Bacteriostasis of Prenylated Flavonoids from <i>Sophora Flavescens<\i> and Flavonoids from <i>Glycyrrhiza Uralensis<\i> Alone and Their Combination on <i>Staphylococcus Aureus<\i> and Experimental Mastitis

CAOYa-qi; WEIDan-dan; ZHANGSen; ZENGFei; GUOSheng; GUOJian-ming; DUANJin-ao

Volume 36 Issue 3

May 2020

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Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2020 > 36(3): 331-338

CAOYa-qi, WEIDan-dan, ZHANGSen, ZENGFei, GUOSheng, GUOJian-ming, DUANJin-ao. Bacteriostasis of Prenylated Flavonoids from Sophora Flavescens and Flavonoids from Glycyrrhiza Uralensis Alone and Their Combination on Staphylococcus Aureus and Experimental Mastitis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 331-338.

Citation:

CAOYa-qi, WEIDan-dan, ZHANGSen, ZENGFei, GUOSheng, GUOJian-ming, DUANJin-ao. Bacteriostasis of Prenylated Flavonoids from Sophora Flavescens<\i> and Flavonoids from Glycyrrhiza Uralensis<\i> Alone and Their Combination on Staphylococcus Aureus<\i> and Experimental Mastitis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 331-338.

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Bacteriostasis of Prenylated Flavonoids from Sophora Flavescens<\i> and Flavonoids from Glycyrrhiza Uralensis<\i> Alone and Their Combination on Staphylococcus Aureus<\i> and Experimental Mastitis

CAOYa-qi^1,2,

WEIDan-dan²,

ZHANGSen²,

ZENGFei²,

GUOSheng²,

GUOJian-ming²,

DUANJin-ao^1,2

1.
College of Pharmacy, Jiangsu University, Zhenjiang, 212013, China

2.
National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, 210023, China

Publish Date: 2020-05-10

Abstract

Abstract

OBJECTIVE To explore the antibacterial activity of prenylated flavonoids from Sophora flavecens and licorice flavonoids and their combination in vitro and in vivo， so as to provide scientific basis for the resource value discovery and comprehensive development of Sophora flavescens Ait. and Glycyrrhiza uralensis Fisch. METHODS The UPLC-Q-TOF-MS technology was applied to qualitatively analyze the prepared prenylated flavonoids from Sophora flavescens Ait. (KSHT) and licorice flavonoids (GCHT). The in vitro antibacterial activity was evaluated by investigating the effect of a single use and combined use of KSHT and GCHT on inhibiting the growth of Staphylococcus aureus and the generation of biofilm and flavin. The in vivo antibacterial activity was evaluated by building a mouse mastitis model induced by Staphylococcus aureus. RESULTS A total of 20 and 22 flavonoids were identified from KSHT and GCHT， respectively. KSHT outperformed GCHT in inhibiting the growth of Staphylococcus aureus and the generation of biofilm and flavin. Moreover， the combined use of KSHT and GCHT had better inhibitory effect than their independent use， suggesting that they have synergistic antibacterial action. Whether they were independently or jointly used in high or low doses， all the indexes of mastitis in mice were improved. Compared with the independent use of KSHT and GCHT， the combined use can better improve the viscera index and fungal burden of tissue in mice with mastitis. Regarding the improvement of indexes， KSHT was better than GCHT. In terms of the effect on improving the histopathology of mammary gland and reducing IL-1β， IL-2 and TNF-α in model group， GCHT was better than KSHT， but equivalent to the combination group. CONCLUSION The results of in vitro and in vivo tests indicated that the combined use of KSHT and GCHT has the best effect in resisting Staphylococcus aureus. It is expected that they can be jointly used in the prevention and treatment of mastitis in animal husbandry.

Keywords:
Sophora flavescens <\i>Ait.,

Glycyrrhiza uralensis<\i> Fisch.,

prenylated flavonoids,

Staphylococcus aureus<\i>,

bacteriostasis,

mastitis

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References(28)

References

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DING PL，HOU AJ，CHEN DF．Three new isoprenylated flavonoids from the roots of Sophora flavescens[J]．J Asian Nat Prod Res，2005，7(3)：237-243．

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SUN MY，CAO HY，SUN L，et al．Antitumor activities of Kushen： Literature review[J]．Evid Based Complement Alternat Med，2012，2012：373219．

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房康，陈辰，高荣凯，等．苦参总黄酮超声提取工艺及抑菌研究[J]．山东中医杂志，2016，35(11)：989-991．

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张福欣，宋佳烜，刘晓东，等．甘草黄酮抗氧化及免疫活性[J]．中国兽医学报，2019，39(6)：1180-1183．

