南京中医药大学学报

Analysis of the Diagnosis and Treatment of Female Infertility in The Yellow Emperor's Inner Classic Based on Time Rhythm

ZHENG Lingxin, TAN Yong

2024, 40(2): 109-114. doi: 10.14148/j.issn.1672-0482.2024.0109

[Abstract](153) [FullText HTML] (17) [PDF 529KB](29)

Abstract:
Based on the theory of harmony between man and nature, this article systematically elaborates on the theoretical connotation of "adapt to the time" and emphasizes the significant role of daily rhythm, monthly rhythm, annual rhythm and super annual rhythm in female infertility. This paper proposes that female fertility and the implementation of assisted reproductive technology are influenced by time rhythms. It is recognized that irregular menstruation is the key to the onset of female infertility, and the treatment should focus on regulating menstrual cycle. This article further refines the diagnosis and treatment plan of traditional Chinese medicine for female infertility through methods such as hourly, weekly, seasonal and age adjustment, which provides ideas and methods for clinical diagnosis and treatment of female infertility and the enlightenment of its medication therapy.

Exploring the Pathogenesis of Type 2 Diabetes Mellitus Based on Trinity Life View of Physique-Qi-Spirit

LIU Feng, SU Bochao, ZHANG Wenchun

2024, 40(2): 115-119. doi: 10.14148/j.issn.1672-0482.2024.0115

[Abstract](74) [FullText HTML] (5) [PDF 1071KB](16)

Abstract:
To explore the pathogenesis of type 2 diabetes mellitus(T2DM) based on the trinity life view of physique-qi-spirit, and point out that the occurrence and development of T2DM are the three elements of life, i.e. physique, qi and spirit, which have pathological changes. This paper discusses from three aspects: physique, qi, and spirit. The changes in physique include structural changes, abnormal physical movements, and changes in tangible and subtle substances; the changes in qi include qi deficiency and abnormal qi transformation; the changes in spirit include carnal desire excess and emotional imbalance. This paper provides new ideas for the clinical diagnosis and treatment of T2DM.

Analysis of Professor Xia Guicheng's "Menstrual Circle Rhythm and Menstrual Circle Regulation Method" from the Perspective of Yang Heat Circle Movement

XU Jing, LIU Jian

2024, 40(2): 120-123. doi: 10.14148/j.issn.1672-0482.2024.0120

[Abstract](120) [FullText HTML] (5) [PDF 1033KB](21)

Abstract:
Professor Xia Guicheng, a master of traditional Chinese medicine, advocated the "menstrual circle rhythm and menstrual cycle regulation method", which is an inherent theory in traditional Chinese medicine that explains the laws of circular motion and biological clock, and helps to analyze the changes in menstrual cycle and reproductive rhythm. The circular motion is a universal law of the movement of life in the universe. The ancients described the circular motion of the fall, rise, sinking and floating of yang heat related to biological life in nature through the 24 solar terms. The menstrual cycle is the result of a woman's own movement of fall, rise, sinking and floating of yang heat, as well as being influenced by the natural movement of yang heat. There is a corresponding relationship between the menstrual circle rhythm and the circular movement of natural yang heat: yang heat rises to the extreme and then falls, corresponding to the fact that yang accumulates to the extreme and then transforms into yin during menstruation; the decrease of yang heat in the golden autumn corresponds to the disappearance of yang and the growth of yin in the later menstrual period; yang heat falls to the extreme and then rises, corresponding to the fact that yin accumulates to the extreme and then transforms into yang in the intermenstrual period; yang heat rises in spring corresponds to the disappearance of yin and the growth of yang in the premenstrual period. The essence of the menstrual cycle regulation method is to restore the normal rhythm of the body's growth and decline of yin and yang, as well as the rise and fall of movement and stillness based on the menstrual cycle.

