南京中医药大学学报

Analysis of the Application Ideas and Medicines Compatibility Rules of the Formulas for Supporting the Healthy Energy Based on the Pathogenesis Theory of Cancer Toxin

TAN Feng, LI Liu, TANG Dong-xin, SHEN Wei-xing, WU Mian-hua, CHENG Hai-bo, SUN Dong-dong

2022, 38(2): 93-97. doi: 10.14148/j.issn.1672-0482.2022.0093

[Abstract](626) [FullText HTML] (196) [PDF 1006KB](66)

Abstract:
Professor Zhou Zhongying's experience in the differentiation treatment of tumors was reviewed from the pathogenesis theory of cancer toxin, and the dialectical relationship between "supporting the healthy energy, tonifying the deficiency" and "fighting against cancer and detoxifying" was elaborated. We proposed a tumor treating strategy with fighting against cancer and detoxifying as the core treatment, supplemented by strengthening the spleen and stomach to support the healthy energy. According to the pathogenic characteristics that the healthy energy is prone to cancer toxin, it is suggested that special attention should be paid to the application of the treatment method and related formulas to benefit qi and nourish yin in supporting the healthy energy combined with tonifying the deficiency, which can provide guidance and reference for clinical anti-tumor treatment.

Analysis of the Formulation Idea and Main Treatment Characteristics of Jinshui Liujun Decoction

QIN Huan, SHI Qing-wu, ZENG Ni, WU Cheng-yan

2022, 38(2): 98-102. doi: 10.14148/j.issn.1672-0482.2022.0098

[Abstract](340) [FullText HTML] (251) [PDF 999KB](67)

Abstract:
Through compiling and studying the related literature of Jinshui Liujun Decoction, we find that this formula is created based on Erchen Decoction and Zhenyuan Yin. Especially in drying dampness and dissolving phlegm aspects, rehmanniae radix praeparata and angelicae sinensis radix are added into it to warm and supplement genuine yin for treating deficiency-cold, highlighting the formulation idea of tonifying original qi, as well as warming the spleen and strengthening the kidney. In addition, this formula was used by Zhang Jingyue for treating 19 kinds of diseases and syndromes, and it was also widely used by later generations of physicians with flexible modifications. Therefore, the study on the medicine compatibility characteristics and the formulation idea of Jinshui Liujun Decoction is of great practical value and guidance for the later generations to understand Zhang Jingyue's harmonization treatment method, to study his academic idea of warming and supplementing genuine yin, and to broaden the clinical ideas of treating phlegm along with panting syndrome.

Clinical Study on the Treatment of Severe Pneumonia Combined with Acute Gastrointestinal Injury by Supplemented Xuanbai Chengqi Decoction

HE Jin-bo, MAO Zheng-rong, SONG Peng-yang

2022, 38(2): 103-108. doi: 10.14148/j.issn.1672-0482.2022.0103

[Abstract](243) [FullText HTML] (95) [PDF 1027KB](24)

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OBJECTIVE To evaluate the clinical efficacy of Supplemented Xuanbai Chengqi Decoction in the treatment of severe pneumonia combined with acute gastrointestinal injury (AGI). METHODS In a randomized controlled study, 76 patients with severe pneumonia combined with AGI were randomly divided into the control group and the treatment group, 38 patients in each group. The control group was given conventional western medical treatment, including active treatment of protopathy and complicating disease. In addition, early administration of enteral nutrition and gastrointestinal excitomotor were given in the group. The patients in the treatment group were treated with supplemented Xuanbai Chengqi Decoction intranasally on top of the treatments in the control group. The course of treatment was 7 days and the clinical efficacy of the two groups was evaluated before treatment and 7 days after treatment. The changes of gastrointestinal function indexes [Gastrointestinal failure (GIF) score, enteral nutrition daily feeding amount and intraabdominal pressure (IAP)] were measured before treatment, 3 and 7 days after the treatment separately. Motilin (MTL) levels and diamine oxidase (DAO) activity were measured by ELISA. Besides, the duration of mechanical ventilation, ICU stay length and 28-d mortality rate were recorded. RESULTS After treatment, the total effective rate of the treatment group was better than that of the control group (P < 0.05). The GIF, enteral nutrition daily feeding amount and IAP of the treatment group significantly improved (P < 0.01). However, the GIF, enteral nutrition daily feeding amount and IAP of the treatment group showed better Results than those of the control group after 7 days of treatment (P < 0.05). In addition, the serum MTL level and DAO activity were significantly increased in both groups after 3 and 7 days of treatment (P < 0.01). However, the MTL levels in the treatment group were significantly higher than those in the control group after 3 and 7 days of treatment (P < 0.05, P < 0.01). The duration of mechanical ventilation and ICU stay length in the treatment group were significantly less than those in the control group (P < 0.01). Besides, there was no significant difference in the 28-d mortality rate between the two groups (P > 0.05). CONCLUSION Supplemented Xuanbai Chengqi Decoction can effectively improve the gastrointestinal function of patients with severe pneumonia combined with AGI, shorten the ICU stay length, and promote the recovery of patients.

