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2024 Vol. 40, No. 4

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A Brief Discussion on the Clinical Value of Treatise on Cold Damage
MA Junjie, ZHOU Chunxiang
2024, 40(4): 329-333. doi: 10.14148/j.issn.1672-0482.2024.0329
[Abstract](226) [FullText HTML] (35) [PDF 1039KB](26)
Abstract:
There are many researchers on the clinical thinking of Treatise on Cold Damage, and a relatively complete research system on Treatise on Cold Damage has gradually been formed. However, there are still shortcomings, such as incomplete understanding of the clinical value of this book, the diagnosis and treatment of epidemic diseases focusing on febrile diseases and underestimating typhoid fever, problems in how classical theories deal with contemporary diseases and the application of classical prescriptions. This article proposes that Treatise on Cold Damage is the law for all diseases, treating typhoid fever as well as miscellaneous diseases; the diagnosis and treatment of epidemic diseases emphasizes the treatment of febrile disease and underestimates typhoid fever, but in fact, cold and warmth are closely related; classical research can learn from the thinking of western medicine, and the treatment of diseases must be based on Chinese medicine. At present, the application of classic prescriptions focuses on prescriptions and neglects theory and methods, and there is an urgent need to build a standardized system to provide ideas for clinical research on Chinese medicine classics.
The Influence of "Five Movements and Six Qi" Theory on Liu Wansu's Medical Theory System
LIU Liwei, REN Jiang, WANG Yitong, LIU Changhua
2024, 40(4): 334-340. doi: 10.14148/j.issn.1672-0482.2024.0334
[Abstract](97) [FullText HTML] (13) [PDF 1056KB](15)
Abstract:
The five movements and six qi theory (hereinafter referred to as “movement qi theory”) runs through Liu Wansu’s medical theoretical system, which is mainly reflected in its influence on the theory of pathogenesis, the creation of fiery heat theory, and the influence of Liu Wansu’s clinical diagnosis and treatment thoughts. The influence of movement qi theory on Liu Wansu’s pathogenesis theory includes three aspects: six qi alternation on pathogenesis, five movement hyperactivities on pathogenesis, and the root and branch of movement qi on pathogenesis. Liu’s understanding of the pathogenesis of six qi and the philosophical thought of “water is good and fire is evil” mainly influenced the creation of Liu Wansu’s “fire theory”. The influence of movement qi theory on Liu Wansu’s clinical diagnosis and treatment thoughts is mainly reflected in the classification of viscera, meridian and collateral diseases based on the theory of root and branch of middle qi, the diagnosis of pulses based on movement qi theory, the deduction of disease transmission and change using movement qi thinking, and the preparation of medicines based on qi. Liu Wansu initiated the innovation and debate of medical theory in the Jin and Yuan Dynasties, and had a profound impact on the rise of warm febrile diseases in the Ming and Qing Dynasties.
