2019 Vol. 35, No. 6

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Examples of Syndrome Differentiation for Common symptoms of Tumor in Traditional Chinese Medicine
ZHOU Zhong-ying
2019, 35(6): 631-633.
[Abstract](731) [PDF 1083KB](678)
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Common symptoms of tumor were analyzed through syndrome differentiation in traditional Chinese medicine (TCM) and some of them were given to illustrate， including cancer pain， fever， bleeding， anemia， vomiting， diarrhea， constipation and swelling. It is believed that cancer patients are deficient as a whole while excess at the cancerous local site. For such deficiency-excess complicated syndrome， it is doomed to be incurable once delayed to terminal stage. By then， the patient can neither be treated with reducing nor reinforcing method， but only with palliative care assisted by strengthening stomach to help govern intake.
Application of Phase-Pivot Dialectic in Clinical Diagnosis and Treatment of Orthopedics in Traditional Chinese Medicine
WANG Pei-min, XING Run-lin
2019, 35(6): 634-637.
[Abstract](624) [PDF 1108KB](460)
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The paper put forward the academic concept of phase-pivot syndrome. Phase refers to time phase and place phase. Pivot refers to helm. The syndrome differentiation of time phase and place phase emphasizes the combination of local and overall syndrome differentiation in orthopedic diseases. It mainly includes the combination of immediate syndrome differentiation in acute attack period and basic syndrome differentiation in chronic prolongation period (time phase) and the combination of specific syndrome differentiation of the affected part and physical basis syndrome (place phase). The helm is mainly aimed at the chronic diseases which are are difficult for orthopedic experts to heal. Grasping the main contradiction and controlling the core symptoms are key to grasping the essential of the phase-pivot dialectic. It is believed that the introduction of phase-pivot dialectic system to the clinical syndrome differentiation of orthopedics in traditional Chinese medicine can not only improve the pertinence of principle-method-recipe-medicines in orthopedics， but also help shorten the learning curve of young doctors.
Discussion on the Thermodynamical Mechanism of Reinforcing and Reducing in Moxibustion
XIEQi-qi, WANHong-mian
2019, 35(6): 638-639.
[Abstract](530) [PDF 1073KB](550)
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The paper elaborated the specific methods of reinforcing and reducing in moxibustion recorded in the literature and probed its mechanism from the perspective of thermodynamics. It holds that reinforcing in moxibustion is to enable the heat transfer rate of smoldering moxa faster than the airflow rate of the combustion zone so as to allow the heat of penetrate into human body. On the contrary， reducing in moxibustion is to enable the airflow rate of the combustion zone faster than the heat transfer rate of rapid burning moxa. As a result， the temperature of the skin surface is lower than that of the upper moxa. Therefore， pathogen could be drained with the intensity of upwards airflow.
Effect of Acupuncture at Weishu Combined with Zhongwan on the Regional Homogenity of Resting-State Brain Function and Electrogastrogram for Patients with Functional Dyspepsia
GUANYuan-yuan, CAIRong-lin, XIAOHong-bo, CHUHao-ran, LIChuan-fu, SHENGuo-ming
2019, 35(6): 640-645.
[Abstract](630) [PDF 1540KB](573)
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OBJECTIVE To observe the effect of acupuncture at Weishu (BL21) combined with Zhongwan (RN12) and acupuncture at non-combined acupoints on regional homogeneity (ReHo) of resting-state brain function and the electrogastrogram (EGG) in patients with functional dyspepsia (FD)， and to explore the central mechanism of regulating the gastric motility of FD patients by acupuncture at BL21 and RN12. METHODS 37 FD patients were randomly divided into combined acupoints group (19 cases) and non-combined acupoints group (18 cases). The two groups were treated with bilateral BL21+RN21 acupuncture therapy and non-acupoints acupuncture therapy of the same segment separately. At the same time， 19 healthy people were enrolled as the control group. The resting-state functional magnetic resonance imaging scan (rs-fMRI) and EGG test were performed before and after treatment. The changes of ReHo and EGG were observed before and after treatment. Additionally， the correlation analysis between the changes of ReHo and gastric motility was conducted. RESULTS ① After treatment， the amplitude and frequency of EGG in the combined acupoints group increased (P<0.05，P<0.01). In the non-combined acupoints group， the amplitude of EGG increased (P<0.01)， and the frequency was not significantly different from that before treatment (P>0.05). ② Compared with the control group， the ReHo values of FD patients in different regions of the brain such as cerebellum， frontal lobe， cingulate gyrus， insula and temporal lobe were abnormal. In the combined acupoints group， the ReHo values of fusiform gyrus， posterior cingulate gyrus and other brain areas increased in FD patients after treatment (P<0.05)， and that of hippocampus and middle frontal gyrus decreased (P<0.05). In the non-combined acupoints group， the ReHo values of cerebellum， superior frontal gyrus， middle frontal gyrus， anterior cuneiform lobe， angular gyrus and other brain regions of FD patients changed after acupuncture. ③ Correlation analysis showed that the changes of EGG were correlated with the changes of superior temporal gyrus， middle temporal gyrus， posterior cingulate gyrus， middle frontal gyrus and hippocampus in the combined acupoints group. There was no significant correlation between the changes of ReHo values in different brain regions and the change of EGG amplitude in the non-combined acupoints group. CONCLUSION Acupuncture at BL21 combined with RN21 can cause the changes of ReHo values in multiple brain regions of FD patients and its effect on gastric motility is better than that of non-combined acupoints group. Hippocampus， posterior cingulate gyrus， cerebellum and other brain regions may be involved in the regulation of gastric motility. The effect of regulating gastric motility in FD patients by acupuncture at BL21 combined with RN21 may be achieved by the benign regulation of brain regions which is related to limbic system and gastrointestinal sensory matrix.
