Volume 36 Issue 4
Jul.  2020
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2020  >  36(4): 509-516
CHENYong-gen, SANGMing, LUWu-guang, WANGMing-yuan, LIUJin-man, CHENSheng-jun, LISong, HUChun-ping, WANGQi, CAOPeng. Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(4): 509-516.
Citation: CHENYong-gen, SANGMing, LUWu-guang, WANGMing-yuan, LIUJin-man, CHENSheng-jun, LISong, HUChun-ping, WANGQi, CAOPeng. Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(4): 509-516.
shu

Development of a BmK Scorpion Quality Control Technology Based on Toxin Peptide BmK NaTx-13

  • 1.

    Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

  • 2.

    Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

  • 3.

    Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210028, China

  • 4.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 5.

    Jiangsu Key Laboratory of Key Technology for Preparation and Quality Control of Traditional Chinese Medicine Formula Particles, Jiangyin Tianjiang Pharmaceutical Co., Ltd, Wuxi, 214400, China

  • Publish Date: 2020-07-10
    Abstract
  • OBJECTIVE To develop a BmK Scorpion quality control technology based on toxin peptide. METHODS In this study， a novel sodium channel toxin BmK NaTx-13 was isolated from BV， and the specific polyclonal antibody against BmK NaTx-13 was obtained by immunizing rabbits. RESULTS A competitive enzyme linked immunosorbent assay (ELISA) based on BmK NaTx-13 specific antibody could detect the content of BmK NaTx-13 in the processed BmK scorpion extract and showed a good linear concentration dependent. CONCLUSION This research provided a rapid immunoassay method for establishing the quality detection processed BmK scorpion， and provided an insight for improving the quality standard of whole scorpion medicine.

     

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