Volume 39 Issue 12
Dec.  2023
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2023  >  39(12): 1189-1197
LIU Jia-li, XIE Shun-ming, DAI Hui, CHEN Xing-rui, JI Li, LI Shao-liang, QIAN Hai-hua, ZHANG Dan. Study on the Changes in Brain Function Characteristics of Chaihu Tongbian Decoction in Rats with Slow Transit Constipation and Depression Based on Resting-State Functional Magnetic Resonance Imaging[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(12): 1189-1197. doi: 10.14148/j.issn.1672-0482.2023.1189
Citation: LIU Jia-li, XIE Shun-ming, DAI Hui, CHEN Xing-rui, JI Li, LI Shao-liang, QIAN Hai-hua, ZHANG Dan. Study on the Changes in Brain Function Characteristics of Chaihu Tongbian Decoction in Rats with Slow Transit Constipation and Depression Based on Resting-State Functional Magnetic Resonance Imaging[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(12): 1189-1197. doi: 10.14148/j.issn.1672-0482.2023.1189
shu

Study on the Changes in Brain Function Characteristics of Chaihu Tongbian Decoction in Rats with Slow Transit Constipation and Depression Based on Resting-State Functional Magnetic Resonance Imaging

doi: 10.14148/j.issn.1672-0482.2023.1189
  • 1.

    The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China

  • 2.

    Taihu Sanatorium of Jiangsu Province, Wuxi 214086, China

  • Received Date: 2023-08-23
    Available Online: 2023-12-20
    Abstract
  •   OBJECTIVE  To observe the changes in brain function characteristics of Chaihu Tongbian Decoction in rats with slow transit constipation (STC) and depression using resting-state functional magnetic resonance imaging (rs-fMRI).  METHODS  70 healthy SPF grade male SD rats were randomly divided into the blank group, model group, mosapride group, fluoxetine hydrochloride group, low (11.02 g·kg-1), medium (22.05 g·kg-1), and high (44.1 g·kg-1) dose Chaihu Tongbian Decoction groups. A rat model of STC with depression was constructed using compound diphenylephrine tablets combined with solitary confinement and chronic unpredictable mild stress (CUMS). Drugs were administered after successful modeling. The general condition of the rats was observed; 24 h total number of fecal particles, fecal water content and intestinal carbon end advance rate were calculated; HE staining of the colon tissue was performed; the depressive-like behavior of the rats was detected. By comparing rs-fMRI amplitude of low-frequency fluctuation (ALFF) values, the differences in neuronal activity in the brains of rats in each group were explored.  RESULTS  Compared with the blank group, the 24 h total number of fecal particles, fecal water content and intestinal carbon end advance rate in the model group were significantly reduced (P < 0.01). HE staining showed that the colon tissue lesions in the model group were severe. Open field experiment and sugar water consumption showed that the activity level of rats in the model group decreased (P < 0.01), and behavioral changes like anxiety and depression appeared. The rs-fMRI results showed that compared with the blank group, the ALFF value of the right posterolateral hippocampus in the model group decreased and the ALFF value of the left amygdala increased (P < 0.01). Compared with the model group, after 4 weeks of treatment, the 24 h total number of fecal particles, fecal water content, and intestinal carbon end advance rate in the mosapride group and each dose group of Chaihu Tongbian Decoction were improved (P < 0.05); compared with the model group, after 4 weeks of treatment, the fluoxetine hydrochloride group and each dose group of Chaihu Tongbian Decoction could improve the anxiety and depression-like behavior of rats (P < 0.05); compared with the model group, after 4 weeks of treatment, the fluoxetine hydrochloride group and the high-dose Chaihu Tongbian Decoction group could increase the ALFF value of the right posterolateral hippocampus and decrease the ALFF value of the left amygdala (P < 0.05).  CONCLUSION  Chaihu Tongbian Decoction can effectively relieve constipation and depressive symptoms in STC rats with depression, and its mechanism may be related to activating the right posterolateral hippocampus and inhibiting amygdala activity.