Clinical Study on Guyang Huashui Formula in Treating Diabetic Macular Edema with Spleen and Kidney Yang Deficiency Type
ZHANGTao-tao, WANGLu-lu, XIAOYan-ping, LIAOLiang
2019, 35(6): 646-650.
[Abstract](619) [PDF 1139KB](488)
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OBJECTIVE To evaluate the clinical efficacy of Guyang Huashui formula in treating diabetic macular edema (DME) with spleen and kidney yang deficiency type. METHODS A prospective randomized controlled trial was conducted to observe 66 patients (66 eyes) with DME outpatients in the department of ophthalmology in Dongfang Hospital from January in 2016 to December in 2018. Patients admitted to the study should meet the diagnostic criteria for moderate and worse international clinical classification of DME and traditional Chinese medicine (TCM) syndrome type of spleen and kidney yang deficiency. Randomly divided into the experimental group and the control group， 33 cases each， who were given Guyang Huashui formula granules or calcium hydroxybenzenesulfonate capsules and Maizhiling tablets for oral treatment for 3 months. The efficacy was evaluated according to the patient's best corrected visual acuity， macular retinal thickness and volume， and TCM syndrome scores. RESULTS 30 patients in each group completed all treatment and observation. After treatment， the best corrected visual acuity in both groups was significantly improved compared with that before treatment (P<0.05， P<0.01 ) and difference between groups has no significant meaning (P>0.05). The thickness of the retinal film in the macular center of the test group was significantly reduced (P<0.01 ). The thickness of the retinal membrane and the average thickness of the retinal membrane in the macular area of the two groups were significantly reduced (P<0.01)， and the test group was significantly better than the control group (P<0.01). In the trial group， the medical syndrome was clearly improved (P<0.05， P<0.01). The two groups were compared and the difference was significant (P<0.05， P<0.01). CONCLUSION Guyang Huashui formula can significantly improve DME patient's vision and reduced retinal thickness in the macular area so as to relieve their TCM syndrome.
A Clinical Study on the Effect of Qingshen Capsule on Chronic Kidney Failure Patients' Kidney Function， Serum α-Smooth Muscle Actin and E-Cadherin
ZHANGLei, JINHua, WANGYi-ping, WANGDong, WENYan, LIUKe
2019, 35(6): 651-654.
[Abstract](692) [PDF 1112KB](424)
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OBJECTIVE To observe the effect of Chinese herbal compound Qingshen capsule on chronic kidney failure patients' kidney function， serum α-smooth muscle actin (α-SMA)and E-cadherin. METHODS 70 chronic kidney failure patients with dampness-heat syndrome were randomly divided into the treatment group and the control group with35 in each. Based on the therapy for the control group， the treatment group was also given Qingshen capsule. The course lasted 12 weeks. After that， 68 patients completed the follow-up， each group 34 cases. The observing parameters included curative effect of traditional Chinese medicine (TCM) syndrome， clinical effect， the level of serum creatinine (Scr)，e-GFR serum α-SMA and E-cadherin before and after treatment. RESULTS After treatment， TCM syndrome effect and clinical effect of the treatment group was better than that of the control group (P<0.05). As for the level of Scr and eGFR， patients in the treatment group has much improved (P<0.05) than those in the control group (P<0.05). The level of serum α-SMA and E-cadherin declined (P<0.05)， lower than that of control group (P<0.05). Patients in the treatment group hadn't been exposed to the side effects of Qingshen capsule. CONCLUSION Qingshen capsule can relive clinical symptoms for chronic kidney failure patients with dampness-heat syndrome， more precisely， degrading Scr level， serum α-SMA and E-cadherin and having good safety.
Clinical Study on Treating Diabetic Retinopathy by Rosiglitazone Combined with Anxue Granules
LIU Hui-rong, ZHANG Feng-mei, ZHAO Ai-xia, LI Hao-yang, LIU Li, ZHANG Meng, SUN Hui-yue
2019, 35(6): 659-663.
