南京中医药大学学报

The Characteristics of Wumei Pill in Treatment Based on the Recovery of Disease from Jueyin

SHI Suo-fang

2019, 35(2): 121-123.

[Abstract](1056) [PDF 1061KB](1450)

Abstract:
This paper expounds the Jueyin disease in Treatise on Cold Diseases from the perspective of the three yin three yang and kai-he-shu theory in The Yellow Emperor's Inner Classic， and probes into application of Wumei pill when treating the Jueyin disease. It is believed that Jueyin is the time when Yin is running out while Yang is about to rise. If Yin is exhausted or Yang cannot be transformed normally， the problem of incongruity between Yin and Yang will arise， which will lead to the Jueyin disease. If the onset time of a disease accords with the Jueyin， Wumei pill can be used for treatment.

Study on the Pathogenesis， Syndrome and Treatment of Vexing Pain in Treatise on Cold Damage and Essentials from the Golden Cabinet

LI Xin, WANG Shi-yuan

2019, 35(2): 124-126.

[Abstract](742) [PDF 1055KB](892)

Abstract:
Discriminating the symptoms of vexing pain in Treatise on Cold Damage and Essentials from the Golden Cabinet， it was suggested that the pathogen of vexing painwas constraint of yang qi， either caused by wind-cold-dampness fettering the exterior， or sthenia of yang qi and cold blocking the exterior， or shaoyang inability to pivot， or interior heat accumulation caused by excessive yang heat， or steaming dampness-heat resulting in blockage of yang， or internal obstruction of cold-turbidity leading to failure of dispelling yang qi. The treatment should be based on specific causes and pathogenesis-constraint of yang qi. The treatment principle is dissipating excessive stagnation of fire.

Differentiation and Analysis of Afternoon Fever

BU Huan-yu

2019, 35(2): 127-129.

[Abstract](716) [PDF 1058KB](1738)

Abstract:
Afternoon fever is the most common type of fever， but the misdiagnosis was easily made. The paper summarized the types of afternoon fever mentioned in Treatise on Cold Damage and Miscellaneous Diseases， Systematic Differentiation of Warm Diseases and some other related classics. The paper holds that afternoon fever caused by excess of the viscera， dampness and fever， blood stasis and other pathogenic factors results in qi stagnation， which was sthenic fever. Dispelling pathogen and diffusing qi is favored in treating the sthenic afternoon fever. While the treatment of asthenic afternoon fever， based on the detailed differentiation of the deficiency and damage of viscera should be primarily nourishing yin， and concurrently dispelling pathogen.

A Multicenter， Randomized， Double-Blind Clinical Study of Huoxue Tongfu Formula in Postoperative Peritoneal Adhesions

YANGLi-li, BIANYao-yao, QIANHai-hua, ZHUJian-guang, XUSu, CHENHao, WANGSen, LIWen-lin, ZENGLi

2019, 35(2): 130-134.

[Abstract](740) [PDF 1107KB](556)

Abstract:
OBJECTIVE To observe the clinical effect of Huoxue Tongfu formula on prevention and treatment of postoperative peritoneal adhesions (PPA) and to explore its possible mechanism. METHODS 119 patients with adhesive intestinal obstruction were divided into two groups: control group (n=58) and treatment group (n=61). The control group was treated with routine therapy and the treatment group was treated with Huoxue Tongfu formula on the basis of the control group. After 4 weeks of intervention， the first time of exhaust and defecation were observed in the two groups， and the VAS score of postoperative abdominal pain was also observed. Serum transforming growth factor -β (TGF-β)， the level of fibronectin (FN) and the B-ultrasound adhesion after operation were evaluated， and the recurrence rate of the adhesive ileus and rate of its recurrent operation were observed at 1-year follow-up. RESULTS Compared with the control group， the treatment group reduced the first time of exhaust and defecation after operation (P<0.01)， lower the VAS pain score of 7 d and 14 d (P<0.01)， reduce the postoperative peritoneal adhesion formation (P<0.05)， and reduce recurrence rate of postoperative adhesive intestinal obstruction and the rate of its recurrent operation (P<0.05). Comparing with postoperative 3th day， there was statistically significant on postoperative 7th day and 14th day(P<0.05， P<0.01) in control group， and there was significant difference on postoperative 7th day and 14th day(P<0.01) in treatment group. The serum level of TGF-β and FN were significantly increased on postoperative 3th day(P<0.01) both in treatment group and control group. And there was no significant difference on postoperative 14th day(P>0.05) in treatment group. Comparing the two groups， there was a significant reduction in treatment group on postoperative 7th and 14th day(P<0.01). CONCLUSION The effect of Huoxue Tongfu formula on postoperative peritoneal adhesion is significant， and it is worthy of clinical popularization and application.

Intervention of Different Doses of Huangqi on Immune Function and Platelet Activating Factor in Patients with Sepsis

SHEN Li-juan, WU Xi-ping, GUAN Yun-yan, SUN Yue-wen, WANG Qian

2019, 35(2): 135-138.

