis (EAE). In present study， the effects of Bushen Yisui Capsule on the expression of brain-derived neurotrophic factor (BDNF) and Nogo A in mice with EAE were discussed.METHODS The C57BL/6 mice were induced by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55. The body weights and neurological function scores of mice were examined everyday. The pathological changes in the brains of mice were observed with hematoxylin-eosin (H&E) staining， while the protein expression of BDNF and Nogo A were measured by immunohistochemistry (IHC) on Day 18 and Day 40 post-immunization (PI).RESULTS The data showed that inflammatory cells were reduced in the treatment group. The expression of BDNF obviously increased in the cerebral cortex (P<0.01)， the inferior to third ventricle (P<0.01) and the around of lateral ventricle (P<0.01) on Day 18 and Day 40 PI. In addition， the expression of Nogo A obviously decreased in the different areas of brain above (P<0.05， P<0.01).CONCLUSION Bushen Yisui Capsule has neuroprotective function in the mice with EAE， and the mechanism may be related with increasing expression of BDNF and decreasing expression of Nogo A.