Volume 38 Issue 4
Apr.  2022
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2022  >  38(4): 315-322
XU Xin-yi, YANG Xin, LIANG Guo-qiang, ZHU Hui-ping, HUANG Xue, WANG Ai-min, SUN Hong-wen. Effect of Quyu Humo Paste on the Repair of Esophageal Mucosa in Rats with Reflux Esophagitis by Regulating NLRP3/Caspase-1 Signaling Pathway[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(4): 315-322. doi: 10.14148/j.issn.1672-0482.2022.0315
Citation: XU Xin-yi, YANG Xin, LIANG Guo-qiang, ZHU Hui-ping, HUANG Xue, WANG Ai-min, SUN Hong-wen. Effect of Quyu Humo Paste on the Repair of Esophageal Mucosa in Rats with Reflux Esophagitis by Regulating NLRP3/Caspase-1 Signaling Pathway[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(4): 315-322. doi: 10.14148/j.issn.1672-0482.2022.0315
shu

Effect of Quyu Humo Paste on the Repair of Esophageal Mucosa in Rats with Reflux Esophagitis by Regulating NLRP3/Caspase-1 Signaling Pathway

doi: 10.14148/j.issn.1672-0482.2022.0315
  • 1.

    Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215007, China

  • 2.

    Department of Traditional Chinese Medicine, Suzhou Science & Technology Town Hospital, Suzhou 215000, China

  • 3.

    Suzhou Academy of Wumen Chinese Medicine, Suzhou 215007, China

  • Received Date: 2021-05-30
    Available Online: 2022-04-13
    Abstract
  •   OBJECTIVE  From the perspective of esophageal mucosal barrier repair, to explore the effect of Quyu Humo paste on nucleotide binding oligomeric domain-like receptor protein 3 (NLRP3)/aspartate specific cysteine protease-1 (Caspase-1) signal pathway in rats with reflux esophagitis (RE), and to reveal the mechanism of Quyu Humo paste in the treatment of RE.  METHODS  Rats were randomly divided into blank group (n=10), model group (n=10), western medicine group (n=10), low dose Quyu Humo paste group (n=10), middle dose Quyu Humo paste group (n=10) and high dose Quyu Humo paste group (n=10). Except the blank group, the RE rat model was established by "4.2 mm pyloric clip+2/3 gastric fundus ligation" in the other 5 groups. The rats in the blank group and model group were given starch+distilled water, each doses of Quyu Humo paste groups were treated with Quyu Humo paste of 0.54, 1.62, 4.86 g·kg-1, respectively, and western medicine group were given aluminum and magnesium plus suspension for 14 d. On the 15th day, the rats were killed and the esophageal tissues were taken out. The contents of Caspase-1, IL-1β and IL-18 in serum were detected by ELISA method, the protein expressions of NLRP3 and Caspase-1 in esophageal tissue were detected by Western blot, and the pathological changes of esophageal tissue were observed under light microscope with HE staining.  RESULTS  The protein expressions of Caspase-1, IL-1β, IL-18 in serum and NLRP3 and Caspase-1 in esophageal tissue in the model group were significantly higher than those in the blank group (P < 0.001). Compared with the model group, the contents of Caspase-1, IL-1β, IL-18 in serum and the protein expressions of NLRP3 and Caspase-1 in esophageal tissue decreased significantly in western medicine group, middle and high dose Quyu Humo paste groups (P < 0.001). Compared with the western medicine group, the protein expressions of NLRP3 and Caspase-1 in esophageal tissue and Caspase-1 in serum significantly decreased in the high dose Quyu Humo paste group (P < 0.01, P < 0.001).  CONCLUSION  Quyu Humo paste can improve the injury of esophageal epithelium, repair esophageal mucosa and promote the healing of reflux esophagitis in rats by inhibiting the excessive activation of NLRP3 signal pathway, down-regulating the expression of Caspase-1, reducing the levels of IL-1β and IL-18, and antagonizing esophageal inflammation.

