2019 Vol. 35, No. 1

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Thinking on the Application of Blood-Activating and Stasis-Resolving Medicine in Anti-Tumor and Metastasis
TANG De-cai
2019, 35(1): 1-4.
[Abstract](2114) [PDF 1053KB](1394)
Abstract:
It is believed that blood stasis exists in the whole process of tumor， and blood stasis coexisting with deficiency is the basic pathogenesis of tumor occurrence and development. The static adhesion formed by tumor thrombus adhering to the inner wall of blood vessel， namely blood stasis， is the core link of tumor metastasis. Then， it is proposed that static adhesion is the origin of dynamic metastasis and dynamic metastasis is the development of static adhesion， while healthy qi deficiency and blood stasis is the basic pathogenesis of tumor metastasis. Therefore， blood-activating and stasis-resolving medicine combined with invigorating-qi medicine can be used to cut off the trend of tumor metastasis， that is， breaking the static adhesion is the key of Chinese medicine intervention in tumor metastasis prevention. Therefore， choosing the right variety， grasping the time of use， and dialectically combining other medicine are the important links to ensure the curative effect.
Correlation Between Spleen-Qi Deficiency Pathogenesis and Autophagy Dysfunction Based on Spleen and Stomach Theory
YANGLiang-jun, LIUWei, HUZhi-peng, ZHENGJia-yi, LISi-yi, PANHua-feng
2019, 35(1): 5-8.
[Abstract](780) [PDF 1051KB](969)
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Cell autophagy， as a process of cell decomposition and metabolism， has a certain correlation with the physiological functions of spleen and stomach in traditional Chinese medicine (TCM) in maintaining the body's growth， development， immune defense and other functions. Based on the spleen and stomach theory， starting from the spleen-qi deficiency pathogenesis such as the body's transport disorder， insecurity of defense qi and the disorder of qi and blood supply and distribution， and combining with the research results of modern medicine on the decline of autophagy in cell metabolism， immune defense and aging， this paper analyzes the relationship between the spleen-qi deficiency pathogenesis and autophagy dysfunction， and puts forward that the decline of autophagy can be regarded as the microscopic pathogenesis of spleen-qi deficiency in TCM， and provides ideas for revealing the microscopic nature of spleen-qi deficiency.
Syndrome Differentiation of Pulmonary Fibrosis from Yin-Fire Theory
YANG Lan, ZHANG Ling-shan, FAN Xin-sheng
2019, 35(1): 9-11.
[Abstract](838) [PDF 1035KB](803)
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This paper introduces the understanding and pathogenic characteristics of yin-fire in the past dynasties， probes into the pathogenesis and syndrome differentiation of pulmonary fibrosis form the yin-fire theory， and analyzes the therapeutic principles and prescription for treating yin-fire in lung.
Analysis of Meridian Syndrome Differentiation under Two Meridian Theory Models
LIU Yun, CHEN Cai-yi
2019, 35(1): 12-15.
[Abstract](936) [PDF 1049KB](936)
Abstract:
This paper introduces the basic contents of meridian syndrome differentiation under the initial centripetal mode of meridian theory and the later cyclical mode of meridian theory. Compared with the Zangfu-meridian cyclical model， the initial heart-oriented meridians model focuses more on the change of meridians and qi in the limbs. The differentiation of meridians in this model is intended to illustrate the relationship between the distant parts of the body and reflects the core and characteristics of acupuncture and moxibustion diagnosis and treatment. The Zangfu-meridian cyclical model will belong to Zangfu， so that the differentiation of meridians under the guidance of this pattern will focus more on the exploration and summary of the overall function of the body， aiming to explain the pathological and physiological relationship between the diseases and Zangfu and Meridian， so as to clarify the etiology and pathogenesis. It is believed that only by clearly distinguishing the characteristics and differences between the two meridians can acupuncture and moxibustion be better performed in clinical practice.
Clinical Study of Liuwei Buqi Granules Combined with Seretide in the Treatment of Patients with COPD at Stable Stage
ZENGShi-jie, WANGDan-yang, ZHANGZhi-qiang, HU-Jian, LIZe-geng
2019, 35(1): 16-20.