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樊利军，张梦泽，韦艺媛，等．奶牛金黄色葡萄球菌乳腺炎小鼠模型的建立[J]．实验动物科学，2011，28(6)：1-6．

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SADER HS，FLAMM RK，JONES RN．Antimicrobial activity of daptomycin tested against Gram-positive pathogens collected in Europe，Latin America，and selected countries in the Asia-Pacific Region (2011)[J]．Diagn Microbiol Infect Dis，2013，75(4)：417-422．

[11]
谢强，林玉桓，苗淑萍，等．香芹酚对大肠杆菌和金黄色葡萄球菌细胞膜的影响[J]．食品工业科技，2014，35(23)：54-58，62．

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李晗．柠檬酸循环调节金黄色葡萄球菌金黄色素的产生[D]．上海：第二军医大学，2012．

[13]
ORHAN G，BAYRAM A，ZER Y，et al．Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis[J]．J Clin Microbiol，2005，43(1)：140-143．

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WENG ZB，ZENG F，ZHU ZH，et al. Comparative analysis of sixteen flavonoids from different parts of Sophora flavescens<\i> Ait. by ultra high-performance liquid chromatography-tandem mass spectrometry[J]. J Pharmaceut Biomed Anal，2018，156：214-220.

[15]
ZHANG L，XU L，XIAO SS，et al．Characterization of flavonoids in the extract of Sophora flavescens Ait．by high-performance liquid chromatography coupled with diode-array detector and electrospray ionization mass spectrometry[J]．J Pharm Biomed Anal，2007，44(5)：1019-1028．

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MA XC，XIN XL，ZHANG BJ，et al．Structural determination of flavonoids from Sophora flavescens[J]．Magn Reson Chem，2008，46(9)：903-906．

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MA XC，XIN XL，LIU KX，et al．Simultaneous determination of nine major flavonoids in Sophora flavescens by RP-LC[J]．Chromatographia，2008，68：471-474．

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赵艳敏，刘素香，张晨曦，等．基于HPLC-Q-TOF-MS技术的甘草化学成分分析[J]．中草药，2016，47(12)：2061-2068．

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马海娟，高简，张亚丽，等．基于HPLC-MSⁿ的甘草成分快速鉴定及质谱裂解途径研究[J]．中华中医药杂志，2018，33(3)：1120-1123．

[21]
赵鹏宇，焦利卫，张志明，等．UPLC-QTOF-MS研究不同国家甘草渣黄酮成分差异[J]．中国食品添加剂，2017(3)：111-116．

[22]
段天璇，马长华，王文全，等．HPLC-MS法鉴定甘草的指纹图谱[J]．中国药师，2009，12(4)：414-417．

[23]
张鲁，康雪芳，李红丽，等．甘草地上部分黄酮类成分HPLC-MS分析[J]．辽宁中医药大学学报，2018，20(5)：48-51．

[24]
LE MARECHAL C，THIERY R，VAUTOR E，et al．Mastitis impact on technological properties of milk and quality of milk products：a review[J]．Dairy Sci Technol，2011，91(3)：247-282．

[25]
许英民．抗生素替代物的种类及其在养禽上应用[J]．养禽与禽病防治，2016(3)：9-11．

[26]
丁丽军，袁华根．益生菌的作用及安全性研究进展[J]．兽医导刊，2018(2)：252．

[27]
何常明. 苦参和山豆根黄酮类成分及其生物活性的比较研究[D].上海：复旦大学，2010.

[28]
TSUKIYAMA R， KATSURA H， TOKURIKI N， et al. Antibacterial activity of licochalcone A against spore-forming bacteria[J]. Antimicrob Agents Chemother， 2002，46(5)：1226-1230.

