Abstract: OBJECTIVE To analyze the content changes of heterophyllin A (HA) and B (HB) in Radix Pseudostellariae from different habitats， regions and harvest time， and to provide the basic information for exploring the quality forming process of Radix Pseudostellariae.METHODS The content of HA and HB was determined by RP-HPLC.RESULTS The contents of HA and HB in Radix Pseudostellariae from different habitats， regions and harvest time were variable. Dynamic change of HA and HB in Radix Pseudostellariae in different harvest time showed a regular pattern.CONCLUSION There are correlative differences between the content and the ratio of HA and HB in Radix Pseudostellariae from different habitats. The total amount of HA and HB is the highest in the traditional harvest time.