Explore the Pathogenesis and Treatment of Chronic Obstructive Pulmonary Disease Based on the Kenang Theory

XU Mengjiao, GAO Feng

2024, 40(2): 124-128. doi: 10.14148/j.issn.1672-0482.2024.0124

[Abstract](77) [FullText HTML] (6) [PDF 1076KB](15)

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This paper expounds the connotation and origin of the Kenang theory, as well as its correlation with chronic obstructive pulmonary disease (COPD), and focuses on the pathogenesis and treatment of COPD based on the Kenang theory. The Kenang theory emphasizes the importance of the pathological products of phlegm and stasis, and holds that the deficiency of primordial qi and the disorder of qi activity can lead to the intermingling of phlegm and blood, which is highly similar to the etiology and pathogenesis of COPD. The treatment should be guided by the Kenang theory, eliminating phlegm and promoting blood circulation, and its basic treatment principles is to treat phlegm and blood stasis together. Meanwhile, the regulation of qi activity should be paid attention to help eliminate phlegm and promote blood circulation, and the spleen and kidney should be strengthened to nourish primordial qi and consolidate the root, providing new ideas for the clinical treatment of COPD.

The Effect of Bushen Zhuyun Decoction on Treg, Th17 and Their Related Factors in Luteal Phase Defect Infertility Rats

RUAN Fang, ZHOU Huifang

2024, 40(2): 129-136. doi: 10.14148/j.issn.1672-0482.2024.0129

[Abstract](92) [FullText HTML] (13) [PDF 3454KB](14)

Abstract:
OBJECTIVE To investigate the effects of Bushen Zhuyun Decoction (BSZYD) on regulatory T cells (Treg), helper T cell 17 (Th17) and related factors in rat with luteal phase defect (LPD) infertility. METHODS Rats were administered mifepristone mixture (10 mL·kg-1) to construct the LPD-induced infertility model. The positive drug dydrogesterone mixture (0.02 g·kg^-1) and low (4.5 g·kg^-1), medium (9 g·kg^-1), and high (18 g · kg^-1) doses of BSZYD were given for intervention. Uterine tissue morphology of the rat was evaluated by HE staining; the ratio of Treg and Th17 and related factor contents in rat peripheral blood were detected by flow cytometry and ELISA; the protein and mRNA expression levels of Treg and Th17 cell transcription factors were detected by Western blot and qPCR. RESULTS Compared with the blank group, the endometrial thickness of the model group significantly decreased (P < 0.01), the proportion of Treg and the content of the anti-inflammatory factor interleukin-10 (IL-10) reduced significantly (P < 0.01), the percentage of Th17 cells and the inflammatory factor interleukin-17A (IL-17A) were significantly increased (P < 0.01), the protein and mRNA expression levels of FOXP3 were inhibited (P < 0.05, P < 0.001), the RORγT protein and mRNA levels were higher (P < 0.01, P < 0.001). Compared with the model group, the dydrogesterone and middle- and high-dose BSZYD could increase endometrial thickness (P < 0.05, P < 0.01), promoting the proliferation and development of endometrial glands; the percentage of Treg cells and IL-10 content increased significantly (P < 0.01, P < 0.001), the percentage of Th17 cells decreased significantly (P < 0.01, P < 0.001), and FOXP3 protein expression was increased (P < 0.01, P < 0.001); IL-17A content and RORγT protein expression were decreased in the middle and high-dose BSZYD groups (P < 0.05, P < 0.01); the FOXP3 mRNA levels in the dydrogesterone group and the high-dose BSZYD group were significantly increased (P < 0.01, P < 0.001), and the expression of RORγT mRNA was down-regulated (P < 0.001). CONCLUSION BSZYD can regulate the immune balance of Treg and Th17 cells in LPD-infertility rats and improve endometrial receptivity.