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[1]	DING PL，HOU AJ，CHEN DF．Three new isoprenylated flavonoids from the roots of Sophora flavescens[J]．J Asian Nat Prod Res，2005，7(3)：237-243．
[2]	SUN MY，CAO HY，SUN L，et al．Antitumor activities of Kushen： Literature review[J]．Evid Based Complement Alternat Med，2012，2012：373219．
[3]	房康，陈辰，高荣凯，等．苦参总黄酮超声提取工艺及抑菌研究[J]．山东中医杂志，2016，35(11)：989-991．
[4]	张福欣，宋佳烜，刘晓东，等．甘草黄酮抗氧化及免疫活性[J]．中国兽医学报，2019，39(6)：1180-1183．
[5]	FUKAI T，MARUMO A，KAITOU K，et al．Antimicrobial activity of licorice flavonoids against methicillin-resistant Staphylococcus aureus[J]．Fitoterapia，2002，73(6)：536-539．
[6]	SEARS PM，MCCARTHY KK．Management and treatment of staphylococcal mastitis[J]．Vet Clin North Am Food Anim Pract，2003，19(1)：171-185，vii．
[7]	樊利军，张梦泽，韦艺媛，等．奶牛金黄色葡萄球菌乳腺炎小鼠模型的建立[J]．实验动物科学，2011，28(6)：1-6．
[8]	程成，张薇，朱波，等．中药抗常见耐药菌的作用及其机制研究进展[J]．南京中医药大学学报，2019，35(2)：229-233．
[9]	陈依春，李捷，姚欢，等．紫外分光光度法测定市售檀香中总黄酮含量[J]．中国民族民间医药，2020，29(1)：52-54．
[10]	SADER HS，FLAMM RK，JONES RN．Antimicrobial activity of daptomycin tested against Gram-positive pathogens collected in Europe，Latin America，and selected countries in the Asia-Pacific Region (2011)[J]．Diagn Microbiol Infect Dis，2013，75(4)：417-422．
[11]	谢强，林玉桓，苗淑萍，等．香芹酚对大肠杆菌和金黄色葡萄球菌细胞膜的影响[J]．食品工业科技，2014，35(23)：54-58，62．
[12]	李晗．柠檬酸循环调节金黄色葡萄球菌金黄色素的产生[D]．上海：第二军医大学，2012．
[13]	ORHAN G，BAYRAM A，ZER Y，et al．Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis[J]．J Clin Microbiol，2005，43(1)：140-143．
[14]	WENG ZB，ZENG F，ZHU ZH，et al. Comparative analysis of sixteen flavonoids from different parts of Sophora flavescens<\i> Ait. by ultra high-performance liquid chromatography-tandem mass spectrometry[J]. J Pharmaceut Biomed Anal，2018，156：214-220.
[15]	ZHANG L，XU L，XIAO SS，et al．Characterization of flavonoids in the extract of Sophora flavescens Ait．by high-performance liquid chromatography coupled with diode-array detector and electrospray ionization mass spectrometry[J]．J Pharm Biomed Anal，2007，44(5)：1019-1028．
[16]	KUROYANAGI M，ARAKAWA T，HIRAYAMA Y，et al．Antibacterial and antiandrogen flavonoids from Sophora flavescens[J]．J Nat Prod，1999，62(12)：1595-1599．
[17]	MA XC，XIN XL，ZHANG BJ，et al．Structural determination of flavonoids from Sophora flavescens[J]．Magn Reson Chem，2008，46(9)：903-906．
[18]	MA XC，XIN XL，LIU KX，et al．Simultaneous determination of nine major flavonoids in Sophora flavescens by RP-LC[J]．Chromatographia，2008，68：471-474．
[19]	赵艳敏，刘素香，张晨曦，等．基于HPLC-Q-TOF-MS技术的甘草化学成分分析[J]．中草药，2016，47(12)：2061-2068．
[20]	马海娟，高简，张亚丽，等．基于HPLC-MSⁿ的甘草成分快速鉴定及质谱裂解途径研究[J]．中华中医药杂志，2018，33(3)：1120-1123．
[21]	赵鹏宇，焦利卫，张志明，等．UPLC-QTOF-MS研究不同国家甘草渣黄酮成分差异[J]．中国食品添加剂，2017(3)：111-116．
[22]	段天璇，马长华，王文全，等．HPLC-MS法鉴定甘草的指纹图谱[J]．中国药师，2009，12(4)：414-417．
[23]	张鲁，康雪芳，李红丽，等．甘草地上部分黄酮类成分HPLC-MS分析[J]．辽宁中医药大学学报，2018，20(5)：48-51．
[24]	LE MARECHAL C，THIERY R，VAUTOR E，et al．Mastitis impact on technological properties of milk and quality of milk products：a review[J]．Dairy Sci Technol，2011，91(3)：247-282．
[25]	许英民．抗生素替代物的种类及其在养禽上应用[J]．养禽与禽病防治，2016(3)：9-11．
[26]	丁丽军，袁华根．益生菌的作用及安全性研究进展[J]．兽医导刊，2018(2)：252．
[27]	何常明. 苦参和山豆根黄酮类成分及其生物活性的比较研究[D].上海：复旦大学，2010.
[28]	TSUKIYAMA R， KATSURA H， TOKURIKI N， et al. Antibacterial activity of licochalcone A against spore-forming bacteria[J]. Antimicrob Agents Chemother， 2002，46(5)：1226-1230.

Bacteriostasis of Prenylated Flavonoids from Sophora Flavescens<\i> and Flavonoids from Glycyrrhiza Uralensis<\i> Alone and Their Combination on Staphylococcus Aureus<\i> and Experimental Mastitis

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