[Abstract](772) [PDF 1077KB](553)
Abstract:
OBJECTIVE To observe the clinical effect of Liuwe Buqi granule combined with Seretide on patients with chronic obstructive pulmonary disease (COPD) at stable stage (deficiency of lung and kidney qi). METHODS 60 patients with COPD at stable stage were randomly divided into control group (30 cases) and observation group (30 cases). The control group was treated with Seretide inhalation on the basis of routine western medicine treatment， and the observation group was treated with Liuwei Buqi granule on the basis of the control group for 8 weeks. The clinical efficacy and the change of acute exacerbation times， CAT score and serum amyloid A (SAA) level of the two groups were compared before and after treatment. RESULTS The total effective rates of observation group and control group were 93.33% and 70.00%( P <0.01)， respectively. After treatment， the times of acute exacerbation in observation group was significantly lower than that in control group ( P <0.01). The CAT score and the serum SAA level in the two groups were significantly decreased before and after treatment ( P <0.01). After treatment， the improvement of expectoration， fatigue and self-sweating in observation group was better than that in control group( P <0.05， P <0.01). The CAT score in observation group was significantly different from that in control group ( P <0.01). The serum SAA level in observation group was significantly lower than that in control group ( P <0.01). CONCLUSION Liuwei Buqi granules combined with Seretide can effectively improve the clinical symptoms of patients with COPD at stable stage (deficiency of lung and kidney qi)， reduce the times of acute exacerbation and reduce the level of serum SAA. The clinical curative effect is satisfactory.
Clinical Observation of Plasma Exchange Combined with Liangxue Huayu Formula on Chronic Severe Hepatitis
XU Xiao-li, PEI Jian-hong, YI Ting, LI Hong, ZHENG Ting-ting
2019, 35(1): 21-24.
[Abstract](634) [PDF 1049KB](527)
Abstract:
OBJECTIVE To observe the clinical efficacy of plasma exchange combined with Liangxue Huayu Formula on treating severe hepatitis. METHODS Patients diagnosed with severe hepatitis in observation group (43 cases) were treated with plasma exchange and Liangxue Huayu Formula. While the control group (40 cases) were only received plasma exchange and basic treatment. The liver function index， coagulation index and the change of T cell subsets the two groups were recorded after eight weeks of treatment. RESULTS The effective rate of the observation group was 81.39% while that of the control group was 65%. There was a statistical difference between the two groups ( P <0.05). The clinical symptoms， TCM syndrome score and liver function index， coagulation index， and changes of T cell subsets were significantly improved after treatment( P <0.05). The improvement of TCM syndrome score， indicators of liver function and coagulation， the percentage of CD4+ and CD4+/CD8+ in observation group were significantly better than that of the control group( P <0.05， P <0.01). CONCLUSION Plasma exchange combined with Liangxue Huayu Formula can effectively treat severe hepatitis， as well as improving patients' liver function， coagulative function and T cell subsets.
Effect of Tongxie Yao Fang Combined with Sijunzi Decoction on Brain-Gut Peptide in Irritable Bowel Syndrome Patients with Diarrhea Type
ZHUOBing-fan, ZHANGYan-qing, NINGXiao-yan
2019, 35(1): 25-28.
[Abstract](930) [PDF 1062KB](650)
Abstract:
OBJECTIVE To explore the clinical efficacy and the brain-gut peptide effect of Tongxie Yao Fang combined with Sijunzi Decoction in the treatment of Irritable Bowel Syndrome (IBS-D) patients with diarrhea type. METHODS 53 patients diagnosed with IBS-D according to Rome Ⅲ standards were randomly divided into two groups. 27 patients in treatment group were treated with Tongxie Yao Fang combined with Sijunzi Decoction， and 26 patients in control group were given by the Pinaverium Bromide and Bifidobacterium Tablets for four weeks. The changes of clinical symptom index， quality of life (IBS-QOL)， neuropeptide Y (NPY)， calcitonin gene related peptide (CGRP) and vasoactive intestinal peptide (VIP) before and after treatment were observed. RESULTS The total effective rates in treatment group were 92.6%， significantly higher than 76.9% in control group ( P <0.05). The clinical symptom index in treatment group was significantly lower than that of the control group after treatment( P <0.05). And the score of IBS-QOL in treatment group was higher than that in control group ( P <0.05). The level of plasma NPY in both groups increased significantly after treatment， while CGRP and VIP levels decreased significantly ( P <0.05， P <0.01)， and the difference between the two groups was statistical significance ( P <0.05). CONCLUSION Tongxie Yao Fang combined with Sijunzi Decoction can significantly improve clinical symptom and QOL of IVS-D patients， and its mechanism may through the increase of plasma NPY level and the decrease of CGRP and VIP level， regulating the balance of brain-gut peptide secretion.
