Volume 38 Issue 5
May  2022
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(5): 375-381
WANG He-peng, YU Hong-li, WU Hao, XIE Yu-wei, TAO Xing-bao, ZENG Ping, CHENG Yan-qiu, WANG Cai-xia, LIU Bing-bing, ZHANG Ping. Effect of Arisaematis Rhizoma Processed on the Contents of Lectin Protein and Calcium Oxalate Crystal as Toxic Components[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(5): 375-381. doi: 10.14148/j.issn.1672-0482.2022.0375
Citation: WANG He-peng, YU Hong-li, WU Hao, XIE Yu-wei, TAO Xing-bao, ZENG Ping, CHENG Yan-qiu, WANG Cai-xia, LIU Bing-bing, ZHANG Ping. Effect of Arisaematis Rhizoma Processed on the Contents of Lectin Protein and Calcium Oxalate Crystal as Toxic Components[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(5): 375-381. doi: 10.14148/j.issn.1672-0482.2022.0375
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Effect of Arisaematis Rhizoma Processed on the Contents of Lectin Protein and Calcium Oxalate Crystal as Toxic Components

doi: 10.14148/j.issn.1672-0482.2022.0375
  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210023, China

  • 3.

    Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China

  • 4.

    National Institutes for Food and Drug Control, Beijing 100050, China

  • Received Date: 2022-01-17
    Abstract
  •   OBJECTIVE  The present study was aimed to investigate the effect of Arisaematis Rhizoma processed on the contents of endogenic toxic components and to establish the detection method.  METHODS  HPLC was applied to determinate the content of calcium oxalate. The separation was developed on Megres C18 column (250 mm×4.6 mm, 5 μm) by gradient elution with water containing 0.5% phosphoric acid as the moble phase at the flow rate of 0.8 mL·min-1, the column temperature of 25 ℃, and the detection wavelength of 220 nm. Western blot was applied to detect the content of lectin protein.  RESULTS  The contents of calcium oxalate of all batches of raw Arisaematis Rhizoma was 0.78%-2.33%, and average content was 1.43%;The contents of calcium oxalate of all batches of processed Arisaematis Rhizoma were 0.29%-0.98%, and average content was 0.60%; After processing, the decreasing amplitude of calcium oxalate were 25.6%-78.3%. No lectin protein was detected in all batches of processed Arisaematis Rhizoma.  CONCLUSION  The contents of toxic components, lectin protein and calcium oxalate, were decreased in Arisaematis Rhizoma after processed. The detection method of lectin protein and calcium oxalate can be used to detect toxic components of multi-batch Arisaematis Rhizoma, and can provide reference for the establishment of toxicity control index of Arisaematis Rhizoma. It is stable and reliable that consider the limit of lectin protein as toxicity control index of Arisaematis Rhizoma.

     

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    • References(10)
  • [1]
    国家中医药管理局. 中华本草: 精华本[M]. 上海: 上海科学技术出版社, 1998.

    National Administration of Traditional Chinese Medicine. Chinese Materia Medica: Essence Edition[M]. Shanghai: Shanghai science and technology press, 1998.
    [2]
    吴皓, 钟凌云. 天南星科有毒中药刺激性作用比较研究[J]. 中国中药杂志, 2008, 33(4): 380-384. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY200804010.htm

    WU H, ZHONG LY. Study on irritation of calcium oxalate crystal in Araceae plants[J]. China J Chin Mater Med, 2008, 33(4): 380-384. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY200804010.htm
    [3]
    葛秀允, 吴皓. 天南星科有毒中药毒针晶的组成成分分析[J]. 药物分析杂志, 2010, 30(2): 190-193. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201002003.htm

    GE XY, WU H. Analysis of the composition of poisonous raphides in Araceae plant[J]. Chin J Pharm Anal, 2010, 30(2): 190-193. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201002003.htm
    [4]
    刘先琼, 吴皓, 郁红礼, 等. 半夏、掌叶半夏及禹白附凝集素蛋白的刺激性研究[J]. 中国实验方剂学杂志, 2011, 17(18): 244-247. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201118071.htm

    LIU XQ, WU H, YU HL, et al. Irritability of agglutinin from Pinellia ternata breit, Pinellia pedtaisecta schott and Typhonium giganteum engl[J]. Chin J Exp Tradit Med Formulae, 2011, 17(18): 244-247. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201118071.htm
    [5]
    LIU XQ, WU H, YU HL, et al. Purification of a lectin from Arisaema erubescens (wall. ) schott and its pro-inflammatory effects[J]. Molecules, 2011, 16(11): 9480-9494. doi: 10.3390/molecules16119480
    [6]
    国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 58.

    Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China: Ⅰ[M]. Beijing: China medical and technology press, 2020: 58.
    [7]
    葛秀允. 天南星科有毒中药刺激性毒性成分及矾制解毒共性机制研究[D]. 南京: 南京中医药大学, 2009.

    GE XY. The poisonous components and common mechanism of detoxification processing of Araceae herbal medicine[D]. Nanjing: Nanjing University of Chinese Medicine, 2009.
    [8]
    王卫. 天南星科4种有毒中药凝集素蛋白促炎作用机制及炮制的影响[D]. 南京: 南京中医药大学, 2019.

    WANG W. Study on the pro-inflammatory mechanism of four lectins in Araceae and its effect by processing[D]. Nanjing: Nanjing University of Chinese Medicine, 2019.
    [9]
    郁红礼, 王卫, 吴皓, 等. 炮制对天南星科4种有毒中药毒性成分凝集素蛋白的影响[J]. 中国中药杂志, 2019, 44(24): 5398-5404. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201924025.htm

    YU HL, WANG W, WU H, et al. Effect of processing on toxic components lectin from four kinds of Araceae toxic medicines[J]. China J Chin Mater Med, 2019, 44(24): 5398-5404. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201924025.htm
    [10]
    YU HL, PAN YZ, WU H, et al. The alum-processing mechanism attenuating toxicity of Araceae Pinellia ternata and Pinellia pedatisecta[J]. Arch Pharmacal Res, 2015, 38(10): 1810-1821.
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