Volume 39 Issue 4
Apr.  2023
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2023  >  39(4): 365-374
CHONG Ying, LUO Zi-chen, XU Wei-chen, QIAN Gui-ying, SHAN Jin-jun. Effect of Platycodon Grandiflorum Polysaccharide on the Growth and Metabolism of Akkermansia Muciniphila[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(4): 365-374. doi: 10.14148/j.issn.1672-0482.2023.0365
Citation: CHONG Ying, LUO Zi-chen, XU Wei-chen, QIAN Gui-ying, SHAN Jin-jun. Effect of Platycodon Grandiflorum Polysaccharide on the Growth and Metabolism of Akkermansia Muciniphila[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(4): 365-374. doi: 10.14148/j.issn.1672-0482.2023.0365
shu

Effect of Platycodon Grandiflorum Polysaccharide on the Growth and Metabolism of Akkermansia Muciniphila

doi: 10.14148/j.issn.1672-0482.2023.0365
  • 1.

    Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing 210023, China

  • 3.

    Changshu Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou 215500, China

  • Received Date: 2022-08-26
    Available Online: 2023-04-17
    Abstract
  •   OBJECTIVE  To investigate the effect of Platycodon grandiflorum polysaccharides on regulating the growth and metabolism of Akkermansia muciniphila (A.muciniphila).  METHODS  Platycodon grandiflorum polysaccharides were co-incubated with A.muciniphila in vitro and the growth curve was determined; Use LC-MS/MS to determine the content of short-chain fatty acids(SCFAs); Non-targeted metabolomics and non-targeted lipidomics were performed to study the changes of metabolic profiles in A.muciniphila after Platycodon grandiflorum polysaccharides treatment.  RESULTS  Platycodon grandiflorum polysaccharides could promote the growth of A.muciniphila, and increase the content of short-chain fatty acids in A.muciniphila, especially butyrate. Furthermore, Platycodon grandiflorum polysaccharides also remodeled the metabolic profiles of A.muciniphila, as 9 lipids and 18 other metabolites were regulated.  CONCLUSION  Platycodon grandiflorum polysaccharides can enrich A.muciniphila and regulate its metabolism. This study provides a theoretical basis for harnessing Platycodon grandiflorum polysaccharides as prebiotics of A.muciniphila.

     

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