Abstract: OBJECTIVE To explore the effect of different fat and cholesterol feedstuff on the ApoE^-/- mice atherosclerosis.METHODS Eight 6-week old mice were randomly put into two even groups. The High fat and cholesterol group (H group) were given 21% fat， 1.25% cholesterol feedstuff for 14 weeks， and the Middle fat and cholesterol group (M group) were given 10% fat for 14 weeks. Then observe the changes of blood biochemistry， Lee's index， and body fat percentage and Artery pathological section.RESULTS The results shows H group and M group have not much difference on the blood biochemistry， Lee's index， body fat percentage and Artery pathological section. Both the routine HE dyeing section， oil red O staining show the H group and M group were in atherosclerosis fatty streak period， and a lot of macrophage macrophage foam cells were expressed in the plague， a large amount of necrotic corn and cholesterol crystals were existed. H group had arterial aneurysm in the aortic sinus， and M group had arterial aneurysm in the aorta abdominalis， and the cholesterol crystals in the H group are much more than the M group.CONCLUSION Both the two groups of different fat and cholesterol feedstuff have effect on the ApoE^-/- mice atherosclerosis， but the influence is not that much.