Volume 37 Issue 5
Sep.  2021
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2021  >  37(5): 744-749
DONG Hui-min, XU Ru-ling, CHEN Xiao-qing, YANG Fu-yan, FAN Jun-ting, JU Jian-ming. UPLC-ESI-Q-TOF-MS Analysis of Xanthones in Swertia Chirayita[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(5): 744-749. doi: 10.14148/j.issn.1672-0482.2021.0744
Citation: DONG Hui-min, XU Ru-ling, CHEN Xiao-qing, YANG Fu-yan, FAN Jun-ting, JU Jian-ming. UPLC-ESI-Q-TOF-MS Analysis of Xanthones in Swertia Chirayita[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(5): 744-749. doi: 10.14148/j.issn.1672-0482.2021.0744
shu

UPLC-ESI-Q-TOF-MS Analysis of Xanthones in Swertia Chirayita

doi: 10.14148/j.issn.1672-0482.2021.0744
  • 1.

    The Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China

  • 2.

    Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

  • 3.

    School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China

  • Received Date: 2020-11-02
    Available Online: 2021-12-21
  • Publish Date: 2021-09-10
    Abstract
  • OBJECTIVE   To establish a qualitative analysis method of xanthones in Swertia chirayita by ultra-high performance liquid chromatography-time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS).METHODS   Acquity HSS T3 column (100 mm×2.1 mm, 1.8 μm) was used as the chromatographic column, with 0.1% formic acid in water-0.1% formic acid in acetonitrile as the mobile phase for gradient elution.The volume flow rate was 0.4 mL·min-1 and the column temperature was 40 ℃.In the positive and negative ion modes, the mass spectrum data of Swertia chirayita was collected and identified by Masslynx4.1 software combined with SciFinder database, comparison with mass spectrometry data analysis, reference materials and reference to relevant literatures.RESULTS   A total of 25 xanthone compounds were identified, 3 of which were first discovered including 7-O-β-D-xylopyranosyl-1, 8-dihydroxy-3-methoxyxanthone, swerpunilactone B and 8-O-[β-D-xylopyranose(1→6)-β-D-glucopyranose]-1, 7-dihydroxy-3-methoxyxanthone.CONCLUSION   The method can provide an experimental basis for the qualitative analysis of xanthones and the quality control of medicinal materials in Swertia chirayita.

     

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