Clinical Study on the Treatment of Chronic Sinusitis with Deficiency-Cold of Lung Qi by Wenyang Zhiti Decoction

XU Xin-ming, CAI Ji-tang, LI Jing-bo, WANG Jun-jie, WU Zi-lu

2022, 38(2): 109-114. doi: 10.14148/j.issn.1672-0482.2022.0109

[Abstract](207) [FullText HTML] (129) [PDF 1042KB](28)

Abstract:
OBJECTIVE To investigate the clinical efficacy and possible mechanism of Wenyang Zhiti Decoction in the treatment of chronic sinusitis with deficiency-cold of lung qi. METHODS A total of 160 patients with chronic sinusitis with deficiency-cold of lung qi were randomly divided into the control group and the treatment group of 80 patients each. In the control group, enteric soft capsule and mometasone furoate nasal spray were administered. While in the treatment group, Wenyang Zhiti Decoction was given as local administration of drugs. The course of treatment was 28 days. In addition, the clinical efficacy, VAS score, traditional Chinese medicine (TCM) syndrome score, Lund-Mackay score, as well as nasal mucosal cilia clearance speed and rate of the two groups were evaluated before and after treatment. The changes of serum inflammatory factors (IL-17, IL-33, IL-37) and serum immunomodulatory factors (sTim1, sTim3, sTim4) were detected by ELISA to observe the incidence of adverse reactions. RESULTS After treatment, the efficiency of the treatment group was significantly increased than that of the control group (P < 0.01). The TCM syndrome score, VAS score and Lund-Mackay score of sinus CT were significantly reduced in both groups (P < 0.05, P < 0.01), but the Results in the treatment group were better than those in the control group (P < 0.01). In addition, the clearance speed and rate of nasal mucous membrane cilia were significantly increased in both groups (P < 0.05, P < 0.01), but the Results in the treatment group were better than those in the control group (P < 0.01). Besides, the serum IL-17, IL-33, sTim1, sTim3 and sTim4 were significantly reduced in both groups (P < 0.05, P < 0.01), but the Results in the treatment group were better than those in the control group (P < 0.01). The serum IL-37 level was significantly increased in both groups (P < 0.05, P < 0.01), but the result in the treatment group was better than that in the control group (P < 0.01). In addition, no significant adverse effects were observed in both groups. CONCLUSION Wenyang Zhiti Decoction can effectively improve the clinical symptoms of patients with chronic sinusitis with deficiency-cold of lung qi, and its mechanism may be related to the regulation of serum inflammatory factors and immunomodulatory factors.

Study on the Inhibitory Effect of Huangqi-Ezhu-Chonglou Combination on Metastasis of Colon Cancer by Reducing Vascular Endothelial Permeability

LIU Tian-tian, BIAN Yong, GUAN Han-qing, LIANG Yan, GUO Wen-hui, YU Qian-hui, TANG De-cai

2022, 38(2): 115-121. doi: 10.14148/j.issn.1672-0482.2022.0115

[Abstract](367) [FullText HTML] (244) [PDF 2376KB](39)