Study on the Effect of Huangqi-Ezhu-Chonglou Combination on Proliferation and Migration of Colorectal Cancer Cells by Regulating Macrophage Polarization

DU Lili, WANG Gang, LIANG Yan, ZHAO Fan, YING Jiahui, YIN Gang, TANG Decai, BIAN Yong

2024, 40(2): 137-144. doi: 10.14148/j.issn.1672-0482.2024.0137

[Abstract](119) [FullText HTML] (14) [PDF 2152KB](10)

Abstract:
OBJECTIVE To investigate the effect of Huangqi-Ezhu-Chonglou combination on macrophage polarization and its mechanism of inhibiting colorectal cancer (CRC) cells proliferation and migration. METHODS THP-1 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and interleukin-4 (IL-4) to establish M2 macrophage polarization model. The experiment was divided into M0 group (PMA treatment), M2 group (PMA+IL-4 treatment), and M2+ Huangqi-Ezhu-Chonglou combination group (PMA+IL-4+Huangqi-Ezhu-Chonglou combination treatment).The effect of Huangqi-Ezhu-Chonglou combination freeze-dried powder on the viability of macrophage was detected by CCK-8 method. The expression of macrophage polarization markers, glutaminase (GLS) mRNA and protein was detected by qPCR and Western blot. The levels of interleukin-10 (IL-10), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) in cell supernatant were detected by ELISA. CCK-8 method and Transwell assays were used to detect the proliferation and migration of HCT116 cells intervened by the supernatant of macrophage culture treated with Huangqi-Ezhu-Chonglou combination, namely conditioned medium (CM). RESULTS Compared with the M0 group, the expression levels of IL-10, mannose receptor (CD206), arginase 1 (ARG1), and GLS mRNA and protein in the M2 group were significantly increased (P < 0.01, P < 0.001), the levels of IL-10 and TGF-β secreted by macrophages were significantly increased (P < 0.01, P < 0.001); compared with the M2 group, the M2+ Huangqi-Ezhu-Chonglou combination group had significantly reduced IL-10, CD206, ARG1, and GLS mRNA and protein expression (P < 0.05, P < 0.01), the mRNA and protein levels of TNF-α and inducible nitric oxide synthase (iNOS) were significantly increased (P < 0.05, P < 0.01, P < 0.001), the interleukin-1β (Interleukin-1β, IL-1β) mRNA expression significantly increased (P < 0. 01), and the contents of IL-10 and TGF-β in the cell supernatant significantly decreased (P < 0.05, P < 0.01), while TNF-α content significantly increased (P < 0.01). CCK-8 and Transwell results showed that compared with the M0-CM group, the M2-CM promoted the proliferation and migration of HCT116 cells (P < 0.01, P < 0.001), the M2+ Huangqi-Ezhu-Chonglou-CM group significantly inhibited HCT116 cell proliferation and reduced cell migration compared to the M2-CM group (P < 0.01, P < 0.001). CONCLUSION Huangqi-Ezhu-Chonglou combination can inhibit colorectal cancer cells proliferation and migration by regulating macrophage polarization, and its mechanism may be related to the changes in the expression of GLS, a key enzyme in glutamine metabolism.

Efficacy Evaluation of Qishen Yizhi Formula in Improving the Learning and Memory Ability of D-Galactose Induced Subacute Aging Mice

CHEN Yang, ZHU Ziqiang, LU Yunqing, ZHENG Jiani, CAO Cheng, TONG Jiaxiang, LI Xuan, GUO Sheng, KANG Hongjie, DUAN Jinao, ZHU Yue

2024, 40(2): 145-152. doi: 10.14148/j.issn.1672-0482.2024.0145

[Abstract](66) [FullText HTML] (7) [PDF 2383KB](3)

