2018 Vol. 34, No. 5

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Exploration of ZHANG Zhongjing's Lingzhu Formula
SHI Xin-de
2018, 34(5): 433-435.
[Abstract](4532) [PDF 328KB](3314)
Abstract:
From the aspects of drug composition， disease syndrome， etiology， pathogenesis and action sites， the prescriptions of Fuling (Poria) and Baizhu (Rhizoma atractylodis macrocephalae) (referred to as Lingzhu Formula) in Treatise on Cold Damage and Essentials from the Golden Cabinet were compared and combed. It is believed that the main treatment of Lingzhu Formula is a type of disease syndrome with abnormal water and liquid metabolism. According to the different positions of fluid retention， the composition of Lingzhu Formula is also different: If the fluid retention accumulated in the head， use Guizhi (Ramulus cinnamomi) Decoction removes Guizhi and add Fuling Baizhu Decoction. If the fluid retention accumulated in the epigastic， chest and rib-side， then use Linggui Zhugan Decotion， Fuling Zexie Decoction. If the fluid retention accumulated in the diaphragm， use Zhuling Powder; in the waist and abdomen， use Ganjiang Lingzhu Decoction; in the exterior and interior， use Wuling Powder. In addition， if the patient with yang deficiency and cold syndrome， it can use Zhenwu Decoction， Fuzi Decoction; with qi deficiency， use Lizhong and Fuling Decoction; with blood stasis， use Danggui Shaoyao Powder.
Reflections on Classification of TCM Treatment Methods
HUANG Yu-yan, ZHANG Li-ping, TANG Er-qun
2018, 34(5): 436-439.
[Abstract](2805) [PDF 355KB](1933)
Abstract:
There is no comprehensive strategy for classification of treatment methods， even the most classical classification: CHENG Guopeng's eight methods. The determination of treatment methods is a subjective speculation process. Treatment methods could be expressed in various ways， so the classification of treatment methods has the properties of subjectivity， limitation and multi-dimension. Starting with the TCM theoretical system， accompanied by related pathogenesis and etiology， it is more feasible to categorize the treatment methods. To solve the cross-repetition and tedious problem， treatment methods can be screened by determining the criteria concept and synonyms， selecting the smallest unit， and screening out the efficacy.
Discussion on Xiaoqinglong Decoction of Removing Ephedrae for Asthma
SONGDe-yin, SHISuo-fang
2018, 34(5): 440-442.
[Abstract](3130) [PDF 351KB](2066)
Abstract:
Combining with the viewpoints of the past doctors， this paper discusses the addition and subtraction of Xiaoqinglonng Decoction in the Treatise on Febrile Diseases， which "Removing Ephedra for asthma" is "in accordance with the meaning of ZHANG Zhongjing". And combing the relevant provisions in the Essentials from the Golden Cabinet to carry out a theoretical analysis and believes that it is excessively pursued and has no practical significance. According to Zhongjing's spirit of differentiation and treatment syndrome， Xiaoqinglong Decoction needs to distinguish between healthy qi and pathogenic qi relationship in specific clinical application. It can be used for patients with cold and good constitution， suitable for sweat method; if the patients with fluid retention and asthma， mild cold-exterior or accompanied with deficiency syndrome， it should not be used.
Effect of Jiubiying Decoction Combined with Chemotherapy on Serum Survinvin， p53 Protein and Immune Function in Elderly Advanced Non-Small Cell Lung Cancer
HUNai-qiang, LINCai-zhi, ZHAOHai-yan, ZHANGLin-ying, XUXue-lei, HANDan, SHAOWen-hui
2018, 34(5): 443-447.