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OBJECTIVE To observe the effects of Huangqi-Ezhu-Chonglou combination (HEC) on the permeability and tight junction of human umbilical vein endothelial cells (HUVEC) and to explore the mechanism of HEC on inhibiting the hematogenous metastasis of colon cancer cells (HCT116). METHODS Control serum and HEC-medicated serum were prepared. The co-culture system of HUVEC-HCT116 was constructed and divided into single culture control group, co-culture model group and HEC low, medium and high dose (2.1, 4.2, 8.4 g ·kg^-1) groups. In addition, HUVEC single culture control group was set up. Cell proliferation was detected by CCK-8 method; FITC-dextran method and transwell method were used to detect the permeability of HUVEC and the number of cross endothelial migration of HCT116 cells; The distribution of ZO-1 protein in HUVEC was detected by immunofluorescence; The protein expressions of RhoA, ROCK and ZO-1 were detected by Western blot. RESULTS The proliferation of HUVEC was inhibited after 48 h of treatment with different doses of HEC medicated serum (P < 0.01). Compared with the single culture control group, FITC-dextran penetration and the number of transendothelial migration of HCT116 in the co-culture model group significantly increased (P < 0.01), the expression of ZO-1 was down-regulated (P < 0.01), and the expressions of RhoA and ROCK were up-regulated(P < 0.01); Compared with the co-culture model group, the FITC-dextran penetration and the number of transendothelial migration of HCT116 in the HEC group significantly decreased (P < 0.05), the expression of ZO-1 protein was up-regulated (P < 0.05), and the expressions of RhoA and ROCK were down-regulated in a dose-dependent manner (P < 0.05). CONCLUSION HEC may regulate the expression of tight junction associated proteins ZO-1 in HUVEC by inhibiting RhoA/ROCK pathway, reduce vascular endothelial permeability, and then inhibit colon cancer hematogenous metastasis.

Molecular Mechanism Research of Wenshen Yanggan Decoction on Dopaminergic Neuron Injury in MPTP-Induced Parkinson's Disease

XU Cheng-cheng, LU Yan, TANG Li-li, LIANG Yan, ZHAO Yang

2022, 38(2): 122-128. doi: 10.14148/j.issn.1672-0482.2022.0122

[Abstract](176) [FullText HTML] (73) [PDF 5404KB](14)

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OBJECTIVE To explore the potential mechanism of Wenshen Yanggan Decoction on protecting dopaminergic neurons and delaying the process of Parkinson's disease (PD). METHODS Fifty mice were randomly divided into 5 groups (n=10): control group, model group, Wenshen Yanggan Decoction high dose group, Wenshen Yanggan Decoction low dose group and amantadine group. Except for the control group, mice were injected intraperitoneally with 30 mg · kg^-1 of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) twice a week for 4 consecutive weeks to establish a Parkinson's disease (PD) mouse model. The mice were administrated with Wenshen Yanggan Decoction by gavage once a day for 3 weeks. The behavioral effects of mice were observed. Immunohistochemistry and Nissl staining were used to detect the number of DA neurons. The contents of DA, 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in pathological tissues were measured by HPLC. Colorimetric method was used to determine the activities of ATPase and mitochondrial respiratory chain ComplexⅠ in the substantia nigra. The protein expression levels of PINK1, Parkin, VDAC1, LC3Ⅱ/Ⅰ, and P62 were detected by Western blot. RESULTS The behavioral tests showed that compared with the model group, Wenshen Yanggan Decoction significantly improved the muscle strength and exercise balance of mice (P < 0.01), and prolonged the duration of mice on the roller (P < 0.01). The Results of the immunohistochemistry demonstrated that a large number of TH immunoreactive cells and obvious cell protrusions were observed in Wenshen Yanggan Decoction high dose group, which was consistent with the Results of the Nissl staining assay. In addition, Wenshen Yanggan Decoction significantly increased the levels of DA and HVA (P < 0.05, P < 0.01) in the striatum. Furthermore, treatment with the high dose Wenshen Yanggan Decoction profoundly promoted the activities of ATPase and ComplexⅠ(P < 0.01), up-regulated the protein levels of PINK1 (P < 0.05), Parkin (P < 0.01) and LC3Ⅱ/Ⅰ (P < 0.01), and inhibited P62 protein expression (P < 0.01) in MPTP-induced mice. CONCLUSION Wenshen Yanggan Decoction can improve mitochondrial function, and provide neuroprotective effect in PD by activating PINK1/Parkin mediated mitochondrial autophagy pathway.