Effect of Baitouweng Decoction Enema in the Treatment of Ulcerative Colitis Patients and Its Influence on T cell Subsets
XUJia-ping, MAChao-qun
2019, 35(1): 29-31.
[Abstract](749) [PDF 1041KB](568)
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OBJECTIVE To investigate the efficacy of Baitouweng decoction enema in the treatment of ulcerative colitis patients and its effect on T cell subsets. METHODS 124 patients with ulcerative colitis admitted to our hospital from January 2014 to March 2018 were randomly divided into control group and observation group. Both groups were given mesalazine and the observation group was additionally treated with Baitouweng decoction enema. The effectiveness rate of treatment， colonscopy score， length of stay， UC activity， T cell subsets change and the incidence of adverse reactions were compared between the two groups. RESULTS After 30 days of treatment， the effectiveness rate of treatment in observation group(93.5%) was significantly higher than that in control group (72.6%)， P <0.05. The length of stay in observation group was significantly lower than that in control group ( P <0.05). The DAI score in observation group was significantly lower than that in control group， P <0.05; The CD3+ and CD8+ in peripheral blood of both groups showed a downward trend while both CD4+ and CD4+/CD8+ showed an upward trend， and the change range of the observation group was significantly larger than that of the control group， P <0.05. The incidence of adverse reactions in observation group was 9.7% and the control group counterpart was 8.1%. The difference was not significant( P >0.05). CONCLUSION Baitouweng decoction enema can effectively improve the efficacy of UC， reduce the UC activity index and the length of stay， improve the immune function of patients， which is worthy of further promotion and application in clinical practice.
2019, 35(1): 32-35.
[Abstract](644) [PDF 1055KB](579)
Abstract:
Clinical Observation of Chaishao Sijin Decoction on Preventing Recurrence of Choledocholithiasis after ERCP
TAOMing-hao, LUXi-rong, DAIYan-miao, XUEBo-yu
2019, 35(1): 36-38.
[Abstract](776) [PDF 1042KB](772)
Abstract:
OBJECTIVE To study the clinical effect of Chaishao Sijin Decoction in preventing recurrence of choledocholithiasis after ERCP. METHODS 120 cases of ERCP for choledocholithiasis from January 2014 to December 2016 in gastroenterology and hepatology department of Kunshan Hospital of Traditional Chinese Medicine were selected. They were randomly divided into treatment group and control group according to the random number table， 60 cases in each group. The treatment group was treated with 400 ml Chaishao Sijin Decoction orally one dose a day， take it warm and twice in the morning and evening. Add or subtract herbs according to the syndrome. The control group was given by 250 mg ursodeoxycholic acid orally three times a day. Two groups were treated continuously for 6 months. The indexes of the serum total bilirubin (Tbil)， direct bilirubin (Dbil)， alkaline phosphatase (ALP)， glutamyl transferase (GGT)， clinical symptoms (including abdominal pain， abdominal distention， nausea， poor appetite) were detected in two weeks after the surgery. Recurrence of choledocholithiasis were recorded 6， 12 and 18 months after operation. RESULTS The recurrence rate of choledocholithiasis in treatment group 6， 12 and 18 months after operation was slightly lower than that in control group but the difference was not statistically significant ( P >0.05); The treatment group was superior to the control group in improving symptoms of abdominal pain， abdominal distension， nausea and poor appetite ( P <0.05); The treatment group was superior to the control group in improving serum Tbil， Dbil， ALP and GGT( P <0.01). CONCLUSION Chaishao Sijin Decoction can effectively prevent recurrence of choledocholithiasis after ERCP and improve clinical symptoms and serum biochemical indexes caused by choledocholithiasis.
Using Metabolomics to Explore the Essence of Damp Heat Accumulation Syndrome of Infantile Human Cytomegalovirus Hepatitis Based on UHPLC-MS System
LIWei-wei, WANGShou-chuan, SHANJin-jun, XIETong, LINLi-li, HELi-li, DULi-na, YANGYan
2019, 35(1): 39-46.
