Effect Evaluation and Mechanism of Jujing Pills in Improving Retinal Injury in Subacute Aging Mice Induced by D-galactose

LU Yun-qing; CAO Cheng; ZHENG Jia-ni; ZHU Zi-qiang; TONG Jia-xiang; QI Cheng-cheng; YUAN Lei; GUO Sheng; SU Shu-lan; KANG Hong-jie; DUAN Jin-ao; ZHU Yue

doi:10.14148/j.issn.1672-0482.2023.0849

Volume 39 Issue 9

Sep. 2023

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Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2023 > 39(9): 849-859

LU Yun-qing, CAO Cheng, ZHENG Jia-ni, ZHU Zi-qiang, TONG Jia-xiang, QI Cheng-cheng, YUAN Lei, GUO Sheng, SU Shu-lan, KANG Hong-jie, DUAN Jin-ao, ZHU Yue. Effect Evaluation and Mechanism of Jujing Pills in Improving Retinal Injury in Subacute Aging Mice Induced by D-galactose[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(9): 849-859. doi: 10.14148/j.issn.1672-0482.2023.0849

Citation:

LU Yun-qing, CAO Cheng, ZHENG Jia-ni, ZHU Zi-qiang, TONG Jia-xiang, QI Cheng-cheng, YUAN Lei, GUO Sheng, SU Shu-lan, KANG Hong-jie, DUAN Jin-ao, ZHU Yue. Effect Evaluation and Mechanism of Jujing Pills in Improving Retinal Injury in Subacute Aging Mice Induced by D-galactose[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(9): 849-859. doi: 10.14148/j.issn.1672-0482.2023.0849

Citation:

LU Yun-qing, CAO Cheng, ZHENG Jia-ni, ZHU Zi-qiang, TONG Jia-xiang, QI Cheng-cheng, YUAN Lei, GUO Sheng, SU Shu-lan, KANG Hong-jie, DUAN Jin-ao, ZHU Yue. Effect Evaluation and Mechanism of Jujing Pills in Improving Retinal Injury in Subacute Aging Mice Induced by D-galactose[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(9): 849-859. doi: 10.14148/j.issn.1672-0482.2023.0849

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Effect Evaluation and Mechanism of Jujing Pills in Improving Retinal Injury in Subacute Aging Mice Induced by D-galactose

doi: 10.14148/j.issn.1672-0482.2023.0849

1.
School of Pharmacy, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Province Key Laboratory of High Technology Research, Nanjing University of Chinese Medicine, Nanjing 210023, China
2.
Ningxia Innovation Center of Goji, Yinchuan 750002, China

Received Date: 2023-07-15
Available Online: 2023-10-20

Abstract

Abstract

OBJECTIVE To evaluate the effect of Jujing Pills on retinal injury in subacute aging mice induced by D-galactose and explore its mechanism. METHODS A subacute aging model was constructed by subcutaneous injection of D-galactose into mice. Western medicine positive drug AREDS2, Chinese medicine positive drug Qiju Dihuang Pills, low and high dose groups of water extract of Jujing Pill, and low and high dose groups of 50% ethanol extract of Jujing Pill were administered, respectively. Optical coherence tomography (OCT) was used to evaluate the condition of mouse retina and fundus, HE staining was adopted to assess retinal cell damage, TUNEL staining was applied to observe retinal cell apoptosis, kits were used to measure the expression of oxidative stress factors, and western blotting was used to detect the expression of retinal apoptosis and oxidative stress pathway related proteins in mice. RESULTS The extracts of Jujing Pills significantly increased the retinal thickness (P < 0.05), improved the retinal injury and inhibited the apoptosis of retinal cells in model mice (P < 0.05). At the same time, Jujing Pills down-regulated the expression of apoptotic proteins such as Cleaved Caspase-3 and Cleaved Caspase-9 (P < 0.05) while up-regulated the expression ratio of Bcl-2/Bax (P < 0.05). Jujing Pills also significantly increased the expression of antioxidant factors SOD and GSH (P < 0.05), decreased the production of oxidative product MDA (P < 0.05), and significantly up-regulated the expression of antioxidant proteins Nrf2 and HO-1(P < 0.05). The water extracts of Jujing Pills exerted similar retina protection effect with the 50% ethanol extracts. CONCLUSION Jujing Pills can improve the retinal injury of subacute aging model mice, which is related to the inhibition of retinal cell apoptosis and the improvement of oxidative stress.
- Jujing Pills,
- subacute aging,
- retinal injury,
- oxidative stress,
- apoptosis

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References(29)

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