Effects of Pine Massoniana Needle Extract on Androgenetic Alopecia Model Rats via Nrf2-ARE Mediated Signaling Pathway

ZHU Hong-liu, WEI Yue-gang, MIN Zhong-sheng, GAO Yi-hong, YANG Jian-qiu

2022, 38(2): 129-135. doi: 10.14148/j.issn.1672-0482.2022.0129

[Abstract](241) [FullText HTML] (101) [PDF 3907KB](21)

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OBJECTIVE To investigate the hair growth-promoting activity and the possible mechanism of pine massoniana needle extract (PMNE) on mice by using the dihydrotestosterone (DHT) induced androgenetic alopecia (AGA) model. METHODS 48 C57BL/6 mice were randomly divided into 6 groups: control group without any treatment, DHT group (30 mg ·d^-1 DHT only), procyanidin group (DHT+procyanidin B2 5 mg ·kg^-1), PMNE low dose group (DHT+PMNE 4 mg ·kg^-1), PMNE medium dose group (DHT+PMNE 8 mg ·kg^-1) and PMNE high dose group (DHT+PMNE 12 mg ·kg^-1). PMNE and procyanidin B2 were given orally every day. The hair growth of mice was observed and evaluated at d7, d14, d21 and d28 after administration. The circle skins of all mice were harvested at the same depilated aera after 28 days. The hair from these skins was weighed. Some skin tissue section was obtained for HE staining, and the number of hair follicles were calculated. Some skin tissues were obtained to detect the contents of ROS and MDA and the mRNA and protein levels of Keap1, Nrf2, NQO1, HO-1 and TGF-β1 by qPCR and Western blot. RESULTS PMNE promoted the hair growth of AGA mice and showed a dose-dependent effect. PMNE of 12 mg ·kg^-1 could alleviate the DHT-induced AGA in mice and achieve a similar effect to procyanidin B2. PMNE reduced the contents of ROS and MDA, promoted the mRNA and protein expressions of Nrf2, NQO1, HO-1 and reduced the mRNA and protein expressions of Keap1 and TGF-β1. CONCLUSIONS PMNE can promote the hair growth of AGA mice by regulating the Nrf2-ARE signaling pathway.

Study on the Anti-Colorectal Cancer Mechanism of Jianpi Jiedu Decoction Based on Network Pharmacology

QIU Ya-lan, GAO Jing-dong, LIU Min, ZHANG Lei, SONG Qing

2022, 38(2): 136-146. doi: 10.14148/j.issn.1672-0482.2022.0136

[Abstract](321) [FullText HTML] (167) [PDF 6155KB](40)

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OBJECTIVE To explore the anti-tumor mechanism of Jianpi Jiedu Decoction (JPJDD) based on network pharmacology and molecular docking. METHODS TCMSP, TCMID and ETCM databases were used to predict the active ingredients and the targets of JPJDD, and GeneCards database was used to search tumor related targets and match the targets above. The common targets were imported into Cytoscape 3.7.2 to construct the PPI network and select the key targets by network topology analysis. GO enrichment analysis and KEGG pathway enrichment analysis were performed by R 3.6.3 software. Finally the Autodock 2.4 was used for molecular docking to predict the binding degree of the active ingredients to target genes. In addition, the key targets were verified by cell experiments. RESULTS 24 kinds of active ingredients and 86 tumor related targets were identified, including the key targets of PTGS2, HSP90AA1 and PRSS1, which were obtained by PPI network topology analysis. The molecular docking showed that all of the key targets had good binding activity with quercetin and kaempferol. GO enrichment analysis and KEGG pathway enrichment analysis suggested that JPJDD may play the role of anti-tumor through PI3K/Akt signal pathway and MAPK signal pathway. The results showed that JPJDD inhibited both the protein and mRNA expressions of PTGS2 and p38MAPK, but after silencing PTGS2 gene, the expression of downstream gene p38MAPK and the effect on colorectal cancer cells metastasis had no obvious response to JPJDD. CONCLUSION JPJDD can inhibit the metastasis of colorectal cancer via PTGS2 mediated p38MAPK signaling pathway.

