Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4<sup>+</sup>T, CD8<sup>+</sup>T and NK Cells

MIN Yi; ZHANG Xi-yao; XIANG Dong-yang; CHEN Jing; LYU Zhi-yang; SHAN Xin

doi:10.14148/j.issn.1672-0482.2023.0637

Volume 39 Issue 7

Jul. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2023 > 39(7): 637-644

MIN Yi, ZHANG Xi-yao, XIANG Dong-yang, CHEN Jing, LYU Zhi-yang, SHAN Xin. Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4+T, CD8+T and NK Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 637-644. doi: 10.14148/j.issn.1672-0482.2023.0637

Citation:

MIN Yi, ZHANG Xi-yao, XIANG Dong-yang, CHEN Jing, LYU Zhi-yang, SHAN Xin. Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4⁺T, CD8⁺T and NK Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 637-644. doi: 10.14148/j.issn.1672-0482.2023.0637

Citation:

MIN Yi, ZHANG Xi-yao, XIANG Dong-yang, CHEN Jing, LYU Zhi-yang, SHAN Xin. Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4⁺T, CD8⁺T and NK Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 637-644. doi: 10.14148/j.issn.1672-0482.2023.0637

PDF( 3914 KB)

Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4⁺T, CD8⁺T and NK Cells

doi: 10.14148/j.issn.1672-0482.2023.0637

MIN Yi^{1, 2
,},
ZHANG Xi-yao^{1, 2},
XIANG Dong-yang¹,
CHEN Jing¹,
LYU Zhi-yang¹,
SHAN Xin^{1
,
,}

1.
Hanlin College, Nanjing University of Chinese Medicine, Taizhou 225300, China
2.
Lung Disease Department, Yixing Hospital of Traditional Chinese Medicine, Wuxi 214299, China

Received Date: 2022-11-10
Available Online: 2023-07-15

Abstract

Abstract

OBJECTIVE Aimed to examine the effect of Euryale ferox petioles and pedicels polysaccharide (EFPP) on LLC cell allograft model of lung cancer. METHODS Forty male C57BL/6 rats were randomly divided into 4 groups (n=10/group), namely control group, EFPP low dose group (100 mg·kg^-1 EFPP), EFPP high dose group (200 mg·kg^-1 EFPP), 5-Fu(20 mg·kg^-15-Fu). Different doses of EFPP were orally administered once daily for fifteen consecutive days, and the positive drug was injected once every 3 days after the ectopic xenograft tumor model was established. Rats in control group received PBS treatment by gavage. All mice were sacrificed on the second day of the last administration. Tumor samples were taken from each group, and the volume and tumor weight were weighed. Hematoxylin and eosin staining, flow cytometry, TUNEL apoptosis staining and Western blot were used to detect the pharmacodynamics of EFPP. RESULTS The data showed that EFPP could inhibit the proliferation of LLC cells in vivo. In addition, EFPP increased the levels of CD3⁺CD4⁺T, CD3⁺CD8⁺T and CD3^-NK1.1⁺cells. At the same time, EFPP up-regulated the expression of proteins related to the Caspase3 apoptosis pathway. CONCLUSION EFPP can promote LLC cell apoptosis by increasing the infiltration of lymphocyte subsets, up-regulating the expression of cleaved-caspase3 and Bax, and down-regulating the expression of Bcl2. This study provided the scientific basis for the anti-tumor application of EFPP and utilization of euryale ferox stem resources.
- Euryale ferox petioles and pedicels polysaccharide,
- CD4⁺T cells,
- CD8⁺T cells,
- NK cells,
- Caspase3,
- apoptotic pathway

FullText(HTML)

References(14)

