Volume 40 Issue 4
Apr.  2024
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2024  >  40(4): 359-368
YANG Bin, TANG Yu, SHI Chen, SHAN Jinjun. Lipidomics Study of Huashi Baidu Granules in the Treatment of Respiratory Syncytial Virus Pneumonia Model Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(4): 359-368. doi: 10.14148/j.issn.1672-0482.2024.0359
Citation: YANG Bin, TANG Yu, SHI Chen, SHAN Jinjun. Lipidomics Study of Huashi Baidu Granules in the Treatment of Respiratory Syncytial Virus Pneumonia Model Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(4): 359-368. doi: 10.14148/j.issn.1672-0482.2024.0359
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Lipidomics Study of Huashi Baidu Granules in the Treatment of Respiratory Syncytial Virus Pneumonia Model Mice

doi: 10.14148/j.issn.1672-0482.2024.0359
  • 1.

    Institute of Pediatrics, Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Children's Health and Chinese Medicine, Nanjing 210023, China

  • 2.

    Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • Received Date: 2023-10-07
    Available Online: 2024-04-24
    Abstract
  •   OBJECTIVE  To investigate the therapeutic effect of Huashi Baidu Granules on respiratory syncytial virus (RSV) pneumonia model mice and its effect on lung lipid metabolism.  METHODS  BALB/c mice were randomly divided into control group, model group, Ribavirin group, Dexamethasone group, low dose group of Huashi Baidu Granules and high dose group of Huashi Baidu Granules. Lung tissue samples of mice in each group were collected to observe pathological changes and detect mRNA expression levels of RSV-F, RSV-G, IL-1β, IL-6 and TNF-α genes. The lipid components of lung tissue were extracted and analyzed by ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high-resolution mass spectrometry (UPLC-Q Exactive Orbitrap MS) to find the changes in lipid metabolism.  RESULTS  The model group exhibited pulmonary interstitial thickening and inflammatory infiltration compared to the control group, the mRNA levels of viral load and inflammatory factors in lung tissue such as IL-1β and TNF-α were significantly increased (P < 0.001). Moreover, the model group exhibited significant abnormalities in lipid metabolism, including disorders in phosphatidylserine (PS), phosphatidylglycerol (PG), sphingomyelin (SM), ceramide (Cer), triglyceride (TG), diglyceride (DG), fatty acid (FA), ether phosphatidylcholine (PC O), ether lysophosphatidylcholine (LPC O). However, after the treatment of Huashi Baidu Granules (25.12 g·kg-1·d-1), the above indexes showed a significant improvement.  CONCLUSION  Huashi Baidu Granules can treat RSV pneumonia by regulating lipid metabolism, reducing viral load and alleviating inflammatory response in lung tissue.

     

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