[Abstract](511) [PDF 1140KB](481)
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OBJECTIVE To observe the efficacy and safety of rosiglitazone combined with anxue granules in the treatment of diabetic retinopathy (DR). METHODS 210 patients were randomly divided into three groups: Chinese medicine group， western medicine group and combination group. On the basis of regulating blood pressure， blood lipid， blood glucose and other routine treatment， rosiglitazone， anxue granules， rosiglitazone + anxue granules were taken orally by patients in three groups respectively for 60 days. The ophthalmic routine examination indexes (gray value of visual field， thickness of retina， thickness of macular fovea， volume of hemangioma， average defect of visual field and average photosensitivity)， hemodynamic indexes of central retinal artery [peak systolic velocity (PSV)， end-diastolic velocity (EDV)， pulse index (PI)， resistance index (RI)]， serum cytokines [intercellular Adhesion factor-1 (ICAM-1)， endothelin-1 (ET-1)， transforming growth factor-β (TGF-β) and stromal cell derived factor-1 (SDF-1)]， oxidative stress related indicators [triacetate cellulose (TAC)， malondialdehyde (MDA)， lipid hydrogen peroxide (LHP) and total antioxidant capacity (TAOC)] were observed before and after treatment. Total effective rate and incidence of adverse reactions were compared among the three groups. RESULTS 9 cases dropped off during the study. The total effective rate of combined group was 97.1%， higher than that of Chinese medicine group (76.1%) and western medicine group (76.9%) (P<0.05). There was no significant difference in the total effective rate between the Chinese medicine group and western medicine group (P>0.05). The ophthalmic routine examination， retinal central artery blood flow mechanics， serum cytokines and oxidative stress were significantly improved in the combined group than that of Chinese medicine group and western medicine group (P<0.05). The incidence of adverse reactions: Chinese medicine group was 1.5%)， lower than combined group (5.8%) and western medicine group (13.8%) (P<0.05). CONCLUSION Rosiglitazone combined with anxue granules is safe and effective in the treatment of DR， and it is worthy of clinical application.
Selaginella Pulvinata<\i> Extract Exerts Antitumor Efficacy in H22 Tumor-Bearing Mice via Induction of Apoptosis
FANLian, WUYong-ping, QUHui, WUXin-hua, CAOYuan
2019, 35(6): 664-670.
[Abstract](672) [PDF 2028KB](444)
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OBJECTIVE To investigate the in vivo antitumor effect of S. pulvinata extract (SP) and its underlying mechanisms. METHODS A solid tumor H22-transplanted model in mice was established and randomly divided into normal saline control group， 5-FU chemotherapy group (30 mg/kg)， low-dose SP group (63 mg/kg)， and high-dose SP group (189 mg/kg). All mice received respective treatment once daily for 15 days. The tumor weights were monitored， and the inhibition rates as well as organ indexes were calculated. The potential mechanism was investigated using TUNEL staining， qPCR and Western blot analysis. RESULTS Compared with the control group， SP significantly inhibited tumor growth without producing obvious side-effects on body weight and immune organs. Observations from a TUNEL staining experiment demonstrated that SP noticeably induced apoptosis in tumor tissues. Moreover， qPCR showed that SP upregulated the mRNA levels of Bax and downregulated those of Bcl-2， resulting in the release of cytochrome c， which is in agreement with their protein expressions. Further Western blot analysis revealed that SP significantly activated and induced the cleavage of caspase-3， caspase-8 and caspase-9. CONCLUSION The findings clearly demonstrates that the mechanism of SP involves the induction of apoptosis. The study suggests that SP possesses potent in vivo antitumor effects against HCC.
Protection of Danggui Shaoyao San on the Down-Regulated Glucose Transporter in Mice Placental Microvillous Membrane by Controlled Ovarian Hyperstimulation
YAOQi, ZHOUGe, DAIJian-guo, ZHANGLu-yao, HURong-kui
2019, 35(6): 671-675.
[Abstract](779) [PDF 1351KB](473)
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OBJECTIVE To assess the protection of Danggui Shaoyao San on the down-regulated glucose transporter in mouse placental microvillous membrane (MVM) by controlled ovarian hyperstimulation. METHODS IVF blastocysts were transferred to non-stimulated or superovulated pseudopregnant recipient mice. The non-stimulated pseudopregnant recipient mice were non-stimulated recipient mice group. The superovulated pseudopregnant recipient mice were superovulated recipient mice group. The superovulated pseudopregnant recipient mice which received Danggui Shaoyao San treatment were Danggui Shaoyao San treatment group. At E18.5 d， the fetal weight and placental weight were examined. The mRNA expressions of Igfbp1， Igf1and Glut1 in placenta were determined by qPCR. The IGFBP1 and IGF1 protein levels in placental homogenates were determined by ELISA. The GLUT1 protein levels in placental homogenates and MVM were detected by Western blotting. RESULTS At E18.5 d， the mean weights of fetal and placental (P<0.01)， the mRNA for Igf1、Glut1 in placenta (P<0.01)， and GLUT1 protein levels of placental homogenates and MVM (P<0.01) in superovulated recipient mice group were significantly lower compared with non-stimulated recipient mice group. The mRNA for Igfbp1 in placenta of superovulated recipient mice group was significantly higher compared with non-stimulated recipient mice group (P<0.01). Meanwhile， the mean weights of fetal and placenta (P<0.01) ， the mRNA for Igf1， Glut1 in placenta (P<0.05)， and GLUT1 protein levels of placental homogenates and MVM (P<0.05) in Danggui Shaoyao San treatment group were significantly higher compared with those in superovulated recipient mice group. The mRNA for Igfbp1 in placenta of Danggui Shaoyao San treatment group was significantly lower compared with that in superovulated recipient mice group (P<0.05). CONCLUSION Danggui Shaoyao San plays protective effect on the down-regulated glucose transporter in mouse placental MVM by controlled ovarian hyperstimulation. The protection of Danggui Shaoyao San is associated with down-regulated IGFBP1 protein expression and up-regulated IGF1 protein expression in placenta.
Effect of Jasminoidin on Differentiation and Function of Treg Cells and Its Mechanism
BIANYong, ZHANGWen, SHILe, YUBin, YUYun, CUIWei-wei
2019, 35(6): 676-681.
