Volume 38 Issue 6
Jun.  2022
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(6): 520-526
ZENG Ping, YAN Yu-mei, YU Hong-li, WU Hao, TAO Xing-bao, XIE Yu-wei, CHENG Yan-qiu, WANG Cai-xia, WANG He-peng, CHEN Jin-song. Effect of Arisaema Cum Bile on Brain Tissue Injury and Inflammation in Febrile Seizures Model Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(6): 520-526. doi: 10.14148/j.issn.1672-0482.2022.0520
Citation: ZENG Ping, YAN Yu-mei, YU Hong-li, WU Hao, TAO Xing-bao, XIE Yu-wei, CHENG Yan-qiu, WANG Cai-xia, WANG He-peng, CHEN Jin-song. Effect of Arisaema Cum Bile on Brain Tissue Injury and Inflammation in Febrile Seizures Model Mice[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(6): 520-526. doi: 10.14148/j.issn.1672-0482.2022.0520
shu

Effect of Arisaema Cum Bile on Brain Tissue Injury and Inflammation in Febrile Seizures Model Mice

doi: 10.14148/j.issn.1672-0482.2022.0520
  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    Jinghua Pharmaceutical Group Co., Ltd., Nantong 226000, China

  • 3.

    Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210023, China

  • 4.

    Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China

  • 5.

    Sichuan Qianyuan Chinese Herbal Pieces Co., Ltd., Guanghan 618304, China

  • Received Date: 2022-01-17
    Available Online: 2022-06-07
    Abstract
  •   OBJECTIVE  To study the efficacy of Arisaema Cum Bile on antagonizing brain tissue damage and inflammation in febrile seizures model mice.  METHODS  Mice model of febrile seizures was established by using dry yeast suspension and pentylenetetrazol solution, and the effects of low, medium and high dose groups of Arisaema Cum Bile water extract on rectal temperature, convulsion latency and convulsion duration of model mice were investigated. HE staining was used to observe the morphology of mice hippocampal neurons. ELISA was used to detect the levels of serum cAMP and PGE2. Western blot method was used to detect the protein expressions of COX-2, iNOS, GFAP and GABAAR in brain tissue. The qPCR method was used to detect inflammatory factors IL-1β, IL-6, TNF-α mRNA expression in hippocampus tissue.  RESULTS  Compared with the model group, the high-dose Arisaema Cum Bile group could reduce the rectal temperature (P < 0.05), prolong the convulsion latency (P < 0.05), shorten the duration of convulsions (P < 0.01), and up-regulate the expression of GABAAR protein (P < 0.05); At the same time, it decreased the levels of serum cAMP and PGE2 (P < 0.01), and improved the morphological changes of hippocampal neurons, down-regulated the expressions of COX-2, iNOS and GFAP protein in the brain tissue (P < 0.05), and decreased the expression levels of IL-1β, IL-6 and TNF-α mRNA in the hippocampus (P < 0.05).  CONCLUSION  Arisaema Cum Bile has a good antipyretic convulsant effect, and its mechanism may be related to antipyretic and antagonizing brain tissue inflammation.

     

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