Volume 38 Issue 8
Aug.  2022
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(8): 727-734
LIU Yi-jing, HU Jing-lu, DU Li-na. The Repellent Effect of Tea Tree Oil Microcapsules Emulsion on Leeches[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(8): 727-734. doi: 10.14148/j.issn.1672-0482.2022.0727
Citation: LIU Yi-jing, HU Jing-lu, DU Li-na. The Repellent Effect of Tea Tree Oil Microcapsules Emulsion on Leeches[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(8): 727-734. doi: 10.14148/j.issn.1672-0482.2022.0727
shu

The Repellent Effect of Tea Tree Oil Microcapsules Emulsion on Leeches

doi: 10.14148/j.issn.1672-0482.2022.0727
  • 1.

    Pharmaceutical College, Shandong University of Traditional Chinese Medicine, Jinan 250355, China

  • 2.

    Beijing Institute of Radiation Medicine, Beijing 100850, China

  • 3.

    College of Pharmacy, Henan University, Kaifeng 475004, China

  • Received Date: 2022-01-21
    Available Online: 2022-08-05
    Abstract
  •   OBJECTIVE  To prepare tea tree oil into microcapsules emulsion, evaluate its repellent effects on leeches and clarify the repellent mechanisms.  METHODS  Tea tree oil aqueous solution was prepared with Tween 20 as solubilizer. Span 80 was used as the emulsifier to prepare tea oil emulsion. Gelatin and sodium alginate were used by complex coacervation method to prepare tea tree oil microcapsules. The particle size, morphology and the binding mode of microcapsules were characterized. The tea tree oil microcapsules emulsion was obtained by dispersing the microcapsules into the tea tree oil emulsion. The filter-paper ring method and the repellent test in water were used to evaluate the repellent effects and time of the tea tree oil microcapsules emulsion, tea oil aqueous solution, blank microcapsules emulsion and the positive drugs on leeches. The activities of acetylcholinesterase (AchE), glutathione S-transferase (GST) and carboxylesterase (CarE) activities in vivo were measured to clarify the repellent mechanisms of tea tree oil microcapsules emulsion on leeches.  RESULTS  The optimal ratio of gelatin and sodium alginate with high coacervation yield was obtained. The microcapsules with uniform particle size, uneven size and high encapsulation efficiency were successfully prepared. According to the orthogonal design, the optimal formulation was obtained, and the tea tree oil microcapsules emulsion was uniformly dispersed and stable. The microcapsules emulsion could effectively repel leeches and prolong the repelling time. It was clarified that tea tree oil microcapsules emulsion played a repellent role by increasing AchE activity and inhibiting GST and CarE activities in vivo.  CONCLUSION  The prepared tea tree oil microcapsules emulsion can avoid the volatilization of tea tree oil, which can prolong the repelling time. In summary, this study provides a new idea for the development of natural essential oil as the repellents with broad market prospects.

     

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    • References(22)
  • [1]
    TESSLER M, MARANCIK D, CHAMPAGNE D, et al. Marine leech anticoagulant diversity and evolution[J]. J Parasitol, 2018, 104(3): 210-220. doi: 10.1645/17-64
    [2]
    HANNAN MJ, HOQUE MM. Leech infestation in children through body orifices: Experience in a hospital in Bangladesh[J]. World J Surg, 2012, 36(9): 2090-2092. doi: 10.1007/s00268-012-1633-x
    [3]
    DHAKAD AK, PANDEY VV, BEG S, et al. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review[J]. J Sci Food Agric, 2018, 98(3): 833-848. doi: 10.1002/jsfa.8600
    [4]
    TITOUHI F, AMRI M, MESSAOUD C, et al. Protective effects of three Artemisia essential oils against Callosobruchus maculatus and Bruchus rufimanus (Coleoptera: Chrysomelidae) and the extended side-effects on their natural enemies[J]. J Stored Prod Res, 2017, 72: 11-20. doi: 10.1016/j.jspr.2017.02.007
    [5]
    董菲, 田玉红, 黄琼, 等. 植物精油对害虫的驱避与毒杀活性的应用研究进展[J]. 农药, 2018, 57(11): 781-784, 788. https://www.cnki.com.cn/Article/CJFDTOTAL-NYZZ201811001.htm

    DONG F, TIAN YH, HUANG Q, et al. Advance and application of repellent and insecticidal activities against insect pests by essential oils[J]. Agrochemicals, 2018, 57(11): 781-784, 788. https://www.cnki.com.cn/Article/CJFDTOTAL-NYZZ201811001.htm
    [6]
    尤龙, 王建全, 任士伟. 国内植物精油提取工艺研究进展[J]. 山东化工, 2019, 48(11): 28-29. doi: 10.3969/j.issn.1008-021X.2019.11.012