[Abstract](563) [PDF 1876KB](441)
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OBJECTIVE To investigate the effect and mechanism of Jasminoidin on the directional differentiation of Nave T into Treg. METHODS CD4+CD62L+T was isolated from the spleen of mice by magnetic beads and induced to differentiate into Treg. Jasminoidin was used alone， and combined with ERK pathway blocker and JNK blocker. FCM was used to detect the proportion of Treg cells， and Western blot was used to detect the transcription factors of Foxp3， ERK， p-ERK，JNK and p-JNK. The expression of IL-10 were detected by ELISA method. RESULTS The percentage of Treg cells， the expression of Foxp3， p-ERK， p-JNK and the content of IL-10 in Jasminoidin group were significantly higher than those in control group when Jasminoidin was used on the basis of induction alone. On the basis of induction alone， ERK pathway blockers were added before Jasminoidin. The percentage of Treg cells， the expression of Foxp3 and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. On the basis of induction alone， JNK blockers were added before Jasminoidin. The proportion of Treg cells， the expression of Foxp3， and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. CONCLUSION Jasminoidin can promote the directional differentiation of Nave T into Treg and enhance its function， which may be associated with ERK and JNK.
Stress-Induced Dysfunction of Excitatory Amino Acid Transport in Hippocampal Astrocytes of Mice
WANGZhong-li, YIBen-yi, GANLi-jun, LIXiu-li, BIANYao-yao
2019, 35(6): 682-686.
[Abstract](514) [PDF 1666KB](393)
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OBJECTIVE To observe the effect of stress on the excitatory amino acid transport function of hippocampus astrocytes in mice. METHODS 100 male ICR mice were randomly divided into 10 groups， 5 control groups and 5 model groups. The stress model was made by intraperitoneal injection of corticosterone. Ten groups of animals were allocated to five time points (1， 2， 3， 4， 5 weeks). Another 30 male ICR mice were divided into control group， model group and ginseng intervention group. Two groups of experiments were conducted independently， and the experimental period was 5 weeks. At the end of the experiment， body weight， ethology， neuron structure/function index (NF-L， SYP)， astrocyte biomarker index (GFAP) and excitatory amino acid transport function index (EAATs) were measured and analyzed statistically. RESULTS The expression of astrocyte marker protein was highly expressed in the hippocampus of mice 1 to 3 weeks after corticosterone injection. The expression level was down-regulated at the 3rd week and showed a significant difference (P<0.05)， and the expression of neuronal structural function index was down-regulated. Neuronal damage， experimental animals showed significant changes in body weight and behavior (P<0.05). Further detection of astrocyte excitatory amino acid transporter protein showed down-regulation of expression and a positive correlation with neuronal damage.CONCLUSION Intraperitoneal injection of corticosterone can damage the hippocampus neurons of mice and exhibit behavioral abnormalities. The mechanism may be caused by stress affecting the astrocyte excitatory amino acid transport function.
Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells
LYUXing, LYUGao-hong, YEGuan
2019, 35(6): 687-690.
[Abstract](546) [PDF 1479KB](516)
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OBJECTIVE To investigate the mechanism of Xiangshenke formula in regulating the proliferation， migration and EMT of human gastric carcinoma cells SGC-7901. METHODS SGC-7901 cells were cultured in vitro， and then different concentrations of Xiangshenke formula containing serum were added into the cells. Wound healing test and transwell chamber assay were used to observe the SGC-7901 cell migration and invasion. MTT assay was used to investigate the SGC-7901 cell proliferation. Western blot was used to detect the expression levels of N-cadherin， Vimentin， E-cadherin， p-GSK3β/GSK3β and β-Catenin. RESULTS Xiangshenke formula effectively inhibited the migration and invasion of SGC-7901 cells， suppressed the cell proliferation， down-regulated the expression of N-cadherin， Vimentin， p-GSK3β/GSK3β and β-Catenin， and up-regulated the expression of E-cadherin. CONCLUSION Xiangshenke formula can inhibit the SGC-7901 cell epithelial-mesenchymal transition by regulating the GSK3β/β-Catenin signaling pathway， and suppress their migrtaion and invasion ability. It plays an important role in adjuvant therapy after gastric cancer surgery.
Mechanical Research of Chronic Oxidative Stress on the Activity of Breast Cancer Cells MCF-7 by Activating GSK3β/β-catenin Signaling Pathway
WANGJiang-tao, CHENXu, YAOChang, LIUXiao-an
2019, 35(6): 691-698.
[Abstract](544) [PDF 1842KB](463)
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OBJECTIVE To establish a oxidative stress cell line model of breast cancer cells MCF-7， observe the growth under this environment， and explore the effects of chronic stress status on breast cancer proliferation， migration and invasion of breast cancer and its possible mechanisms. METHODS The CCK-8 assay was used to detect the proliferation inhibition rate of MCF-7 under the conditions of acute oxidative stress and chronic oxidative stress. The ability of MCF-7 cells to migrate and invade under chronic oxidative stress conditions was examined by scratch assay and transwell assay. The level of IL-6 was determined by ELISA. Superoxide dismutase activity (SOD) and total antioxidant capacity (T-AOC) of MCF-7 under chronic oxidative stress model conditions were tested by kit. The expression levels of GSK3β/β-catenin signaling pathway-related proteins and RNA were determined by RT-PCR and Western blot. RESULTS Compared with acute oxidative stress， under the condition of chronic oxidative stress， the value-adding ability of MCF-7 was enhanced， and the ability of SOD and T-AOC was further enhanced. Its ability to migrate and invade is stronger than that of wild-type and MCF-7 under acute oxidative stress. This result is consistent with IL-6 protein expression， GSK3β/β-catenin signaling pathway-associated protein and RNA expression. It was also found that Sanhuang Decoction can inhibit the proliferation of MCF-7 chronic oxidative stress cells. CONCLUSION Chronic oxidative stress can promote the proliferation and metastasis of breast cancer cells MCF-7， and its mechanism may be related to the activation of GSK3β/β-catenin signaling pathway and the increase of IL-6 expression.