Study on the Anti-Liver Fibrosis Mechanism of Atractylenolide Ⅲ Regulating ASCT2-Mediated Mitochondria-Lysosome Interaction to Induce Hepatic Stellate Cell Senescence
FU Qiuyu, WANG Feixia, ZHANG Feng, ZHENG Shizhong, FU Jinbai
2024, 40(4): 341-349. doi: 10.14148/j.issn.1672-0482.2024.0341
[Abstract](74) [FullText HTML] (9) [PDF 3047KB](18)
Abstract:
  OBJECTIVE  To explore the anti-liver fibrosis effect and mechanism of Atractylenolide Ⅲ-induced hepatic stellate cell (HSC) senescence.  METHODS  ASCT2 siRNA and Atractylenolide Ⅲ (40 μmol·L-1) acted on human hepatic stellate cells LX2 respectively to inhibit ASCT2, MTT was used to evaluate cell viability, EdU method was used to detect cell proliferation, and senescence associated-β-galactosidase (SA-β-Gal) staining was used to detect cell senescence; Western blot was used to detect changes in the LC3-Ⅱ/Ⅰ ratio in LX2 cells, laser confocal detection was used to detect changes in LC3 autophagy flow and error protein accumulation, and the fluorescence of the lysosomal marker LAMP1 was also observed to detect lysosomal function and quantity; kits were applied to detect ROS and MDA levels as well as SOD activity in LX2 cells, and flow cytometry was used to analyze mitochondrial ROS levels and membrane potential. A CCl4-induced mouse liver fibrosis model was constructed. Atractylenolide Ⅲ was administered at 20, 30, or 40 mg·kg-1. HE, Masson, and Sirius Red staining were used to observe liver tissue damage and collagen deposition. Western blot was used to detect the expression levels of P21 and P16 in mice in each group, and SA-β-Gal staining and immunohistochemistry were used to analyze the situation and origin of senescent cells.  RESULTS  After inhibiting ASCT2, the viability of LX2 cells decreased and senescence increased (P < 0.01). Meanwhile, the autophagy function was enhanced and the number of lysosomes was increased but the function was weakened. After adding chloroquine (CQ) to clear lysosomes, the cell viability and autophagy function increased(P < 0.01). After inhibiting ASCT2, the levels of MDA and ROS in LX2 cells increased, and the activity of SOD decreased (P < 0.01). Among them, the level of mitochondrial ROS increased and the membrane potential decreased (P < 0.01). After adding rotenone, the cellular redox homeostasis was improved, and the number of lysosomes was restored (P < 0.01). In vivo experimental results showed that compared with the model group, Atractylenolide Ⅲ improved liver tissue structural damage and collagen deposition, induced HSC senescence in liver tissue of mice with liver fibrosis, and inhibited HSC activation marker α-smooth muscle actin (α-SMA), promoted the expression of senescence indicators P16 and P21 (P < 0.01).  CONCLUSION  Atractylenolide Ⅲ induces an increase in mitochondrial ROS and a decrease in membrane potential by inhibiting ASCT2, which further promotes the enhancement of HSC autophagy function, increases the number of lysosomes and weakens their function, thereby inducing the senescence of activated HSCs.
Syringic Acid Improves Cholestatic Liver Disease by Regulating Bile Acid Metabolism and Intestinal Barrier
LUO Xin, CHENG Peng, LU Yin, WEI Zhonghong
2024, 40(4): 350-358. doi: 10.14148/j.issn.1672-0482.2024.0350
[Abstract](65) [FullText HTML] (12) [PDF 3499KB](6)
Abstract:
  OBJECTIVE  To explore the regulatory effect of syringic acid in cholestatic mice based on bile acid metabolism and intestinal barrier.  METHODS  Twenty mice were randomly divided into control group, model group and low and high dose of syringic acid (70, 140 mg·kg-1) groups. Intrahepatic cholestasis was induced by intraperitoneal injection of α-naphthalene isothiocyanate after 2 h of administration on the fifth day. After the last dose, the changes of body weight and liver mass of mice were recorded. Liver function indexes in serum and histopathology were detected, qPCR verified the expression of tight junction proteins Zonula Occludens Protein 1 (ZO-1), Occludin and Claudin-5 in mouse colon tissues, the changes of metabolites in serum were analyzed by using nontargeted metabolomics, and the changes of total bile acids in liver and feces were detected.  RESULTS  Syringic acid could significantly reduce the serum alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) activity, total bilirubin (TBIL) and direct bilirubin (DBIL) levels (P < 0.05, P < 0.01) in the model group mice, and reduce liver damage and necrosis. Syringic acid reduced lymphocyte infiltration in the colon of mice in the model group and restored crypt morphology, while the high-dose syringic acid group significantly increased the mRNA expression levels of ZO-1, Occludin, and Claudin-5 in the colon (P < 0.05). The high-dose intervention of syringic acid significantly upregulated 11 metabolites and 29 metabolites, and the metabolites mainly involved the biosynthesis of secondary metabolites, secondary bile acid biosynthesis and bile secretion pathways. Syringic acid reduced the content of total bile acids in the liver and increased the excretion of total bile acids in feces (P < 0.05, P < 0.01).  CONCLUSION  Syringic acid can significantly improve the phenotype of cholestasis in cholestatic mice, improve the damage of intestinal barrier, and promote the metabolism of bile acids in cholestatic mice, which may be the key pathway for syringic acid to improve cholestasis.