[Abstract](2650) [PDF 445KB](1876)
Abstract:
OBJECTIVE To observe the clinical efficacy of Jiubiying Decoction combined with DP regimen (docetaxel+cisplatin) in the treatment of elderly patients with advanced non-small cell lung cancer. METHODS 100 elderly patients with advanced non-small cell lung cancer were randomly divided into treatment group and control group， 50 cases in each group. The control group was treated with DP chemotherapy， and the treatment group was given by Jiubiying Decoction on the basis of control group. All of them were treated with 21 days as a cycle and 4 cycles were observed. The changes of tumor size， TCM symptom score， side effects， serum p53， Survivin level and immune status were observed before and after treatment. RESULTS In terms of solid tumor changes， treatment group was superior to control group in total effective and disease control rate (P<0.05). After treatment， the TCM symptom scores， serum p53 and Survivin levels in two groups were lower than those before treatment (P<0.01)， the degree of reduction in treatment group was higher than that in control group (P<0.01). For immune function， the levels of CD3+， CD4+， CD4+/CD8+ in treatment group were significantly higher than those before treatment and control group (P<0.01). For side effects， the cases of myelosuppression and liver damage in treatment group were less than those in control group (P<0.05). CONCLUSION Jiubiying Decoction combined with DP regimen can better control tumor size， improve clinical symptoms， and decrease serum p53 and Survivin levels in elderly patients with advanced non-small cell lung cancer， and have synergistic and attenuating effects.
Clinical Study on Prevention and Treatment of Contrast-Induced Nephropathy with Baoshen Tablets in Perioperative Period of Coronary Intervention
MIAO Xiao-fan, WANG Fang, LIN Xin
2018, 34(5): 448-451.
[Abstract](2608) [PDF 384KB](1771)
Abstract:
OBJECTIVE To observe the effect and mechanism of Baoshen Tablets in preventing contrast-induced nephropathy after coronary intervention. METHODS 80 patients with coronary intervention were randomly divided into experimental group and control group. The control group was treated with standard basic treatment of coronary disease. The experimental group was given Baoshen Tablets， 4 tablets/time， 3 times a day on the basis of the control group. The serum creatinine (Scr)， urea nitrogen (BUN)， cystatin C (CysC)， glomerular filtration (eGFR)， urinary N-acetyl β-glucosaminidase (NAG) and urinary β-galactosidase level (GAL) levels were observed before surgery， 24 h and 72 h after surgery. RESULTS The comparison of the two groups in the same time showed that there was no significant difference in the levels of kidney injury markers before operation. After 24 h of operation， the level of CysC in experimental group was lower than that in control group (P<0.05). After 72 h of operation， the Scr， BUN， CysC， NAG， GAL in experimental group were lower than those in control group， while eGFR in experimental group was significantly higher than that in control group (P<0.05). The comparison of the same group in different time showed that CysC in control group after 24 h of operation was higher than the preoperative level (P<0.05). After 72 h of operation， the level of Scr， BUN， NAG， GAL were lower than the preoperative level (P<0.05)，while eGFR was higher than the preoperative leve in experimental group (P<0.05). In control group， after 72 h of operation， the levels of Scr， BUN， CysC， NAG and GAL were higher than the preoperative level (P<0.05)， and eGFR was lower than the preoperative level (P<0.05). CONCLUSION Baoshen Tablets can prevent and treat contrast-induced nephropathy for patients with the perioperative period of coronary intervention.
Impacts of Ningxin Anshen Formula on the Clinical Effects and Inflammatory Factors Levels of Patients with First-Episode Generalized Anxiety Disorder
XIONGHang, GUORong-juan
2018, 34(5): 452-455.
