Volume 38 Issue 11
Nov.  2022
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(11): 1027-1034
WANG Chun-hui, CHU Ai-jing, ZHU Zhen-yu, PEI Xiao-hua. Study on the Regulatory Effect of Realgar for External Use on Epithelial Mesenchymal Transformation in Triple Negative Breast Cancer[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(11): 1027-1034. doi: 10.14148/j.issn.1672-0482.2022.1027
Citation: WANG Chun-hui, CHU Ai-jing, ZHU Zhen-yu, PEI Xiao-hua. Study on the Regulatory Effect of Realgar for External Use on Epithelial Mesenchymal Transformation in Triple Negative Breast Cancer[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(11): 1027-1034. doi: 10.14148/j.issn.1672-0482.2022.1027
shu

Study on the Regulatory Effect of Realgar for External Use on Epithelial Mesenchymal Transformation in Triple Negative Breast Cancer

doi: 10.14148/j.issn.1672-0482.2022.1027
  • 1.

    Fangshan Hospital, Beijing University of Chinese Medicine, Beijing 102400, China

  • 2.

    Xiamen Hospital, Beijing University of Chinese Medicine, Xiamen 361001, China

  • 3.

    The Third Clinical College, Beijing University of Chinese Medicine, Beijing 100020, China

  • Received Date: 2022-05-03
    Available Online: 2022-11-19
    Abstract
  •   OBJECTIVE  To investigate the regulatory effect of realgar for external use on epithelial mesenchymal transformation in triple negative breast cancer.  METHODS  Human breast cancer nude mouse xenograft model was established by injecting MDA-MB-231 cells. The mice were randomly divided into model group, realgar group and capecitabine tablet group. Hematoxylin and eosin (HE), immunohistochemistry, Western blot and qPCR were used to observe the effect of realgar on epithelial-mesenchymal transition (EMT) of breast cancer. At the same time, the indexes of blood routine, liver and kidney function were detected.  RESULTS  External using realgar could induce necrosis of breast cancer tissue, the tumor inhibitory rate was 30.2%. Compared with the model group, the liver and kidney functions and blood routine tests of nude mice in realgar group were not significantly abnormal; The immunohistochemical results of tumor tissues suggested that realgar could enhance the expression of E-cadherin and reduce the expression of Vimentin; The results of Western blot and qPCR showed that realgar could enhance the expression of E-cadherin and reduce the expressions of Twist1 and TGF-β1 in tumor tissues (P < 0.05).  CONCLUSION  External using realgar can inhibit the growth of triple negative breast cancer and regulate EMT by increasing the expression of E-cadherin and decreasing the expressions of Vimentin, Twist1 and TGF-β1.

     

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