[Abstract](670) [PDF 1574KB](689)
Abstract:
OBJECTIVE Using metabolomics to explore the essence of damp heat accumulation syndrome of infantile human cytomegalovirus hepatitis. METHODS 20 infantile HCMV hepatitis patients with damp heat accumulation syndrome and 20 healthy infants were recruited for investigation. Plasma and urine samples were analyzed using ultra-high-performance liquid chromatography-linear trap quadruple/orbitrap mass spectrometry (UHPLC-LTQ/Orbitrap-MS) to determine the alterations in metabolomic profiles of this syndrome. Orthogonal partial least squares-discriminate analysis (OPLS-DA) was applied to analyze the UHPLC-MS data obtained from these samples. The specificity and sensitivity of potential biomarkers were assessed according to the area under the receiver operator characteristic (ROC) curves. RESULTS The metabolomic analysis yielded 1076 plasma compounds and 414 urine compounds. Among them， 22 plasma and 7 urine differential metabolites relating to sphingolipid， glycerophospholipid， histidine， glycerolipid， and fatty acid metabolism were identified. Sphingomyelin and triglycerides were screened as potential biomarkers and showed excellent discriminant performance. CONCLUSION This research can provide insights into the essence of HCMV hepatitis damp heat accumulation syndrome.
Effect of Modifie Drehmanniae Prescription on TGF-β1-CTGF Signaling Pathway to Improve Renal Interstitial Fibrosis
YANGZhi-chao, YANGJian-jun, MAJun, HUXiang-nong, LIUYuan, ZHANGLi
2019, 35(1): 47-52.
[Abstract](728) [PDF 3259KB](518)
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OBJECTIVE To study the effect of modified Rehmannia Recipe on renal interstitial fibrosis in rats with partial ureteral obstruction (PUUO) and to explore its possible mechanism. METHODS Eighty SD rats were randomly divided into control group， model group， low dose group and high dose group ( n =20). In the model group， the model of PUUO was established by lumbar large muscle tube embedding method. Only the left kidney was removed in the control group.High dose group (2 mL/d) and low dose group (1 mL/d) were treated with Modified rehmannia decoction， the control group and the model group were given the same amount of normal saline. The levels of serum creatinine (SCr)， urea nitrogen (BUN) and 24 h urinary protein were determined in each group. Blood samples were collected from each group at 4th week and 8th week after modeling. Measurement of renal tissue size， renal cortical thickness. The renal histopathological changes were observed by staining with HE and Masson， and the renal fibrosis was observed. The expressions of transforming growth factor-β1 (TGF-β1)， connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in renal tissue were detected by immunohistochemistry. The expression of TGF-β1 and CTGF mRNA was detected by qPCR. RESULTS At 4th week， inflammatory cell infiltration， local focal necrosis and necrosis were observed in the model group， and more collagen fibrosis was formed. Compared with the model group， the pathological changes of the high and low dose group were not obvious， and only a small interstitial fibrosis was widened. After 8 weeks: model group lymphocyte infiltration， glomerular necrosis， the structure completely changed. Interstitial fibrous hyperplasia. In the high and low dose group， the infiltration of inflammatory cells was lighter than that of the model group， the staining of the collagen was lighter and the high dose group had the smallest change. The average positive expression rate of TGF-β1， CTGF and α-SMA protein was not significantly different between the two groups at 4th week compared with the model group. At the 8th week， the two drug treatment groups had Significant difference ( P <0.01). Comparison of TGF-β1， CTGF mRNA expression was found when the low-dose group 4 weeks with model group had no differences as compared to high dose group and the model group were different ( P <0.05) as compared to 8th week. CONCLUSION Modified rhubarb may reduce the expression of TGF-β1-CTGF and down-regulate the expression of α-SMA， reduce the pathological changes of renal fibrosis and delay the development of renal fibrosis.
The Inhibitory Effect of Astragaloside Ⅳ on Epithelial Mesenchymal Transition of Human Peritoneal Mesothelial Cells by Regulating Akt Signaling
YU Man-shu, SHI Jun, ZHAO Jun-yi, ZHU Yi-lin, SHENG Mei-xiao
2019, 35(1): 53-57.
[Abstract](774) [PDF 1558KB](766)
Abstract:
OBJECTIVE To observe the effect of Astragaloside Ⅳ (AS-Ⅳ) on EMT of human peritoneal mesothelial cells (HPMCs) HMrSV5， exploring the function of Akt signaling and regulatory mechanism of AS-Ⅳ. METHODS We used TGF-β1 to establish EMT model of HMrSV5. EMT marker proteins， β-catenin and Akt signaling-related proteins were detected by Western blotting analysis. β-catenin and EMT-related mRNA were expressed by Real-time PCR analysis. Compared with MK2206， Rapamycin and Insulin， the effect of AS-Ⅳ on EMT and β-catenin was observed by Western blotting. RESULTS ①The EMT model of HMrSV5 was established by TGF-β1. TGF-β1 increased β-catenin expression and activated Akt signaling. ②AS-Ⅳ could alleviate TGF-β1-treated EMT of HMrSV5， decrease β-catenin expression， as well as inhibit activation of Akt signaling. ③EMT of HMrSV5 and β-catenin were regulated by Akt signaling， MK2206 and Rapamycin had the similar effect with AS-Ⅳ. ④Insulin could reduce obviously inhibitory effect on EMT and β-catenin of AS-Ⅳ. CONCLUSION TGF-β1 can induce EMT of HMrSV5 and upregulate β-catenin， AS-Ⅳ has inhibitory effect on EMT and β-catenin of HMrSV5 by regulating Akt signaling.