[Abstract](709) [PDF 1090KB](494)

Abstract:
OBJECTIVE To observe the effects of different doses of Huangqi on immune function and platelet activating factor in patients with sepsis， and to explore the mechanism of Huangqi in improving immune status of sepsis patients. METHODS 60 patients with sepsis admitted to the Department of Severe Medicine， Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine were randomly divided into three groups: conventional treatment group (20 cases， conventional western medicine treatment)， small dose of Huangqi group (20 cases， 1.5 g of Huangqi granules added on the basis of conventional western medicine treatment， once a day)， medium dose of Huangqi group (20 cases， 3.0 g of Huangqi granules added on the basis of conventional western medicine treatment， once a day)， 5 d a course. The Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score， sequential organ failure score (SOFA)， peripheral blood lymphocyte subsets， platelet activating factor (PAF) level and 28-day mortality of the three groups before and after treatment were compared. RESULTS The number of total T cells， T helper lymphocyte， CD4⁺/CD8⁺， and B lymphocyte increased after treatment in different doses of Huangqi group (P<0.05， P<0.01)， while T-suppressed lymphocyte and NK cell decreased (P<0.05， P<0.01)， the curative effect was better than that in conventional treatment group (P<0.05)， and the difference was more significant in middle dosage group than small dosage group (P<0.05). The levels of PAF in the three groups decreased after treatment with statistical significance (P<0.05)， while those in the Huangqi groups were better than those in the conventional group (P<0.05). The decrease of PAF in the middle dose group was more obvious than that in the small dose group， and the difference was statistically significant (P<0.05). The APACHE Ⅱ score and SOFA score of the three groups were significantly lower than those before treatment (P<0.05)， while the Huangqi groups were better than the conventional group (P<0.05). The 28-day mortality rate in the middle dose and small dose groups were lower than that in the conventional group， and the difference was significant (P<0.05) but there was no difference between the two groups (P>0.05). CONCLUSION Huangqi granules can improve immune system and mortality of sepsis patients in a dose-dependent manner. Its mechanism may be accomplished by regulating PAF.

Effects of TCM Syndrome Differentiation Combined with Adefovir Dimethoate on Liver Function， Liver Fibrosis and Inflammatory Reaction in Patients with Chronic Hepatitis B

YI Ting, ZHENG Ting-ting, LIU Jing-han, YAN Tong

2019, 35(2): 139-143.

[Abstract](660) [PDF 1104KB](494)

Abstract:
OBJECTIVE To investigate the effects of TCM syndrome differentiation combined with adefovir dimethoate on liver function， liver fibrosis and inflammatory reaction in patients with chronic hepatitis B (CHB). METHODS 164 patients with chronic hepatitis B in our hospital from December 2010 to December 2017 were divided into two groups， 82 patients in each group. The control group were treated with adefovir dimethoate orally， while the treatment group were treated with TCM syndrome differentiation on the basis of oral adefovir dipivoxil， the course of treatment for 3 months. The changes of liver function， liver fibrosis and inflammatory reaction of two groups were compared. RESULTS The obvious efficiency， effective rate and reduction of TCM syndrome score after treatment of the treatment group were significantly improved compared with those before treatment (P<0.05，P<0.01). The improvement of liver function in treatment group was significantly better than that of the control group and significantly improved than that before treatment(P<0.01). HBV-DNA negative conversion rate was significantly higher than that of the control group and significantly improved than that before treatment (P<0.05). Hyaluronic acid (HA)， type Ⅳ collagen (Ⅳ-C) and laminin (LN) were significantly lower in the treatment group than in the control group and significantly improved than that before treatment (P<0.01). The content of transforming growth factor(TGF-β1)， the hypersensitive C-reactive protein (hs-CRP)， tumor necrosis factor-α (TNF-α) were significantly decreased than that of the control group， and are obviously improved before the treatment. There was statistical significance(P<0.01). CONCLUSION The combination of the syndrome differentiation and adefovir dipivoxil in the treatment of chronic hepatitis B can significantly improve the liver function， liver fibrosis and inflammation of the body.

The Effect of Yiqi Yangyin Tongluo Formula on Micro-Inflammatory State of Patients with Early Diabetic Nephropathy

DOUChen-hui, CHENXiao-hu, PENGShu-ling

2019, 35(2): 144-147.

[Abstract](782) [PDF 1087KB](523)

Abstract:
OBJECTIVE To explore the effect of Yiqi Yangyin Tongluo formula on micro inflammatory state of patients with early diabetic nephropathy. METHODS 78 patients were randomly divided into two groups with 39 patients in each group. The control group was given conventional basic treatment， and the treatment group was treated with Yiqi Yangyin Tongluo formula on the basis of the control group. After 12 weeks， the changes of C-reactive protein (CRP)， tumor necrosis factor (TNF-α)， urinary albumin excretion rate (UAE) and clinical efficacy， TCM syndrome score， blood pressure， blood glucose， urea nitrogen (BUN)， creatinine (Scr) and other indicators in two groups were compared. RESULTS The total effective rate and TCM syndrome score improvement in the treatment group were better than those in the control group (P<0.05). Compared with pre-treatment， the levels of UAE， CRP and TNF-α in the two groups were lower (P<0.05， P<0.01)， and the improvement in the treatment group was better than that in the control group (P<0.05). No renal deterioration appeared during the observation process. CONCLUSION Yiqi Yangyin Tongluo formula can improve the symptoms of patients with early diabetic nephropathy， reduce the levels of inflammatory factors CRP， TNF-α， reduce proteinuria， and significantly interfere with the micro inflammation of early diabetic nephropathy， thus delaying the progression of diabetic nephropathy.