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OBJECTIVE To evaluate the effect of Qishen Yizhi formula on improving learning and memory ability in D-galactose subcutaneous injection induced subacute aging mice. METHODS Subacute aging mice model mice were developed by D-galactose subcutaneous injection and then treated with positive drug donepezil (2 mg·kg^-1·d^-1) and Qishen Yizhi formula water extracts in low (1.33 g·kg^-1·d^-1) and high dose group (2.67 g·kg^-1·d^-1). The learning and memory abilities of mice were evaluated using Morris water maze and Y maze tests; HE staining was used to examine hippocampal damage in model mice; TUNEL was used to detect apoptosis of mouse hippocampal tissue; ELISA was used to detect the expression levels of oxidative stress factors and inflammatory factors in the mouse hippocampus tissue; Western blot was used to detect the expression of signaling pathway proteins related to apoptosis, oxidative stress and inflammatory stress in the hippocampus of mice. RESULTS The water extract of Qishen Yizhi formula significantly shortened the latency and distance of model mice for reaching the platform in the water maze test (P < 0.01), and significantly increased the number of crossing the platform (P < 0.01); increased the exploration time and number of the Y maze new arm in model mice (P < 0.05); inhibited the TUNEL fluorescence expression in the hippocampus of model mice (P < 0.01); upregulated the activity of the oxidative stress factor superoxide dismutase (SOD) (P < 0.05) and glutathione (GSH) content (P < 0.05), and downregulated malondialdehyde (MDA) content (P < 0.05); reduced interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF-α) expression levels (P < 0.05, P < 0.01); decreased the expression of apoptosis signaling pathway proteins Cleaved Caspase-3 and Caspase-3 (P < 0.05), upregulated the expression of oxidative stress signaling pathway proteins Nrf2 and HO-1 (P < 0.05), and downregulated the expression of inflammatory stress signaling pathway proteins p-NF-κB and NF-κB (P < 0.05). CONCLUSION Qishen Yizhi formula can improve the learning and memory ability of subacute aging model mice injected with D-galactose, which may be related to its inhibitory effect on hippocampal oxidative stress and inflammatory stress.

Analysis of the Material Basis and Efficacy of the Differences in the Preparation of Pinellia Ternate before and after Concoction Based on UPLC-Q-TOF-MS/MS and Network Pharmacology

SHI Kunqun, XIONG Yue, QIAN Xiang, ZHU Yufeng, YAO Yi, ZHANG Qian, LIU Shijia

2024, 40(2): 153-166. doi: 10.14148/j.issn.1672-0482.2024.0153

[Abstract](137) [FullText HTML] (18) [PDF 6751KB](15)

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OBJECTIVE To screen and identify the differential substance bases of Pinellia ternate and its different concoctions, conduct network pharmacological analysis on the common and differential substance bases, and explore the relationship between the substance bases and the changes in the efficacy of Pinellia ternate before and after concoction based on the UPLC-Q-TOF-MS/MS and multivariate statistical analysis. METHODS The main substance bases of 42 batches of samples were examined by UPLC-Q-TOF-MS/MS, and the differential components were screened by orthogonal partial least squares discriminant analysis (OPLS-DA) with VIP>1.5, P < 0.01 and FC>2 or < 0.5 as the screening criteria. The targets were further retrieved from the TCMIP database, and their protein interactions were analysed by GO enrichment and KEGG enrichment to visualise the "herbal-component-target-pathway" map. RESULTS Compared with Pinellia ternate, Pinelliae Rhizoma Praeparatum has 14 different components, mainly glycyrrhetinic acid, glycyrrhizic acid and glycyrrhizin, etc. The components with reduced content were mainly amides. There were 18 differential constituents between raw and ginger, mainly nucleosides, flavonoids and amino acids. The content of guanosine, xanthine and tyrosine was reduced, while the content of adenosine monophosphate was increased. There were 18 differential components between raw and Pinelliae Rhizoma Praeparatum Cum Alumine, and the relative content of many components in Pinelliae Rhizoma Praeparatum Cum Alumine was reduced, such as sphingomyelin. Further, the TCMIP database was used to retrieve targets from the differential substance base, and protein interaction analysis was performed on the targets, resulting in 67 core targets for Pinellia ternate, 45 core targets for Pinelliae Rhizoma Praeparatum, and 38 core targets for Pinelliae Rhizoma Praeparatum cum Zingibere Et Alumine. Finally, the metabolic pathways were analyzed by GO enrichment and KEGG enrichment. CONCLUSION The UPLC-Q-TOF-MS/MS method established in this experiment can better isolate and identify the chemical components in Pinellia ternate. Combined with multivariate statistical analysis and network pharmacology, the material basis and potential mechanism of action of Pinellia ternate and its concoction products can provide ideas for the study of the action targets and provide data support for the rational clinical application of Pinellia ternate and its concoction products.

