Volume 35 Issue 6
Nov.  2019
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(6): 708-713
WANG Hua-jun, QIAO Chen, ZHAO Rong, YIN Jiang-ning. Study on the Mechanism of Circular-Icaritin Against Glucocorticoid-Induced Osteoporosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 708-713.
Citation: WANG Hua-jun, QIAO Chen, ZHAO Rong, YIN Jiang-ning. Study on the Mechanism of Circular-Icaritin Against Glucocorticoid-Induced Osteoporosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 708-713.
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Study on the Mechanism of Circular-Icaritin Against Glucocorticoid-Induced Osteoporosis

  • Publish Date: 2019-11-10
    Abstract
  • OBJECTIVE To elucidate the reversal effect of cyclic-icaritin(CICT) on prednisolone -induced osteoporosis， and to explore its mechanism of action with BMPs signaling pathway. METHODS Prednisolone-induced osteoporosis model of zebrafish was used. The stained area， cumulative optical density， calcium and phosphorus contents of the skull of zebrafish were observed by fluorescence inverted microscope (×100). qPCR was used to quantitatively detect the expression of Runx 2 and AKP genes. RESULTS Compared with the control group， the cumulative optical density and mineralized area of zebrafish skull significantly decreased (P<0.01)，the Ca and P levels significantly decreased (P<0.01)， the expression of Runx 2 and AKP genes decreased significantly (P<0.01) after incubation with prednisolone (25 μmol/L).After the intervention with different doses of CICT (0.1， 1.0， 10.0 μmol/L)，the cumulative optical density and bone mineralization area of zebrafish skull significantly increased (P<0.01)， and the levels of Ca and P significantly increased (P<0.01). Molecular docking techniques showed that CICT could stably dock with target protein BMPs (BMP-2/BMP-4)， and the docking scores were - 5.49325609 and - 5.99361658， respectively. The expressions of Runx-2 and AKP genes significantly increased (P<0.01). CONCLUSION CICT can reverse glucocorticoid-induced osteoporosis， which plays an anti-osteoporosis role by binding CICT to BMPs protein target and regulating BMPs signaling pathway to promote osteogenic differentiation.

     