Clinical Observation of Arthroscopic Debridement Combined with Xianfang Huoming Decoction in Treating Acute Suppurative Arthritis

HUJun, ZHOUZhong

2019, 35(2): 148-151.

[Abstract](744) [PDF 1088KB](569)

Abstract:
OBJECTIVE To observe the clinical efficacy of arthroscopic debridement combined with Xianfang Huoming Decoction in treating acute suppurative arthritis. METHODS 68 patients with acute suppurative arthritis during the hospitalization of Lianyungang TCM Hospital Affiliated to Nanjing University of Chinese Medicine and Integrated Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine， from March 2014 to December 2017， were randomly divided into 2 groups， 34 patients in each group. All the 68 patients were under the arthroscopic debridement treatment. The patients were lavaged and drainaged with gentamicin-containing physiological saline (80 000 U/500 mL) for 1 to 2 weeks after the operation. According to the drug sensitivity test， the sensitive antibiotics were selected for intravenous infusion for 2 weeks， and the treatment group was treated with Xianfang Huoming Decoction. The control group was only under the arthroscopic debridement treatment. Three weeks after the operation， observe the Lysholm score， pain visual analogue scale (VAS)， Judet knee flexion and functional evaluation of two groups before and after treatment. RESULTS After treatment， the Lysholm knee joint score of the treatment group was significantly higher than that of the control group (P<0.05). The pain visual analog score (VAS) of the treatment group was significantly lower than the control group (P<0.05). Judet knee flexion and function evaluation excellent rate of the treatment group (91.18%)， the excellent rate of the control group (70.59%). The treatment group was significantly higher than the control group (P<0.05). No significant adverse reactions were observed in the two groups. CONCLUSION Arthroscopic debridement combined with Xianfang Huoming Decoction can significantly shorten the treatment course of suppurative knee arthritis， rapid recovery of joint function and high rate of excellent， which is an ideal treatment.

Clinical Effect of Traditional Chinese Spinal Orthopedic Manipulation in Treatment of Knee Osteoarthritis

QULiu-xin, WANGLu-ye, XINGLi-yang, GAOSong, LIPing, KEGuang-juan

2019, 35(2): 152-155.

[Abstract](684) [PDF 1087KB](671)

Abstract:
OBJECTIVE To observe the clinical effect of Traditional Chinese Spinal Orthopedic Manipulation (TCSOM) in treating knee osteoarthritis (KOA) in comparison with Celecoxib Capsules (CC)， and to assess a possible cause for KOA. METHODS 80 cases of KOA patients in department of Orthopaedic Surgery， Zhongda Hospital Affiliated to Southeast University form Nov. 2016 to Oct. 2017 were randomly divided into two groups: treatment group and control group， 40 cases in each group. The treatment group was treated by TCSOM， while the control group was treated by CC orally， 200mg each time， once a day. Magnetic resonance examination and floating patellar test were performed on the knee before and 4 weeks after treatment. WOMAC score was used to evaluate KOA before treatment， 4 weeks after treatment and 3 months after treatment， and the improvement rate of osteoarthritis severity index (ISOA) was used to judge the curative effect. RESULTS The degree of synovitis， joint effusion and WOMAC score of KOA in the two groups were significantly improved (P<0.01)， and the improvement in the treatment group was significant (P<0.01). No obvious adverse reactions were found in both groups. CONCLUSION It is a simple and effective method to treat KOA from waist with TCSOM. Displacement of upper lumbar intervertebral disc may be the cause of KOA.

Study on Diuretic Effects and Its Mechanism of Phytolaccae Radix Before and After Vinegar Processing

CHENGZi-ye, YUHong-li, WUHao, WANGWei, ZHANGYuan-bin, SHANXue-lian, CHENYun, LIShu-hui, CAOJing

2019, 35(2): 156-159.

[Abstract](673) [PDF 1462KB](586)

Abstract:
OBJECTIVE To investigate the effects of phytolaccae radix before and after vinegar processing on diuretic effects and related factors. METHODS Saline-loaded model rats were given different kinds of phytolaccae radix decoction by gavage to detect the changes of urine volume within 5 h， plasma concentrations of ADH， ALD， ANP and protein expression levels of AQP2， AQP3 and AQP4. RESULTS Phytolaccae radix before and after vinegar processing significantly increased the urine volumes of rats (P<0.01) and vinegar product significantly increased urine volume compared to raw product (P<0.05). Only high dose of raw product significantly reduced plasma concentration of ADH (P<0.01) and caused a tendency to reduce ALD and increase ANP content， without significant difference. Vinegar product significantly reduced plasma concentrations of ADH， ALD and increased ANP content (P<0.05， P<0.01). Raw product significantly inhibited the protein expressions of AQP2， AQP3 and AQP4 (P<0.05， P<0.01) while vinegar product significantly inhibited the protein expressions of AQP2 and AQP3 (P<0.01)， whereas showed no significant influence on AQP4 protein expression， compared to control group. Moreover， vinegar product inhibited the protein expression of AQP3 more significantly than raw product (P<0.05). CONCLUSION Phytolaccae radix after vinegar processing still retains diuretic effect and its efficacy is better than that of raw product at the molecular levels. The diuretic mechanism of phytolaccae radix maybe associated with changing hormone levels of fluid metabolism， and the inhibition of AQPs protein expressions in renal distal convoluted tubules and collecting ducts， which can inhibit water reabsorption.

