Volume 35 Issue 1
Jan.  2019
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2019  >  35(1): 47-52
YANGZhi-chao, YANGJian-jun, MAJun, HUXiang-nong, LIUYuan, ZHANGLi. Effect of Modifie Drehmanniae Prescription on TGF-β1-CTGF Signaling Pathway to Improve Renal Interstitial Fibrosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 47-52.
Citation: YANGZhi-chao, YANGJian-jun, MAJun, HUXiang-nong, LIUYuan, ZHANGLi. Effect of Modifie Drehmanniae Prescription on TGF-β1-CTGF Signaling Pathway to Improve Renal Interstitial Fibrosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(1): 47-52.
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Effect of Modifie Drehmanniae Prescription on TGF-β1-CTGF Signaling Pathway to Improve Renal Interstitial Fibrosis

  • 1.

    The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 2.

    Department of Urology Surgery, Chinese and Western Medicine Hospital of Jiangsu Province, Nanjing, 210028, China

  • 3.

    Department of Kidney Surgery, Chinese Medicine Hospital of Yixing City, Yixing, 214200, China

  • 4.

    Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China

  • Publish Date: 2019-01-10
    Abstract
  • OBJECTIVE To study the effect of modified Rehmannia Recipe on renal interstitial fibrosis in rats with partial ureteral obstruction (PUUO) and to explore its possible mechanism. METHODS Eighty SD rats were randomly divided into control group， model group， low dose group and high dose group ( n =20). In the model group， the model of PUUO was established by lumbar large muscle tube embedding method. Only the left kidney was removed in the control group.High dose group (2 mL/d) and low dose group (1 mL/d) were treated with Modified rehmannia decoction， the control group and the model group were given the same amount of normal saline. The levels of serum creatinine (SCr)， urea nitrogen (BUN) and 24 h urinary protein were determined in each group. Blood samples were collected from each group at 4th week and 8th week after modeling. Measurement of renal tissue size， renal cortical thickness. The renal histopathological changes were observed by staining with HE and Masson， and the renal fibrosis was observed. The expressions of transforming growth factor-β1 (TGF-β1)， connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in renal tissue were detected by immunohistochemistry. The expression of TGF-β1 and CTGF mRNA was detected by qPCR. RESULTS At 4th week， inflammatory cell infiltration， local focal necrosis and necrosis were observed in the model group， and more collagen fibrosis was formed. Compared with the model group， the pathological changes of the high and low dose group were not obvious， and only a small interstitial fibrosis was widened. After 8 weeks: model group lymphocyte infiltration， glomerular necrosis， the structure completely changed. Interstitial fibrous hyperplasia. In the high and low dose group， the infiltration of inflammatory cells was lighter than that of the model group， the staining of the collagen was lighter and the high dose group had the smallest change. The average positive expression rate of TGF-β1， CTGF and α-SMA protein was not significantly different between the two groups at 4th week compared with the model group. At the 8th week， the two drug treatment groups had Significant difference ( P <0.01). Comparison of TGF-β1， CTGF mRNA expression was found when the low-dose group 4 weeks with model group had no differences as compared to high dose group and the model group were different ( P <0.05) as compared to 8th week. CONCLUSION Modified rhubarb may reduce the expression of TGF-β1-CTGF and down-regulate the expression of α-SMA， reduce the pathological changes of renal fibrosis and delay the development of renal fibrosis.

     