Study of Gua Sha's Effect on the Immune Functions of the Skin and the Body in Healthy Individuals
WUXian-xian, ZHANGJin, DINGZhi
2019, 35(1): 58-62.
[Abstract](730) [PDF 1787KB](686)
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OBJECTIVE In this study， we investigate Gua Sha's therapeutic mechanism from immunological approaches. METHODS Gua Sha was performed on the back of Balb/c mice and the effects on the morphology of the skin tissue were observed using histological staining. Flow cytometry and ELISA were employed to determine the proportions of local immune active cells and local and systemic cytokine levels， respectively. Antigen-specific subtype antibody titers against intradermal and intranasal vaccines were utilized to assess Gua Sha treatment on the immunological functions of the skin locally and systematically. RESULTS Blood vessel expansion， erythrocyte extravasation and increased ratios of immune active cells were observed in the skin tissue following treatment， indicating an enhanced microcirculation. Pro-inflammatory cytokines were found up-regulated， and immunosuppressive cytokines downregulated， in both treated and untreated skin areas. These showed that Gua Sha can cause local and systemic effects. Intradermal delivery of a model antigen following Gua Sha treatment induced about three-fold higher IgG titers with a more Th1-biased antibody subtype profile. CONCLUSION Gua Sha treatment can boost skin microcirculation， enhance and regulate the innate and adaptive immune functions of the skin， which contributes partially to its therapeutic effects.
Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells
LIUMin, LIUChun-liang, LIANGGuo-qiang, YAOFei, ZHANGXiao-die, CHENTing, YUANYa-qin, WUShi-liang
2019, 35(1): 63-67.
[Abstract](645) [PDF 2163KB](509)
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OBJECTIVE Based on glycosyltransferase β3GnT8， to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang. METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay. By interference and transfection of β3GnT8， high and low expressed β3GnT8 MDA-MB-231/TAX cell lines were obtained. Western blot was used to detect the protein expression of β3GnT8， and drug resistance protein BCRP and P-gp. Lectin flow cytometry was used to detect β3GnT8 fluorescence intensity on β3GnT8 transfected MDA-MB-231/TAX cells. RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells， and the inhibitory rate was 50.16% after 72 h. Zhuidu Fang significantly downregulated the protein expression of β3GnT8， BCRP and P-gp. The protein expression of β3GnT8 showed a positive correlation with BCRP and P-gp. CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines， which is associated with the downregulation of the expression of β3GnT8， BCRP and P-gp.
Effects of Guizhi Fuling Capsule on Apoptosis of Heterotopic Endometrial Cells， VEGF and Estrogen and Progesterone in the Serum of Rat EM Model
XIAOXin-chun, LIHai-yan, LIXin-qin
2019, 35(1): 68-72.
[Abstract](607) [PDF 2031KB](650)
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OBJECTIVE To observe the effect of VEGF， estrogen and progesterone in serum on ectopic endometrial cell apoptosis in EMS rats and explore the mechanism of Guizhi Fuling Capsule. METHODS Establish rat endometriosis model by autologous transplantation methods， Four weeks after surgery， The rats were randomly divided into model group， mifepristone group， Sanjie analgesic capsule group， Guizhi Fuling pill high， medium and low dose group，with 15 rats per group. Four weeks after the drug intervention， the ectopic endometrial cell apoptosis was detected by TUNEL method The contents of estrogen and progesterone in serum were measured，The expression of caspase-7， VEGF and survivin was detected by immunohistochemistry. RESULTS The apoptotic rate of ectopic endometrial cells in Guizhi Fuling high dose group was significantly higher than model group，The expression of caspase-7 in ectopic endometrium of Guizhi Fuling high dose group and mifepristone group was significantly higher than model group，VEGF， survivin expression and the content of estrogen and progesterone in serum were significantly decreased. CONCLUSION The mechanism of the treatment of EMT by Guizhi Fuling Capsule， may be closely related to induced ectopic endometrial cell apoptosis and decreased endometrial VEGF expression and down-regulates the high expression of survivin and regulated levels of estrogen and progesterone in serum.
Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats
WANGXu, SHAOXin, XUHai-yan, YELi-fang, XUXi-ru, YANGXiao-qing
2019, 35(1): 73-77.
[Abstract](716) [PDF 1237KB](798)
Abstract:
OBJECTIVE To observe the effect of Tangzhiqing on the endoplasmic reticulum stress related factors GPR78， CHOP and Caspase-12 in hippocampus of diabetic rats， and to explore its mechanism of improving cognitive function in diabetic rats. METHODS The model of type 2 diabetes mellitus rats was established by intraperitoneal injection of low-dose of streptozotocin combined with high-sugar and high-fat diet， After 8 weeks of intervention with high， medium and low-dose of Tangzhiqing， serum fasting glucose (FPG) and fasting insulin (FINS) were detected by ELISA， the index of insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated; the expression levels of endoplasmic reticulum stress related genes GPR78， CHOP and Caspase-12 mRNA and protein in hippocampus were detected by qPCR and Western blot assay. RESULTS Different dosages of Tangzhiqing significantly decreased FPG and HOMA-IR ( P <0.01); except the low dose group， Tangzhiqing significantly improved ISI ( P <0.01); no significant difference existed in FPG level among the 3 groups ( P >0.05). Compared to the model group， the relative expression of GRP78 mRNA and protein in the 3 groups of Tangzhiqing increased significantly， and the expression of CHOP and Caspase-12 mRNA in these 3 groups decreased ( P <0.01). CHOP and Caspase-12 in middle and high dose group decreased significantly ( P <0.01). CONCLUSION Tangzhiqing can control blood sugar， improve insulin resistance， increase insulin sensitivity and regulate the expression of GRP78， CHOP， Caspase-12 in hippocampus.
Lipomics Study of Jinxin Oral Liquid for RSV Infection in Mice Based on UHPLC-Q-Exactive Orbitrap/MS
JIJian-jian, YANGRui, XIETong, SHENCun-si, LINLi-li, WANGShou-chuan, SHANJin-jun
2019, 35(1): 78-84.
[Abstract](776) [PDF 1589KB](613)
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OBJECTIVE Alipidomic method was applied to determine the lipidome in serum of mice based on high-resolution mass spectrometry (Q-Exactive Orbitrap). We investigated the changes of lipids in serum of mice with RSV to explore the regulation of Jinxin oral liquid on lipid metabolism caused by RSV. METHODS The serum samples from the blank group， the RSV model group and Jinxin oral liquid group mice were collected， and the lipids of these samples were extracted and further analyzed by high-resolution mass spectrometry. Multivariate statistical methods such as principal component analysis (PCA) and cluster analysis (CA) were used to analyze the lipid profiles， and the Kruskal-Wallis test and fold change were performed to find the differential lipids. RESULTS The lipids of serum between the blank control group and the RSV model group could be well distinguished， RSV infection in mice with metabolic abnormalities of lipids， the specific performance of the PC， PE was significantly upregulated， Jinxin oral liquid have a certain role in these lipids CONCLUSION RSV infection caused disturbances of lipid in vivo， and Jinxin oral liquid has a certain regulatory effect on the lipid metabolism disorder caused by RSV model.
Simultaneous Determination of 16 Main Chemical Components in Aqueous Extract of Sinisan Based on UHPLC-QqQ-MS Technology
ZHOUJia, CAIHao, DUANYu, PEIKe, XUYang-yang, LIUJing, NIUMin-jie, ZHANGYa-ting
2019, 35(1): 85-89.
[Abstract](769) [PDF 1330KB](666)
Abstract:
OBJECTIVE A method for simultaneous determination of 16 chemical components in aqueous extract of Sinisan was established. METHODS UHPLC-QqQ-MS technology was employed. Scan was conducted in negative ion mode and quantitative determination was carried out by MRM paired ions. RESULTS Saikosaponina A， saikosaponina B2， saikosaponina C， saikosaponina D， hesperitin， cianidanol， liquiritin， glycyrrhizic acid， liquiritigenin， chlorogenic acid， gallic acid， paeoniflorin， albiflorin， isoliquiritigenin， isochlorogenic acid A， and hesperidin all have good responses in negative ion mode. CONCLUSION The established method is specific， rapid， sensitive， accurate， and reliable， which can simultaneously determine contents and accurately give mass information of compound to achieve the effects of component identification， and also it can provide references for quality control and evaluation of aqueous extract of Sinisan.