Study on the Anti-Melanoma Effect of Shiquan Dabu Decoction and its Combination Therapy with Cisplatin

ZHANGTing-ting, ZOUWei, YANGChun-mei, QIANCheng, WUYuan-yuan, LIXiao-man, WANGAi-yunMZ)〗

2019, 35(2): 160-165.

[Abstract](757) [PDF 1953KB](555)

Abstract:
OBJECTIVE To investigate the anti-tumor effect and mechanism of Shiquan Dabu Decoction （SQDB）on mouse melanoma， and to elucidate the effect and administration of the combination with cisplatin（DDP）. METHODS Flow cytometry was used to investigate the effect of SQDB on cell cycle and apoptosis of melanoma cell line B16F10. Mouse spleen cells were isolated and MTS assay was used to investigate the anti-proliferative activity of SQDB in spleen cells induced by ConA or LPS. The model of mouse melanoma subcutaneous xenograft was established. The anti-tumor effect of SQDB was detected by HE staining， immunohistochemistry and TUNEL staining. The melanoma metastasis model was constructed to study the potential mechanism on the inhibition of the tumor metastasis by SQDB. RESULTS 10 mg/mL SQDB could induce apoptosis of B16F10 melanoma cells; 2.5， 5， 10 mg/mL SQDB reduced the number of G₀/G₁ cells; 10 mg/mL SQDB increased the number of S phase cells; 2.5 mg/mL of SQDB increased the number of cells in G₂/M phase; 5% and 10% drug-containing serum promoted the proliferation of B lymphocytes induced by LPS; 5%， 10% and 15% drug-containing serum also promoted the proliferation of T lymphocytes caused by Con A. Using DDP and SQDB simultaneously or using DDP first and then SQDB inhibited the growth of melanoma. Using both DDP and SQDB significantly inhibited tumor cell proliferation and promoted tumor cell apoptosis; When DDP and SQDB were used together， or when DDP was used first， SQDB could significantly inhibit melanoma lung metastasis. In addition， the thymus and spleen coefficient of the mice decreased significantly when DDP alone was used (P<0.05， P<0.01)， and the simultaneous administration of SQDB and DDP could increase the thymus coefficient of the mice (P<0.05). CONCLUSION SQDB can inhibit the proliferation of melanoma B16F10， promote B16F10 apoptosis and the proliferation of mouse spleen lymphocytes. Combined with DDP， SQDB can enhance the anti-tumor effect of mouse xenografts and metastatic tumor models.

Effects of Liguzinediol on Hemodynamics in Chronic Heart Failure Rats Induced by Doxorubicin

TONGJing, ZHUQing, GUOYao, GUORui, ZHAOFeng-ming, LIWei, LIYu, BIANHui-min

2019, 35(2): 166-170.

[Abstract](642) [PDF 1724KB](688)

Abstract:
OBJECTIVE To explore the effect of Liguzinediol on cardiac function， ventricular remodeling and inflammation in chronic heart failure (CHF) rat model induced by doxorubicin (Dox). METHODS The hemodynamic changes in the maximum ascending/descending rate of left ventricular pressure (+dp/dt_max)， left ventricular pressure (LVSP)， arterial systolic pressure (ASP)， arterial diastolic pressure (ADP) and heart rate (HR) were recorded by multichannel physiological recorder with intragastric administration of Liguzinediol in CHF rats induced by Dox (intraperitoneal injection， 2 mg/kg). The effects of Liguzinediol on oxygen free radicals (NO， NOS， SOD， MDA) and inflammatory factors (IL-6 and TNF-α) were also detected by the kits. RESULTS Liguzinediol increased the levels of LVSP， +dp/dt_max， ASP， ADP， AP and HR， and decreased the level of -dp/dt_max(P<0.05，P<0.01). Liguzinediol decreased the concentration of NO， iNOS and MDA ， increased the activity of SOD， and inhibited the contents of IL-6 and TNF-α(P<0.05，P<0.01). CONCLUSION Liguzinediol can improve ventricular remodeling by reducing the release of inflammatory factors， inhibiting the formation of oxygen free radicals.

Chemopreventive Effect of Quercetin on Oral Squamous Cell Carcinoma by Regulating NF-κB Signaling Pathway

ZHANGWen, FANYuan, ZHENGYang-yu, BIANYong, DAIJian-guo, YINGang

2019, 35(2): 171-174.