     

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    • References(38)
  • [1]
    李卫娜, 刘亚荣, 王春媚, 等. 中药配方颗粒制备通便膏方治疗慢性功能性便秘的疗效及对生活质量的影响[J]. 现代中西医结合杂志, 2019, 28(26): 2883-2888. doi: 10.3969/j.issn.1008-8849.2019.26.008

    LI WN, LIU YR, WANG CM, et al. Effect of Tongbian herbal paste prepared with formula granule on chronic functional constipation and its effect on quality of life[J]. Mod J Integr Tradit Chin West Med, 2019, 28(26): 2883-2888. doi: 10.3969/j.issn.1008-8849.2019.26.008
    [2]
    KAWAHARA H, OMURA N, AKIBA T. The usefulness of preoperative evaluation for intractable slow transit constipation by computed tomography[J]. J Anus Rectum Colon, 2021, 5(2): 144-147. doi: 10.23922/jarc.2020-065
    [3]
    郑舒泽, 郭强, 张贵民, 等. 首荟通便胶囊对慢传输型便秘模型小鼠的治疗作用及机制研究[J]. 中国中药杂志, 2021, 46(3): 520-525. doi: 10.19540/j.cnki.cjcmm.20201125.401

    ZHENG SZ, GUO Q, ZHANG GM, et al. Therapeutic effect and mechanism of Shouhui Tongbian Capsules on slow transit constipation model mice[J]. China J Chin Mater Med, 2021, 46(3): 520-525. doi: 10.19540/j.cnki.cjcmm.20201125.401
    [4]
    彭军良, 姚向阳, 陆金根, 等. 益气开秘方治疗气阴两虚型慢传输型便秘的临床研究[J]. 上海中医药杂志, 2018, 52(11): 50-53. https://www.cnki.com.cn/Article/CJFDTOTAL-SHZZ201811019.htm

    PENG JL, YAO XY, LU JG, et al. Clinical study on Yiqi Kaimi Formula in the treatment of slow transit constipation with syndrome of qi and Yin deficiency[J]. Shanghai J Tradit Chin Med, 2018, 52(11): 50-53. https://www.cnki.com.cn/Article/CJFDTOTAL-SHZZ201811019.htm
    [5]
    RAJINDRAJITH S, RANATHUNGA N, JAYAWICKRAMA N, et al. Behavioral and emotional problems in adolescents with constipation and their association with quality of life[J]. PLoS ONE, 2020, 15(10): e0239092. doi: 10.1371/journal.pone.0239092
    [6]
    BALLOU S, KATON J, SINGH P, et al. Chronic diarrhea and constipation are more common in depressed individuals[J]. Clin Gastroenterol Hepatol, 2019, 17(13): 2696-2703. doi: 10.1016/j.cgh.2019.03.046
    [7]
    HAN BJ. Correlation between gastrointestinal hormones and anxiety-depressive states in irritable bowel syndrome[J]. Exp Ther Med, 2013, 6(3): 715-720. doi: 10.3892/etm.2013.1211
    [8]
    张硕秋, 钱海华, 张丹. 从中医情志理论角度分析加味通便汤对慢性便秘的心理障碍疗效[J]. 辽宁中医杂志, 2021, 48(3): 62-65. doi: 10.13192/j.issn.1000-1719.2021.03.019

    ZHANG SQ, QIAN HH, ZHANG D. Analysis of effect of Jiawei Tongbian Decoction on chronic constipation from perspective of traditional Chinese medicine sentiment theory[J]. Liaoning J Tradit Chin Med, 2021, 48(3): 62-65. doi: 10.13192/j.issn.1000-1719.2021.03.019
    [9]
    周肸, 钱海华, 张丹, 等. 加用柴胡疏肝散治疗便秘患者心理障碍疗效观察[J]. 辽宁中医杂志, 2020, 47(5): 99-102. https://www.cnki.com.cn/Article/CJFDTOTAL-LNZY202005035.htm

    ZHOU X, QIAN HH, ZHANG D, et al. Clinical effect of Chaihu Shugan Decoction on patients with constipation[J]. Liaoning J Tradit Chin Med, 2020, 47(5): 99-102. https://www.cnki.com.cn/Article/CJFDTOTAL-LNZY202005035.htm
    [10]
    LIU L, HU CX, HU Y, et al. Abnormalities in the thalamo-cortical network in patients with functional constipation[J]. Brain Imaging Behav, 2021, 15(2): 630-642. doi: 10.1007/s11682-020-00273-y
    [11]
    刘峰林. 增液汤对慢传输型便秘小鼠水通道蛋白的实验研究[D]. 沈阳: 辽宁中医药大学, 2020.

