Study on Fingerprint in TCM Classical Prescription of Qingshang Juantong Decoction

OBJECTIVE To establish a fingerprint of Qingshang Juantong Decoction (QSD)， analyze and identify the common peaks. METHODS HPLC-UV was used to establish fingerprint of QSD; Agilent Poroshell 120 EC-C₁₈ column (150 mm×4.6 mm， 4 μm) was used， eluting with a gradient of methanol-acetonitrile-0.25% glacial acetic acid. Flow rate was 1.0 mL/min， the column temperature was 35 ℃， the injection volume was 5 μL， and the detection wavelength was 355 nm. HPLC-Q-TOF/MS was used for qualitative analysis and the comparison was conducted for verification. RESULTS The methodological study showed that the chromatographic method established met the qualitative research requirements of fingerprinting technology. A total of 34 peaks were shared， and 14 peaks were identified， namely chlorogenic acid， ferulic acid， cynaroside， nodakenin， baicalin， wogonoside， baicalein， wogonin， vitexicarpin， ligustilide， osthole， notopterol， lisoimperatorin， atractylodin. According to the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" (2012 edition)， 10 batches of QSD were investigated， and the similarity was above 0.97. CONCLUSION This method shows high sensitivity， stability and accuracy， which reflects the overall characteristics of Qingshang Juantong Decoction and provides a reliable scientific basis for the establishment of Qingshang Juantong Decoction.