Volume 35 Issue 1
Jan.  2019
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(1): 63-67
LIUMin, LIUChun-liang, LIANGGuo-qiang, YAOFei, ZHANGXiao-die, CHENTing, YUANYa-qin, WUShi-liang. Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 63-67.
Citation: LIUMin, LIUChun-liang, LIANGGuo-qiang, YAOFei, ZHANGXiao-die, CHENTing, YUANYa-qin, WUShi-liang. Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 63-67.
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Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells

  • 1.

    Affiliated Suzhou Hospital of TCM to Nanjing University of Chinese Medicine, Suzhou, 215009, China

  • 2.

    School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, China

  • Publish Date: 2019-01-10
    Abstract
  • OBJECTIVE Based on glycosyltransferase β3GnT8， to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang. METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay. By interference and transfection of β3GnT8， high and low expressed β3GnT8 MDA-MB-231/TAX cell lines were obtained. Western blot was used to detect the protein expression of β3GnT8， and drug resistance protein BCRP and P-gp. Lectin flow cytometry was used to detect β3GnT8 fluorescence intensity on β3GnT8 transfected MDA-MB-231/TAX cells. RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells， and the inhibitory rate was 50.16% after 72 h. Zhuidu Fang significantly downregulated the protein expression of β3GnT8， BCRP and P-gp. The protein expression of β3GnT8 showed a positive correlation with BCRP and P-gp. CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines， which is associated with the downregulation of the expression of β3GnT8， BCRP and P-gp.

     

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