[Abstract](2809) [PDF 386KB](1778)
Abstract:
OBJECTIVE To explore the clinical effects of Ningxin Anshen Granules in the treatment of patients with generalized anxiety disorder (GAD) of blood deficiency and liver heat type， and its impacts on serum inflammatory factors. METHODS 60 patients with generalized anxiety disorder (blood deficiency and liver heat type) were selected. A randomized， double blind placebo control was used as control. The treatment course was 4 weeks. The observation group was intervened by orally taking Ningxin Anshen Granules， and the control group was intervened by orally taking placebo granules. Both groups were taking 1 dose/d， warm taking at morning and evening. Clinical effects， levels of inflammatory factors IL-1β， IL-6， TNF-α， interferon-γ (IFN-γ)， cortisol (CS) and prostaglandin E2 (PGE2)， and adverse reactions in two groups were observed and compared. RESULTS The total effect of the observation group was 83.33%， which was significantly higher than that of the control group (40%) (P<0.01). Comparing with the control group， the changes of IL-1β， IL-6， TNF-α， CS and PGE2 in the observation group were significant (P<0.05，P<0.01). However， there was no statistical difference in IFN-γ among groups(P>0.05). There was one case of adverse reactions in the observation group and two cases in the control group， and there was no significant difference between the two groups (P>0.05). CONCLUSION Ningxin Anshen Formula can effectively improve the anxiety symptoms of GAD patients， relieve inflammatory response with few adverse reactions and favorable compliance.
Effect of Shuangjiang Decoction on Carotid Atherosclerotic Plaque
ZHENGXiao-dan, XIEJin-hong, CAIJuan, ZHUJian-hua, JIANGWei-dong, NIWei-bing, TAOZhi-qiang, GAOXiang
2018, 34(5): 456-460.
[Abstract](2661) [PDF 423KB](1752)
Abstract:
OBJECTIVE To observe effect of Shuangjiang Decoction combined with atorvastatin on carotid atherosclerotic plaque. METHODS 107 patients with carotid atherosclerotic plaque were randomly divided into four groups. The basic treatment was based on low-salt and low-fat diet. The hard-plaque group 1 and soft-plaque group 1 were treated with oral atorvastation daily 20 mg， while hard-plaque group 2 and soft-plaque group 2 were given Shuangjiang Decoction on the basis of 20 mg of atorvastation. One dose per day orally， each group was continuously taken for 12 weeks. The serum monocyte chemoattractant protein-1 (MCP-1)， macrophage migration inhibitory factor (MIF)， matrix metalloproteinase-1 (MMP-1)， matrix metalloproteinase-9 (MMP-9)， nuclear factor-κBp65 (NF-κBp65)， transforming growth factor-β1(TGF-β1) levels， plaque area and TCM syndrome scores of two groups were observed before and after treatment. RESULTS High levels of cellular inflammatory factors MCP-1， MIF， NF-κBp65， TGF-β1 and proteases MMP-1 and MMP-9 were observed in the serum of each group. Compared with hard-plaque group 1 and 2 with Shuangjiang Decoction before treatment， the level of MCP-1， MIF， NF-κBp65， MMP-1， MMP-9 in soft-plaque group 1 and 2 with Shuangjiang Decoction were higher， while TGF-β1 levels were decreased (P<0.01). Compared with the pre-treatment group， the level of MCP-1， MIF， NF-κBp65， MMP-1， MMP-9 in the serum of hard-plaque group 1 and soft-plaque group 1 were increased， while the level of TGF-β1 was decreased (P<0.05). The changes of hard-plaque group 2 and soft-plaque group 2 with Shuangjiang Decoction were especially significant(P<0.01). Compared with hard-plaque group 2 with Shuangjiang Decoction after treatment， the levels of MCP-1， MIF， NF-κBp65， MMP-1 and MMP-9 in soft-plaque group 2 with Shuangjiang Decoction were increased， while the level of TGF-β1 was decreased (P<0.05). Compared with pre-treatment， the area of carotid plaque in group 1 of hard-plaque and soft-plaque group 1 was reduced (P<0.05)， and in group 2 of hard-plaque and soft-plaque with Shuangjiang Decoction was also reduced (P<0.01). The area of the cartoid plaque in soft-plaque group 2 with Shuangjiang Decoction was better than that in hard-plaque group 2 with Shuangjiang Decoction (P<0.05). After treatment， each group can reduce the TCM syndrome scores. Compared with the hard-plaque group 1 and soft-plaque group 1， the hard-plaque group 2 and soft-plaque group 2 with Shuangjiang Decoction can significantly reduce TCM syndrome scores in patients with carotid atherosclerosis (P<0.05). CONCLUSION Cytoinflammatory factors MCP-1， MIF， NF-κBp65， TGF-β1 and proteases MMP-1 and MMP-9 are involved in the regulation of carotid atherosclerosis. Shuangjiang Decoction and atorvastatin can effectively reduce carotid plaque area and significantly improve carotid plaque. Patients with TCM syndromes， the combination of the two is more effective， and its mechanism may be related to lowering serum MCP-1， MIF， MMP-1， MMP-9， NF-κBp65 levels and increasing serum TGF1 levels.