Lipidomics Study of Huashi Baidu Granules in the Treatment of Respiratory Syncytial Virus Pneumonia Model Mice
YANG Bin, TANG Yu, SHI Chen, SHAN Jinjun
2024, 40(4): 359-368. doi: 10.14148/j.issn.1672-0482.2024.0359
[Abstract](66) [FullText HTML] (7) [PDF 8076KB](11)
Abstract:
  OBJECTIVE  To investigate the therapeutic effect of Huashi Baidu Granules on respiratory syncytial virus (RSV) pneumonia model mice and its effect on lung lipid metabolism.  METHODS  BALB/c mice were randomly divided into control group, model group, Ribavirin group, Dexamethasone group, low dose group of Huashi Baidu Granules and high dose group of Huashi Baidu Granules. Lung tissue samples of mice in each group were collected to observe pathological changes and detect mRNA expression levels of RSV-F, RSV-G, IL-1β, IL-6 and TNF-α genes. The lipid components of lung tissue were extracted and analyzed by ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high-resolution mass spectrometry (UPLC-Q Exactive Orbitrap MS) to find the changes in lipid metabolism.  RESULTS  The model group exhibited pulmonary interstitial thickening and inflammatory infiltration compared to the control group, the mRNA levels of viral load and inflammatory factors in lung tissue such as IL-1β and TNF-α were significantly increased (P < 0.001). Moreover, the model group exhibited significant abnormalities in lipid metabolism, including disorders in phosphatidylserine (PS), phosphatidylglycerol (PG), sphingomyelin (SM), ceramide (Cer), triglyceride (TG), diglyceride (DG), fatty acid (FA), ether phosphatidylcholine (PC O), ether lysophosphatidylcholine (LPC O). However, after the treatment of Huashi Baidu Granules (25.12 g·kg-1·d-1), the above indexes showed a significant improvement.  CONCLUSION  Huashi Baidu Granules can treat RSV pneumonia by regulating lipid metabolism, reducing viral load and alleviating inflammatory response in lung tissue.
Effect of Buyang Huanwu Decoction on Delaying Vascular Aging Based on miR-665/DRAM1 Signaling-Mediated Autophagy
YE Caibo, CHEN Xiangyu, DU Jieyong, YANG Yubin, SHU Zunpeng, ZHANG Li
2024, 40(4): 369-378. doi: 10.14148/j.issn.1672-0482.2024.0369
[Abstract](55) [FullText HTML] (4) [PDF 4818KB](10)
Abstract:
  OBJECTIVE  This study aimed to investigate the effects of Buyang Huanwu Decoction (BYHWD) on delaying vascular aging and explore whether the underlying mechanism is associated with microRNA-665 (miR-665)/DNA damage-regulated autophagy modulator1 (DRAM1)-mediated autophagy.  METHODS  Male SD rats with natural aging were randomly divided into the aging group, BYHWD low, medium, high dosage groups (9.25, 18.5, 37.0 g·kg-1) and resveratrol group (80 mg·kg-1), with a young group set as well. The rats in each group were dissected and the blood vessels were collected. ELISA was used to assess senescence associated β-galactosidase (SA-β-Gal) activity and advanced glycation end products (AGEs) level in vascular tissues. HE, Masson, and EVG staining were performed to observe the morphological structure of the vascular tissues. The qPCR was performed to detect the expression of miR-665 in vascular tissues. Bioinformatics analysis and dual-luciferase reporter gene experiments were used to validate the targeting relationship between miR-665 and DRAM1. Transmission electron microscope was used to observe the autophagosome. Western blot was performed to determine the protein expression of p16, DRAM1 and autophagy-related proteins Beclin1, p62 and LC3. Immunohistochemistry was used to detect the protein expression of DRAM1 in vascular tissues.  