Reversal Effect and Mechanism of Alopecurone B on MCF-7 Adriamycin-Resistant Subline
HUANGLi-li, WANGXin, LIBing-bing, MIAOMing-xing
2019, 35(6): 699-703.
[Abstract](587) [PDF 1415KB](443)
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OBJECTIVE To study the reversal effect and the mechanism of APB on adriamycin (ADR)-induced multidrug-resistant human breast cancer MCF-7/ADR cells. METHODS ADR-resistant MCF-7 cells were maintained in RPMI-1640 culture medium containing 10%FBS and 250 ng/mL ADR. MTT assay， qPCR， Western blot and flow cytometry were used to determine the inhibitory effect of APB on P-gp levels and function in MCF-7 or MCF-7/ADR cells. RESULTS APB reversed MCF-7/ADR multidrug resistance at the concentration of 10 μmol/L. Treated with 0， 5， 10， 20 μmol/L of ABP， P-gp gene and protein levels were suppressed significantly in a concentration-dependent manner. Furthermore， APB in 10 μmol/L markedly inhibited the function of P-gp. CONCLUSION APB potent reversed the multidrug resistance in MCF-7 ADR-resistant subline. The mechanism referred to P-gp inhibition.
Effect of Xifeng Huashi Recipe on Visceral Hypersensitivity in Rats with Diarrhea Predominant Irritable Bowel Syndrome
LIHui, TIANYao-zhou, ZHANGXun-bing, GULi-mei, PANCheng-yu, ZHUFang-shi
2019, 35(6): 704-707.
[Abstract](509) [PDF 1344KB](562)
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OBJECTIVE To investigate the effect of Xifeng Huashi prescription (XFHS) on visceral hypersensitivity and SCF-Kit system in rats with diarrhea predominant irritable bowel syndrome (IBS-D). METHODS Rats were randomly divided into control group，model group， XFHS low， moderate and high dosage groups. The model was induced by separation of breast milk and coloclysis of acetic acid. High， moderate and low dosage XFHS group rats were given by gavage with different concentration of XFHS decoction，respectively. Equal saline was administrated in the normal group and model group. The abdominal withdrawal reflex was recorded， and the levels of SCF and c-Kit in the colonic tissue were detected by Western blot and qPCR. RESULTS Comparing with normal group， the grades of AWR and the levels of gastric SCF and c-Kit expression in the model group were significantly lower (P<0.05). The grades of AWR and the levels of gastric SCF and c-Kit expression in XFHS groups were significantly lower than those in the model group (P<0.05). CONCLUSION XFHS can reduce visceral hypersensitivity by inhibiting the expression of SCF-Kit system.
Study on the Mechanism of Circular-Icaritin Against Glucocorticoid-Induced Osteoporosis
WANG Hua-jun, QIAO Chen, ZHAO Rong, YIN Jiang-ning
2019, 35(6): 708-713.
[Abstract](506) [PDF 1664KB](515)
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OBJECTIVE To elucidate the reversal effect of cyclic-icaritin(CICT) on prednisolone -induced osteoporosis， and to explore its mechanism of action with BMPs signaling pathway. METHODS Prednisolone-induced osteoporosis model of zebrafish was used. The stained area， cumulative optical density， calcium and phosphorus contents of the skull of zebrafish were observed by fluorescence inverted microscope (×100). qPCR was used to quantitatively detect the expression of Runx 2 and AKP genes. RESULTS Compared with the control group， the cumulative optical density and mineralized area of zebrafish skull significantly decreased (P<0.01)，the Ca and P levels significantly decreased (P<0.01)， the expression of Runx 2 and AKP genes decreased significantly (P<0.01) after incubation with prednisolone (25 μmol/L).After the intervention with different doses of CICT (0.1， 1.0， 10.0 μmol/L)，the cumulative optical density and bone mineralization area of zebrafish skull significantly increased (P<0.01)， and the levels of Ca and P significantly increased (P<0.01). Molecular docking techniques showed that CICT could stably dock with target protein BMPs (BMP-2/BMP-4)， and the docking scores were - 5.49325609 and - 5.99361658， respectively. The expressions of Runx-2 and AKP genes significantly increased (P<0.01). CONCLUSION CICT can reverse glucocorticoid-induced osteoporosis， which plays an anti-osteoporosis role by binding CICT to BMPs protein target and regulating BMPs signaling pathway to promote osteogenic differentiation.
Investigation of Antitumor Activity of Brucea Javanica Dregs by RTCA and Determination of Brusatol and Bruceine A from Brucea Javanica Pieces and Dregs
YAN Guo-jun, HAN Lu, XIE Hui, SU Lian-lin, MA Xiao-fei, ZHANG Qian, LU Tu-lin, MAO Chun-qin
2019, 35(6): 714-718.