     

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    • References(30)
  • [1]
    TRIPATHI M, STREUTKER CJ, MARGINEAN EC. Relevance of histology in the diagnosis of reflux esophagitis[J]. Ann NY Acad Sci, 2018, 1434(1): 94-101. doi: 10.1111/nyas.13742
    [2]
    丁大洪. 反流性食管病(炎)诊断及治疗方案(试行)[J]. 中华消化内镜杂志, 1999, 16(6): 326. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHXH199906001.htm

    DING DH. Reflux esophageal disease (inflammation) diagnosis and treatment plan (trial)[J]. Chin J Dig Endosc, 1999, 16(6): 326. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHXH199906001.htm
    [3]
    JUNG HK. Epidemiology of gastroesophageal reflux disease in Asia: A systematic review[J]. J Neurogastroenterol Motil, 2011, 17(1): 14-27. doi: 10.5056/jnm.2011.17.1.14
    [4]
    ZOU DW, HE J, MA XQ, et al. Epidemiology of symptom-defined gastroesophageal reflux disease and reflux esophagitis: The systematic investigation of gastrointestinal diseases in China (SILC)[J]. Scand J Gastroenterol, 2011, 46(2): 133-141. doi: 10.3109/00365521.2010.521888
    [5]
    王瀛峰, 张继全, 吴飞, 等. 胃食管反流病的中西医发病机制及临床治疗的研究进展[J]. 世界华人消化杂志, 2013, 21(34): 3821-3827. https://www.cnki.com.cn/Article/CJFDTOTAL-XXHB201334011.htm

    WANG YF, ZHANG JQ, WU F, et al. Update on the pathogenesis and clinical treatment of gastroesophageal reflux disease[J]. World Chin J Dig, 2013, 21(34): 3821-3827. https://www.cnki.com.cn/Article/CJFDTOTAL-XXHB201334011.htm
    [6]
    FREEDBERG DE, KIM LS, YANG YX. The risks and benefits of long-term use of proton pump inhibitors: Expert review and best practice advice from the American gastroenterological association[J]. Gastroenterology, 2017, 152(4): 706-715. doi: 10.1053/j.gastro.2017.01.031
    [7]
    李健, 唐芙爱. 胃粘膜保护剂的临床应用评价[J]. 中国医院用药评价与分析, 2003, 3(3): 133-137. doi: 10.3969/j.issn.1672-2124.2003.03.002

    LI J, TANG FA. Evaluation of clinical application of gastroduodenal mucosal protective agents[J]. Eval Anal Drug Use Hosp China, 2003, 3(3): 133-137. doi: 10.3969/j.issn.1672-2124.2003.03.002
    [8]
    陆为民, 徐丹华, 周晓波. 国医大师徐景藩教授诊治胃食管反流病的经验[J]. 中华中医药杂志, 2013, 28(3): 703-705. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY201303034.htm

    LU WM, XU DH, ZHOU XB. Experience of professor XU Jing-fan in treating gastroesophageal reflux disease[J]. China J Tradit Chin Med Pharm, 2013, 28(3): 703-705. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY201303034.htm
    [9]
    张迎春, 孙宏文. 徐景藩临床应用糊剂护膜法治疗脾胃疾病经验撷菁[J]. 中医药临床杂志, 2020, 32(10): 1822-1826. https://www.cnki.com.cn/Article/CJFDTOTAL-AHLC202010008.htm

    ZHANG YC, SUN HW. Experience of XU Jingfan in clinical application of protective membrane therapy in treating spleen and stomach diseases[J]. Clin J Tradit Chin Med, 2020, 32(10): 1822-1826. https://www.cnki.com.cn/Article/CJFDTOTAL-AHLC202010008.htm
    [10]
    SOUZA RF, BAYEH L, SPECHLER SJ, et al. A new paradigm for GERD pathogenesis. Not acid injury, but cytokine-mediated inflammation driven by HIF-2α: A potential role for targeting HIF-2α to prevent and treat reflux esophagitis[J]. Curr Opin Pharmacol, 2017, 37: 93-99.
    [11]
    FU Q, WU J, ZHOU XY, et al. NLRP3/caspase-1 pathway-induced pyroptosis mediated cognitive deficits in a mouse model of Sepsis-associated encephalopathy[J]. Inflammation, 2019, 42(1): 306-318. doi: 10.1007/s10753-018-0894-4
    [12]
    邹方明. 慢性反流性食管炎大鼠模型的建立及5-羟色胺4受体激动剂的抗食管黏膜炎症作用[D]. 福州: 福建医科大学, 2012.

