Molecular Identification of <i>Magnoliae Flos</i> and Its Relatives Based on DNA Barcoding

ZHANG Xin-yi; QIU Yu-xuan; HU Yang; TIAN Rong; ZHAO Mian; YAN Hui; WANG Xia-chang; HU Li-hong

doi:10.14148/j.issn.1672-0482.2023.1086

Volume 39 Issue 11

Nov. 2023

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Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2023 > 39(11): 1086-1094

ZHANG Xin-yi, QIU Yu-xuan, HU Yang, TIAN Rong, ZHAO Mian, YAN Hui, WANG Xia-chang, HU Li-hong. Molecular Identification of Magnoliae Flos and Its Relatives Based on DNA Barcoding[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(11): 1086-1094. doi: 10.14148/j.issn.1672-0482.2023.1086

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ZHANG Xin-yi, QIU Yu-xuan, HU Yang, TIAN Rong, ZHAO Mian, YAN Hui, WANG Xia-chang, HU Li-hong. Molecular Identification of Magnoliae Flos and Its Relatives Based on DNA Barcoding[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(11): 1086-1094. doi: 10.14148/j.issn.1672-0482.2023.1086

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Molecular Identification of Magnoliae Flos and Its Relatives Based on DNA Barcoding

doi: 10.14148/j.issn.1672-0482.2023.1086

ZHANG Xin-yi^{1, 2
,},
QIU Yu-xuan^{1, 2},
HU Yang^{1, 2
,
,},
TIAN Rong²,
ZHAO Mian²,
YAN Hui²,
WANG Xia-chang^{1, 2},
HU Li-hong^{1, 2
,
,}

1.
Jiangsu Key Laboratory for Functional Substances of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

Received Date: 2023-04-11
Available Online: 2023-11-24

Abstract

Abstract

OBJECTIVE To identify Magnoliae Flos and its related species using DNA barcoding technology, so as to establish a rapid, accurate and convenient method for identification of Magnoliae Flos. METHODS Genomic DNA of 21 Magnoliae Flos and their related species were extracted, and ITS, ITS2, psbA-trnH, matK and rbcL gene sequences were amplified and sequenced. MEGA7.0 software was used for sequence comparison, and the intraspecific and interspecific genetic distance was calculated based on Kimura-2-Parameter (K2P) model to evaluate Barcoding gap, and the NJ phylogenetic trees were constructed to react identification results. RESULTS Among the 5 primers, psbA-trnH and matK could normally amplify the target gene, and the success rate of them was higher than 98%, while the success rate of rbcL sequencing was 38.1% only. The matK sequence had the highest number of variation sites, and psbA-trnH had the most prominent species discriminatory on Barcoding gap analysis relative to matK and rbcL. The NJ phylogenetic tree showed that psbA-trnH+matK barcode sequence could accurately identify 9 varieties, and distinguish Magnoliae Flos and its relatives. In addition, we applied it to the molecular identification of Magnolia grafting rootstocks for the first time. CONCLUSION The sequence combination of psbA-trnH+matK can realize the accurate identification of Magnoliae Flos, and can be applied to the rapid identification of Magnolia grafting rootstocks.
- Magnoliae Flos,
- DNA barcoding,
- molecular identification,
- psbA-trnH+matK,
- grafting reproduction

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References(26)

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Molecular Identification of Magnoliae Flos and Its Relatives Based on DNA Barcoding

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