[Abstract](582) [PDF 1490KB](718)

Abstract:
OBJECTIVE To investigate the chemopreventive effect of quercetin on oral squamous cell carcinoma (OSCC) by regulating NF-κB signaling pathway. METHODS Golden hamster buccal pouch carcinoma model was established by DMBA Induction. The model was divided into 6 groups: blank control group， quercetin administration group， DMBA model group， low dose prevention group， medium dose prevention group， high dose prevention group， qPCR and Western Blot to detect NF-κB. The expressions of NF-κB-p50， NF-κB-p65， IκBα， p- IκBα and IKKβ were detected， and the chemical components of quercetin were docked with IKK beta kinase. Computer-aided virtual docking method was used to simulate the docking of quercetin with IKK beta kinase molecule. RESULTS Western blot analysis showed that the expression levels of NF-κB-p50， NF-κB-p65， p-IκBα and IKKβ in medium dose prevention group and high dose prevention group were significantly lower than those in DMBA model group， but the expression levels of IκBα were significantly higher than those in DMBA model group. qPCR results showed that the expression levels of NF-κB-p50 mRNA and NF-κB-p65 mRNA in high dose prevention group were significantly lower than those in DMBA model group. Molecular simulation docking results showed that quercetin docked with IKK beta kinase region in the same active chamber through hydrogen and PI bonds. CONCLUSION Quercetin may play a chemopreventive role in oral squamous cell carcinoma by targeting the NF-κB signaling pathway.

Influence on Ultrastructure of Colonic Mucosa in Colon， Expression of AQP3， AQP8 by Electroacupuncture at Different Acupoints Combination in Rats with Fuctional Constipation

WANGYuan, WANGQiang, LIUSi-yang, ZHOUFeng, NIUWen-min, LIUZhi-bin, LIULong

2019, 35(2): 175-179.

[Abstract](593) [PDF 1379KB](541)

Abstract:
OBJECTIVE To explore the mechanism of electroacupuncture at different points combination in the treatment of rats with functional constipation (FC). METHODS SD rats replicated by intragastric administration with 0 ℃ 0.9% sodium chloride solution were taken as FC animal model and randomly divided into model group， electroacupuncture group Ⅰ， electroacupuncture group Ⅱ and electroacupuncture group Ⅲ， SD rats were taken as control group. EAⅠhad electroacupuncture at bilateral Tianshu(ST 25)， Dachangshu(BL 25)， EAⅡ had electroacupuncture at bilateral Quchi(LI 11)， Shangjuxu(ST 37)， EAⅢ had electroacupuncture at unilateral Tianshu(ST 25)， Dachangshu(BL 25)， Quchi(LI 11)， Shangjuxu(ST 37). Observation on fecal characteristics and intestinal carbon propelling rate were used to evaluate intestinal motility of rats， transmission electron microscopy was used to observe colonic mucosa ultrastructure of rats， Western blot was used to determine the protein expression of AQP3， AQP8 in colon of rats， qPCR was used to determine the mRNA expression of AQP3， AQP8 in colon of rats. RESULTS The number of 24 h fecal grains， 24 h fecal water content and intestinal carbon propelling rate in EA Ⅱ group increased significantly(P<0.05)， protein and mRNA expression of AQP3， AQP8 in the model group was significantly higher than that of normal group in colon of rats， while significantly reduced after treatment with EA Ⅱ(P<0.05)， the ultrastructure of colonic mucosa were significantly improved after treatment with EA Ⅱ. CONCLUSION The mechanism of electroacupuncturestimulating Quchi(LI 11)， Shangjuxu(ST 37) on enhancing intestinal motility and improving the symptoms of constipation may be due to the reducing the expression of AQP3， AQP8 protein and AQP3， AQP8 mRNA in colonic mucosa of FC rats， thus reducing water reabsorption of colon， increasing fecal water content in colon， regulating water transport and improving the ultrastructure of colonic mucosa.

Effect and Mechanism of Leonurine on Rats with Ventricular Remodeling Induced by Isoproterenol

YUANLu, LIANGBo-zhi, LUOJian-hua, LULi-na, YANGHui, LILi, YANGDong-hua

2019, 35(2): 180-184.

[Abstract](755) [PDF 2037KB](544)

Abstract:
OBJECTIVE To observe the effect of leonurine on rats with ventricular remodeling induced by isoproterenol(ISO) and to explore the possible mechanism involved. METHODS SD rats (n=10) were used as normal control group， and 80 rats were given ISO by intraperitoneal injection daily for 2 weeks to establish the model of ventricular remodeling. The model rats were divided into 5 groups randomly as model group， low-dose leonurine (7.5 mg/(kg·d)) group， middle-dose leonurine (15 mg/(kg·d)) group， high-dose leonurine (30 mg/(kg·d)) group and p38MAPK inhibitor (0.3 mg/(kg·d)) group. After the treatment for 2 weeks， the pathological change of left ventricular myocardial tissues was observed by HE staining，and the expression of TGF-β1 was determined by the method of immunohistochemistry. The serum concentrations of ET-1 and NO were measured by ELISA and nitrate reductase methods， respectively. The expression of p38MAPK， MEF2，β-MHC and α-MHC mRNA was detected by qPCR， and the protein expression of ET-1， p-p38MAPK and MEF2 was determined by Western blot. RESULTS Compared with model group， the pathological change of ventricular remodeling in high-dose leonurine group was attenuated， and the serum concentrations of NO and the mRNA expression of α-MHC in left ventricular myocardial tissues of high-dose leonurine group were higher (P<0.05).The expression of TGF-β1， the serum concentrations of ET-1， the mRNA expression of p38MAPK，MEF2 and β-MHC mRNA， and the protein expression of ET-1， p-p38MAPK and MEF2 were lower than those in model group (P<0.05). CONCLUSION Leonurine attenuates ventricular remodeling in the rats induced by ISO， and it is potentially associated with inhibiting p38MAPK/MEF2 signaling pathway.