    ZOU FM. Establishment of a chronic acid reflux esophagitis rat model and the anti-inflammatory effect of 5-hydroxytryptamine Ⅳ agonist on the chronic esophageal inflammation[D]. Fuzhou: Fujian Medical University, 2012.
    [13]
    徐叔云. 药理实验方法学[M]. 3版. 北京: 人民卫生出版社, 2002.

    XU SY. Experimental Methodology of Pharmacology[M]. 3th ed. Beijing: People's medical publishing house, 2002.
    [14]
    张声生, 朱生樑, 王宏伟, 等. 胃食管反流病中医诊疗专家共识意见(2017)[J]. 中国中西医结合消化杂志, 2017, 25(5): 321-326. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXPW201705001.htm

    ZHANG SS, ZHU SL, WANG HW, et al. Expert consensus on diagnosis and treatment of gastroesophageal reflux disease (2017)[J]. Chin J Integr Tradit West Med Dig, 2017, 25(5): 321-326. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXPW201705001.htm
    [15]
    王瑞, 唐艳萍, 弓艳霞, 等. 应激对反流性食管炎模型大鼠食管下段pH值及黏膜血流的影响[J]. 中国中西医结合外科杂志, 2016, 22(1): 42-44. doi: 10.3969/j.issn.1007-6948.2016.01.012

    WANG R, TANG YP, GONG YX, et al. The influence of pH values and blood flow of lower esophageal in reflux esophagitis model rat by stress[J]. Chin J Surg Integr Tradit West Med, 2016, 22(1): 42-44. doi: 10.3969/j.issn.1007-6948.2016.01.012
    [16]
    叶天士. 临证指南医案[M]. 上海: 上海人民出版社, 1959: 543, 572, 600.

    YE TS. A Guide to Clinical Practice with Medical Record[M]. Shanghai: Shanghai people's publishing house, 1959: 543, 572, 600.
    [17]
    徐景藩. 徐景藩脾胃病临证经验集粹[M]. 陆为民, 徐丹华, 罗斐和, 整理. 北京: 科学出版社, 2010: 161.

    XU JP. Xu Jingfan's Clinical Experience of Spleen and Stomach Diseases[M]. LU WM, XU DH, LUO FH, collated. Beijing: Science press, 2010: 161.
    [18]
    黄元御. 黄元御医书全集·玉楸药解[M]. 北京: 中医古籍出版社, 2016: 1874.

    HUANG YY. The Complete Collection of Huang Yuanyu's Medical Records: Explanation of Medicinals from Yuqiu[M]. Beijing: Publishing house of ancient Chinese medical books, 2016: 1874.
    [19]
    国家中医心血管病临床医学研究中心, 中国医师协会中西医结合医师分会, 中国中西医结合学会活血化瘀专业委员会, 等. 三七总皂苷制剂临床应用中国专家共识[J]. 中国中西医结合杂志, 2021, 41(10): 1157-1167. https://www.cnki.com.cn/Article/CJFDTOTAL-ZZXJ202110001.htm

    National Clinical Research Center for Cardiovascular Diseases of Traditional Chinese Medicine, Branch of Integrated Traditional Chinese and Western Medicine, Chinese Physicians' Association, Professional Committee of Activating Blood and Dissolving Stasis of Chinese Association of Integrative Medicine, et al. Chinese expert consensus on clinical application of total saponins of Panax notognseng[J]. Chin J Integr Tradit West Med, 2021, 41(10): 1157-1167. https://www.cnki.com.cn/Article/CJFDTOTAL-ZZXJ202110001.htm
    [20]
    王东帆, 王婷, 吕泽中, 等. 三七总皂苷抑制自然衰老大鼠神经细胞凋亡作用的研究[J]. 中药新药与临床药理, 2019, 30(10): 1159-1164. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY201910003.htm

    WANG DF, WANG T, LYU ZZ, et al. Effects of Panax notoginseng saponins on neuronal apoptosis in natural aging rats[J]. Tradit Chin Drug Res Clin Pharmacol, 2019, 30(10): 1159-1164. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY201910003.htm
    [21]
    巩子汉, 虎峻瑞, 段永强, 等. 白及多糖对胃溃疡模型大鼠血清IL-2R、IL-4及胃组织Caspase-8水平的影响[J]. 中国中医药信息杂志, 2019, 26(10): 35-39. doi: 10.3969/j.issn.1005-5304.2019.10.009

