Volume 39 Issue 7
Jul.  2023
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2023  >  39(7): 618-628
CHEN Jia-bin, ZHANG Meng-yao, WANG Dan, HUANG Tong-xing, SHAN Jin-jun, YANG Jun-chao, SONG Kang, LIN Li-li. Study on Lipid Metabolism Regulated by Polygonum Cuspidatum in the Treatment of Pulmonary Fibrosis Model of Respiratory Syncytial Virus Infection in Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 618-628. doi: 10.14148/j.issn.1672-0482.2023.0618
Citation: CHEN Jia-bin, ZHANG Meng-yao, WANG Dan, HUANG Tong-xing, SHAN Jin-jun, YANG Jun-chao, SONG Kang, LIN Li-li. Study on Lipid Metabolism Regulated by Polygonum Cuspidatum in the Treatment of Pulmonary Fibrosis Model of Respiratory Syncytial Virus Infection in Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(7): 618-628. doi: 10.14148/j.issn.1672-0482.2023.0618
shu

Study on Lipid Metabolism Regulated by Polygonum Cuspidatum in the Treatment of Pulmonary Fibrosis Model of Respiratory Syncytial Virus Infection in Mice

doi: 10.14148/j.issn.1672-0482.2023.0618
  • 1.

    The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China

  • 2.

    Institute of Pediatrics, Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing 210023, China

  • Received Date: 2022-12-07
    Available Online: 2023-07-15
    Abstract
  •   OBJECTIVE  To investigate the effects of Polygonum Cuspidatum in the treatment of lung fibrosis mice infected with the respiratory syncytial virus (RSV) in terms of altered lipids and the inflammatory process in the lung.  METHODS  C57BL/6 mice were given a tracheal drip of 50 μL RSV venom (4×106 PFU·mL-1) combined with bleomycin (2 mg·mL-1). The mice were randomly divided into blank group, bleomycin induced pulmonary fibrosis group, RSV infected pulmonary fibrosis group and Polygonum Cuspidatum (4.55 g·kg-1·d-1) treatment group. To assess changes in pathological and pro-inflammatory cytokine levels (IL-6, and TNF-α), lung tissues from each group were removed 21 days after modeling. Lipidomics analysis was then completed using UPLC-Q Exactive Orbitrap/MS.  RESULTS  In comparison to the blank group, the lungs of the bleomycin induced pulmonary fibrosis group and RSV infected pulmonary fibrosis group showed prominent collagen fiber deposition and inflammatory infiltration, and the levels of lung inflammatory factors like IL-6 and TNF-α were noticeably higher (P < 0.05). The lipid metabolism of the RSV infected pulmonary fibrosis group differed significantly from that of the bleomycin induced pulmonary fibrosis group, with abnormal levels of ceramide (Cer), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), acylethanolamine (NAE), sphingomyelin (SM), diglyceride (DG), triglyceride (TG) and fatty acid (FA). The above indexes showed a significant trend of correction after the intervention of Polygonum Cuspidatum (4.55 g·kg-1·d-1).  CONCLUSION  RSV has the potential to accelerate the progression of pulmonary fibrosis. Polygonum Cuspidatum reduces inflammation and collagen deposition in the lung tissue of RSV-infected mice with pulmonary fibrosis by regulating lipid metabolism, and it slows the progression of pulmonary fibrosis in mice.

     

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