The Influence of Biyuan Mixture on p38MAPK Signaling Pathway and IL-9/IL-10 of Sinus Mucosa of ARS in Model Rats

NIPing-min, ZHAOJing-jing, LILe, XUEShan-shan, LIXiao-bei, MAHua-an, WUYong-jun

2019, 35(2): 185-188.

[Abstract](737) [PDF 1403KB](612)

Abstract:
OBJECTIVE To explore the clinical effect of Acute rhinosinusitis(ARS) treated by of Biyuan Mixture and its effects on p38MAPK signaling pathway and IL-9 and IL-10 of the sinus mucosa of ARS in modeled rats. METHODS To summarize the clinical curative effects of the modeled rats，20 cases of rats in each group，which was the Chinese medicine group，western medicine group and saline group. Those rats were respectively filled the stomach with Biyuan mixture， clarithromycin dispersibl tablets and limonene and pinene enteric soft capsules， saline. Meanwhile， 5 rats were selected as the blank group. The sinus mucosa IL-9， IL-10 and p-p38 were measured before treatment， and after treatment (0， 7， 14， 21 d). And the data were recorded for statistical analysis. RESULTS According to the symptom score comparison， both Chinese medicine and western medicine have the therapeutic effects. Compared with the blank group， the levels of IL-9 and p-p38 were significantly increased. The levels of IL-9 and p-p38 in the sinus mucosa of the Chinese medicine group and the western medicine group decreased significantly with the treatment cycle (P<0.05)， and there was no significant difference between the two groups. The level of IL-10 in the sinus mucosa of the Chinese medicine group， the western medicine group and the saline group gradually increased with the treatment cycle， and there were differences between the Chinese medicine group， the western medicine group and the saline group (P<0.05)， while there was no significant difference between the Chinese medicine group and the western medicine group. CONCLUSION IL-9 and IL-10 are involved in the immune process of the onset of ARS， and there is a certain relationship with p38MAPK pathway. ARS treated by Biyuan Mixture has a definite therapeutic effect and causes changes in the level of the above substances， indicating that this prescription can promote IL-10， inhibit p-p38 activation and may block p38MAPK pathway to inhibit IL-9.

Serum Metabolomic Difference of the Tongue Coatings in the Patients with Gastric Cancer by GC-MS

XUShuo, XIANGChun-jie, ZHUZhen-hua, WUJuan, GAOYa-nan, WANGRui-ping, ZHANZhen, ZHANGXiao-chun, ZHANGJun-feng

2019, 35(2): 194-198.

[Abstract](649) [PDF 1556KB](539)

Abstract:
OBJECTIVE To explore the serum metabolomic mechanism of the common tongue coatings (TCs) in the patients with gastric cancer. METHODS The gastric cancer patients (n=101) were divided into four groups: white thin TC group (n=42)， white thick TC group (n=9)， yellow thin TC group (n=28) and yellow thick TC group (n=22). Twenty-two healthy volunteers with white thin TC were recruited as controls. GC-MS was used to detect the serum metabolomic. Multi-factors analysis methods were conducted to screen the metabolic markers of the common TCs in gastric cancer patients， and enrichment analysis was carried out to predict the metabolic pathways. RESULTS Compared with the control group， the white thin TC of gastric cancer patients occupied 13 special metabolic molecules involved in 31 metabolic pathways， the white thick TC of gastric cancer patients held 15 special metabolic molecules involved in 26 metabolic pathways， the yellow thin TC of gastric cancer patients held 9 special metabolic molecules involved in 15 metabolic pathways， and the yellow thick TC of gastric cancer patients had 8 metabolic molecules involved in 12 metabolic pathways. The level of 4-aminobutyric acid elevated in all the gastric cancer patients were observed involving with tyrosine metabolic pathway. CONCLUSION Different TCs reflect characteristic serum metabolomic machanism in gastric cancer patients.

The Study of Compatibility Mechanism of Coptidis Rhizoma-Mume Fructus in Xiaoai Jiedu Prescription from Perspective of Chemical Components and Intestinal Microflora

SHIYue-hua, FANHui-sen, LIUXiao, XIAOPing, SHENWei-xing, CHENGHai-bo, SUNDong-dong

2019, 35(2): 199-204.

