Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

LI Xin-ru; SHENG Xian-jie; YANG Yan; AN Zhen-tao; KANG An; LI Hui; GE Fei

doi:10.14148/j.issn.1672-0482.2023.0442

Volume 39 Issue 5

May 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2023 > 39(5): 442-451

LI Xin-ru, SHENG Xian-jie, YANG Yan, AN Zhen-tao, KANG An, LI Hui, GE Fei. Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(5): 442-451. doi: 10.14148/j.issn.1672-0482.2023.0442

Citation:

LI Xin-ru, SHENG Xian-jie, YANG Yan, AN Zhen-tao, KANG An, LI Hui, GE Fei. Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(5): 442-451. doi: 10.14148/j.issn.1672-0482.2023.0442

Citation:

LI Xin-ru, SHENG Xian-jie, YANG Yan, AN Zhen-tao, KANG An, LI Hui, GE Fei. Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(5): 442-451. doi: 10.14148/j.issn.1672-0482.2023.0442

PDF( 4307 KB)

Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

doi: 10.14148/j.issn.1672-0482.2023.0442

LI Xin-ru^{1, 2
,},
SHENG Xian-jie^{1, 2},
YANG Yan¹,
AN Zhen-tao³,
KANG An^{1, 2},
LI Hui^{3
,
,},
GE Fei^{1
,
,}

1.
Hai'an Hospital of Traditional Chinese Medicine Affiliated to Hanlin College of Nanjing University of Chinese Medicine, Nantong 226600, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
3.
Integrated Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangsu Academy of Traditional Chinese Medicine, Nanjing 210028, China

Received Date: 2023-01-06
Available Online: 2023-05-19

Abstract

Abstract

OBJECTIVE To explore the effects of four kinds of heat-clearing and dampness-drying herbs on intestinal flora, bile acids and short chain fatty acids (SCFAs) in normal mice at long-term clinical equivalent doses. METHODS Thirty Balb/c male mice were randomly divided into control group, Sophora flavescens Ait. (KS) group, Phellodendron chinense Schneid. (HB) group, Coptis chinensis Franch. (HL) group and Scutellaria baicalensis Georgi (HQ) group, with 6 mice in each group. Except for the control group, KS group, HB group, HL group and HQ group were orally administrated at a dosage of 2.34 g·kg^-1, 3.12 g·kg^-1, 1.3 g·kg^-1 and 2.6 g·kg^-1, respectively, for two weeks. The contents of short chain fatty acids and bile acids in feces were detected by liquid chromatography tandem mass spectrometry, and the structural changes of microflora in intestinal contents were detected by 16S rRNA high-throughput gene sequencing technology. RESULTS There was no significant effect of heat-clearing and dampness-drying Chinese medicine on the contents of six short-chain fatty acids in the feces of normal mice. Among the 23 common bile acids, when compared with the control group, the numbers of the significantly changed bile acids in HQ group, KS group, HL group and HB group were 4, 3, 2 and 1, respectively. From the perspective of changes in the abundance of intestinal flora, compared with the control group, except for the KS group, Firmicutes in the HL group, HQ group and HB group all showed a downward trend, and the Bacteroides showed an upward trend, among which the changes in the HL group were the most significant. CONCLUSION Sophora flavescens Ait., Phellodendron chinense Schneid., Coptis chinensis Franch. and Scutellaria baicalensis Georgi have little effect on SCFAs in normal mice, but can affect bile acid content by changing the structure of intestinal flora.
- clearing heat and drying dampness,
- short-chain fatty acids,
- bile acids,
- intestinal flora

FullText(HTML)

References(32)