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    • References(25)
  • [1]
    FIROOZABADI R， CROSS WW， KRIEG JC， et al. Acetabular fractures in the senior population-epidemiology， mortality and treatments[J]. Arch Bone Jt Surg， 2017， 5(2): 96-102.
    [2]
    BUCKLEY L， GUYATT G， FINK HA， et al. 2017 American college of rheumatology guideline for the prevention and treatment of glucocorticoid-induced osteoporosis [J]. Arthritis Care Res， 2017， 69: 1095-1110.
    [3]
    XI L， DE FALCO P， BARBIERI E， et al. Bone matrix development in steroid-induced osteoporosis is associated with a consistently reduced fibrillar stiffness linked to altered bone mineral quality[J]. Acta Biomater， 2018， 76: 295-307.
    [4]
    WINBLAD L， LARSEN CG， HAKANSSON K， et al. The risk of osteoporosis in oral steroid treatment for nasal polyposis: A systematic review[J]. Rhinology， 2017， 55(3): 195-201.
    [5]
    MAAS BM， WANG J， COONER F， et al. Bone mineral density to assess pediatric bone health in drug development[J]. Ther Innov Regul Sci， 2017， 51(6): 756-760.
    [6]
    FAN S， GAO X， CHEN P， et al. Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway[J]. Life Sci， 2018， 207: 205-211.
    [7]
    LIU S， FANG T， YANG L， et al. Gastrodin protects MC3T3-E1 osteoblasts from dexamethasone-induced cellular dysfunction and promotes bone formation via induction of the NRF2 signaling pathway[J]. Int J Mol Med， 2018， 41(4):2059-2069.
    [8]
    HE H， WANG C， TANG Q， et al. Possible mechanisms of prednisolone-induced osteoporosis in zebrafish larva[J].Biomed Pharmacother， 2018， 101:981-987.
    [9]
    JIANG J， LI J， JIA X. The antiosteoporotic activity of central-icaritin (CIT) on bone metabolism of ovariectomized rats[J]. Molecules， 2014， 19(11):18690-18704.
    [10]
    JIANG J， XIAO S， XU X， et al. Isomeric flavonoid aglycones derived from Epimedii Folium exerted different intensities in anti-osteoporosis through OPG/RANKL protein targets[J]. Int Immunopharmacol， 2018， 62:277-286.
    [11]
    LIU L， TAO W， PAN W， et al. Hydroxysafflor yellow a promoted bone mineralization and inhibited bone resorption which reversed glucocorticoids-induced osteoporosis[J]. Biomed Res Int， 2018， 2018:6762146.
    [12]
    VAREA O， AREVALO MA， GARRIDO JJ， et al. Interaction of estrogen receptors with insulin-like growth factor-I and Wnt signaling in the nervous system[J]. Steroids， 2010， 75(8/9):565-569.
    [13]
    LIU K， JING Y， ZHANG W， et al. Silencing miR-106b accelerates osteogenesis of mesenchymal stem cells and rescues against glucocorticoid-induced osteoporosis by targeting BMP2[J]. Bone， 2017， 97:130-138.
    [14]
    YONGTAO Z， KUNZHENG W， JINGJING Z， et al. Glucocorticoids activate the local renin-angiotensin system in bone: possible mechanism for glucocorticoid-induced osteoporosis[J]. Endocrine， 2014， 47(2):598-608.
    [15]
    LI B， JIANG Y， SUN J， et al. MR spectroscopy for assessing the effects of oxytocin on marrow adipogenesis induced by glucocorticoid in rabbits[J].Acta Radiol， 2016， 57(6):701-707.
    [16]
    KALPAKCIOGLU BB， ENGELKE K， GENANT HK. Advanced imaging assessment of bone fragility in glucocorticoid-induced osteoporosis[J].Bone， 2011， 48(6):1221-1231.
    [17]
    QUARLES LD. Prednisone-induced osteopenia in beagles: variable effects mediated by differential suppression of bone formation[J]. Am J Physiol，1992， 263(1):136-141.
    [18]
    CHAVASSIEUX P， BUFFET A， VERGNAUD P， et al. Short-term effects of corticosteroids on trabecular bone remodeling in old ewes[J].Bone， 1997，20(5):451-455.
    [19]
    SCHOLZ-AHRENS KE， DELLING G， STAMPA B， et al. Glucocorticosteroid-induced osteoporosis in adult primiparous Gottingen miniature pigs: effects on bone mineral and mineral metabolism[J].Am J Physiol Endocrinol Metab， 2007， 293(1):385-395.
    [20]
    PASQUALETTI S， CONGIU T， BANFI G， et al. Alendronate rescued osteoporotic phenotype in a model of glucocorticoid-induced osteoporosis in adult zebrafish scale[J].Int J Exp Pathol， 2015， 96(1):11-20.
    [21]
    BARRETT R， CHAPPELL C， QUICK M， et al. A rapid， high content， in vivo <\i>model of glucocorticoid-induced osteoporosis[J].Biotechnol J， 2006， 1(6):651-655.
    [22]
    DE VRIEZE E， VAN KESSEL MA， PETERS HM， et al. Prednisolone induces osteoporosis-like phenotype in regenerating zebrafish scales[J].Osteoporos Int， 2014， 25(2):567-578.
    [23]
    SALBACH-HIRSCH J， SAMSONOV SA， HINTZE V， et al. Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone[J]. Biomaterials， 2015， 67:335-345.
    [24]
    WEI X， WU W， LI L， et al. Bone morphogenetic proteins 2/4 are upregulated during the early development of vascular calcification in chronic kidney disease[J]. Biomed Res Int， 2018， 2018:8371604.
    [25]
    YIN Q， WANG J， FU Q， et al. CircRUNX2 through has-miR-203 regulates RUNX2 to prevent osteoporosis[J]. J Cell Mol Med， 2018，22(12):6112-6121.
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