Qualitative and Quantitative Study of Main Efficacy Components Between Rhubarb Pairing with Eupolyphaga and Dahuang Zhechong Pills
ZHANGXi-qiong, WULi, CHENCai, LIWei-dong
2019, 35(1): 90-94.
[Abstract](821) [PDF 1399KB](742)
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OBJECTIVE To comparative analysis of the changes of pharmacodynamics between Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga by means of high performance liquid chromatography (HPLC)， using the research ideas of traditional Chinese medicine compound prescriptions. METHODS The chromatographic column was Thermo Hypersil Gold， mobile phase 0.1% formic acid water - methanol， gradient elution， column temperature: 30 ℃， detection wavelength 270 nm， to establish the fingerprints of Dahuang Zhechong Pills and main medicine paris， and to quantitatively analyze the content of anthraquinones in rhubarb as well as hypoxanthine. RESULTS Establish liquid fingerprint respectively in Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga. A total of 32 common peaks were identified and 27 drugs were identified. In the quantitative study， we found that the content of five kinds of rhubarb anthraquinone in the drug was higher than that of the whole body， including 1.6 times of aloe-emodin， 1.48 times of rhein， 2.23 times of emodin， 2.43 times of chrysophanol， 2.48 times of physcion， hypoxanthine content was more than the whole group. CONCLUSION Pairs and whole group of Dahuang Zhechong Pills both have a certain anti-liver cancer effect. Although drugs on the component anthraquinones are higher than the whole side， the role of anti-liver cancer to the whole for the excellent， suggesting that the whole low-dose multi-component， multi-target role in the importance. In this study， we provide reference for the determination of anthraquinone and hypoxanthine in Dahuangzhechong pills and provide the idea for further exploring the material basis of liver cancer.
Research on Full Wavelength Fusion Fingerprint of Brown Sugar for Pharmaceutical Excipient
LUYang, SHENZi-bo, LIUJie, DONGWei, LICun-Yu, ZHENGYun-feng, PENGGuo-ping
2019, 35(1): 95-98.
[Abstract](804) [PDF 1548KB](688)
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OBJECTIVE To research and establish the full wavelength fusion fingerprint of the brown sugar for pharmaceutical excipient. METHODS With a Durashell C18-AM column(4.6 mm×250 mm，5 μm，100 A)，gradient elution was performed by the mobile phase acetonitrile(A)-0.1% trifluoroacetic acid solution(B) at the flow rate of 1.0 mL/min. The column temperature was kept at 25 ℃.The detecting condition was performed with the photodiode array detector at the full wavelength from 210 nm to 400 nm. The data was fused by Waters Empower Chromatography Data Software and analysed with the similarity evaluation system for chromatographic fingerprint of chinese medicine. RESULTS There are 10 common peaks which were founded from fingerprints by similarity analysis， in the 35 batches of brown sugar for pharmaceutical excipient.The fingerprints provided credible similarities of brown sugars for pharmaceutical excipient which were greater than 0.9. CONCLUSION This method which is stable and accurate with good repeatability provides the basis for controlling the fingerprint of brown sugar for pharmaceutical excipient.
Studies on the Dynamic Accumulation Analysis of Metabolites of Pseudostellariae Radix in Jiangsu Province
ZOU Li-si, HOU Ya, MA Yang, LIU Xun-hong, LUO Yi-yuan
2019, 35(1): 99-103.
[Abstract](677) [PDF 1715KB](739)
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OBJECTIVE To analyze the change of chemical components of Pseudostellariae radix from different harvest period in Jiangsu. METHODS UPLC-Triple TOF-MS/MS and tandem mass spectrometry combing with Mass data， databases and related software for total ion current literature major molecular ion peaks attributable was used to determine 16 batches of samples. SIMCA-P software was used to analyze the principal components. RESULTS 12 compounds were identified， and the figure below showed that the PCA， samples of June and beginning of July were in close proximity on the plot， indicating that the metabolites of these two periods were similar; samples from end of July and August were far away， showing big chemical differences. 10 kinds of markers were picked from loading chart by significant differences. These markers of Pseudostellariae Radix collected from different harvest time showed different changing laws. CONCLUSION To provide the basic data for revealing the different harvest time of Pseudostellariae Radix in Jiangsu metabolite accumulation pattern.