References

[1]	XIA CF, DONG XS, LI H, et al. Cancer statistics in China and United States, 2022: Profiles, trends, and determinants[J]. Chin Med J, 2022, 135(5): 584-590. doi: 10.1097/CM9.0000000000002108
[2]	WU DW, HUANG HY, TANG Y, et al. Progress on clinical trials of cancer drugs in China, 2020[J]. Chin J Oncol, 2021, 43(2): 218-223.
[3]	FERRIS RL, JAFFEE EM, FERRONE S. Tumor antigen-targeted, monoclonal antibody-based immunotherapy: Clinical response, cellular immunity, and immunoescape[J]. J Clin Oncol, 2010, 28(28): 4390-4399. doi: 10.1200/JCO.2009.27.6360
[4]	XIA Y, LI W, LI Y, et al. The clinical value of the changes of peripheral lymphocyte subsets absolute counts in patients with non-small cell lung cancer[J]. Transl Oncol, 2020, 13(12): 100849. doi: 10.1016/j.tranon.2020.100849
[5]	ZHUO Y, LI SJ, HU W, et al. Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathway[J]. J Immunother Cancer, 2022, 10(5): e004113. doi: 10.1136/jitc-2021-004113
[6]	LIU PC, FENG XW, ZHAO XM, et al. Abnormal expression and clinical significance of surface receptors on natural killer cells in the peripheral blood of patients with non-small cell lung cancer[J]. Neoplasma, 2022, 69(4): 931-939. doi: 10.4149/neo_2022_220219N188
[7]	王倩. 芡非药用部位(茎)资源开发利用研究[D]. 南京: 南京中医药大学, 2019. WANG Q. Study on the development and utilization of Euryale ferox non-medicinal parts (petioles and pedicels) resources[D]. Nanjing: Nanjing University of Chinese Medicine, 2019.
[8]	SARKAR C, CHAKROBORTY D, DASGUPTA PS, et al. Dopamine is a safe antiangiogenic drug which can also prevent 5-fluorouracil induced neutropenia[J]. Int J Cancer, 2015, 137(3): 744-749. doi: 10.1002/ijc.29414
[9]	WANG XS, WANG D. Clinical analysis of 125I seed implantation combined with epidermal growth factor receptor-tyrosine kinase inhibitors in advanced non-small cell lung cancer[J]. J BUON, 2021, 26(5): 1879-1886.
[10]	WANG ZX, ZHU J, LIU YF, et al. Tumor-polarized GPX3⁺ AT2 lung epithelial cells promote premetastatic niche formation[J]. Proc Natl Acad Sci USA, 2022, 119(32): e2201899119. doi: 10.1073/pnas.2201899119
[11]	LIU M, WANG KX, JI WX, et al. FGFR1 promotes tumor immune evasion via YAP-mediated PD-L1 expression upregulation in lung squamous cell carcinoma[J]. Cell Immunol, 2022, 379: 104577. doi: 10.1016/j.cellimm.2022.104577
[12]	YANG H, JIA H, ZHAO Q, et al. Visualization of natural killer cell-mediated killing of cancer cells at single-cell resolution in live zebrafish[J]. Biosens Bioelectron, 2022, 216: 114616. doi: 10.1016/j.bios.2022.114616
[13]	ZHENG QW, NI QZ, ZHU B, et al. PPDPF promotes lung adenocarcinoma progression via inhibiting apoptosis and NK cell-mediated cytotoxicity through STAT3[J]. Oncogene, 2022, 41(36): 4244-4256. doi: 10.1038/s41388-022-02418-3
[14]	CHIAWPANIT C, PANWONG S, SAWASDEE N, et al. Genistein sensitizes human cholangiocarcinoma cell lines to be susceptible to natural killer cells[J]. Biology, 2022, 11(8): 1098. doi: 10.3390/biology11081098

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(6)

Get Citation

PDF

XML

Article Metrics

Article views (185) PDF downloads(7)

Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4⁺T, CD8⁺T and NK Cells

doi: 10.14148/j.issn.1672-0482.2023.0637

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4+T, CD8+T and NK Cells

doi: 10.14148/j.issn.1672-0482.2023.0637

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content

Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4⁺T, CD8⁺T and NK Cells