Study on the Multi-Component Skin Pharmacokinetics of Crossbow Microemulsion after Transdermal Administration Based on Microdialysis Combined with UPLC

XIE Huan, SONG Xuanfei, ZHOU Bin, LIU Yao, YANG Fangfang, CHEN Yinglong, ZHU Yan, ZHANG Yongping

2024, 40(2): 167-173. doi: 10.14148/j.issn.1672-0482.2024.0167

[Abstract](58) [FullText HTML] (9) [PDF 2083KB](2)

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OBJECTIVE To investigate the pharmacokinetic characteristics of crossbow microemulsion in rabbit skin. METHODS The concentrations of chlorogenic acid, benzoyl new aconitine, periplocin and new aconitine were determined by UPLC. The effects of temperature, perfusion speed and mass concentration on the recovery rate of the probe were investigated by incremental and decrement methods, the changes of multi-component content in the dialysate at different time points after transdermal administration of crossbow microemulsion were determined by UPLC, and DAS 2.0 software was used to process the data. RESULTS The recovery rate of the probe increased with increasing temperature, and the higher the flow rate, the lower the recovery rate. The pharmacokinetic parameters of chlorogenic acid, benzoyl new aconitine, periplocin and new aconitine in microemulsion C_max were (3.24±0.39) (1.04±0.29) (4.78 ± 2.15) (2.86 ± 0.55) μg·mL^-1, respectively, and in AUC_0-∞ were (52.42 ± 30.84) (7.70 ± 3.73) (42.23 ± 13.93) (22.68 ± 12.51) h·mg·mL^-1, respectively. CONCLUSION A real-time in vivo microdialysis sampling technique was first used and a UPLC detection technique was established to study the skin pharmacokinetics of multi-components of crossbow microemulsion after transdermal administration, which provides a reference for the follow-up investigation and application of crossbow microemulsion.

Study on the Medication Rule and Action Mechanism of Traditional Chinese Medicine Immune Enhancer Based on Data Mining and Network Pharmacology

XIN Liyuan, ZHENG Liang

2024, 40(2): 174-183. doi: 10.14148/j.issn.1672-0482.2024.0174

[Abstract](117) [FullText HTML] (6) [PDF 5628KB](21)

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OBJECTIVE To explore the compatibility rules of traditional Chinese medicines used to enhance immunity in compound patent prescriptions using data mining method, and analyze the potential mechanism of action of core drugs by network pharmacology and molecular docking technology. METHODS The patent data of the immunity enhancing compound of traditional Chinese medicine from the State Intellectual Property Office was mined, the frequency of traditional Chinese medicine, association rules and complex network were analyzed, and core drugs were screened. The TCMSP platform was used to screen the active ingredients of core drugs and predict the targets. Immune-related targets were screened from disease databases OMIM, DrugBank, GeneCards and Uniprot to obtain the intersection targets of core Chinese medicine and immunity. Protein interaction (PPI) analysis and gene ontology (GO) function and pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed for intersection targets. Molecular docking technology was used to verify the active ingredients and key targets of the drug. RESULTS According to the analysis of Chinese herbal compound patents, five core drugs were obtained: Astragalus, ginseng, Wolfberry, poria, Angelica. The effective active ingredients corresponding to 214 targets, 1 784 immune-related targets, 55 intersection targets, the key action targets are RELA, TNF, TP53, IL-6. The results of GO analysis showed that the main regulation aspects were the response to bacteria origin molecules, the response to lipopolysaccharide, and the response to biological stimuli. There were 160 KEGG pathways, mainly involving lipid and atherosclerosis, Chagas disease, TNF signaling pathway, cancer pathway and other signaling pathways. The molecular docking results showed that quercetin and kaempferol could bind to RELA, TNF, TP53 and IL-6. CONCLUSION The traditional Chinese medicines used in the patent literature to enhance immunity are mainly deficiency tonic drugs, and the main effective components are quercetin, kaempferol and other compounds. RELA, TNF, TP53 and IL-6 are the key targets, which play the role of enhancing immunity through multiple pathways such as lipid, atherosclerosis, Chagas disease, TNF signaling pathway and cancer pathway.