RESULTS  Compared to the young group, the aging group showed increased SA-β-Gal activity, AGEs level and p16 protein expression (P < 0.01), disordered arrangement of vascular tissues, thickened media, increased collagen fibers, fractured and disorganized elastic fibers. The expression of miR-665 was upregulated (P < 0.01). The number of autophagosomes was reduced. The protein expression of Beclin1 and LC3Ⅱ/Ⅰ downregulated (P < 0.01), while the protein expression of p62 was upregulated (P < 0.01). In addition, the protein expression of DRAM1 was downregulated in vascular tissues (P < 0.01). Compared to the aging group, intervention with BYHWD and resveratrol reduced SA-β-Gal activity (P < 0.01), AGEs level and p16 protein expression (P < 0.05, P < 0.01), improved vascular morphology and elastic fiber structure, reduced collagen fibers. High dose BYHWD significantly downregulated miR-665 expression (P < 0.01), increased the number of autophagosomes. Medium and high dose of BYHWD significantly upregulated protein expression of Beclin1 and LC3Ⅱ/Ⅰ (P < 0.01), downregulated protein expression of p62 (P < 0.05, P < 0.01). High dose BYHWD significantly upregulated protein expression of DRAM1 in vascular tissues of aging rats (P < 0.05). Bioinformatics analysis revealed the presence of specific complementary binding sites between the sequences of miR-665 and DRAM1. Dual-luciferase reporter assays confirmed that miR-665 targeted DRAM1 gene and negatively regulated DRAM1 protein expression.  CONCLUSION  BYHWD may promote the protein expression of DRAM1 by inhibiting the expression of miR-665, thereby promoting vascular autophagy and delaying vascular aging.
Study on the Enzymatic Deproteinization Technology, Composition Analysis and Immunomodulatory Activity of Isatidis Radix Polysaccharides
LAI Mengting, Memitimin Metsawur, LI Tong, XIAO Ping, SU Shulan, DUAN Jinao
2024, 40(4): 379-390. doi: 10.14148/j.issn.1672-0482.2024.0379
[Abstract](61) [FullText HTML] (7) [PDF 3849KB](7)
Abstract:
  OBJECTIVE  To optimize the deproteinization process of Isatidis Radix polysaccharides and further explore its immunomodulatory activity, and to provide a scientific basis for the development and utilization of it.  METHODS  The optimum conditions of enzymatic deproteinization were optimized by a single factor combined with the Box-Behnken response surface method. The chemical composition and structural characteristics of deproteinized Isatidis Radix polysaccharides were analyzed by UV-visible spectrum, Fourier transform-infrared spectroscopy, high-performance gel permeation chromatography, high-performance liquid chromatography and scanning electron microscopy. The effects of deproteinized Isatidis Radix Polysaccharide on neutrophils, macrophages, IL-1β and IL-6 in zebrafish were investigated by using a zebrafish immunocompromised model.  RESULTS  The optimal enzymatic deproteinization process was as follows: trypsin 500 U·mL-1, pH 8.0, enzymatic hydrolysis time 5 h, enzymatic hydrolysis temperature 37 ℃. The deproteinization rate was (86.39±0.07)%, and the comprehensive score was (91.15±0.37)%. Ultraviolet, infrared spectroscopy scanning and scanning electron microscopy showed that the protein contained in the crude polysaccharide could be removed by enzymatic method. The relative molecular weight of the polysaccharides were between 5.82 and 60.26 kDa. The monosaccharide mole composition was mannose∶ rhamnose∶galacturonic acid∶glucose∶galactose∶arabinose=2.17∶0.96∶2.90∶83.25∶4.88∶5.84. The results of immune activity evaluation showed that when the concentration of deproteinized Radix Isatidis polysaccharides was 50~300 μg·mL-1, it could significantly increase the density of zebrafish immune cells, increase the number of macrophages, and reduce the content of IL-1β and IL-6 in immunocompromised zebrafish, thus exerting immunomodulatory effects.  CONCLUAION  The enzymatic method can effectively remove the proteins contained in the crude polysaccharides of Isatidis Radix, and the deproteinized Isatidis Radix polysaccharides have certain immunomodulatory effects.
