Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes

LYU Ting-mei; LIU Heng; WANG Xi-tong; WANG Yu-tong; CHEN Zhi-peng

doi:10.14148/j.issn.1672-0482.2023.1211

Volume 39 Issue 12

Dec. 2023

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LYU Ting-mei, LIU Heng, WANG Xi-tong, WANG Yu-tong, CHEN Zhi-peng. Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(12): 1211-1223. doi: 10.14148/j.issn.1672-0482.2023.1211

Citation:

LYU Ting-mei, LIU Heng, WANG Xi-tong, WANG Yu-tong, CHEN Zhi-peng. Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(12): 1211-1223. doi: 10.14148/j.issn.1672-0482.2023.1211

Citation:

LYU Ting-mei, LIU Heng, WANG Xi-tong, WANG Yu-tong, CHEN Zhi-peng. Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(12): 1211-1223. doi: 10.14148/j.issn.1672-0482.2023.1211

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Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes

doi: 10.14148/j.issn.1672-0482.2023.1211

LYU Ting-mei^{1, 2
,},
LIU Heng^{1, 2},
WANG Xi-tong^{1, 2},
WANG Yu-tong^{1
,
,},
CHEN Zhi-peng^{1, 2
,
,}

1.
Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
2.
Yunnan Provincial Key Laboratory of Insect Biomedicine Research and Development, Dali University, Yunnan Provincial Engineering Research Center for the Development and Comprehensive Utilization of Medicinal Insect Resources, Dali 671000, China

Received Date: 2023-08-18
Available Online: 2023-12-20

Abstract

Abstract

OBJECTIVE This study aimed to explore the optimal process for preparing long-acting liposomes loaded with cockroach extract (CE-Lip) and to evaluate their therapeutic efficacy against ulcerative colitis (UC). METHODS The CE-Lip was prepared using a thin film dispersion method. Orthogonal experiments were conducted to optimize the formulation and freeze-drying process of CE-Lip. Morphological characterization and in vitro release rate assessment were performed. The therapeutic efficacy of CE-Lip was evaluated using a mouse model of UC. RESULTS The optimal preparation process involved a cholesterol-phospholipid mass ratio of 1 ∶ 5, an extract-phospholipid mass ratio of 1 ∶ 4, hydration time of 0.75 h, hydration volume of 3 mL, and hydration temperature of 30 ℃. The optimal freeze-drying process utilized a mixture of 5% mannitol and 10% trehalose as protective agents. The resulting CE-Lip exhibited an average particle size of (276.7±5.6) nm, with a vesicular structure. The stability of CE-Lip after freeze-drying was excellent, and its in vitro release rate was significantly lower than that of the raw material. Pharmacological results demonstrated that CE-Lip significantly reduced DAI scores, colon length, CMDI scores, TNF-α expression, and HS scores in UC mice, while increasing EGF expression and mucin expression in goblet cells. CONCLUSION A simple, stable, and reproducible process for preparing CE-Lip is established. The resulting CE-Lip exhibits uniform size distribution, good stability, and sustained release effects. Compared to conventional formulations, CE-Lip significantly enhanced the therapeutic effect of CE against UC in mice. This study provides a foundation for further development and research of CE-Lip.
- cockroach extract,
- liposomes,
- orthogonal experiment,
- ulcerative colitis

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References(31)

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Preparation, Characterization, and Anti-Ulcerative Colitis Efficacy of Cockroach Extract Liposomes

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