Volume 40 Issue 3
Mar.  2024
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2024  >  40(3): 261-267
WANG Yan, GAO Tiantian, WANG Ziying, CHEN Gang, TAO Weiwei. Effects of Yueju Pill on Depressive Behavior, Functional Dyspepsia and PACAP/PAC1-R Expression in CUMS Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(3): 261-267. doi: 10.14148/j.issn.1672-0482.2024.0261
Citation: WANG Yan, GAO Tiantian, WANG Ziying, CHEN Gang, TAO Weiwei. Effects of Yueju Pill on Depressive Behavior, Functional Dyspepsia and PACAP/PAC1-R Expression in CUMS Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(3): 261-267. doi: 10.14148/j.issn.1672-0482.2024.0261
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Effects of Yueju Pill on Depressive Behavior, Functional Dyspepsia and PACAP/PAC1-R Expression in CUMS Mice

doi: 10.14148/j.issn.1672-0482.2024.0261
  • 1.

    School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, China

  • Received Date: 2023-11-09
    Available Online: 2024-03-15
    Abstract
  •   OBJECTIVE  To explore the effects of Yueju Pill on depression and gastrointestinal function in depressed co-morbid functional dyspepsia mice.  METHODS  C57BL/6J mice were randomly divided into control group, model group, Yueju Pill low-dose group, Yueju Pill high-dose group and positive drug group. A co-morbidity model was constructed using chronic unpredictable mild stress (CUMS), and the mice were assessed for depression-like behaviour and neuronal damage by behavioural tests and Nissl staining; gastrointestinal function was assessed by HE staining of gastric and intestinal tissues, gastric emptying rate, and small intestinal propulsive rate; PACAP, VIP, IL-6, TNF-α, and BDNF expression were detected by ELISA; PAC1-R, Vipr1, and Vipr2 protein expression were detected by protein immunoblotting.  RESULTS  Mice in the model group showed depression-like behaviour, reduced number of hippocampal nidus, slowed gastrointestinal motility, elevated serum inflammatory factors IL-6 and TNF-α (P < 0.05, P < 0.01), and reduced expression of PACAP, VIP, and BDNF (P < 0.05, P < 0.01), The PAC1-R, VPAC1-R, VPAC2-R expression decreased in the hippocampus and gastric sinus, duodenal tissue (P < 0.05, P < 0.01). Compared with the model group, the low and high dose groups of Yueju Pill improved the above indexes except for Vipr1 and Vipr2 (P < 0.05, P < 0.01).  CONCLUSION  Yueju Pill can reduce inflammatory factors through PACAP/PAC1-R, increase the level of BDNF, and improve the depression-like behaviour and gastrointestinal dysfunction in CUMS mice.

     

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