Preparation of Sinomenine Microemulsion by D-Optimal Mixture Design and Evaluation of Its Pharmacodynamics
GAO Qin, CHEN Nan, JIA Letong, WANG Jie, CHEN Jing, YANG Yuwei, LYU Zhiyang
2024, 40(4): 391-398. doi: 10.14148/j.issn.1672-0482.2024.0391
[Abstract](51) [FullText HTML] (6) [PDF 2035KB](4)
Abstract:
  OBJECTIVE  To optimize the preparation process of sinomenine microemulsion and evaluate its pharmacodynamics.  METHODS  HPLC method for sinomenine content determination was established, and sinomenine microemulsion prescription was initially screened by solubility test and pseudo-ternary phase diagram. D-optimal mixing experimental design method was used to optimize sinomenine microemulsion prescription with particle size and drug load as investigation indexes, and its particle size, drug load and stability were evaluated. Transdermal absorption was investigated by transdermal test in vitro, and the anti-inflammatory effect was evaluated by ear swelling test.  RESULTS  With methanol:0.1% phosphoric acid (40∶60) as the mobile phase, the detection wavelength was 262 nm, and the method was suitable for the determination of sinomenine. The optimal formula of microemulsion was obtained as castor oil (7.0%), PEG40 hydrogenated castor oil/anhydrous ethanol (69.0%), the optimal Km value was 3∶1, and distilled water (24.0%). The average particle size of the microemulsion was 18.76 nm, the PDI was 0.072 and the drug loading was 5.225%. The cumulative permeability of 1.0% sinomenine microemulsion at 12 h was 1.223 4 μg·cm-2, and the steady permeability rate was 0.0649 μg·cm-2·h-1, which was better than sinomenine solution. The inhibitory rate of sinomenine microemulsion was 65.07%, which was similar to dexamethasone.  CONCLUSION  The preparation of sinomenine microemulsion has the advantages of stable process, high drug loading, good transdermal absorption and anti-inflammatory effect, which can provide reference for the development of sinomenine transdermal drug delivery preparation.
Fingerprint and Identification of Chemical Constituents of the Extract of Fructus Ligustri Lucidi-Ecliptae Herba
GAO Yutong, SONG Xiuping, LI Nan, SHANG Yonglin, HAN Fei
2024, 40(4): 399-412. doi: 10.14148/j.issn.1672-0482.2024.0399
[Abstract](46) [FullText HTML] (7) [PDF 5313KB](3)
Abstract:
  OBJECTIVE  To establish high performance liquid chromatography (HPLC) fingerprints of the extract of Fructus Ligustri Lucidi-Ecliptae Herba and to identify the structure of its chemical constituents.  METHODS  The chromatographic separation was performed on ZORBAX Extend C18 (250 mm×4.6 mm, 5 μm) column with the mobile phase consisting of acetonitrile and 0.1% formic acid solution in gradient elution mode at a flow rate of 1.0 mL·min-1. The optimum column temperature was set at 35 ℃, the detection wavelength was 265 nm, and the injection volume was 10 μL. The HPLC fingerprints of 15 batches of herb pair from different origins were established. Then, similarity evaluation, cluster analysis, and orthogonal partial least squares discriminant analysis were carried out. The chemical structures of the constituents of the herb pair were identified based on the high-resolution mass spectrometry utilizing the UPLC-Q-Exactive Orbitrap MS in positive and negative-ion modes.  RESULTS  As a result, the similarity of the 15 batches of samples varied from 0.865 to 0.992. A total of 16 common peaks were identified in the fingerprints, and 7 of them were identified by comparision with the reference substances. Furthermore, 55 chemical compounds were detected, and 7 of them were accurately identified based on the reference substances, and the chemical structures of the rest 48 components were temporarily speculated.  CONCLUSION  The fingerprint method established in this study is simple, reliable, and reproducible, and can be further used for the quality control of Fructus Ligustri Lucidi-Ecliptae Herba herb pair. The developed UPLC-Q-Exactive Orbitrap MS method can be applied to identify the structures of chemical constituents in this herb pair and lay a foundation for further research.