Effect of Xiangsha Liujunzi Decoction Regulating CircRNA-0067835 on Cholesterol Efflux in Hyperlipidemia Rats with Spleen Deficiency

MENG Jia-wei, SONG Nan, WANG Ying, CHEN Si, WANG Qun, ZHAO Qiu-yu, JIA Lian-qun

2022, 38(2): 147-153. doi: 10.14148/j.issn.1672-0482.2022.0147

[Abstract](189) [FullText HTML] (65) [PDF 2031KB](14)

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OBJECTIVE To investigate the effect of Xiangsha Liujunzi Decoction on lipid deposition in rats with spleen deficiency and hyperlipidemia and its molecular biological mechanism. METHODS Thirty SD rats were randomly divided into 5 groups: blank control group, high-fat group, spleen-deficiency and high-fat group, high-dose Xiangsha Liujunzi Decoction group, and normal-dose Xiangsha Liujunzi Decoction group. A compound method of unregulated diet and swimming until exhaustion was used for 15 days to establish a model of spleen deficiency. After feeding the high-fat diet for 10 weeks, the blood lipid levels of rats were tested to confirm that the hyperlipidemia model was successfully replicated. Xiangsha Liujunzi Decoction high-dose group and normal-dose group were given Xiangsha Liujunzi Decoction of 11.34, 5.67 g·kg^-1·d^-1 intragastrically, the other 3 groups were given corresponding volume of normal saline intragastrically, and the samples were taken 4 weeks later. The blood lipid levels of the 5 groups of rats were detected. HE staining was used to observe the pathological changes of the liver. qPCR method was used to detect CircRNA-0067835 and miR-155 gene levels. The expression levels of PPARγ, LXR, ABCA1, ABCG1 mRNA and protein were detected by qPCR and Western blot. RESULTS Compared with the blank control group, HE staining of high-fat group and spleen-deficiency and high-fat group showed obvious swollen liver cells and clear fatty vacuoles. The serum contents of TC, TG, LDL-C and the expression of miR-155 increased (P < 0.01), HDL-C level, the expression of CircRNA-0067835, the mRNA and protein expressions of PPARγ, LXR, ABCA1, ABCG1 all decreased (P < 0.05, P < 0.01). Compared with the spleen-deficiency and high-fat group, the TC, TG and LDL-C levels of Xiangsha Liujunzi Decoction high-dose group and normal-dose group significantly reduced (P < 0.01), and the HDL-C level significantly increased (P < 0.01), swollen liver cells were significantly improved, fatty vacuoles reduced, miR-155 expression decreased (P < 0.01), CircRNA-0067835 expression increased (P < 0.01), PPARγ, LXR, ABCA1, ABCG1 mRNA and protein expressions all increased (P < 0.05, P < 0.01). There was statistical significance between Xiangsha Liujunzi Decoction high-dose group and the normal-dose group (P < 0.05, P < 0.01). CONCLUSION Xiangsha Liujunzi Decoction may improve cholesterol metabolism in hyperlipidemia rats by influencing CircRNA-0067835 to regulate the cholesterol efflux process mediated by PPARγ, thereby preventing and treating dyslipidemia.

Preparation and Physicochemical Properties of Hawthorn Leaf Extract Phospholipid Complex

WANG Yuan-yuan, CAO Jie-nan, XIA Meng-ying, YAN Guo-jun, PAN Jin-huo

2022, 38(2): 154-161. doi: 10.14148/j.issn.1672-0482.2022.0154

[Abstract](177) [FullText HTML] (67) [PDF 2302KB](14)

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OBJECTIVE To optimize the preparation method of hawthorn leaf extract phospholipid complex and research its physicochemical properties. METHODS The mass fraction weight coefficient method was used to characterize the overall properties of the flavonoids of the hawthorn leaf extract. On the basis of single factor investigation, the orthogonal design experiment was used to optimize the preparation method, taking the component compound rate and mass compound rate as the index. It was characterized by scanning electron microscopy, UV spectrum, infrared spectrum and X-ray diffraction. While its apparent oil-water partition coefficient was also investigated. RESULTS The best preparation method of hawthorn leaf extract phospholipid complex is to use absolute ethanol as the compound solvent, the mass concentration of hawthorn leaf extract is 1 mg ·mL^-1, the drug-to-lipid ratio is 1 ∶ 4, and the reaction is compounded at 60 ℃ for 1.5 h. Scanning electron microscopy, X-ray diffraction analysis, etc. verified the formation of hawthorn leaf extract phospholipid complex, and the apparent oil-water partition coefficient of different pH was improved compared with the crude drug. CONCLUSION The preparation method of hawthorn leaf extract phospholipid complex is reasonable and feasible, and the fat solubility is improved.