References

[1]	ADAK A, KHAN MR. An insight into gut microbiota and its functionalities[J]. Cell Mol Life Sci, 2019, 76(3): 473-493. doi: 10.1007/s00018-018-2943-4
[2]	SINGH RK, CHANG HW, YAN D, et al. Influence of diet on the gut microbiome and implications for human health[J]. J Transl Med, 2017, 15(1): 73. doi: 10.1186/s12967-017-1175-y
[3]	SCHOELER M, CAESAR R. Dietary lipids, gut microbiota and lipid metabolism[J]. Rev Endocr Metab Disord, 2019, 20(4): 461-472. doi: 10.1007/s11154-019-09512-0
[4]	牛璐, 王跃飞, 赵鑫, 等. 中药调控肠道菌群代谢产物的研究进展[J]. 天津中医药, 2021, 38(2): 254-260. https://www.cnki.com.cn/Article/CJFDTOTAL-TJZY202102029.htm NIU L, WANG YF, ZHAO X, et al. Research progress on the regulation of gut microbial metabolites by traditional Chinese medicine[J]. J Tianjin Univ Tradit Chin Med, 2021, 38(2): 254-260. https://www.cnki.com.cn/Article/CJFDTOTAL-TJZY202102029.htm
[5]	RATAJCZAK W, RYŁ A, MIZERSKI A, et al. Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs)[J]. Acta Biochim Pol, 2019, 66(1): 1-12.
[6]	YANG WJ, YU TM, HUANG XS, et al. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity[J]. Nat Commun, 2020, 11(1): 4457. doi: 10.1038/s41467-020-18262-6
[7]	WANG G, YU Y, WANG YZ, et al. Role of SCFAs in gut microbiome and glycolysis for colorectal cancer therapy[J]. J Cell Physiol, 2019, 234(10): 17023-17049. doi: 10.1002/jcp.28436
[8]	PORTINCASA P, BONFRATE L, VACCA M, et al. Gut microbiota and short chain fatty acids: Implications in glucose homeostasis[J]. Int J Mol Sci, 2022, 23(3): 1105. doi: 10.3390/ijms23031105
[9]	HUANG W, MAN Y, GAO CL, et al. Short-chain fatty acids ameliorate diabetic nephropathy via GPR43-mediated inhibition of oxidative stress and NF-κB signaling[J]. Oxid Med Cell Longev, 2020, 2020: 4074832.
[10]	COLOSIMO S, TOMLINSON JW. Bile acids as drivers and biomarkers of hepatocellular carcinoma[J]. World J Hepatol, 2022, 14(9): 1730-1738. doi: 10.4254/wjh.v14.i9.1730
[11]	LI JN, DAWSON PA. Animal models to study bile acid metabolism[J]. Biochim Biophys Acta Mol Basis Dis, 2019, 1865(5): 895-911. doi: 10.1016/j.bbadis.2018.05.011
[12]	WINSTON JA, THERIOT CM. Diversification of host bile acids by members of the gut microbiota[J]. Gut Microbes, 2020, 11(2): 158-171. doi: 10.1080/19490976.2019.1674124
[13]	MCKENZIE C, TAN J, MACIA L, et al. The nutrition-gut microbiome-physiology axis and allergic diseases[J]. Immunol Rev, 2017, 278(1): 277-295. doi: 10.1111/imr.12556
[14]	FIORUCCI S, CARINO A, BALDONI M, et al. Bile acid signaling in inflammatory bowel diseases[J]. Dig Dis Sci, 2021, 66(3): 674-693. doi: 10.1007/s10620-020-06715-3
[15]	DELEU S, MACHIELS K, RAES J, et al. Short chain fatty acids and its producing organisms: An overlooked therapy for IBD?[J]. EBioMedicine, 2021, 66: 103293. doi: 10.1016/j.ebiom.2021.103293
[16]	WANG J, WANG L, LOU GH, et al. Coptidis Rhizoma: A comprehensive review of its traditional uses, botany, phytochemistry, pharmacology and toxicology[J]. Pharm Biol, 2019, 57(1): 193-225. doi: 10.1080/13880209.2019.1577466
[17]	SUN Y, LENON GB, YANG AWH. Phellodendri cortex: A phytochemical, pharmacological, and pharmacokinetic review[J]. Evid Based Complement Alternat Med, 2019, 2019: 7621929.
[18]	WANG ZL, WANG S, KUANG Y, et al. A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis[J]. Pharm Biol, 2018, 56(1): 465-484. doi: 10.1080/13880209.2018.1492620
[19]	LI X, TANG ZW, WEN L, et al. Matrine: A review of its pharmacology, pharmacokinetics, toxicity, clinical application and preparation researches[J]. J Ethnopharmacol, 2021, 269: 113682. doi: 10.1016/j.jep.2020.113682