Clinical Study on the Treatment of Advanced Liver Cancer of Qi Deficiency and Toxic Stasis Type by Jiawei Yupingfeng San
WANG Zongao, ZHANG Minghui, SUN Hua, OUYANG Yiran, ZHAO Lanmei, ZHANG Ting, YAO Fei, YUAN Qin, JIANG Guorong, ZHANG Lurong, LIU Min
2024, 40(4): 413-418. doi: 10.14148/j.issn.1672-0482.2024.0413
[Abstract](90) [FullText HTML] (10) [PDF 1075KB](22)
Abstract:
  OBJECTIVE  To observe the clinical efficacy and effect on serum thymic stromal lymphopoietin (TSLP) levels of patients with advanced liver cancer of qi deficiency and toxic stasis type by Jiawei Yupingfeng San.  METHODS  Using random double blind method, 120 patients with advanced liver cancer of qi deficiency and toxic stasis type were randomly divided into 3 groups: Jiawei Yupingfeng San group, Yupingfeng San group, and placebo group, each consisting of 40 cases. All patients in the 3 groups were given conventional treatment such as radiotherapy, chemotherapy, interventional or targeted therapy; Jiawei Yupingfeng San group was given Jiawei Yupingfeng San granules, Yupingfeng San group was given Yupingfeng San granules, and placebo group was given placebo. The course of treatment was 2 months. The changes of Karnofsky functional status score (KPS score), TCM syndrome score, tumor size and serum TSLP level in the 3 groups were observed before and after treatment, and the correlation between the changes of tumor size and TSLP was analyzed.  RESULTS  After treatment, the KPS scores of Yupingfeng San group and Jiawei Yupingfeng San group were significantly increased (P < 0.05, P < 0.01), TCM syndrome score were decreased (P < 0.01), tumor growth (P < 0.05, P < 0.01) was delayed, and serum TSLP levels (P < 0.05, P < 0.01) were decreased. Furthermore, there was a slight positive correlation between changes in tumor size and changes in TSLP (P < 0.05). In terms of improving tumor size, the curative effect of Jiawei Yupingfeng San group was better than that of Yupingfeng San group (P < 0.05). During the treatment period, no obvious adverse reactions were observed in the 3 groups of patients.  CONCLUSION  Combined with conventional treatment, Jiawei Yupingfeng San can significantly delay tumor growth in patients with advanced liver cancer of qi deficiency and toxic stasis type and improve patients' TCM syndromes and their quality of survival. The therapeutic mechanism is related to reducing the expression of serum TSLP and improving the immune status of patients, thereby delaying the growth of tumors.
