Volume 35 Issue 1
Jan.  2019
Turn off MathJax
Article Contents
Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(1): 78-84
JIJian-jian, YANGRui, XIETong, SHENCun-si, LINLi-li, WANGShou-chuan, SHANJin-jun. Lipomics Study of Jinxin Oral Liquid for RSV Infection in Mice Based on UHPLC-Q-Exactive Orbitrap/MS[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 78-84.
Citation: JIJian-jian, YANGRui, XIETong, SHENCun-si, LINLi-li, WANGShou-chuan, SHANJin-jun. Lipomics Study of Jinxin Oral Liquid for RSV Infection in Mice Based on UHPLC-Q-Exactive Orbitrap/MS[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 78-84.
shu

Lipomics Study of Jinxin Oral Liquid for RSV Infection in Mice Based on UHPLC-Q-Exactive Orbitrap/MS

  • 1.

    Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 2.

    Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • Publish Date: 2019-01-10
    Abstract
  • OBJECTIVE Alipidomic method was applied to determine the lipidome in serum of mice based on high-resolution mass spectrometry (Q-Exactive Orbitrap). We investigated the changes of lipids in serum of mice with RSV to explore the regulation of Jinxin oral liquid on lipid metabolism caused by RSV. METHODS The serum samples from the blank group， the RSV model group and Jinxin oral liquid group mice were collected， and the lipids of these samples were extracted and further analyzed by high-resolution mass spectrometry. Multivariate statistical methods such as principal component analysis (PCA) and cluster analysis (CA) were used to analyze the lipid profiles， and the Kruskal-Wallis test and fold change were performed to find the differential lipids. RESULTS The lipids of serum between the blank control group and the RSV model group could be well distinguished， RSV infection in mice with metabolic abnormalities of lipids， the specific performance of the PC， PE was significantly upregulated， Jinxin oral liquid have a certain role in these lipids CONCLUSION RSV infection caused disturbances of lipid in vivo， and Jinxin oral liquid has a certain regulatory effect on the lipid metabolism disorder caused by RSV model.

     

    • FullText(HTML)
  • loading
    • References(22)
  • [1]
    ZHOU N， LI W， REN L， et al. An Interaction of LPS and RSV Infection in Augmenting the AHR and Airway Inflammation in Mice[J]. Inflammation， 2017， 40(5):1643-1653.
    [2]
    GROVES HT， CUTHBERTSON L， JAMES P， et al. Respiratory disease following viral lung infection alters the murine gut microbiota[J]. Front Immunol， 2018， 9:182.
    [3]
    孟欣， 单进军， 谢彤， 等. 基于血浆代谢组学分析金欣口服液治疗小鼠RSV肺炎的作用机制[J]. 中国实验方剂学杂志， 2016，22(17):71-76.
    [4]
    MARI N， YOJI N， MOORE ML， et al. Phosphatidylglycerol provides short-term prophylaxis against respiratory syncytial virus infection[J]. J Lipid Res， 2013， 54(8):2133.
    [5]
    杜丽娜， 单进军， 徐建亚，等. 金欣口服液不同化学部位对呼吸道合胞病毒感染的影响[J]. 南京中医药大学学报， 2013， 29(2):165-168.
    [6]
    SHEN CS， ZHANG ZG， XIE T， et al.Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway[J]. Biomed Pharmacother， 2018， 103:1376.
    [7]
    彭琳秀， 陈良慧， 狄留庆， 等. 基于UPLC/LTQ-Orbitrap-MS技术的复方祖师麻片抗类风湿关节炎的血浆代谢组学研究[J]. 中草药， 2017， 48(10):1964-1970.
    [8]
    FESSLER MB， SUMMER RS. Surfactant lipids at the host-environment interface: Metabolic sensors， suppressors， and effectors of inflammatory lung disease[J]. Am J Respir Cell Mol Biol， 2016， 54(5):624-635.
    [9]
    钱文娟， 杨瑞， 谢彤， 等. 蒲地蓝消炎口服液治疗甲型H1N1流感病毒性肺炎的GC-MS代谢组学研究[J]. 中草药， 2018，49(10):2258-2264.
    [10]
    漆欣， 李菊莲， 王银平， 等. 脂质代谢组学在针灸研究中的应用展望[J]. 中医药导报， 2018，23(14):93-95，104.
    [11]
    李佳曦， 汪受传， 徐建亚， 等. 金欣口服液含药血清及其有效单体白藜芦醇对RSV的RNA聚合酶转录活性的影响[J]. 南京中医药大学学报， 2011，27(1):36-39.
    [12]
    陈争光，汪受传，徐建亚，等.金欣口服液对RSV感染BALB/c小鼠TLR3信号转导通路负调控因子SOCS1表达的调控作用[J].中国中西医结合杂志，2014，34(12):1499-1506.
    [13]
    TSUGAWA H， CAJKA T， KIND T， et al. MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis[J]. Nature methods， 2015， 12(6):523-526.
    [14]
    DEFELICE BC， MEHTA SS， SAMRA S， et al. Mass spectral feature list optimizer (MS-FLO): a tool to minimize false positive peak reports in untargeted liquid chromatography-mass spectroscopy (LC-MS) data processing[J]. Analyt Chem， 2017， 89(6):3250-3255.
    [15]
    NUMATA M， CHU HW， DAKHAMA A， et al. Pulmonary surfactantphosphatidylglycerol inhibits respiratory syncytial virus-induced inflammation and infection.[J]. Proc Natl Acad Sci USA， 2010， 107(1): 320-325.
    [16]
    BARREIRA ER， PRECIOSO AR， BOUSSO A. Pulmonary surfactant in respiratory syncytial virus bronchiolitis: The role in pathogenesis and clinical implications[J]. Pediatr Pulmonol， 2011， 46(5):6.
    [17]
    谢露， 尹晓娟. 新生儿肺表面活性物质研究新进展[J]. 中国儿童保健杂志， 2012， 20(3):235-237.
    [18]
    HITE RD， SEEDS MC， JACINTO RB， et al.Lysophospholipid and fatty acid inhibition of pulmonary surfactant: non-enzymatic models of phospholipase A2 surfactant hydrolysis.[J]. BBA - Biomembranes， 2005， 1720(1):14-21.
    [19]
    杜丽娜， 单进军， 谢彤， 等. 基于液相质谱联用的黄芩水提液抗病毒效应的脂质组学研究[J]. 中华中医药杂志， 2015，30(5):1728-1733.
    [20]
    陈华， 于海洲， 彭创业. HPLC-ELSD法同时测定丙泊酚注射液中溶血磷脂酰胆碱和溶血磷脂酰乙醇胺的含量[J]. 药物分析杂志， 2014，34(3):442-446.
    [21]
    林丽丽，谢彤，汪受传，等.代谢组学在儿科疾病中的应用[J].南京中医药大学学报，2017，33(2):182-186.
    [22]
    ENHORNING G. Surfactant in airway disease[J]. Chest， 2008， 133(4):975-980.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Get Citation
    PDF
    XML

    Article Metrics

    Article views (780) PDF downloads(613) Cited by()
    Proportional views
    Related

    Copyright © Journal of Nanjing University of traditional Chinese Medicine

    Sponsored by：Nanjing University of traditional Chinese MedicineAddress：No.138 xianlin Avenue, Qixia District, Nanjing cityChina Pos：210023

    Tel：025-85811935Email：xb@njucm.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return