Volume 35 Issue 1
Jan.  2019
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(1): 85-89
ZHOUJia, CAIHao, DUANYu, PEIKe, XUYang-yang, LIUJing, NIUMin-jie, ZHANGYa-ting. Simultaneous Determination of 16 Main Chemical Components in Aqueous Extract of Sinisan Based on UHPLC-QqQ-MS Technology[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 85-89.
Citation: ZHOUJia, CAIHao, DUANYu, PEIKe, XUYang-yang, LIUJing, NIUMin-jie, ZHANGYa-ting. Simultaneous Determination of 16 Main Chemical Components in Aqueous Extract of Sinisan Based on UHPLC-QqQ-MS Technology[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 85-89.
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Simultaneous Determination of 16 Main Chemical Components in Aqueous Extract of Sinisan Based on UHPLC-QqQ-MS Technology

  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 2.

    Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 3.

    Institute of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine, Taiyuan, 030024, China

  • Publish Date: 2019-01-10
    Abstract
  • OBJECTIVE A method for simultaneous determination of 16 chemical components in aqueous extract of Sinisan was established. METHODS UHPLC-QqQ-MS technology was employed. Scan was conducted in negative ion mode and quantitative determination was carried out by MRM paired ions. RESULTS Saikosaponina A， saikosaponina B2， saikosaponina C， saikosaponina D， hesperitin， cianidanol， liquiritin， glycyrrhizic acid， liquiritigenin， chlorogenic acid， gallic acid， paeoniflorin， albiflorin， isoliquiritigenin， isochlorogenic acid A， and hesperidin all have good responses in negative ion mode. CONCLUSION The established method is specific， rapid， sensitive， accurate， and reliable， which can simultaneously determine contents and accurately give mass information of compound to achieve the effects of component identification， and also it can provide references for quality control and evaluation of aqueous extract of Sinisan.

     

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