Clinical Efficacy and Transcriptomic Study on the Treatment of Coronary Heart Disease Angina of Qi Deficiency and Blood Stasis Type with Maitong Jun'an Decoction
WANG Ziyang, LIU Meizhi, HU Xiaozhen, ZHOU Miao, WENG Jiahao, LAI Zhikun, SUN Yongning
2024, 40(4): 419-428. doi: 10.14148/j.issn.1672-0482.2024.0419
[Abstract](80) [FullText HTML] (11) [PDF 5403KB](19)
Abstract:
  OBJECTIVE  To observe the clinical efficacy of Maitong Jun'an Decoction in treating coronary heart disease (CHD) angina of qi deficiency and blood stasis type, and preliminarily elucidate its possible mechanism of action through transcriptomics methods.  METHODS  A total of 140 patients with CHD angina of qi deficiency and blood stasis type were included and randomly divided into a treatment group and a control group, with 70 cases in each group. During the treatment period, 3 patients in the control group dropped out. The control group received basic Western medicine treatment for secondary prevention of CHD, while the treatment group received Maitong Jun'an Decoction in addition to the treatment in the control group. The treatment period for both groups was 8 weeks. Before and after treatment, the patients in both groups were evaluated for the TCM syndrome score, Canadian Cardiovascular Society (CCS) angina grading, Seattle angina questionnaire (SAQ) score, self-rating anxiety scale (SAS), self-rating depression scale (SDS) score, and adverse reactions. The peripheral blood of 9 patients before and after treatment was selected for transcriptomic sequencing based on the principle of gender, age, and disease duration matching.  RESULTS  After treatment, the TCM syndrome scores and total scores of the 2 groups were significantly reduced(P < 0.01). The treatment group was better than the control group in improving chest pain, chest tightness, shortness of breath, fatigue and total score (P < 0.05, P < 0.01); the overall improvement rate of CCS angina grading in the treatment group was better than that in the control group (P < 0.05); the SAQ, SAS and SDS scores of the 2 groups were significantly reduced before and after treatment (P < 0.01), and the SAQ score of the treatment group was improved better than that of the control group (P < 0.05, P < 0.01). The transcriptomics results showed that there were 862 significantly different mRNAs before and after treatment, including 509 up-regulated and 353 down-regulated. GO analysis showed that there were 666 biological processes in the differentially expressed mRNAs, mainly including viral gene expression, translation initiation, RNA catabolism, etc. There were 112 cell components, mainly including focal adhesion, ribosome subunit, nuclear spot, etc. There were 94 molecular functions, mainly including double-stranded RNA binding, cadherin binding, transcription co-regulatory factor activity, etc. KEGG analysis showed that the differentially expressed mRNAs enriched in 20 signaling pathways, mainly including glycerophospholipid metabolism pathway, AMPK signaling pathway, ribosome pathway, etc.  CONCLUSION  Maitong Jun'an Decoction can improve clinical symptoms in patients with CHD angina of qi deficiency and blood stasis type. Its mechanism of action is multi-target and multi pathway, mainly related to the regulation of glycerophospholipid metabolism pathway, AMPK signaling pathway, ribosome pathway.
Summary of Literature Research and Utilization of Unknown Prescriptions
WU Chengyan
2024, 40(4): 429-434. doi: 10.14148/j.issn.1672-0482.2024.0429
[Abstract](121) [FullText HTML] (11) [PDF 1049KB](10)
Abstract:
Nameless prescriptions are an important component of traditional Chinese medicine literature, with a large number and wide distribution in various types of literature such as Chinese medical literature, excavated medical literature, stone inscriptions, Dunhuang Turpan medical literature, and overseas returned medical literature. The research on the literature organization of unnamed prescriptions involves multiple perspectives and levels of research, including the screening of the sources of unnamed prescriptions, the study of citation and loss, the study of unnamed prescription drugs, the study of disease names and treatment standards, the study of the evolution of unnamed and famous prescriptions, the study of dosage forms and usage, and the identification and application of authentic and fake literature. Organizing literature on anonymous prescriptions throughout history, tracing the academic development of anonymous prescriptions, and summarizing the characteristics and characteristics of anonymous prescriptions are of great significance for promoting research on prescription literature and further utilization of anonymous prescriptions.

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