Volume 37 Issue 3
May  2021
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Article Navigation >  Journal of Nanjing University of traditional Chinese Medicine  > 2021  >  37(3): 428-433
SHI Jian-feng, PENG Zi-xuan, PENG Yun-ru, YAO Xiao-ming, CHEN Yun. Studies on the Metabolites of Caudatin-3-O-β-D-Cymaropyranoside in Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(3): 428-433. doi: 10.14148/j.issn.1672-0482.2021.0428
Citation: SHI Jian-feng, PENG Zi-xuan, PENG Yun-ru, YAO Xiao-ming, CHEN Yun. Studies on the Metabolites of Caudatin-3-O-β-D-Cymaropyranoside in Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(3): 428-433. doi: 10.14148/j.issn.1672-0482.2021.0428
shu

Studies on the Metabolites of Caudatin-3-O-β-D-Cymaropyranoside in Rats

doi: 10.14148/j.issn.1672-0482.2021.0428
  • 1.

    Department of Immunology, Nanjing Medical University, Nanjing, 211166, China

  • 2.

    Department of Laboratory, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China

  • 3.

    The Third Clinical Medical College, Xinjiang Medical University, Urumqi, 830011, China

  • 4.

    Department of Pharmacology and Toxicology, Jiangsu Provincial Institute of Traditional Chinese Medicine, Nanjing, 210028, China

  • Received Date: 2020-12-05
    Abstract
  •   OBJECTIVE   To study the rats' metabolism of caudatin-3-O-β-D-cymarose glycosides, a kind of sterioside in Radix Cynanchi Auriculati.   METHODS   Rats were given the a single dose of caudatin-3-O-β-D-cymarose glycosides by intragastric administration (20 mg/kg). Then the rats' plasma, urine, feces, and bile during the following 24 hours were collected. In addition, the processed samples were analyzed by UPLC-Q-TOF with MetabolynxTM software for the preliminary measurement of the metabolites of caudatin-3-O-β-D-cymarose glycosides in rats' plasma, urine, feces, and bile.   RESULTS   The original, hydrolytic and demethylated metabolites of caudatin-3-O-β-D-cymarose glycosides were found in plasma, urine, feces, and bile samples, specifically, the original caudatin-3-O-β-D-cymarose glycosides in urine, hydrolytic metabolites in plasma and bile, two methylated metabolites in feces, along with one methylated and three demethylated metabolites in bile.   CONCLUSION   The metabolism of caudatin-3-O-β-D-cymarose glycosides in rats is extensive, and the main types of metabolism are hydrolysis and demethylation.

     

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