[Abstract](558) [PDF 1511KB](442)
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OBJECTIVE To investigate the antitumor activity of Brucea javanica pieces and the extracted dregs， and to compare the contents of Brusatol and Bruceine A. METHODS The inhibitory effect of Brucea javanica pieces and dregs on A549 cells was investigated by Real-time cell-based assay (RTCA)， and the contents of Brusatol and Bruceine A were determined by HPLC simultaneously. RESULTS The Brucea javanica pieces and dregs both showed certain inhibitory effects on A549 cells and the IC50 values were 4.576-7.256 mg/mL and 4.115-9.766 mg/mL. The total content of Brusatol and Bruceine A in Brucea javanica pieces and the extracted dregs were 0.113 9%-0.143 5% and 0.110 0%-0.135 9%. CONCLUSION The pieces and dregs of Brucea javanica have certain antitumor activity， and there is no significant difference in the contents of anti-tumor components between the dregs and the pieces. The dregs of Brucea javanica can be researched and developed in depth centering on nigakilactone. It also can promote the comprehensive use of Chinese medicinal resources.
Study on Acupoint Selection Rules of Treating Infertility in Ancient Literature
XIJin, JINChun-chun, SHENZhen-ru, SHENJie, XIAYou-bing
2019, 35(6): 719-723.
[Abstract](659) [PDF 1253KB](681)
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OBJECTIVE To explore of acupoint selection rules of treating infertility recorded in ancient literature. METHODS Chinese Medical Classics (5th Edition) was used to search and collate the records of treating infertility in the books written before the founding of the People's Republic of China. Based on the database， the authors made statistical analysis. RESULTS 301 clauses of acupuncture and moxibustion treatment on infertility were retrieved， referred to 41 points for 358 times. It has been found that acupoints on Ren channel and Kidney meridian were often used to treat infertility， among which Zhongji (RN3) and Guanyuan (RN4) were the most frequent. The points were mostly selected from the lower abdomen and lower limbs and most of them are specific. The convergent acupoint of Ren Channel and Chong Channel were key to the treatment. CONCLUSION In the treatment of infertility， we can select the most frequent prescribed acupoints in ancient literature as a basic prescription for acupuncture， such as Zhongji（RN3）， Guanyuan (RN4)， Zhaohai (KI6) and Yongquan (KI1)， combined with moxibustion and syndrome differentiation. In addition， it was recorded that acupuncture at Shimen (CV5)， Sanyinjiao (SP6)， Xuehai (SP10)， Zhiyang (GV9) might lead to worse condition， so acupoint selection to treat infertility should be deliberate.
Study on Therapy Characteristics and Medication Feature in Zhang Jian-zhai's Case Records
YELiang, SHANGEr-xin, HOUJun, ZHANGGong-yu, LINPu
2019, 35(6): 724-727.
[Abstract](631) [PDF 1107KB](606)
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OBJECTIVE To discuss Zhang Jian-zhai's therapy characteristics and medication feature in the treatment of miscellaneous disease due to internal injury based on Zhang Jian-zhai's Case Records. METHODS The clauses of therapy for internal diseases in Zhang Jian-zhai's Case Records was summarized. Then APRIORI association rules and binomial test were applied to conduct data mining， analysis on those therapeutic methods so as to disclose Zhang's medication feature. RESULTS On the summary of the therapy recorded in Zhang Jian-zhai's Case Records， it has been found that there were four most commonly applied therapeutic methods， that is， dredging， harmonizing， clearing and strengthening， which applied Chinese medicine with relevant properties. It also showed us the most commonly prescribed Chinese medicine， like gan cao (glycyrrhiza uralensis)， bai shao (paeoniae alba)， ban xia (pinelliae tuber) and fu shen (sclerotium poriae pararadicis). As for the highly used couplet medicinals and formulae， they were mainly prescribed based on the combination of these four herbs. CONCLUSION In terms of the four commonly applied therapeutic methods to treat miscellaneous disease due to internal injury， dredging method was to release the exterior pathogen， dispel the inner dampness， rectify qi and harmonize stomach so as to resolve the obstruction. Harmonizing method highlighted the role of calming mind in addition to reconciling yin and yang. Clearing method was to clear lung heat， dissolve phlegm and calm heart. Strengthening method was to diffuse the exterior by nourishing the interior. On the whole， Mr Zhang's medication feature tended to be mild， which aimed at regulating zang-fu organs. Furthermore， fu shen was applied in three of the four therapeutic methods， which implicated Zhang's clinical thinking of emphasizing the regulation of heart spirit.
Professor Zou Yan-qin's Clinical Experience in Treating Kidney Cancer
ZHONG Yu
2019, 35(6): 728-731.
[Abstract](421) [PDF 1101KB](465)
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The paper summarized the clinical experience and treatment ideas of professor Zou Yan-qin， a national Chinese medical science master， in the treatment of kidney cancer. It held that the treatment of kidney cancer should maintain the nephron， harmonize and yang， recuperate five zang organs， suppress tumor and detoxification， promote blood circulation and remove phlegm to soften hard masses， promote defecation to dispel pathogenic factors. By maintaining the congenital and acquired foundation， adjusting zang-fu organs， blood and qi， yin and yang， and eliminating cancer，it's aimed to strengthen healthy qi and eliminate pathogens.
