Potential Mechanism of <i>Platycodon Grandiflorum</i> in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics

ZHOU Yang; XIE Tong; SHI Chen; KANG An; DENG Hai-shan; SHAN Jin-jun

doi:10.14148/j.issn.1672-0482.2021.0737

Volume 37 Issue 5

Sep. 2021

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Article Navigation > Journal of Nanjing University of traditional Chinese Medicine > 2021 > 37(5): 737-743

ZHOU Yang, XIE Tong, SHI Chen, KANG An, DENG Hai-shan, SHAN Jin-jun. Potential Mechanism of Platycodon Grandiflorum in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(5): 737-743. doi: 10.14148/j.issn.1672-0482.2021.0737

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ZHOU Yang, XIE Tong, SHI Chen, KANG An, DENG Hai-shan, SHAN Jin-jun. Potential Mechanism of Platycodon Grandiflorum in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(5): 737-743. doi: 10.14148/j.issn.1672-0482.2021.0737

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Potential Mechanism of Platycodon Grandiflorum in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics

doi: 10.14148/j.issn.1672-0482.2021.0737

ZHOU Yang^{1, 2
,},
XIE Tong¹,
SHI Chen¹,
KANG An²,
DENG Hai-shan^{1, 2
,
,},
SHAN Jin-jun^{1
,
,}

1.
Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

Received Date: 2021-07-05
Available Online: 2021-12-21
Publish Date: 2021-09-10

Abstract

Abstract

OBJECTIVE To investigate the potential mechanism of platycodon grandiflorum in preventing acute lung injury in mice based on non-target serum metabolomics.METHODS Twenty-four C57BL/6J mice were randomly divided into control, model, positive and platycodon grandiflorum groups. After 7 days of drug intervention, except for the control group, the other mice were injected with lipopolysaccharide (3 mg·kg^-1) to establish the acute lung injury model and the lung pathology and the expression of inflammatory factors in each group were determined. The serum of mice was collected, and the serum samples were detected by mass spectrometry using GC-MS. The serum metabolic profiles were compared by PCA and Metaboanalyst 5.0 was used to analyze related metabolic pathways.RESULTS Compared with the control group, the lung pathology of the model group was serious, and the inflammatory factors significantly increased (P < 0.05). Compared with the model group, the pathological conditions of the positive and platycodon grandiflorum groups were improved to some extent, and the inflammatory factors were significantly reduced (P < 0.01). Serum metabolomics results showed that the metabolic profile of the control group and the model group was significantly different, and the metabolic profiles of the positive and platycodon grandiflorum group were close to the normal group.CONCLUSION Platycodon grandiflorum can improve the inflammation in acute lung injury mice, its potential mechanism may be related to the callback variety of the content of serum metabolites and control multiple metabolic pathways.
- platycodon grandiflorum,
- acute lung injury,
- metabolomics,
- metabolic pathway

