Volume 39 Issue 7
Jul.  2023
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2023  >  39(7): 657-668
HUANG Yao, CHEN Qiao-hua, FANG Chao-zuan, DENG Yi-fang, WANG Wen-li, HU Yi, ZHANG Zhi-peng. Analysis of Changes of Sophorae Flos before and after Processing Based on UPLC Fingerprint and Chromatic Value[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 657-668. doi: 10.14148/j.issn.1672-0482.2023.0657
Citation: HUANG Yao, CHEN Qiao-hua, FANG Chao-zuan, DENG Yi-fang, WANG Wen-li, HU Yi, ZHANG Zhi-peng. Analysis of Changes of Sophorae Flos before and after Processing Based on UPLC Fingerprint and Chromatic Value[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 657-668. doi: 10.14148/j.issn.1672-0482.2023.0657
shu

Analysis of Changes of Sophorae Flos before and after Processing Based on UPLC Fingerprint and Chromatic Value

doi: 10.14148/j.issn.1672-0482.2023.0657

  • Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Guangdong Yifang Pharmaceutical Co.Ltd., Foshan 528244, China

  • Received Date: 2023-02-06
    Available Online: 2023-07-15
    Abstract
  •   OBJECTIVE  To analyze the difference and dynamic change rules of UPLC fingerprint and chromatic value of Sophorae Flos before and after processing, and to provide reference for the identification and processing technology of Sophorae Flos, fried Sophorae Flos, and Sophorae Flos carbonisatus.  METHODS  The UPLC fingerprint of Sophorae Flos before and after processing was established, and chromatic value was determined by spectrophotometer.The differences of Sophorae Flos, fried Sophorae Flos, and Sophorae Flos carbonisatus were analyzed according to the similarity evaluation, one-way ANOVA, cluster analysis, principal component analysis, orthogonal partial least squares discriminant analysis (OPLS-DA), et al.The dynamic change rule of the fingerprint and chromatic value of Sophorae Flos at different processing time points were also analyzed.  RESULTS  There were obvious differences in fingerprints and chromatic value before and after processing.15 common peaks were calibrated for Sophorae Flos, 16 for fried Sophorae Flos, and 14 for Sophorae Flos carbonisatus.Peak 2, 3, 8, 9, 10, 11, 14, and 15 were identified as 5-HMF, protocatechuic acid, rutin, isoquercetin, kaempferol-3-O-rutinoside, narcissin, quercetin, and kaempferol, respectively.Peak 1 and peak 2(5-HMF) were the chemical components produced after processing and peak 17 was the component disappeared after processing.There was a significant change in the chromaticity value before and after the processing of Sophorae Flos.The chromaticity values L*, b*, E* of Sophorae Flos carbonisatus were much lower than those of Sophorae Flos and fried Sophorae Flos.The chromaticity value a* of fried Sophorae Flos was slightly higher than that of Sophorae Flos and Sophorae Flos carbonisatus.It was possible to distinguish samples of Sophorae Flos, fried Sophorae Flos, and Sophorae Flos carbonisatus by the chromaticity value.Cluster analysis and principal component analysis could distinguish Sophorae Flos, fried Sophorae Flos, and Sophorae Flos carbonisatus.The results of orthogonal partial least squares discriminant analysis show that rutin and quercetin were the important components causing the difference between Sophorae Flos and its processed products (VIP>1).Within 46 min of processing of Sophorae Flos, the ratio of peak area/scale sample size/1 000 of peak 1 to peak 16 increased first and then decreased, while peak 17 decreased continuously.Using the color of the slices as an evaluation indicator when the frying temperature was 160℃, the appropriate frying time for fried Sophorae Flos, Sophorae Flos carbonisatus should be 4-6 min and 14-18 min, respectively.  CONCLUSION  The established UPLC fingerprint and chromatic value determination method can provide reference for the identification and processing technology of Sophorae Flos, fried Sophorae Flos, and Sophorae Flos carbonisatus.

     

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    • References(13)
  • [1]
    国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 370.

    Chinese Pharmacopoeia Commission. Pharmacopoeia of The People ' s Republic of China: Ⅰ[M]. Beijing: China medical science press, 2020: 370.
    [2]
    王笑, 王雨, 张冰, 等. 槐不同药用部位本草学、化学成分和药理作用研究进展[J]. 中草药, 2018, 49(18): 4461-4467. doi: 10.7501/j.issn.0253-2670.2018.18.035

    WANG X, WANG Y, ZHANG B, et al. Research progress on herbaceous, chemical constituents and pharmacological effects of different medicinal parts of Sophora japonica[J]. Chin Tradit Herb Drugs, 2018, 49(18): 4461-4467. doi: 10.7501/j.issn.0253-2670.2018.18.035
    [3]
    马艳琴, 郭珊珊, 马进, 等. 不同槐花炮制品中总黄酮含量的比较研究[J]. 时珍国医国药, 2018, 29(1): 76-78. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY201801025.htm

    MA YQ, GUO SS, MA J, et al. Comparative study on total flavonoids content in different processed products of Sophora japonica[J]. Lishizhen Med Mater Med Res, 2018, 29(1): 76-78. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY201801025.htm
    [4]
    朱洪剑, 李鑫. 不同炮制方法对槐花有效成分百分含量的影响[J]. 哈尔滨商业大学学报(自然科学版), 2018, 34(4): 392-397, 401. https://www.cnki.com.cn/Article/CJFDTOTAL-HLJS201804004.htm