Analysis on Professor Han Ming-xiang's Experience in the Treatment of Cough Variant Asthma
CHENJing-jing, ZHANGNian-zhi
2019, 35(6): 732-734.
[Abstract](508) [PDF 1088KB](495)
Abstract:
The article summarized professor Han Ming-xiang's clinical experience in the treatment of cough variant asthma (CVA). Professor Han held that CVA is closely related to wind， phlegm， stasis and deficiency. In terms of the etiology and pathogenesis of CVA， he put forward "One root cause and three symptoms"， that is， deficiency of qi and yin as "one root cause"， and the disturbance of wind pathogens， the retention of phlegm and fluid retention and the accumulation of stasis are "three symptoms". The treatment principle is guided by expelling wind and dispersing lung， moistening pharynx and throat， resolving phlegm and dredging collaterals， activating blood circulation and removing blood stasis as the main line， invigorating spleen and qi and nourishing yin and increasing fluid as the basis， in order to achieve the purpose of strengthening healthy qi， eliminating pathogenic factors and treating both the root cause and symptoms.
Professor Zhang Shi-qing's Experience on Infantile Anorexia with Paired Herbs
LIYu-xia, SHIZheng-gang, WULi-ping
2019, 35(6): 735-737.
[Abstract](418) [PDF 1093KB](529)
Abstract:
This paper summarized commonly used paired herbs to treat infantile anorexia by professor Zhang Shi-qing， including cang zhu (atractylodes rhizome) paired with bai zhu (atractylodes macrocephala)， wu mei (fructus mume) paired with mu gua (chaenomeles fruit)， qing pi (green tangerine peel) paired with chen pi (tangerine peel)， and jiao san xian (stir-baking fructus hordei germinatus et crataeggi et massa fer-mentata medicinalis) paired with ji nei jin (endothelium corneum gigeriae galli). Combined with modern pharmacological research， the authors had analyzed compatible characteristics of prescriptions and professor Zhang's clinical experience and further explicated his academic thoughts so as to better provide guidance for clinical practice.
Correlation Between Traditional Chinese Medicine Syndrome and Clinical Biochemical Indexes of Non-Alcoholic Fatty Liver
GULi-mei, CAOPei-rang, GUChao, WEILan-fu, TIANYao-zhou
2019, 35(6): 738-740.
[Abstract](542) [PDF 1101KB](475)
Abstract:
OBJECTIVE To explore the distribution law， characteristics and clinical factors of traditional Chinese medicine (TCM) syndrome types in patients with non-alcoholic fatty liver disease (NAFLD). METHODS 249 patients with NAFLD were enrolled in the study， and their syndrome differentiation was divided into five types: damp-turbidity stagnation syndrome， liver depression and spleen deficiency syndrome， accumulation of damp-heat syndrome， obstruction of phlegm and stasis syndrome and spleen-kidney deficiency syndrome. To explore the correlation between TCM syndrome types， age， gender， BMI and blood purification indicators. RESULTS ①Proportion of syndrome types: damp-turbidity stagnation syndrome accounting for 30.1%， 28.1% for liver depression and spleen deficiency syndrome， accumulation of damp-heat syndrome 25.7%， obstruction of phlegm and stasis syndrome 8.8%， spleen-kidney deficiency syndrome 7.2%.②The ratio of male to female was about 1.39∶1. The patients with accumulation of damp-heat syndrome and damp-turbidity stagnation syndrome were mainly male (P<0.05); those with liver depression and spleen deficiency syndrome were mainly female (P<0.01). ③The alanine transaminase (ALT) elevation in the accumulation of damp heat syndrome was the most significant， and there was statistical difference compared with that of patients with damp-turbidity stagnation syndrome and the liver depression and spleen deficiency syndrome group (P<0.05). The total cholesterol (TC) elevation was the most significant in the damp-turbidity stagnation syndrome， and there was a statistical difference compared with the liver depression and spleen deficiency syndrome， accumulation of damp-heat syndrome， and spleen-kidney deficiency syndrome group (P<0.05). CONCLUSION The most common TCM syndrome types of NAFLD are damp-turbidity stagnation syndrome， liver depression and spleen deficiency syndrome and accumulation of damp-heat syndrome. The male incidence rate is higher than that of female; accumulation of damp-heat syndrome is more liable to induce ALT injury; damp-turbidity stagnation syndrome is more likely to cause TC elevation.
Effect of Tangbikang on Cytokines in Diabetic Rats
LYUCui-yan, MAOYing-qiu, ZHANGZhu-hua, LIUTong-hua
2019, 35(6): 741-743.
[Abstract](532) [PDF 1168KB](415)
Abstract:
OBJECTIVE To observe the traditional Chinese medicine compound Tangbikang in diabetic rats tumor necrosis factor (TNF-α)， monocyte chemotactic protein (MCP-1)， vascular cell adhesion molecule 1 (VCAM 1)， blood oxidized low density lipoprotein (oxLDL) and intercellular adhesion molecule 1 (ICAM - l) expression. METHODS SD rats were fed with high-fat diet， injected with STZ to induce type 2 diabetes， and 16 weeks after intervention with of Tangbikang， abdominal aortic blood and samples of one side of the sciatic nerve of the rats were collected， serum cytokine levels were detected by ELISE method， and expressions of MCP-1，VCAM-1 and TNF-α in sciatic nerve tissues were detected by Western blot. RESULTS Compared with the normal group， the expression of TNF-α， VCAM-1 and MCP-1 in the sciatic nerve tissues of the model group and the content of oxLDL， ICAM-1， VCAM-1， TNF-α and MCP-1 in the serum of the model group were significantly increased (P<0.01). Compared with the model group， Tangbikang significantly reduced the expression of cytokines in tissues and serum (P<0.05，P<0.01) in a dose-dependent way. CONCLUSION Tangbikang can down-regulate the contents of serum TNF-α， oxLDL， ICAM-1，VCAM-1 and MCP-1 in STZ-induced diabetic rats and the expression of VCAM-1， TNF-α and MCP-1 in the sciatic nerve， which may be one of the mechanisms of Tangbikang's neuroprotection in diabetic peripheral neuropathy.