Clinical Study on ZiShen Jianpi Formula Combined with Lymphocyte Immunotherapy for Recurrent Abortion of Deficiency of Both the Spleen and Kidney
SONGYan-li, WEIAi-wu, SUNZi-xue, MENBo, ZHANGHua
2018, 34(5): 461-464.
[Abstract](2619) [PDF 369KB](1753)
Abstract:
OBJECTIVE To observe the efficacy and safety of Zishen Jianpi Formula combined lymphocyte immunotherapy for recurrent abortion of deficiency of both the spleen and kidney syndrome. METHODS 120 patients with recurrent abortion of deficiency of both the spleen and kidney were randomly divided into control group and experimental group， 60 cases in each group. The control group was treated with lymphocyte immunotherapy， and the experimental group was given Zishen Jianpi Formula on the basis of the treatment of control group. The TCM syndrome scores， serum blocking antibodies (CD3+， CD4+， CD8+， CD4+/CD8+) and inflammatory factors (IFN-γ， TNF-α， IL-1β， IL-10) were observed. The effective rate and adverse reaction rate of the two groups were compared. RESULTS 3 patients in control group and 2 patients in experimental group were fell off during the study. The term birth rate of experimental group was 89.7%， which was higher than that of control group 71.9%(P<0.05). The total effective rate of experimental group was 94.8%， which was higher than that of control group 80.7% (P<0.05). The TCM symptom scores， serum blocking antibody and inflammatory factor levels in experimental group were higher than the control group (P<0.05). The incidence of adverse reaction in treatment group was 10.3% (6/58)， which was lower than that of control group 28.1% (16/57) (P<0.05). CONCLUSION Zishen Jianpi Formula combined with lymphocyte immunotherapy for recurrent abortion of deficiency of both the spleen and kidney syndrome can significantly improve the rate of full-term delivery. The mechanism may be related to improving blood coagulation function， regulating serum blocking antibody and inflammatory factor levels.
Effect of Invigorate Qi and Warm Yang Formula on Myelosuppression in Patients with Advanced Non-Small Cell Lung Cancer after Chemotherapy
LUCheng-jie, GAOJing-dong, LIUMin, CHENTing, CHENJia-lu, YINPing
2018, 34(5): 465-469.
[Abstract](2602) [PDF 410KB](1737)
Abstract:
OBJECTIVE To observe the efficacy of invigorate qi and warm yang formula on myelosuppression in patients with advanced non-small cell lung cancer. METHODS 60 patients with lung and spleen (qi) yang deficiency syndrome of non-small cell lung cancer were randomly divided into experimental group and control group， 30 cases in each group. The control group was treated with conventional chemotherapy， and the experimental group was treated with invigorating qi and warming yang formula on the basis of control group， until the end of second stage chemotherapy. The blood routine， liver and kidney function were compared between two groups on first day before chemotherapy， the second day and the fifth day after chemotherapy. The changes of TCM symptoms， quality of life， related tumor indicators and immune function indexes were compared before and after chemotherapy. RESULTS The incidence of severe inhibition of bone marrow in experimental group was lower than that in control group during the treatment period (P<0.05). The improvement of quality of life of experimental group was superior to the control group (P<0.05). The experimental group has certain advantages in improving the symptoms of TCM and specific tumors of certain lung cancers compared with the control group (P<0.05). CONCLUSION Invigorating qi and warming yang formula combined with chemotherapy in the treatment of advanced non-small cell lung cancer can significantly reduce the degree of myelosuppression and effectively improve the clinical symptoms of patients， the stability of disease treatment， the quality of life， and cause less adverse reactions.