Clinical Practice Guidelines for TCM in Children with Adenoidal Hypertrophy

YUAN Bin, JIANG Zhiyan, MA Huaan, HAN Mei, LIU Zhuyun, REN Xianzhi, LI Weiwei, WANG Sumei, ZHANG Xueqing, ZHU Xiaohui, WANG Lei, HU Chanchan, MA Jun, WANG Tianhan, LI Shuo

2024, 40(2): 184-189. doi: 10.14148/j.issn.1672-0482.2024.0184

[Abstract](451) [FullText HTML] (25) [PDF 1091KB](73)

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Literature related to children's adenoid hypertrophy was retrieved to form an expert questionnaire. According to the group standard writing rules of the China Association of Chinese Medicine, the peer consultation, quality evaluation and suitability evaluation were completed through three rounds of Delphi expert questionnaire surveys and expert discussion meetings, and the Clinical Practice Guidelines for TCM in Children with Adenoidal Hypertrophy was finally formed. The guidelines have been formulated to clarify the scope of application of the guidelines, normative reference documents, terms and definitions, diagnosis, syndrome differentiation, treatment, prevention and care, and to provide an important reference for the clinical practice and diagnosis and treatment norms of traditional Chinese medicine for children with adenoid hypertrophy.

Clinical Efficacy of Wandai Decoction in Preventing and Treating VVC with Spleen Deficiency-Dampness Abundance Syndrome and Its Impact on DNA Damage

HAN Yue, SHEN Su, FU Jie, REN Qingling

2024, 40(2): 190-195. doi: 10.14148/j.issn.1672-0482.2024.0190

[Abstract](79) [FullText HTML] (15) [PDF 1127KB](15)

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OBJECTIVE To explore the clinical efficacy of Wandai Decoction in the treatment of vulvovaginal candidiasis (VVC) with spleen deficiency-dampness abundance and its effect on DNA damage. METHODS 70 VVC patients with spleen deficiency-dampness abundance who met the inclusion criteria were randomly divided into Wandai Decoction and fluconazole groups, 35 cases each. The fluconazole group took 150 mg fluconazole orally once; the Wandai Decoction group took Wandai Decoction orally for 14 d. After treatment, changes in TCM syndrome scores of the two groups of patients were compared; the clinical cure (test of cure, TOC) rate and clinical improvement (CI) rate of the two groups of patients were evaluated; 8-hydroxydeoxyguanosine (8-OHDG) in the vaginal lavage fluid was detected by colorimetry to assess DNA damage; clinical complete remission (follow up, FU), mycological negative conversion and recurrence rate were evaluated 3 months after treatment. RESULTS After treatment, the TCM syndrome scores of the two groups of patients were improved to varying degrees (P < 0.05, P < 0.01); the Wandai Decoction group was superior to the fluconazole group (P < 0.05, P < 0.01); there was no significant difference in TOC, CI, mycology negative conversion between the Wandai Decoction group and the fluconazole group (P>0.05); however, the FU and recurrence rate of the Wandai Decoction group were significantly better than those of the fluconazole group (P < 0.05, P < 0.01). After treatment, the expression of 8-OHDG in the vaginal lavage fluid of the fluconazole group increased significantly (P < 0.001), while there was no significant change in the Wandai Decoction group, which was significantly lower than that of the fluconazole group (P < 0.001). CONCLUSION The overall clinical efficacy of Wandai Decoction is equivalent to that of fluconazole, but it is better than fluconazole in improving TCM syndromes and preventing recurrence, with the advantage of not aggravating DNA damage in vaginal cells.

