Volume 40 Issue 2
Feb.  2024
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2024  >  40(2): 167-173
XIE Huan, SONG Xuanfei, ZHOU Bin, LIU Yao, YANG Fangfang, CHEN Yinglong, ZHU Yan, ZHANG Yongping. Study on the Multi-Component Skin Pharmacokinetics of Crossbow Microemulsion after Transdermal Administration Based on Microdialysis Combined with UPLC[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(2): 167-173. doi: 10.14148/j.issn.1672-0482.2024.0167
Citation: XIE Huan, SONG Xuanfei, ZHOU Bin, LIU Yao, YANG Fangfang, CHEN Yinglong, ZHU Yan, ZHANG Yongping. Study on the Multi-Component Skin Pharmacokinetics of Crossbow Microemulsion after Transdermal Administration Based on Microdialysis Combined with UPLC[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(2): 167-173. doi: 10.14148/j.issn.1672-0482.2024.0167
shu

Study on the Multi-Component Skin Pharmacokinetics of Crossbow Microemulsion after Transdermal Administration Based on Microdialysis Combined with UPLC

doi: 10.14148/j.issn.1672-0482.2024.0167
  • 1.

    School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

  • 2.

    Guizhou Institute of Engineering and Technology for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China

  • 3.

    National Engineering and Technology Research Center of Miao Medicine, Guiyang 550025, China

  • 4.

    College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

  • 5.

    School of Traditional Chinese Medicine, Guizhou Health Vocation College, Tongren 554300, China

  • Received Date: 2023-07-28
    Available Online: 2024-02-26
    Abstract
  •   OBJECTIVE  To investigate the pharmacokinetic characteristics of crossbow microemulsion in rabbit skin.  METHODS  The concentrations of chlorogenic acid, benzoyl new aconitine, periplocin and new aconitine were determined by UPLC. The effects of temperature, perfusion speed and mass concentration on the recovery rate of the probe were investigated by incremental and decrement methods, the changes of multi-component content in the dialysate at different time points after transdermal administration of crossbow microemulsion were determined by UPLC, and DAS 2.0 software was used to process the data.  RESULTS  The recovery rate of the probe increased with increasing temperature, and the higher the flow rate, the lower the recovery rate. The pharmacokinetic parameters of chlorogenic acid, benzoyl new aconitine, periplocin and new aconitine in microemulsion Cmax were (3.24±0.39) (1.04±0.29) (4.78 ± 2.15) (2.86 ± 0.55) μg·mL-1, respectively, and in AUC0-∞ were (52.42 ± 30.84) (7.70 ± 3.73) (42.23 ± 13.93) (22.68 ± 12.51) h·mg·mL-1, respectively.  CONCLUSION  A real-time in vivo microdialysis sampling technique was first used and a UPLC detection technique was established to study the skin pharmacokinetics of multi-components of crossbow microemulsion after transdermal administration, which provides a reference for the follow-up investigation and application of crossbow microemulsion.

     

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