    ZHU HJ, LI X. Influence of different processing methods on component content of Sophora japonica[J]. J Harbin Univ Commer Nat Sci Ed, 2018, 34(4): 392-397, 401. https://www.cnki.com.cn/Article/CJFDTOTAL-HLJS201804004.htm
    [5]
    钟文峰, 洪婉敏, 刘晓霞, 等. 基于特征图谱结合效毒值与外观性状的蜜马兜铃炮制工艺研究[J]. 南京中医药大学学报, 2023, 39(2): 157-169. doi: 10.14148/j.issn.1672-0482.2023.0157

    ZHONG WF, HONG WM, LIU XX, et al. Study on processing technology of honey Aristolochia based on characteristic map combined with toxicity value and appearance characteristics[J]. J Nanjing Univ Tradit Chin Med, 2023, 39(2): 157-169. doi: 10.14148/j.issn.1672-0482.2023.0157
    [6]
    邓李红, 王寿富, 陈仕妍, 等. 基于UPLC特征图谱结合多模式识别和色度值的槐角炮制前后差异研究[J]. 中草药, 2022, 53(21): 6881-6887. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202221027.htm

    DENG LH, WANG SF, CHEN SY, et al. Comparative study on UPLC characteristic chromatogram combined with multiple chemical pattern recognition and chromatic value of Sophorae Fructus and its processed products[J]. Chin Tradit Herb Drugs, 2022, 53(21): 6881-6887. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202221027.htm
    [7]
    姚令文, 刘燕, 郑笑为, 等. 指纹图谱、特征图谱技术在中药材和中成药中的应用[J]. 中国新药杂志, 2018, 27(8): 934-939. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXYZ201808016.htm

    YAO LW, LIU Y, ZHENG XW, et al. Application of the fingerprint and characteristic fingerprint technology in traditional Chinese medicinal materials and Chinese patent medicine[J]. Chin J New Drugs, 2018, 27(8): 934-939. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXYZ201808016.htm
    [8]
    杨丽, 龚燚婷, 许铭珊, 等. 基于"表里关联"的大黄炭炮制过程颜色和成分变化关系研究[J]. 中草药, 2020, 51(22): 5705-5713. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202022006.htm

    YANG L, GONG YT, XU MS, et al. Research on relationship between color and composition changes in processing of rhubarb charcoal based on "external and internal correlation"[J]. Chin Tradit Herb Drugs, 2020, 51(22): 5705-5713. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202022006.htm
    [9]
    王梅, 王越欣, 武英茹, 等. 5-羟甲基糠醛在中药加工过程中的变化及药理作用研究概况[J]. 药物评价研究, 2020, 43(11): 2319-2327. https://www.cnki.com.cn/Article/CJFDTOTAL-YWPJ202011030.htm

    WANG M, WANG YX, WU YR, et al. Research progress on content changes of 5-hydroxymethyl furfural during processing of Chinese medicine and it ' s pharmacological effects[J]. Drug Eval Res, 2020, 43(11): 2319-2327. https://www.cnki.com.cn/Article/CJFDTOTAL-YWPJ202011030.htm
    [10]
    黄梦婷, 潘玲, 邓李红, 等. 基于UPLC指纹图谱与色度值的桑白皮生品和炮制品的鉴别及炮制终点研究[J]. 中国药房, 2021, 32(1): 56-63. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA202101011.htm

    HUANG MT, PAN L, DENG LH, et al. Identification and study on processing end-point of raw and processed products of Morus alba based on UPLC fingerprint and chromaticity[J]. China Pharm, 2021, 32(1): 56-63. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA202101011.htm
    [11]
    崔婷, 方朝缵, 田甜, 等. 基于HPLC指纹图谱与多成分含量测定的骨碎补不同炮制品差异性研究[J]. 中药材, 2021, 44(3): 580-585. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA202103013.htm

    CUI T, FANG CZ, TIAN T, et al. Study on the differences of different processed products of drynariae rhizoma based on HPLC fingerprint and multi-components content determination[J]. J Chin Med Mater, 2021, 44(3): 580-585. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA202103013.htm
    [12]
    张雪, 李晓庆, 王云, 等. 焦栀子炒制过程中HPLC图谱变化与外观颜色的动态关联研究[J]. 中草药, 2018, 49(17): 4029-4037. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201817010.htm

    ZHANG X, LI XQ, WANG Y, et al. Dynamic correlation between constituents detected by HPLC and colors of samples in stir-frying process of Gardeniae Fructus Praeparatus[J]. Chin Tradit Herb Drugs, 2018, 49(17): 4029-4037. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201817010.htm
    [13]
    董喜恩, 蒋锋, 罗根祥. 糖类脱水生成5-羟甲基糠醛的研究进展[J]. 化工中间体, 2010, 6(2): 17-22. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJTY201002014.htm

    DONG XE, JIANG F, LUO GX. Dehydration of Carbohydrates into 5-Hydroxymethylfurfural in the presence of double micro-porous zeolite catalyst[J]. Chem Intermed, 2010, 6(2): 17-22. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJTY201002014.htm
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