    LIU FL. Experimental study of Zengye Decoction on aquaporin in mice with slow transit constipation[D]. Shenyang: Liaoning University of Traditional Chinese Medicine, 2020.
    [12]
    YANG H, LONG XY, YANG YH, et al. Amplitude of low frequency fluctuation within visual areas revealed by resting-state functional MRI[J]. NeuroImage, 2007, 36(1): 144-152. doi: 10.1016/j.neuroimage.2007.01.054
    [13]
    GAN Y, LIANG J, DIAO WJ, et al. Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite-induced constipation in Kunming mice[J]. Food Sci Nutr, 2020, 8(9): 5128-5137. doi: 10.1002/fsn3.1814
    [14]
    ZHANG XY, YANG HB, ZHENG JP, et al. Chitosan oligosaccharides attenuate loperamide-induced constipation through regulation of gut microbiota in mice[J]. Carbohydr Polym, 2021, 253: 117218. doi: 10.1016/j.carbpol.2020.117218
    [15]
    WANG LL, HU LJ, XU Q, et al. Bifidobacteria exert species-specific effects on constipation in BALB/c mice[J]. Food Funct, 2017, 8(10): 3587-3600. doi: 10.1039/C6FO01641C
    [16]
    LU YY, ZHANG JX, ZHANG Z, et al. Konjac glucomannan with probiotics acts as a combination laxative to relieve constipation in mice by increasing short-chain fatty acid metabolism and 5-hydroxytryptamine hormone release[J]. Nutrition, 2021, 84: 111112. doi: 10.1016/j.nut.2020.111112
    [17]
    SUN YH, YAN CQ, JIN SF, et al. Curative effect and mechanism of Guiren Runchang Granules on morphine-induced slow transit constipation in mice[J]. Evid Based Complement Alternat Med, 2020, 2020: 5493192.
    [18]
    吴旻娜, 王浩, 李悠然, 等. 枳术方对慢传输型便秘大鼠结肠5-HT4R及下游CaM-MLCK信号通路的影响[J]. 南京中医药大学学报, 2021, 37(6): 891-896. doi: 10.14148/j.issn.1672-0482.2021.0891

    WU MN, WANG H, LI YR, et al. Effect of Zhizhu Decoction on 5-HT4R and downstream CAM-MLCK signaling pathway in colon of rats with slow transit constipation[J]. J Nanjing Univ Tradit Chin Med, 2021, 37(6): 891-896. doi: 10.14148/j.issn.1672-0482.2021.0891
    [19]
    CHAO B, HUANG SJ, PAN JH, et al. Saikosaponin d downregulates microRNA-155 and upregulates FGF2 to improve depression-like behaviors in rats induced by unpredictable chronic mild stress by negatively regulating NF-κB[J]. Brain Res Bull, 2020, 157: 69-76. doi: 10.1016/j.brainresbull.2020.01.008
    [20]
    PIAO CH, SONG CH, LEE EJ, et al. Saikosaponin A ameliorates nasal inflammation by suppressing IL-6/ROR-γt/STAT3/IL-17/NF-κB pathway in OVA-induced allergic rhinitis[J]. Chem Biol Interact, 2020, 315: 108874. doi: 10.1016/j.cbi.2019.108874
    [21]
    GRUNDY D, AL-CHAER ED, AZIZ Q, et al. Fundamentals of neurogastroenterology: Basic science[J]. Gastroenterology, 2006, 130(5): 1391-1411.
    [22]
    CHENG C, CHAN AOO, HUI WM, et al. Coping strategies, illness perception, anxiety and depression of patients with idiopathic constipation: A population-based study[J]. Aliment Pharmacol Ther, 2003, 18(3): 319-326. doi: 10.1046/j.1365-2036.2003.01663.x
    [23]
    BOUCHOUCHA M, HEJNAR M, DEVROEDE G, et al. Patients with irritable bowel syndrome and constipation are more depressed than patients with functional constipation[J]. Dig Liver Dis, 2014, 46: 213-218.
    [24]
    LIU XJ, LIU HL, WEI FX, et al. Fecal metabolomics and network pharmacology reveal the correlations between constipation and depression[J]. J Proteome Res, 2021, 20(10): 4771-4786.
    [25]
    ZHU Q, CAI WW, ZHENG JY, et al. Distinct resting-state brain activity in patients with functional constipation[J]. Neurosci Lett, 2016, 632: 141-146.
    [26]
    ZHANG FF, PENG W, SWEENEY JA, et al. Brain structure alterations in depression: Psychoradiological evidence[J]. CNS Neurosci Ther, 2018, 24(11): 994-1003.
    [27]
    ZENG F, SUN RR, HE ZX, et al. Altered functional connectivity of the amygdala and sex differences in functional dyspepsia[J]. Clin Transl Gastroenterol, 2019, 10(6): e00046.
    [28]
    孙慧鑫, 何国富, 印为武, 等. 脑卒中后抑郁患者脑网络拓扑属性改变及其与临床症状的相关性[J]. 临床神经病学杂志, 2020, 33(5): 379-382. https://www.cnki.com.cn/Article/CJFDTOTAL-LCSJ202005020.htm