Professor Jin Shi's Clinical Experience in Treating Systemic Lupus Erythematosus with Central Nervous Damage with Traditional Chinese Medicine
MANuo-sha, WANGYue
2019, 35(1): 104-105.
[Abstract](763) [PDF 1021KB](797)
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Professor Jin Shi believes that wind， phlegm， blood stasis and toxin are the main pathological factors of epileptic seizures associated with systemic lupus erythematosus with central nerve damage， and it is commonly used to add and subtract Dingxian decoction. Patients with central nervous system damage characterized by paroxysmal headache should cooperate with the prescription of Huoxue Dingtong decoction. If the symptoms of central nervous system damage are not serious， the specimen should be treated together and treated with Dingxian decoction and Langchuang formula， which benefits kidney and resolve toxin.
Clearing Pestilence and Resolving Toxins Formula in Treating Influenza on Children
ANLi, LIUYu-ling, ZHANGYa-ting, WANGShou-chuan
2019, 35(1): 106-108.
[Abstract](694) [PDF 1037KB](531)
Abstract:
Professor Wang Shouchuan believed that the pestilence theory of epidemic febrile diseases could be applied as the guideline to understand the etiology and pathogenesis of children's influenza. He proposed that children's influenza should be divided into three types according to syndrome differentiation: wind pestilence attacking exterior syndrome， pestilence-toxin obstructing lung syndrome， and toxin attacking lung and stomach syndrome， with the method of clearing pestilence and resolving toxin as the main treatment， combined with clearing exterior syndrome and clearing heat， dispersing lung and relieving cough， clearing stomach and stopping vomiting， and treated with Qingwen Jiebiao Decoction， Qingwen Xuanfei Decoction and Qingwen Anwei Decoction， respectively， and 65 children were clinically observed and achieved good results.
Professor Liu Shenlin's Experience in Treating Chronic Constipation
YE Ning-yuan
2019, 35(1): 109-110.
[Abstract](788) [PDF 1017KB](805)
Abstract:
Professor Liu Shenlin believes that the five zang-organs dominate the function of Pomen (anus)， so treatment of five zang-organs should be the way to cure constipation. Constipation due to deficiency syndrome should be given priority to with tonification and combination of purgation. Constipation due to excess syndrome should be based on purgation and pay attention to prevent injury. Through the analysis of diagnosis and treatment of typical medical records， the clinical experience of professor Liu Shenlin in treating chronic constipation was systematically summarized.
Efficacy Analysis and Application Prospect of Essential Oils from Chinese Medicine Based on Yin and Yang Theory
WUFeng-ye, FENGHan, WENYong-kun, DONGYun-fei, XIJiang-an, YAOJun-hong, DONGJie, CHENJun
2019, 35(1): 111-115.
[Abstract](788) [PDF 1073KB](699)
Abstract:
Theory of Drug Properties (TDP) is the core of the theory of traditional Chinese medicine(TCM). As major active substance of TCM， the application of essential oils （EOs) as penetration enhancers (PE) should be guided by TDP. In the present paper， based on the theory of Yin and Yang， as well as the understanding of drug properties of EOs， EOs are divided into three categories， such as "natures and flavours belong to Yang"， "natures and flavours belong to Yin" and "natures and flavours belong to Yin and Yang". Moreover， according to the literature analysis， it is found that the number of the papers of EOs with hot or warm natures and Pungent flavour in the treatment of cold syndrome is significantly more than those of the other two categories. Based on the knowledge of pungent flavour in TDP， such as "open skin slit" and "the pungent flaver related with four natures"， the application of EOs with "natures and flavours belong to Yang" as PE in transdermal formulations for cold syndrome is proposed， which is expected to make full use of the "Synergistic Effects" of EOs.
Research Progress of Chinese Medicine for Reinforcing Kidney and Activating Blood Circulation in the Treatment of Recurrent Miscarriage in Prethrombotic State
GUI Xiang-ling, LU Qi-bin
2019, 35(1): 116-120.
[Abstract](702) [PDF 1077KB](659)
Abstract:
This paper summarizes the treatment of recurrent miscarriage in the state before thrombus with Chinese medicine for kidney tonifying and blood circulation.Respectively from regulating hormone level， to improve the receptivity of endometrium， improve the microcirculation， regulating immune and inflammatory factors level four aspects discusses the kidney invigorate the circulation of traditional Chinese medicine treatment of thrombosis before state， the mechanism of action of recurrent miscarriage.This study provides a further basis for the treatment of recurrent miscarriage by Chinese herbal medicine.

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