[Abstract](717) [PDF 1357KB](662)

Abstract:
OBJECTIVE To explore the difference of some alkaloids in Coptidis Rhizoma - Mume Fructus decoction and Xiaoai Jiedu Prescription， and compare their effects on the intestinal flora of mice. METHODS The separation was performed on Extend-C₁₈column (100 mm×2.1 mm， 1.8 μm) with 0.1% aqueous formic acid (A)-acetonitrile solution (B) as the mobile phase for gradient elution. The flow rate was 0.3 mL/min， column temperature was set at 35 ℃， and injection volume was 2 μL. Electrospray ionization source was applied for the analysis under positive ion model. The method was validated and the samples were determined under this condition. 50 mice were divided into ZC group， QFH group， QFL group， YDH group and YDL group according to different dosages. After 7 days of oral administration， fecal samples from each group were collected for 16S rRNA gene sequencing. RESULTS The established detection method showed good linearity within certain concentration ranges and the method validation results meet the relevant requirements. The contents of magnoflorine and columbamine in the single herb， herb pair and formula decoction were （4.433±0.133） and （8.905±0.154） mg/g，（3.545±0.033） and （9.170±0.051） mg/g，（5.287±0.038） and （13.861±0.690） mg/g， respectively. In addition， the abundance of Bacteroides in QF and YD were higher than that in the normal group， but the abundance of Firmicuteswas opposite. Interestingly， we found that the abundance of Verrucomicrobia and Proteobacteria in YDH were higher than other groups. CONCLUSION The method and related parameters were simple and reliable， which could be used to detect the content of related components. Compared with single herb， the content of columbamine was increased both in herb pair and Xiaoai Jiedu Prescription; the content of magnoflorine was decreased slightly in herb pair， while increased in Xiaoai Jiedu Prescription. After administering herb decoction， the change of intestinal flora diversity revealed Coptidis Rhizoma - Mume Fructus decoction and Xiaoai Jiedu Prescription could regulate the balance of intestinal system. The results further explain the compatibility mechanism of the two drugs in this prescription.

Identification of Chemical Constituents in Cuochuangxiao Lotion by UPLC-Q-TOF-MS

QIANYa-qin, ZHUYa-nan, LIUYi, JUJian-ming, DAIYing

2019, 35(2): 205-209.

[Abstract](602) [PDF 1512KB](502)

Abstract:
OBJECTIVE To analyze and identify the chemical constituents of Cuochuangxiao lotion. METHODS The sample was separated on an Acquity HSS T₃ reverse phase column (2.1 mm×100 mm， 1.8 μm) with the gradient elution consisted of 0.1% formic acid in MeCN and 0.1% formic acid in water as mobile phases at a flow rate of 0.3 mL/min. The electrospray ionization (ESI) source in positive or negative ion mode was used for MRM mode. The chemical constituents in Cuochuangxiao lotion were inferred and identified by accurate mass data， the element compositions analysis， the database of Scifinder， standards and relevant literatures. RESULTS 59 compounds from the Cuochuangxiao lotion were identified， mainly including anthraquinones， iridoids， organic acids and flavonoids. CONCLUSION The method is rapid and sensitive for the chemical constituent identification， which can provide the reference for clarifying the material basis and evaluation of the quality of Cuochuangxiao lotion.

Study on Fingerprint of Desmodium Caudatum Based on HPLC and Chemometrics

LIANGShuang, GANYang-ying, LUSen-hua, LIUJi-cheng, LIANGLu-sheng, JIANGYong-ning

2019, 35(2): 210-213.

[Abstract](654) [PDF 1302KB](611)

Abstract:
OBJECTIVE To establish a fingerprint of Desmodium caudatum based on HPLC and chemometrics， and to provide a scientific evaluation method for its quality control. METHODS A HPLC method was developed for the determination of 10 batches of Desmodium caudatum by using ZORBAX SB-C₁₈（5 μm，4.6 mm×250 mm） column with a detection wavelength of 330 nm and gradient elution with methanol-0.2% phosphoric acid solution at a flow rate of 1.0 mL/min. RESULTS The HPLC fingerprint of Desmodium caudatum was established， 13 common peaks were calibrated， and the similarity of fingerprint was above 0.9. Cluster analysis classified the samples into 4 categories， which was basically consistent with the results of principal component analysis. The effect of the quality difference of Desmodium caudatum was the result of the synergistic action of multi-components. CONCLUSION The fingerprint of Desmodium caudatum was analyzed by HPLC combined with chemometrics. The method has strong specificity， good precision， stability and reproducibility. It can be used as an effective method for quality evaluation and control of Desmodium caudatum.

Identification of Amomi Fructus and Its Adulterate Products by the Characteristic and Micro-morphology in Hebei Anguo Market

HOU Fang-jie, GUO Li-xiao, SONG Jun-na, ZHANG Dan, ZHENG Kai-yan, ZHENG Yu-guang, WANG Qian

2019, 35(2): 214-217.

[Abstract](585) [PDF 1130KB](1142)

Abstract:
OBJECTIVE Identifying the morphological properties and micro-characteristics of Amomi Fructus in order to distinguish the real from the false of Amomi Fructus， so as to provide some theoretical basis on Amomi Fructus identification. METHODS Through a stereomicroscope， observing the interior and exterior surface of the pericarp， seeds of Amomi Fructus，as well as the shape， the surface texture， the transverse section and the vertical section of the seeds. Using the technique of depth synthesis to get characteristic graphs of all the sections. Measuring the length and diameter of the fruit and the number of seeds particles in each chamber， analyzing the results on SPSS software. RESULTS Given that the TCM market is filled with amounts of fake Amomi Fructus， the domestic Amomi Fructus were mixed with muricarpume Elm， while most of the imported with thyrsoideum Gagnep. Acording to the differences in morphological properties ， and the statistical measurements of length of fruits ，there is a method which can be used to differentiate. CONCLUSION The method of analyzing the morphological properties and micro-characteristics of Amomi Fructus can be applied to identify Amomi Fructus. It is more accurate and faster.

