金欣口服液不同化学部位对呼吸道合胞病毒感染的影响
Experimental Study on Jinxin Oral Liquid Different Chemical Components Oppressing Respiratory Syncytial Virus
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摘要: 目的 观察金欣口服液不同化学部位在体内的抗呼吸道合胞病毒（RSV）作用。方法 小鼠经滴鼻感染RSV建立肺炎模型，随机分为正常对照组、模型对照组、利巴韦林组、金欣口服液组、金欣口服液石油醚、乙酸乙酯、正丁醇提取液组及母液组，光镜下观察肺组织的炎症病变情况，计算小鼠肺指数和肺指数抑制率、脾指数、胸腺指数、死亡保护率。结果 金欣口服液石油醚、乙酸乙酯、正丁醇提取液对RSV感染后小鼠的肺指数增高均有显著的降低作用(P<0.01)，减轻感染小鼠肺组织炎症病变，同时能增加脾指数和胸腺指数(P<0.01)。金欣口服液正丁醇提取液能降低RSV感染小鼠的死亡率。结论 金欣口服液石油醚、乙酸乙酯、正丁醇提取液对RSV具有一定的抑制作用，并且能提高RSV感染小鼠的免疫功能。正丁醇提取液对RSV感染小鼠具有一定的死亡保护作用。Abstract: OBJECTIVE To observe the effect of Jinxin Oral Liquid (JOL) different chemical components on respiratory syncytial virus (RSV).METHODS The mice pneumonia models were set up through nasal drops with RSV， which were randomly divided into normal control group， model control group， ribavirin group， JOL group， petroleum ether extract group， ethyl acetate extract group， N-butanol extract group and mother liquor group. The condition of the inflammatory lesion in lung tissues was observed under light microscope. Pulmonary index and pulmonary index inhibition rate， spleen index， thymus index and death protection rate were calculated.RESULTS Petroleum ether extract， ethyl acetate extract and n-butanol extract of JOL remarkably decreased pulmonary index of mice infected with RSV (P<0.01) and reduced the inflammatory lesions in lung tissues; meanwhile， increased spleen index and thymus index (P<0.01). N-butanol extract of JOL reduced the death rate of mice infected with RSV. CONCLUSION Petroleum ether extract， ethyl acetate extract and n-butanol extract of JOL have a certain inhibitory effect on RSV， and can improve the immune function of mice infected with RSV. N-butanol extract of JOL can make certain death protection for mice with RSV.
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