Exploration on Differentiation and Treatment of Gastroesophageal Reflux Disease under the Theory of Earth Being Dredged by Wood

LI Jinru, LONG Dan, ZHU Ying

2024, 40(2): 196-200. doi: 10.14148/j.issn.1672-0482.2024.0196

[Abstract](89) [FullText HTML] (11) [PDF 1068KB](7)

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This article summarizes the understanding of Professor Zhu Ying, a guidance teacher for the inheritance of academic experience of national senior traditional Chinese medicine experts, on the theoretical connotation of "earth being dredged by wood" in The Yellow Emperor's Inner Classic, and her experience in treating gastroesophageal reflux disease from the perspective of "earth being dredged by wood". It is believed that the pathogenesis of this disease is the imbalance of the spleen and stomach, failure of gallbladder qi to fall, rise of heart fire, and out-of-control kidney water; the treatment should be based on the principles and methods of soothing the liver and dispersing the gallbladder, coordinating the rise and fall of the spleen and stomach qi, stalling the heart fire and relieving depression, restraining and reducing the burning and dry stomach fire, supporting the spleen and aiding the kidney, and cultivating and nourishing the weak wood. The key is to restore the normal state of the growth and restraint of the wood and soil.

Regulatory Mechanism and New Research Ideas on Tongue Coating Formation

ZHANG Junfeng, ZHAN Zhen

2024, 40(2): 201-208. doi: 10.14148/j.issn.1672-0482.2024.0201

[Abstract](88) [FullText HTML] (39) [PDF 2384KB](15)

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Tongue coating (TC) is a layer of coating on the surface of the tongue dorsal mucosa, and observing its variable color and property is an important content of tongue diagnosis in traditional Chinese medicine (TCM). A large amount of existing evidence has showed that TC images and its material composition have important diagnostic value for systemic diseases, which constructs the modern scientific foundation for the scientific connotation of observing TC to assist clinical syndrome differentiation, prescription, and medication. However, it is still wanting in-depth exploration on the cellular and molecular biological mechanisms underlying TC formation. The current review systematically summarizes the main research literatures, proposes the potential regulatory mechanism of the immuno-neuro-endocrine system, biological toxins, and physical factors in the development of tongue coating, and draws upon innovative approach to exploring the biological mechanism of TC microenvironment including microorganisms, immunity, endocrine, mechanical stretching, temperature, etc. These advances provide valuable research ideas and technical platform for in-depth exploration on the biological mechanisms of TC variety, and promote the scientific research and clinical application of tongue diagnosis.

Research Progress on the Regulation of Differentiation and Function of Stem Cells by Traditional Chinese Medicine and Its Active Substances

ZHANG Teng, SONG Mengyao, QIAN Cheng, ZOU Wei, WANG Aiyun, LU Yin

2024, 40(2): 209-216. doi: 10.14148/j.issn.1672-0482.2024.0209

[Abstract](182) [FullText HTML] (22) [PDF 1944KB](25)

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Traditional Chinese medicine is a treasure of Chinese civilization, which has undergone thousands of years of precipitation and clinical verification. According to the theory of traditional Chinese medicine, ancient doctors used natural medicines and natural means to promote self-regulation and damage repair of the body. In recent years, as an important tool to repair body damage, stem cells have set off a research upsurge. With the continuous deepening of stem cell research and the continuous expansion of the fields involved, the potential correlation between traditional Chinese medicine and stem cells has attracted more and more domestic and foreign researchers to invest in research. This paper expounds regenerative medicine and stem cells, the advantages of Chinese medicine in regulating stem cells, and the opportunities and challenges faced by Chinese medicine and regenerative medicine, and reviews the current research trends and frontier trends in this field, in order to provide useful references for subsequent studies.

2024 Vol. 40, No. 2

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