[20]	PAN LL, LI ZZ, WANG YF, et al. Network pharmacology and metabolomics study on the intervention of traditional Chinese medicine Huanglian Decoction in rats with type 2 diabetes mellitus[J]. J Ethnopharmacol, 2020, 258: 112842. doi: 10.1016/j.jep.2020.112842
[21]	章常华, 魏悦, 施旻, 等. 知母黄柏药对对肥胖症大鼠模型降脂作用的实验研究[J]. 时珍国医国药, 2021, 32(4): 773-776. ZHANG CH, WEI Y, SHI M, et al. Experimental study on the lipid-lowering effect of Anemarrhenae Rhizoma-Phelloden-dri Chinensis Cortex herb pair on obesity rat model[J]. Lishizhen Med Mater Med Res, 2021, 32(4): 773-776.
[22]	XIAO SW, LIU C, CHEN MJ, et al. Scutellariae radix and coptidis rhizoma ameliorate glycolipid metabolism of type 2 diabetic rats by modulating gut microbiota and its metabolites[J]. Appl Microbiol Biotechnol, 2020, 104(1): 303-317. doi: 10.1007/s00253-019-10174-w
[23]	SHAO J, LIU Y, WANG H, et al. An integrated fecal microbiome and metabolomics in T2DM rats reveal antidiabetes effects from host-microbial metabolic axis of EtOAc extract from Sophora flavescens[J]. Oxid Med Cell Longev, 2020, 2020: 1805418.
[24]	WANG BT, KONG QM, LI X, et al. A high-fat diet increases gut microbiota biodiversity and energy expenditure due to nutrient difference[J]. Nutrients, 2020, 12(10): 3197. doi: 10.3390/nu12103197
[25]	YUAN XY, XUE J, TAN YX, et al. Albuca bracteate polysaccharides synergistically enhance the anti-tumor efficacy of 5-fluorouracil against colorectal cancer by modulating β-catenin signaling and intestinal flora[J]. Front Pharmacol, 2021, 12: 736627. doi: 10.3389/fphar.2021.736627
[26]	KIM KH, PARK D, JIA BL, et al. Identification and characterization of major bile acid 7α-dehydroxylating bacteria in the human gut[J]. mSystems, 2022, 7(4): e0045522. doi: 10.1128/msystems.00455-22
[27]	ZHAO H, GAO X, LIU ZZ, et al. Sodium alginate prevents non-alcoholic fatty liver disease by modulating the gut-liver axis in high-fat diet-fed rats[J]. Nutrients, 2022, 14(22): 4846. doi: 10.3390/nu14224846
[28]	黄玉普, 吴大章, 王森. 黄芩的药理作用及其药对研究进展[J]. 中国药业, 2022, 31(15): 1-5. https://www.cnki.com.cn/Article/CJFDTOTAL-YYGZ202215030.htm HUANG YP, WU DZ, WANG S. Research progress on pharmacological action of scutellariae Radix and its drug pair[J]. China Pharm, 2022, 31(15): 1-5. https://www.cnki.com.cn/Article/CJFDTOTAL-YYGZ202215030.htm
[29]	彭程程, 姚亮亮, 曾国威, 等. 葛根芩连汤干预胰岛素抵抗大鼠的粪便代谢组及相关菌群研究[J]. 中药新药与临床药理, 2021, 32(12): 1737-1744. PENG CC, YAO LL, ZENG GW, et al. Study on fecal metabolic mechanism and related flora of Gegen Qinlian Decoction in rats with insulin resistance[J]. Tradit Chin Drug Res Clin Pharmacol, 2021, 32(12): 1737-1744.
[30]	ZHAO LJ, MA P, PENG Y, et al. Amelioration of hyperglycaemia and hyperlipidaemia by adjusting the interplay between gut microbiota and bile acid metabolism: Radix Scutellariae as a case[J]. Phytomedicine, 2021, 83: 153477. doi: 10.1016/j.phymed.2021.153477
[31]	FANG YK, YAN C, ZHAO Q, et al. The roles of microbial products in the development of colorectal cancer: A review[J]. Bioengineered, 2021, 12(1): 720-735. doi: 10.1080/21655979.2021.1889109
[32]	GOOSSENS JF, BAILLY C. Ursodeoxycholic acid and cancer: From chemoprevention to chemotherapy[J]. Pharmacol Ther, 2019, 203: 107396.

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(6) / Tables(2)

Get Citation

PDF

XML

Article Metrics

Article views (257) PDF downloads(28)

Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

doi: 10.14148/j.issn.1672-0482.2023.0442

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

doi: 10.14148/j.issn.1672-0482.2023.0442

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content