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    • References(27)
  • [1]
    KHWAJA A，EL KOSSI M，FLOEGE J，et al． The management of ckd: A look into the future[J]． Kidney Int，2007，72(11)： 1316-1323．
    [2]
    LIU Y． Renal fibrosis: New insights into the pathogenesis and therapeutics[J]． Kidney Int，2006，69(2)： 213-217．
    [3]
    JI NF，YAO LS，LI Y， et al． Polysaccharide of cordyceps sinensis enhances cisplatin cytotoxicity in non-small cell lung cancer H157 cell line[J]． Integr Cancer Ther，2011，10(4)： 359-367.
    [4]
    LIU Y． New insights into epithelial-mesenchymal transition in kidney fibrosis[J]．J Am Soc Nephrol，2010，21(2)： 212-222．
    [5]
    车丽双. TGF-β1与CTGF在肾间质纤维化中的作用[J].医学综述，2013，19（4）:624-626.
    [6]
    BOOR P， SEBEKOVA K， OSTENDORF T， et al. Treatment targets in renal fibrosis[J]. Nephrol Dial Transplant， 2007， 22(12):3391-3407.
    [7]
    TURNER JM， BAUER C， ABRAMOWITZ MK， et al. Treatment of chronic kidney disease[J].Kidney Int， 2012， 81(4):351-362.
    [8]
    徐剑，袁红伶，倪凯，等.翠云草对单侧输尿管梗阻大鼠肾间质纤维化抑制作用研究[J].上海交通大学学报（医学版），2016， 36 (12)：1689-1696.
    [9]
    LI Y， GE Y， LIU FY， et al. Norcantharidin， a protective therapeutic agent in renal tubulointerstitial fibrosis[J]. Mol Cell Biochem， 2012， 361(1/2):79-83.
    [10]
    杨关天，杨建军，姚茂银.输尿管部分梗阻与肾小管间质纤维化[J]. 医学研究生学报，2006，3:252.
    [11]
    BOOR P，OSTENDORF T，FLOEGE J. Renal fibrosis: novel insights into mechanisms and therapeutic targets[J]． Nat Rev Nephrol，2010，6(11)： 643-656.
    [12]
    VERNON MA， MYLONAS KJ， HUGHES J. Macrophages and renal fibrosis[J]. Semin Nephrol， 2010， 30(3):302-317.
    [13]
    MENG XM， NIKOLIC-PATERSON DJ， LAN HY. Inflammatory processes in renal fibrosis[J]. Nat Rev Nephrol， 2014， 10(9):493-503.
    [14]
    ZEISBERG M，NEILSON EG. Mechanisms of tubulointerstitial fibrosis[J]. J Am Soc Nephrol，2010，21 (11)：1819-1834.
    [15]
    QI W，CHEN X，PORONNIK P，et al． The renal cortical fibroblast in renal tubulointerstitial fibrosis[J]．Int J Biochem Cell Biol，2006，38(1)：1-5.
    [16]
    SILVA GE，COSTA RS，RAVINAL RC，et al． Mast cells，TGF-beta1 and alpha-SMA expression in IgA nephropathy[J]．Dis Markers，2008，24(3):181-190．
    [17]
    RISER BL， NAJMABADI F， PERBAL B， et al. CCN3(NOV) is a negative regulator of CCN2 (CTGF) and a novel endoge-nous inhibitor of the fibrotic pathway in an in vitro model of renal disease[J]. Am J Pathol， 2009， 174(5):1725-1734.
    [18]
    WANG YN， LIN C， REN Q， et al． Astragaloside effect on TGF-β1，SMAD2/3，and α-SMA expression in the kidney tissues of diabetic KKAy mice[J]． Int J Clin Exp Pathol，2015，8(6):6828-6834．
    [19]
    SAMUEL A， BLACK JR， TRACKMAN PC. Transforming growth factor-β1 (TGFβ1) stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibro-blasts through a RhoA independent， Rac1/Cdc42-depend-ent mechanism: Statins with forskolin block TGFβ1-in-duced CCN2/CTGF expression[J]. Biol Chem， 2008， 283(16):10835-10847.
    [20]
    HAYDONT V， RISER BL， AIGUEPERSE J， et al. Specific signals involved in the long-term maintenance of radiation-in-duced fibrogenic differentiation: a role for CCN2 and low concentration of TGF-beta1[J]. Am J Physiol Cell Physiol， 2008， 294(6):1332-1341.
    [21]
    TURNER JM， BAUER C， ABRAMOWITZ MK， et al. Treatment of chronic kidney disease[J]. Kidney Int， 2012， 81(4):351-362.
    [22]
    吴小南，吴异兰，陈洁. 川芎嗪对高糖诱导的人肾小管上皮细胞转分化的影响[J].中国全科医学，2009，12(5)：843-846．
    [23]
    李珊珊，金银萍，姚春林，等. 人参多糖的结构与活性研究进展[J]．中国中药杂志，2014，39（24）：4709-4714.
    [24]
    FAN GW，ZHANG Y，JIANG X，et al. Anti-inflammatory activity of baicalein in LPS-stimulated RAW264.7 macrophages via estrogen receptor and NF-kappaB-dependent pathways[J]．Inflammation，2013，36: 1584-1591．
    [25]
    YANG YZ， TANG YZ， LIU YH． Wogonoside displays anti-inflammatory effects through modulating inflammatory mediator expression using RAW264．7 cells[J]． J Ethnopharmacol，2013，148:271-276．
    [26]
    KUO JJ， WANG CY， LEE TF，et al． Paeoniae Radix reduces PDGF-stimulated hepatic stellate cell migration[J]． Planta Med，2012，78(4)： 341-348．
    [27]
    HU ZT，GAO SL，QIN F，et al． Effect of paeoniflorin on TGF-β /smad signal transduction pathway in rats with radiation-induced hepatic fibrosis[J]． Pharm J Chin PLA，2012，28(4)： 283-288．
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