    GONG ZH, HU JR, DUAN YQ, et al. Effects of Bletilla baicalensis polysaccharide on serum IL-2R, IL-4 and caspase-8 expression in stomach tissue of model rats with gastric ulcer[J]. Chin J Inf Tradit Chin Med, 2019, 26(10): 35-39. doi: 10.3969/j.issn.1005-5304.2019.10.009
    [22]
    巩子汉, 王强, 段永强, 等. 白及多糖对胃溃疡模型大鼠胃组织TNF-α、IL-1β、IL-6及JNK、p38 MAPK基因蛋白表达水平的影响[J]. 中药药理与临床, 2019, 35(4): 90-95. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYL201904019.htm

    GONG ZH, WANG Q, DUAN YQ, et al. Effects of Bletilla baicalensis polysaccharide on the expression levels of TNF-α, IL-1β, IL-6, JNK and p38 MAPK genes in gastric tissues of rats with gastric ulcer[J]. Pharmacol Clin Chin Mater Med, 2019, 35(4): 90-95. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYL201904019.htm
    [23]
    MARTINON F, BURNS K, TSCHOPP J. The inflammasome: A molecular platform triggering activation of inflammatory caspases and processing of proIL-beta[J]. Mol Cell, 2002, 10(2): 417-426. doi: 10.1016/S1097-2765(02)00599-3
    [24]
    刘琦, 周星, 周联, 等. NLRP3炎症小体在慢性疾病中的研究进展[J]. 转化医学电子杂志, 2017, 4(3): 66-72. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHDZ201703018.htm

    LIU Q, ZHOU X, ZHOU L, et al. Research progress of the NLRP3 inflammasome in the pathogenesis of related chronic disease[J]. E J Transl Med, 2017, 4(3): 66-72. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHDZ201703018.htm
    [25]
    孙艺蕾, 钟镝, 李国忠. NLRP3炎性小体在缺血性脑卒中中的作用及研究进展[J]. 国际免疫学杂志, 2018, 41(1): 60-63. doi: 10.3760/cma.j.issn.1673-4394.2018.01.013

    SUN YL, ZHONG D, LI GZ. Research progress about the role of NLRP3 in ischemic stroke[J]. Int J Immunol, 2018, 41(1): 60-63. doi: 10.3760/cma.j.issn.1673-4394.2018.01.013
    [26]
    PLATNICH JM, MURUVE DA. NOD-like receptors and inflammasomes: A review of their canonical and non-canonical signaling pathways[J]. Arch Biochem Biophys, 2019, 670: 4-14. doi: 10.1016/j.abb.2019.02.008
    [27]
    NADATANI Y, HUO XF, ZHANG X, et al. NOD-like receptor protein 3 inflammasome priming and activation in barrett's epithelial cells[J]. Cell Mol Gastroenterol Hepatol, 2016, 2(4): 439-453. doi: 10.1016/j.jcmgh.2016.03.006
    [28]
    SPECHLER SJ, SOUZA RF. Barrett's esophagus[J]. N Engl J Med, 2014, 371(9): 836-845. doi: 10.1056/NEJMra1314704
    [29]
    YIN XL, WU HM, ZHANG BH, et al. Tojapride prevents CaSR-mediated NLRP3 inflammasome activation in oesophageal epithelium irritated by acidic bile salts[J]. J Cell Mol Med, 2020, 24(2): 1208-1219. doi: 10.1111/jcmm.14631
    [30]
    贺海峰, 华鹏, 崔翔. 大黄牡丹汤对脂多糖诱导肠上皮细胞焦亡及NLRP3/caspase-1通路的影响[J]. 河北医学, 2021, 27(11): 1791-1795. doi: 10.3969/j.issn.1006-6233.2021.11.06

    HE HF, HUA P, CUI X. Effect of Dahuang Mudan Decoction on lipopolysaccharide-induced pyrosis of intestinal epithelial cells and NLRP3/caspase-1 pathway[J]. Hebei Med, 2021, 27(11): 1791-1795. doi: 10.3969/j.issn.1006-6233.2021.11.06
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