Differentiation Treatment of Palpitations from Contention and Binding between Heat and Stasis

ZOU Chong, FANG Zhu-yuan

2022, 38(2): 162-165. doi: 10.14148/j.issn.1672-0482.2022.0162

[Abstract](273) [FullText HTML] (163) [PDF 978KB](32)

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The academic experience of Professor Fang Zhuyuan (the first Qi Huang scholar) is summarized in terms of the arrhythmia pathogenesis of contention and binding between heat and stasis syndrome combined with the prescriptions and medicines used in the clinical treatment. Professor Fang believes that contention and binding between heat and stasis is one of the keys to the pathogenesis of palpitations. Thus, the treatment should be based on dissolving stasis and clearing heat, while taking into account treating both qi and yin, as well as zang-fu organs. In addition, the treatment of palpitations guided by this academic thought has achieved satisfying therapeutic effects.

Discussions on the Quality Controls of Chinese Herbal Decoction Pieces Based on Requirements for Drug Technical Evaluation

WANG Ling-ling, ZHANG Yong-wen

2022, 38(2): 166-171. doi: 10.14148/j.issn.1672-0482.2022.0166

[Abstract](301) [FullText HTML] (92) [PDF 1043KB](25)

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The prescription of Chinese herbal decoction pieces is the material basis for the treatment and prevention of diseases. It is used not only in the clinical formulation of traditional Chinese medicine(TCM), but also in the production of TCM. Its quality directly relates to the safety and effectiveness of the medicine used by the people. Combining the quality problems of Chinese herbal decoction pieces in the past five years, this article analyzes the problems related to the standard of Chinese herbal decoction pieces in the process of drug evaluation, and sorts out the basic requirements of Chinese herbal decoction pieces for new TCM, new TCM drug on classic famous prescription, modified new TCM, TCM with same name and prescription, and marketed TCM. It is convenient to strengthen the quality control of Chinese herbal decoction pieces, and provide a useful reference for the quality control of Chinese herbal decoction pieces and related research.

Progress Research of Okra Plant Resources

PU Wei-ya, ZHANG Yu

2022, 38(2): 172-179. doi: 10.14148/j.issn.1672-0482.2022.0172

[Abstract](329) [FullText HTML] (114) [PDF 1651KB](18)

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Most of okra are medicinal plant resources in our country, which are widely used for medicinal, edible, feed, industrial, and ornamental purposes. This article reviews the classification, habitat and distribution, types of medicinal resources, main medicinal ingredients, pharmacological effects and application prospects of okra, and has a comprehensive understanding of the resources of okra plants.

Analysis of the Research Status and Hot Spot of Intelligent Four-Diagnosis in TCM

LI Hong-yan, LI Can, LANG Xu-feng, YANG Tao, ZHOU Zuo-jian, ZHAN Li-bin

2022, 38(2): 180-186. doi: 10.14148/j.issn.1672-0482.2022.0180

[Abstract](497) [FullText HTML] (1601) [PDF 7770KB](141)

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As the basic means of traditional Chinese medicine (TCM) diagnosis and syndrome differentiation, the four-diagnosis of TCM has been further inherited and innovated under the empowerment of artificial intelligence in recent years. In this paper, we summarized the Chinese and English literature of the four-diagnosis intelligent research of TCM in the past ten years, analyzed its current research status, completed the visual analysis based on the knowledge graph, and further discussed the research hot-spots in this field. Finally, based on the existing research status, we proposed thinking and prospects for the next development of the intellectualization of the TCM four-diagnostics, in order to provide reference value and significance for the future research in this area.

2022 Vol. 38, No. 2

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