Volume 38 Issue 6
Jun.  2022
Turn off MathJax
Article Contents
Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(6): 527-533
ZHAO Meng, LIU Zhuo-ya, YU Jia-min, WANG Rui, FAN Ming-jie, QIAO Hong-zhi. Study on the Formulation and in vitro Release of Evodiamine-Loaded Ginger-Derived Extracellular Vesicle-Like Nanoparticles[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(6): 527-533. doi: 10.14148/j.issn.1672-0482.2022.0527
Citation: ZHAO Meng, LIU Zhuo-ya, YU Jia-min, WANG Rui, FAN Ming-jie, QIAO Hong-zhi. Study on the Formulation and in vitro Release of Evodiamine-Loaded Ginger-Derived Extracellular Vesicle-Like Nanoparticles[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(6): 527-533. doi: 10.14148/j.issn.1672-0482.2022.0527
shu

Study on the Formulation and in vitro Release of Evodiamine-Loaded Ginger-Derived Extracellular Vesicle-Like Nanoparticles

doi: 10.14148/j.issn.1672-0482.2022.0527
  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, Nanjing 210023, China

  • 3.

    Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing 210023, China

  • Received Date: 2022-03-01
    Available Online: 2022-06-07
    Abstract
  •   OBJECTIVE  Lipids from ginger-derived extracellular vesicle-like nanoparticles (EVNs) were extracted and used to prepare liposomes containing evodiamine (EVO) to improve its druggability.  METHODS  EVNs from ginger were separated by differential centrifugation, and the lipid extraction solvent was screened. Liposomes loaded with EVO (EVO@Lipo) were prepared by thin film dispersion method. The formulation and preparation process of liposome were optimized by orthogonal test with encapsulation rate as evaluation index. EVO@Lipo was characterized by particle size and potential analysis, differential calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), and the drug release behavior of EVO@Lipo was investigated in vitro.  RESULTS  Trichloromethane, methanol-trichloromethane and ethanol-dichloromethane were screened as lipid extraction solvents. The optimized preparation conditions were methanol-trichloromethane (2∶1) as lipid extraction solvent, drug to lipid ratio of 1∶50, ultrasonic conditions of 60 W, 15 min. The encapsulation efficiency of EVO@Lipo was 88.21%, the average particle size was 194.9 nm, the PDI was 0.22, and the Zeta potential was -35.3 mV. Accumulated evidence suggests that EVO@Lipo is not a physical mixture of drugs and lipids. In vitro release experiments showed that EVO@Lipo could delay drug release.  CONCLUSION  Lipids from ginger EVNs can be used to load hydrophobic drug EVO, improve its solubility, and have a certain sustained release effect.

     

    • FullText(HTML)
  • loading
    • References(17)
  • [1]
    任海霞, 罗和生, 唐勤彩, 等. 吴茱萸碱在胃肠疾病中的研究进展[J]. 医学综述, 2018, 24(16): 3253-3258. doi: 10.3969/j.issn.1006-2084.2018.16.025

    REN HX, LUO HS, TANG QC, et al. Research progress of evodiamine in gastrointestinal diseases[J]. Med Recapitulate, 2018, 24(16): 3253-3258. doi: 10.3969/j.issn.1006-2084.2018.16.025
    [2]
    董丹丹, 焦红军, 郝海军, 等. 吴茱萸碱纳米结构脂质载体处方优化和SD大鼠体内口服药动学研究[J]. 中草药, 2022, 53(1): 60-70. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202201009.htm

    DONG DD, JIAO HJ, HAO HJ, et al. Optimization of evodiamine nanostructured lipid carriers and oral pharmacokinetics study in SD rats[J]. Chin Tradit and Herb Drugs, 2022, 53(1): 60-70. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202201009.htm
    [3]
    李姝影. 吴茱萸碱脂质体的制备和体内外评价[D]. 济南: 山东中医药大学, 2015.

    LI SY. Preparation of evodiamine liposome and the evaluation in vitro and in vivo[D]. Jinan: Shandong University of Traditional Chinese Medicine, 2015.
    [4]
    张雪. 吴茱萸碱纳米脂质体对四氯化碳或硫代乙酰胺诱导小鼠肝脏纤维化的作用及机制研究[D]. 重庆: 重庆医科大学, 2020.