    YOU L, WANG JQ, REN SW. Domestic progress in extraction of plant essential oil[J]. Shandong Chem Ind, 2019, 48(11): 28-29. doi: 10.3969/j.issn.1008-021X.2019.11.012
    [7]
    MARTINE V. Essential oils: Renewal of interest and toxicity[J]. Eur J Dermatol, 2010, 20(6): 685-692.
    [8]
    LEE MY. Essential oils as repellents against arthropods[J]. Biomed Res Int, 2018, 2018: 6860271.
    [9]
    MUHOZA B, XIA SQ, WANG XJ, et al. Microencapsulation of essential oils by complex coacervation method: Preparation, thermal stability, release properties and applications[J]. Crit Rev Food Sci Nutr, 2022, 62(5): 1363-1382. doi: 10.1080/10408398.2020.1843132
    [10]
    MENDANHA DV, MOLINA ORTIZ SE, FAVARO-TRINDADE CS, et al. Microencapsulation of casein hydrolysate by complex coacervation with SPI/pectin[J]. Food Res Int, 2009, 42(8): 1099-1104. doi: 10.1016/j.foodres.2009.05.007
    [11]
    DE MATOS EF, SCOPEL BS, DETTMER A. Citronella essential oil microencapsulation by complex coacervation with leather waste gelatin and sodium alginate[J]. J Environ Chem Eng, 2018, 6(2): 1989-1994. doi: 10.1016/j.jece.2018.03.002
    [12]
    李宏英, 王鸿博, 傅佳佳, 等. 明胶-海藻酸钠制备薄荷油微胶囊的工艺优化[J]. 高分子材料科学与工程, 2019, 35(9): 142-149. https://www.cnki.com.cn/Article/CJFDTOTAL-GFZC201909022.htm

    LI HY, WANG HB, FU JJ, et al. Optimization of preparation technology of peppermint oil microcapsules in gelatin-sodium alginate[J]. Polym Mater Sci Eng, 2019, 35(9): 142-149. https://www.cnki.com.cn/Article/CJFDTOTAL-GFZC201909022.htm
    [13]
    赵树超, 王萍, 黄振东, 等. 6种常用市售宠物驱避剂对长角血蜱的驱避效果[J]. 中华卫生杀虫药械, 2018, 24(6): 527-529, 533. https://www.cnki.com.cn/Article/CJFDTOTAL-WSSC201806003.htm

    ZHAO SC, WANG P, HUANG ZD, et al. Effect of six commonly used pet repellents on Haemaphysalis longicornis[J]. Chin J Hyg Insectic Equip, 2018, 24(6): 527-529, 533. https://www.cnki.com.cn/Article/CJFDTOTAL-WSSC201806003.htm
    [14]
    YANG JL, ZHOU ZY, LIANG Y, et al. Sustainable preparation of microcapsules with desirable stability and bioactivity using phosphonium ionic liquid as a functional additive[J]. ACS Sustainable Chem Eng, 2020, 8(35): 13440-13448. doi: 10.1021/acssuschemeng.0c04245
    [15]
    YU YJ, SI ZH, CHEN SJ, et al. Facile synthesis of polyaniline-sodium alginate nanofibers[J]. Langmuir, 2006, 22(8): 3899-3905. doi: 10.1021/la051911v
    [16]
    倪张根, 王秋萍, 张瑞萍, 等. 复合凝聚法制备芳香微胶囊[J]. 纺织科学与工程学报, 2020, 37(3): 25-32. https://www.cnki.com.cn/Article/CJFDTOTAL-CDFZ202003006.htm

    NI ZG, WANG QP, ZHANG RP, et al. Preparation of aromatic microcapsules by Complex Coacervation method[J]. J Text Sci Eng, 2020, 37(3): 25-32. https://www.cnki.com.cn/Article/CJFDTOTAL-CDFZ202003006.htm
    [17]
    GUO DH, LUO JP, ZHOU YN, et al. ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq data[J]. BMC Bioinformatics, 2017, 18(1): 330. doi: 10.1186/s12859-017-1741-6
    [18]
    DOS SANTOS MIRON D, CRESTANI M, ROSA SHETTINGER M, et al. Effects of the herbicides clomazone, quinclorac, and metsulfuron methyl on acetylcholinesterase activity in the silver catfish (Rhamdia quelen) (Heptapteridae)[J]. Ecotoxicol Environ Saf, 2005, 61(3): 398-403. doi: 10.1016/j.ecoenv.2004.12.019
    [19]
    YANG JL, WEI XM, XU J, et al. A Sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants[J]. Fish Shellfish Immunol, 2012, 32(6): 1198-1204. doi: 10.1016/j.fsi.2012.03.010
    [20]
    王蕊, 姜剑伟, 李燕. 哺乳动物羧酸酯酶在药物代谢中的作用[J]. 药学学报, 2018, 53(2): 186-191. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB201802004.htm

    WANG R, JIANG JW, LI Y. Role of carboxylesterase in drug metabolism[J]. Acta Pharm Sin, 2018, 53(2): 186-191. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB201802004.htm
    [21]
    ZHANG JQ, GE PT, LI DQ, et al. Two homologous carboxylesterase genes from Locusta migratoria with different tissue expression patterns and roles in insecticide detoxification[J]. J Insect Physiol, 2015, 77: 1-8. doi: 10.1016/j.jinsphys.2015.03.013
    [22]
    HATFIELD MJ, POTTER PM. Carboxylesterase inhibitors[J]. Expert Opin Ther Pat, 2011, 21(8): 1159-1171. doi: 10.1517/13543776.2011.586339
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