Volume 35 Issue 1
Jan.  2019
Turn off MathJax
Article Contents
Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(1): 73-77
WANGXu, SHAOXin, XUHai-yan, YELi-fang, XUXi-ru, YANGXiao-qing. Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 73-77.
Citation: WANGXu, SHAOXin, XUHai-yan, YELi-fang, XUXi-ru, YANGXiao-qing. Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 73-77.
shu

Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats

  • 1.

    Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China

  • 2.

    The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, China

  • 3.

    Basic Medical College, Xuzhou Medical University, Xuzhou, 221004, China

  • Publish Date: 2019-01-10
    Abstract
  • OBJECTIVE To observe the effect of Tangzhiqing on the endoplasmic reticulum stress related factors GPR78， CHOP and Caspase-12 in hippocampus of diabetic rats， and to explore its mechanism of improving cognitive function in diabetic rats. METHODS The model of type 2 diabetes mellitus rats was established by intraperitoneal injection of low-dose of streptozotocin combined with high-sugar and high-fat diet， After 8 weeks of intervention with high， medium and low-dose of Tangzhiqing， serum fasting glucose (FPG) and fasting insulin (FINS) were detected by ELISA， the index of insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated; the expression levels of endoplasmic reticulum stress related genes GPR78， CHOP and Caspase-12 mRNA and protein in hippocampus were detected by qPCR and Western blot assay. RESULTS Different dosages of Tangzhiqing significantly decreased FPG and HOMA-IR ( P <0.01); except the low dose group， Tangzhiqing significantly improved ISI ( P <0.01); no significant difference existed in FPG level among the 3 groups ( P >0.05). Compared to the model group， the relative expression of GRP78 mRNA and protein in the 3 groups of Tangzhiqing increased significantly， and the expression of CHOP and Caspase-12 mRNA in these 3 groups decreased ( P <0.01). CHOP and Caspase-12 in middle and high dose group decreased significantly ( P <0.01). CONCLUSION Tangzhiqing can control blood sugar， improve insulin resistance， increase insulin sensitivity and regulate the expression of GRP78， CHOP， Caspase-12 in hippocampus.

     

    • FullText(HTML)
  • loading
    • References(16)
  • [1]
    BIESSELS GJ， DEARY IJ， RYAN CM. Cognition and diabetes: a lifespan perspective[J]. Lancet Neurol，2008，7(2):184-190.
    [2]
    VAN DEN BERG E， KLOPPENBORG RP， KESSELS RP， et al. Type 2 diabetes mellitus， hypertension， dyslipidemia and obesity: A systematic comparison of their impact on cognition[J]. Biochim Biophys Acta，2009，1792(5):470-481.
    [3]
    GUDALA K， BANSAL D， SCHIFANO F， et al. Diabetes mellitus and risk of dementia: A meta-analysis of prospective observational studies[J]. J Diabetes Investig，2013，4(6):640-650.
    [4]
    STRACHAN MW， REYNOLDS RM， MARIONI RE， et al. Cognitive function， dementia and type 2 diabetes mellitus in the elderly[J]. Nat Rev Endocrinol，2011，7(2):108-114.
    [5]
    XU W， CARACCIOLO B， WANG HX， et al. Accelerated progression from mild cognitive impairment to dementia in people with diabetes[J]. Diabetes，2010，59(11):2928-2935.
    [6]
    杨晓青，王旭，邵鑫. 糖脂清方通过保护海马神经元对提高2型糖尿病大鼠学习记忆能力的影响[J]. 中国实验方剂学杂志，2018，24(10):360-365.
    [7]
    蒯美玉，马志，孙鑫，等. 桑酮碱改善2型糖尿病大鼠的糖脂代谢[J]. 中成药，2017，39(1):21-26.
    [8]
    袁有才，问莉娜，曹旸，等. 糖尿病认知功能障碍的中医病因病机研究进展[J]. 光明中医，2016，31(24):3687-3690.
    [9]
    SUN FC， WEI S， LI CW， et al. Localization of GRP78 to mitochondria under the unfolded protein response[J]. Biochem J，2006，396(1):31-39.
    [10]
    CHEN X， LI M， CHEN D， et al. Autophagy induced by calcium phosphate precipitates involves endoplasmic reticulum membranes in autophagosome biogenesis[J]. PLoS ONE，2012，7(12):e52347.
    [11]
    BOUMAN L， SCHLIERF A， LUTZ AK， et al. Parkin is transcriptionally regulated by ATF4: evidence for an interconnection between mitochondrial stress and ER stress[J]. Cell Death Differ，2011，18(5):769-782.
    [12]
    PUTHALAKATH H， O'REILLY LA， GUNN P， et al. ER stress triggers apoptosis by activating BH3-only protein Bim[J]. Cell，2007，129(7):1337-1349.
    [13]
    ROY S， SHAROM JR， HOUDE C， et al. Confinement of caspase-12 proteolytic activity to autoprocessing[J]. Proc Natl Acad Sci USA，2008，105(11):4133-4138.
    [14]
    WENG S， ZHU X， JIN Y， et al. Protective effect of erythropoietin on myocardial infarction in rats by inhibition of caspase-12 expression[J]. Exp Ther Med，2011，2(5):833-836.
    [15]
    CHEN YH， WU XD， YAO ST， et al. Calcineurin is involved in cardioprotection induced by ischemic postconditioning through attenuating endoplasmic reticulum stress[J]. Chin Med J (Engl)，2011，124(20):3334-3340.
    [16]
    梁丽娜. 滋补脾阴方药对脾阴虚糖尿病脑病大鼠海马内质网应激影响的研究[D]. 大连:大连医科大学，2009.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Get Citation
    PDF
    XML

    Article Metrics

    Article views (720) PDF downloads(798) Cited by()
    Proportional views
    Related

    Copyright © Journal of Nanjing University of traditional Chinese Medicine

    Sponsored by：Nanjing University of traditional Chinese MedicineAddress：No.138 xianlin Avenue, Qixia District, Nanjing cityChina Pos：210023

    Tel：025-85811935Email：xb@njucm.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return