    ZHANG X. Effect and mechanism of evodiamine nanoliposomes on carbon tetrachloride or thioacetamide-induced liver fibrosis in mice[D]. Chongqing: Chongqing University of Medical Sciences, 2020.
    [5]
    杨婕, 刘宏明, 陈云, 等. 吴茱萸碱脂质纳米粒的药代动力学和在体肠吸收特性研究[J]. 中国药科大学学报, 2020, 51(6): 696-701. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYD202006008.htm

    YANG J, LIU HM, CHEN Y, et al. Pharmacokinetics and in situ intestinal absorption of evodiamine lipidic nanoparticle[J]. J Chin Pharm Univ, 2020, 51(6): 696-701. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYD202006008.htm
    [6]
    赵梦, 李思敏, 张蕾, 等. 植物来源囊泡及其生物医学应用研究进展[J]. 药学学报, 2021, 56(8): 2039-2047, 2036. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB202108004.htm

    ZHAO M, LI SM, ZHANG L, et al. Research progress of plant-derived vesicles and their biomedical applications[J]. Acta Pharm Sin, 2021, 56(8): 2039-2047, 2036. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB202108004.htm
    [7]
    CONG MH, TAN SY, LI SM, et al. Technology insight: Plant-derived vesicles-How far from the clinical biotherapeutics and therapeutic drug carriers?[J]. Adv Drug Deliv Rev, 2022, 182: 114108. doi: 10.1016/j.addr.2021.114108
    [8]
    TENG Y, MU JY, HU X, et al. Grapefruit-derived nanovectors deliver miR-18a for treatment of liver metastasis of colon cancer by induction of M1 macrophages[J]. Oncotarget, 2016, 7(18): 25683-25697. doi: 10.18632/oncotarget.8361
    [9]
    WANG QL, ZHUANG XY, MU JY, et al. Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids[J]. Nat Commun, 2013, 4: 1867. doi: 10.1038/ncomms2886
    [10]
    ZHUANG XY, TENG Y, SAMYKUTTY A, et al. Grapefruit-derived nanovectors delivering therapeutic miR17 through an intranasal route inhibit brain tumor progression[J]. Mol Ther, 2016, 24(1): 96-105. doi: 10.1038/mt.2015.188
    [11]
    YANG CH, ZHANG MZ, LAMA S, et al. Natural-lipid nanoparticle-based therapeutic approach to deliver 6-shogaol and its metabolites M2 and M13 to the colon to treat ulcerative colitis[J]. J Control Release, 2020, 323: 293-310. doi: 10.1016/j.jconrel.2020.04.032
    [12]
    TENG Y, REN Y, SAYED M, et al. Plant-derived exosomal microRNAs shape the gut microbiota[J]. Cell Host Microbe, 2018, 24(5): 637-652. doi: 10.1016/j.chom.2018.10.001
    [13]
    ZHANG MZ, VIENNOIS E, PRASAD M, et al. Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer[J]. Biomaterials, 2016, 101: 321-340. doi: 10.1016/j.biomaterials.2016.06.018
    [14]
    SUNDARAM K, MiLLER DP, KUMAR A, et al. Plant-derived exosomal nanoparticles inhibit pathogenicity of porphyromonas gingivalis[J]. iScience, 2019, 21: 308-327. doi: 10.1016/j.isci.2019.10.032
    [15]
    ZHUANG XY, DENG ZB, MU JY, et al. Ginger-derived nanoparticles protect against alcohol-induced liver damage[J]. J Extracell Vesicles, 2015, 4: 28713. doi: 10.3402/jev.v4.28713
    [16]
    ZHANG MZ, XIAO B, WANG H, et al. Edible ginger-derived nano-lipids loaded with doxorubicin as a novel drug-delivery approach for colon cancer therapy[J]. Mol Ther, 2016, 24(10): 1783-1796. doi: 10.1038/mt.2016.159
    [17]
    HETTICH BF, BADER JJ, LEROUX JC. Encapsulation of hydrophilic compounds in small extracellular vesicles: loading capacity and impact on vesicle functions[J]. Adv Healthc Mater, 2022, 11(5): e2100047. doi: 10.1002/adhm.202100047
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(8)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (315) PDF downloads(18) Cited by()
    Proportional views
    Related

    Copyright © Journal of Nanjing University of traditional Chinese Medicine

    Sponsored by：Nanjing University of traditional Chinese MedicineAddress：No.138 xianlin Avenue, Qixia District, Nanjing cityChina Pos：210023

    Tel：025-85811935Email：xb@njucm.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return