Clinical Study on the Self-Made Paishi Decoction Combined with Acupuncture for Relieving the Postoperative Inflammatory Reaction of Upper Urinary Calculi
HUANGTing, ZHUXiao-yu, SONGRi-jin, CAOPu, SUShi-feng, WANGAn-xi
2018, 34(5): 470-473.
[Abstract](2559) [PDF 406KB](1809)
Abstract:
OBJECTIVE To observe the clinical effect of self-made Paishi Decoction combined with acupuncture for relieving the postoperative inflammatory reaction of upper urinary calculi. METHODS 60 patients who have received upper urinary calculi operation were randomly divided into the experimental group and the control group， 60 cases in each group. The experimental group was treated with self-made Paishi Decoction combined with acupuncture， and the control group was treated with western medicine. After four weeks of operation， the clinical effect of both groups were evaluated， the Urinary Stent Symptom Questionnaire (USSQ) were completed and the levels of serum inflammatory factor hypersensitivity C reactive protein (hs-CRP)， interleukin 6 (IL-6)， tumor necrosis factor alpha (TNF-α)， urine oxalic acid， urinary calcium， urinary glucan and urine citric acid levels in both groups were detected. RESULTS Four weeks after the operation， TCM symptom score of the experimental group was significantly lower than that in the control group (P<0.05). Scores of the urinary tract symptoms， body aches and USSQ， levels of hs-CRP， IL-6， TNF-α， urine oxalic acid and urinary calcium levels in the experimental group were obviously lower than those in the control group (P<0.01)， urine citric acid level was higher than that in the control group (P<0.05). Three moths after the operation， there were 58 cases and 52 cases in the experimental and the control group respectively completely discharged the residual stones (P<0.05). CONCLUSIONS Self-made Paishi Decoction combined with acupuncture can effectively improve the postoperative inflammatory reaction of upper urinary calculi. This treatment method has obvious effect for discharging the residual stones， which is worth further clinical application.
Comparative Study on the Transdermal Penetration Enhancement Effect of Essential Oils of Interior-Warming Drugs Based on Electrical Resistance of Skin
RENLüe, CHENJian-ya, YAOJun-hong, ZHUXue-min, DONGJie, GUWei, CHENJun
2018, 34(5): 474-479.
[Abstract](2718) [PDF 808KB](1816)
Abstract:
OBJECTIVE To evaluate and establish a method for rapid comparison of transdermal penetration enhancement effect of essential oils （EOs） from Chinese materia medica (CMM). METHODS  The active components （ferulic acid，albiflorin，paeoniflorin，tetrahydrocolumbamine，tetrahydropalmatine） in Xiangfu Siwu Decotion （XFSWD）were used as model drugs for transdermal permeation study. The oil/water partition coefficient values of the above active components were measured. Six EOs of interior-warming drugs were used as penetration enhancer （PE）. The Franz diffusion cell method was applied to investigate the effects of EOs on transdermal permeation of active components in XFSWD. The impact of EOs on skin integrity was evaluated by electrical resistance （ER） of the skin. Finally，the correlation between the results of skin resistance dynamics and in vitro transdermal permeation experiment was assessed. RESULTS The oil-water partition coefficients of ferulic acid，albiflorin，paeoniflorin，tetrahydrocolumbamine，tetrahydropalmatine were measured to be -0.93，-0.59，-0.38，1.97， 2.15. Six EOs of interior-warming drugs as PE could significantly enhance the permeation of active components in XFSWD，especially the polar components. The EOs with hot nature possess better penetration ability than that of warm nature. Besides，there was a good correlation between the results of skin resistance dynamics and in vitro transdermal permeation experiments for above drugs with different polarity(r=0.748 1-0.812 9). CONCLUSION The penetration enhancing effect of EOs of interior-warming drugs are affected by drug four nature property characteristics of CMM.The ER method can effectively identify the penetration enhancing effect of EOs.