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[1]	陈朝阳, 姚茹, 王璐, 等. 莲心碱对LPS诱导小鼠急性肺损伤的保护作用[J]. 中国实验动物学报, 2018, 26(3): 343-348. doi: 10.3969/j.issn.1005-4847.2018.03.012
[2]	DING Z, ZHONG R, YANG Y, et al. Systems pharmacology reveals the mechanism of activity of Ge-Gen-Qin-Lian decoction against LPS-induced acute lung injury: A novel strategy for exploring active components and effective mechanism of TCM formulae[J]. Pharmacol Res, 2020, 156: 104759. doi: 10.1016/j.phrs.2020.104759
[3]	LI Y, HUANG J, FOLEY NM, et al. B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration[J]. Sci Rep, 2016, 6: 31284. doi: 10.1038/srep31284
[4]	杨瑞, 钱文娟, 彭琳秀, 等. 基于UHPLC-Q-exactive orbitrap/MS的桔梗汤治疗小鼠急性肺损伤的磷脂组学研究[J]. 药学学报, 2019, 54(1): 144-150. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB201901023.htm
[5]	CHU H, ZHANG A, HAN Y, et al. Metabolomics approach to explore the effects of Kai-Xin-San on Alzheimer's disease using UPLC/ESI-Q-TOF mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2016, 1015/1016: 50-61. doi: 10.1016/j.jchromb.2016.02.007
[6]	玄静, 刘燕, 张永清. 基于UPLC-QE-Orbitrap-MS的生姜干预腹泻小鼠血清代谢组学分析[J/OL]. 中国实验方剂学杂志, 2021: 1-12. https://doi.org/10.13422/j.cnki.syfjx.20211650.
[7]	孙桂江, 姜埃利, 李遇伯, 等. 代谢组学在疾病诊断及中药治疗的研究进展[J]. 中国中西医结合杂志, 2021, 41(1): 122-125. https://www.cnki.com.cn/Article/CJFDTOTAL-ZZXJ202101030.htm
[8]	WISHART DS. Emerging applications of metabolomics in drug discovery and precision medicine[J]. Nat Rev Drug Discov, 2016, 15(7): 473-484. doi: 10.1038/nrd.2016.32
[9]	MA XS, ZHANG YC, JIANG D, et al. Protective effects of functional amino acids on apoptosis, inflammatory response, and pulmonary fibrosis in lipopolysaccharide-challenged mice[J]. J Agric Food Chem, 2019, 67(17): 4915-4922. doi: 10.1021/acs.jafc.9b00942
[10]	INOUE S, IKEDA H. Differences in plasma amino acid levels in patients with and without bacterial infection during the early stage of acute exacerbation of COPD[J]. Int J Chron Obstruct Pulmon Dis, 2019, 14: 575-583. doi: 10.2147/COPD.S188422
[11]	TAO J, NIE Y, HOU Y, et al. Chemomics-integrated proteomics analysis of Jie-Geng-Tang to ameliorate lipopolysaccharide-induced acute lung injury in mice[J]. Evid Based Complement Alternat Med, 2016, 2016: 7379146. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992511/pdf/ECAM2016-7379146.pdf
[12]	钱文娟. "银翘"药对治疗H1N1肺炎的肺-肠轴相关代谢组学研究[D]. 南京: 南京中医药大学, 2019.
[13]	CHEN Y, DONG H, THOMPSON DC, et al. Glutathione defense mechanism in liver injury: Insights from animal models[J]. Food Chem Toxicol, 2013, 60: 38-44. doi: 10.1016/j.fct.2013.07.008
[14]	ZHANG YC, MA XS, JIANG D, et al. Glycine attenuates lipopolysaccharide-induced acute lung injury by regulating NLRP3 inflammasome and NRF₂ signaling[J]. Nutrients, 2020, 12(3): 611. doi: 10.3390/nu12030611
[15]	CHEN J, JIN Y, YANG Y, et al. Epithelial dysfunction in lung diseases: Effects of amino acids and potential mechanisms[J]. Adv Exp Med Biol, 2020, 1265: 57-70. doi: 10.1007/978-3-030-45328-2_4
[16]	ALLEN KD, MILLER DV, RAUCH BJ, et al. Homocysteine is biosynthesized from aspartate semialdehyde and hydrogen sulfide in methanogenic archaea[J]. Biochemistry, 2015, 54(20): 3129-3132. doi: 10.1021/acs.biochem.5b00118
[17]	RABINOVICH S, ADLER L, YIZHAK K, et al. Diversion of aspartate in ASS₁-deficient tumours fosters de novo pyrimidine synthesis[J]. Nature, 2015, 527(7578): 379-383. http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=111069781&site=ehost-live
[18]	ARSENIAN M. Potential cardiovascular applications of glutamate, aspartate, and other amino acids[J]. Clin Cardiol, 1998, 21(9): 620-624. http://www.onacademic.com/detail/journal_1000034877324210_4291.html

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Potential Mechanism of Platycodon Grandiflorum in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics

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Potential Mechanism of Platycodon Grandiflorum in the Prevention of Acute Lung Injury Based on Non-Target Serum Metabolomics

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