Thinking and Analysis on the Safety of Traditional Chinese Medicine Injection
PENG Guo-ping, LI Cun-yu
2019, 35(6): 744-751.
[Abstract](831) [PDF 1164KB](465)
Abstract:
The safety of traditional Chinese medicine injection (TCMI) has become the hot question in the society. Correct understanding of the adverse reactions of TCMI can effectively avoid the misunderstanding or misperception among the public. At present， the raw materials， production process and quality control were the key points which influence the safety of TCMI injections. According to the clinical problems of various varieties， it should concentrate on strengthening basic research， standardization the instructions and drug compatibility， enhance the safety control of the related substances， insoluble particles， pyrogen and allergens to ensure the safe usage of TCMI.
Meta Analysis of Treatment of Coronary Heart Disease Angina with Shenmai Injection and Danshen Injection
TIAN Lei, ZHAO Xin-rui, MA Ai-xia
2019, 35(6): 752-756.
[Abstract](520) [PDF 1550KB](539)
Abstract:
OBJECTIVE To systematically evaluate the clinical effect of Danshen injection and Shenmai injection in the treatment of coronary heart disease. METHODS Searching the Cochrane Library， Wanfang data resource system， Medline database， full text Database of Chinese Sci-tech Journals (VIP) and the Database of China Knowledge Network (CNKI)， all randomized controlled clinical trials of Danshen injection (control group) and Shenmai injection (experimental group) in the treatment of coronary heart disease were included. The literature screening， data extraction and quality evaluation were carried out according to Cochrane Handbook evaluation standard. RevMan 5.3 software was used to carry on the Meta analysis to the included research. RESULTS A total of 1 211 articles were searched and 20 RCTs were included in this study， involving 2 034 patients. Meta-analysis showed that Shenmai injection group significantly improved the clinical symptoms and ECG manifestations of coronary heart disease (RR=1.22， 95%CI:1.16~1.28， I2=0%， P≤0.80) compared with Danshen injection group. No adverse reactions were reported. CONCLUSION Shenmai Injection is more effective than Danshen injection in the treatment of coronary heart disease.
Advances in Pharmacological Effect and Q-marker Research of Penthorum Chinese<\i>
LIJie, JIANGZhi-tao, LIUXiao-yan, DILiu-qing, LIUChun-di
2019, 35(6): 757-760.
[Abstract](856) [PDF 1201KB](576)
Abstract:
As a traditional Miao medicine， Penthorum Chinese has a long history of use in the folk society. Modern pharmacological studies have shown that Penthorum Chinese has antioxidant， anti-hepatitis virus， protection against alcohol/non-alcoholic liver damage and choleretic effects. Through modern analysis techniques， it has been found that the herb contains a variety of chemical components， including flavonoids， lignin， coumarin， acetophenone， tannins and triterpenes. This paper reviews the active ingredients and pharmacological properties of Penthorum Chinese， then studies the relationship between them， and preliminary establish penchinone B as the Q-marker， which provide reference for the resource development and utilization of Penthorum Chinese.
Research Progress on Traditional Chinese Medicine in the Treatment of Alzheimer's Disease
QIAN Ying, SHAO Yu-rou, LU Sheng-feng, WANG Jing-jing, CHEN Zhi-peng
2019, 35(6): 761-766.
[Abstract](1346) [PDF 1223KB](712)
Abstract:
Traditional Chinese medicine has made certain progress in the treatment of Alzheimer's disease in recent years. Acupuncture， Chinese medicine formula and combination treatment of acupuncture and medicine are main methods in the treatment of Alzheimer's disease. In this paper， the characteristics of clinical application and the mechanism of these treatments are summarized. Some new ideas are prospected and expected to provide new methods for the treatment of Alzheimer's disease.
Research Progress of Traditional Chinese Medicine in the Treatment of Esophageal Cancer After Operation
ZHAYong-qiang, SUNDong-dong, PANLi-qun
2019, 35(6): 767-770.
[Abstract](705) [PDF 207KB](1082)
Abstract:
The incidence and mortality of esophageal cancer are high. Surgery， radiotherapy and chemotherapy are the main methods of esophageal cancer. Traditional Chinese medicine (TCM) has the advantages of improving symptoms， synergism and detoxification， improving immunity and quality of life of patients with esophageal cancer in terms of postoperative complications and toxic side effects caused by radiotherapy and chemotherapy. This paper reviews the understanding and treatment of esophageal cancer in traditional Chinese and Western medicine， focusing on the research progress in the treatment of postoperative complications， postoperative comprehensive treatment and postoperative rehabilitation of esophageal cancer， in order to provide a reference for the clinical treatment of esophageal cancer.

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