Professor Zhao Yuyong's Experience in Treating Renal Hematuria

YANGHong-juan, CHENJing-wei, PANLi, WEIHua-juan, CAIJi-min

2019, 35(2): 218-220.

[Abstract](672) [PDF 1062KB](480)

Abstract:
Professor Zhao Yuyong thinks the root of renal hematuria is deficiency of lung， spleen and kidney. The occurrence of renal hematuria is spleen deficiency failing to astringe. While the branch is heat and stasis. Stasis and obstruction in the kidney collateral is the basic pathogenesis. The treatment principle is to reinforce healthy qi and treat the root， focusing on nourishing lung， spleen and kidney and dispelling pathogen to treat the branch， cooling blood and activating blood to stop bleeding. Moreover， for stasis and obstruction in the kidney collateral， insect drugs should be applied to dredge collaterals. In the process of renal hematuria treatment， the thought of prevention in TCM is also embodied.

Professor Shi Zaixiang's Experience in Treating Angina Pectoris with Qi Sinking and Blood Stasis Syndrome After Percutaneous Coronary Intervention

ZHANGZhan-lin, YINXin, GUOLi-heng

2019, 35(2): 221-222.

[Abstract](639) [PDF 1046KB](516)

Abstract:
This paper introduces Professor Shi Zaixiang's clinical experience in treating angina pectoris after percutaneous coronary intervention(PCI) with qi sinking and blood stasis syndrome. It is believed that the pathogenesis of angina pectoris after PCI with qi sinking and blood stasis syndrome is pectoral qi sinking and blocking collaterals. The key to syndrome differentiation lies in pectoral qi sinking. It is suggested that the treatment should be based on raising the sunken and removing blood stasis. However， it should not only supplement qi， but also be used together with raising drugs， so that the pectoral qi can rise and fill the chest， and the qi can flow through the blood smoothly without pain.

Evaluation of the Safety of Contraindicational Chinese Medicine in Pregnancy Based on the Strategy of Amniotic Fluid Metabolomics

XUJian-ya, LISi-ying, XIETong, SHENCun-si, JIJian-jian, ZHOUHui-fang, SHANJin-jun

2019, 35(2): 223-228.

[Abstract](728) [PDF 1150KB](722)

Abstract:
Medication in pregnancy is inevitable， and the public urgently needs accurate toxicological data to evaluate the safety of traditional Chinese medicine. Amniotic fluid can reflect the health status of the fetus as well as the mother at the same time. The change of amniotic fluid metabolism pattern is related to the pathogenesis during pregnancy. In this paper， the related researches on amniotic fluid metabolomics in fetal prediction were summarized and we took an our previous study as an example to discuss the advantages and feasibility of amniotic fluid metabolomics in the safety evaluation of Chinese medicine during pregnancy. We believe that the metabolomics of amniotic fluid， as an innovative tool with higher sensitivity and relevance， will have broad application prospects in toxicity screening and mechanism research of Chinese medicine.

Effects and Mechanisms of Traditional Chinese Medicine on Drug-resistant Bacteria: Research Progress and Future Directions

CHENG Cheng, ZHANG Wei, ZHU Bo, SHI Li-yun

2019, 35(2): 229-233.

[Abstract](1166) [PDF 1117KB](1014)

Abstract:
Since the discovery of penicillin， antimicrobial drugs have been widely used in the treatment of infectious diseases， which contributes substantially to the improvement of human health and the progression of medical science. However， with the wide and long-term use of antibiotics， various multiple-drug-resistant (MDR) bacteria have been increasingly emerging， which become the serious precludes for conquering infectious diseases. Traditional Chinese medicine generally constitute of multiple components， targets and functions with no obvious drug-resistance during long-term practicing. Hence， exploring new antibiotics from Chinese herbal medicine has become the rational choice for the resolution of bacterial resistance. In this review， the research progress on the traditional Chinese medicine with anti-bacterial property and on the mechanisms involved is summarized.

Research Progress on the Effect of Intestinal Flora on Depression Based on "Brain-Gut" Axis

TAOWei-wei, DONGYu, LIULi, XIAODong, WUHao-ran, WUHao-xing, CHENGang, DILiu-qing, WANGHan-qing

2019, 35(2): 234-240.

[Abstract](907) [PDF 1299KB](1591)

Abstract:
In recent years， with the deepening of the research on the regulation of intestinal flora， the "brain-gut" axis， which is used to describe the complex network relationship between gastrointestinal microflora and their hosts. It is a bidirectional information regulation pathway based on the presence of intestinal flora in mammals and plays an important role in human health and disease process. Depression is a long and recurring chronic mental illness that differs from simple mood disorders. Studies have shown that intestinal flora can significantly influence host stress response， anxiety， depression and cognitive function through the brain-gut axis. Based on the intestinal flora， this paper reviewed the research status and mechanism of depression， aiming to provide basis and reference for the treatment of depression.

2019 Vol. 35, No. 2

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