    SUN HX, HE GF, YIN WW, et al. Changes of brain network topological characteristics and their relationships with the clinical symptoms in patients with post-stroke depression[J]. J Clin Neurol, 2020, 33(5): 379-382. https://www.cnki.com.cn/Article/CJFDTOTAL-LCSJ202005020.htm
    [29]
    CHEN FH, POLSINELLI B, NAVA N, et al. Structural plasticity and molecular markers in hippocampus of male rats after acute stress[J]. Neuroscience, 2020, 438: 100-115.
    [30]
    李洋, 谢宇航, 王冉超, 等. 抑郁患者一级亲属的结构网络改变及其与抑郁发病的相关性研究[J]. 中华神经科杂志, 2022, 55(12): 1381-1388.

    LI Y, XIE YH, WANG RC, et al. Structural network changes in first-degree relatives of depressed patients and their correlation with the onset of depression[J]. Chin J Neurol, 2022, 55(12): 1381-1388.
    [31]
    ZHANG B, QI SL, LIU S, et al. Altered spontaneous neural activity in the precuneus, middle and superior frontal gyri, and hippocampus in college students with subclinical depression[J]. BMC Psychiatry, 2021, 21(1): 280.
    [32]
    DIAMOND MC, MURPHY GM Jr, AKIYAMA K, et al. Morphologic hippocampal asymmetry in male and female rats[J]. Exp Neurol, 1982, 76(3): 553-565.
    [33]
    RAǦBETLI MC, AYDINLIOǦLU A, KAPLAN S. Sex differences and right-left asymmetries in rat hippocampal components[J]. Int J Neurosci, 2002, 112(1): 81-95.
    [34]
    COLEMAN-MESCHES K, MCGAUGH JL. Differential involvement of the right and left amygdalae in expression of memory for aversively motivated training[J]. Brain Res, 1995, 670(1): 75-81.
    [35]
    ARTENI NS, PEREIRA LO, RODRIGUES AL, et al. Lateralized and sex-dependent behavioral and morphological effects of unilateral neonatal cerebral hypoxia-ischemia in the rat[J]. Behav Brain Res, 2010, 210(1): 92-98.
    [36]
    KIM S, SAPIURKA M, CLARK RE, et al. Contrasting effects on path integration after hippocampal damage in humans and rats[J]. Proc Natl Acad Sci USA, 2013, 110(12): 4732-4737.
    [37]
    QI ZG, WU X, WANG ZQ, et al. Impairment and compensation coexist in amnestic MCI default mode network[J]. NeuroImage, 2010, 50(1): 48-55.
    [38]
    孙继飞, 马跃, 郭春蕾, 等. 复发性与首发性抑郁症杏仁核静息态功能连接的对比研究[J]. 磁共振成像, 2022, 13(10): 144-149. https://www.cnki.com.cn/Article/CJFDTOTAL-CGZC202210022.htm

    SUN JF, MA Y, GUO CL, et al. Comparative study on resting functional connection of amygdala between recurrent and first-episode depression[J]. Chin J Magn Reson Imaging, 2022, 13(10): 144-149. https://www.cnki.com.cn/Article/CJFDTOTAL-CGZC202210022.htm
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