An Investigation Into the Effect of Process Parameters of Sedi Herba<\i> Extract Granulation in Fluid Bed on Granule Size Distribution by Response Surface Model and Partial Least Squares Model
HU Ya-jing, WANG Gang, LUO Ying, LI Ya-fang, YI Cheng
2018, 34(5): 480-484.
[Abstract](2628) [PDF 909KB](1874)
Abstract:
OBJECTIVE To explore the particle size distribution of granules prepared by fluid bed granulation via response surface regression model (RSM) and partial least square regression model (PLS). METHODS Sedi Herba extract was granulated by fluid bed granulation. Box-Behnken design in RSM was utilized to study the effects of binder addition rate (X1)， the liquid to solid ratio (X2) and air temperature (X3) on particle size distribution. Moreover， RSM and PLS were employed to explore the influence of process parameters on particle size distribution. RESULTS The results demonstrated that both of RSM and PLS could fit the fluid bed granulation well. Furthermore， RSM model exhibited better model fitting precision and prediction ability. CONCLUSION We could understand the process of fluid bed granulation profoundly based on experimental design and the different statistical models. A robust and high prediction model could be achieved， which could provide reliable basis and technical support for further production.
Effect of Modified Yam Pills on Serum Specific MicroRNAs Expression in Patients with Mild and Moderate Alzheimer's Disease
XIEWen-ting, TANZi-hu, CHENYan, LIXian-wei, LIUHong-han
2018, 34(5): 485-490.
[Abstract](2652) [PDF 484KB](1926)
Abstract:
OBJECTIVE To explore the expression of serum microRNAs (miRNAs) in patients with mild and moderate Alzheimer's disease and the effect of modified Yam Pills on it. METHODS The literature of peripheral blood miRNAs in AD patients was retrieved in Pubmed and China National Knowledge Internet database. The miRNAs with consistent expression in multiple literatures were selected for the study. Totally 56 patients with mild and moderate Alzheimer's disease were treated with modified Yam Pills of 15 mL each time， twice a day for 12 weeks. Another 56 cases with normal cognitive function were collected as control group. The serum miRNAs levels of AD patients before and after treatment and control group were measured by fluorescence quantitative PCR. RESULTS Compared with the control group， the expression of serum miR-107， miR-29a， miR-29b， miR-181c， miR-125b， miR-135a and miR-342-3p were significantly decreased in the AD group， and the expression of miR-146a was significantly increased (P<0.01). Compared with those before treatment， the expression of serum miR-107， miR-29a， miR-29b， miR-181c， miR-125b and miR-342-3p were significantly increased after treatment in the AD group (P<0.05， P<0.01)， and the expression of miR-146a was significantly decreased (P<0.01). CONCLUSION Detecting the expression of serum miR-107， miR-29a， miR-29b， miR-181c， miR-125b， miR-135a， miR-342-3p and miR-146a may be helpful to the early diagnosis of AD patients. Modified Yam Pills may play a role in the treatment of AD by regulating the levels of serum miR-107， miR-29a， miR-29b， miR-181c， miR-125b， miR-342-3p and miR-146a in AD patients.
Immune Effects of Tripterine on Collagen Induced Arthritis Model Mice
LUJia-wei, CHENDu, ZHANGShu-fang, BIANYong, LIULi, ZHANGJian, LIUShi-jia
2018, 34(5): 491-494.
[Abstract](2712) [PDF 858KB](2041)
Abstract:
OBJECTIVE To study the anti-inflammatory effect of tripterine on bovine type Ⅱ collagen-induced arthritis in mice and its mechanism of action on T cells. METHODS DBA/1J mice were used to induce CIA model induced by bovine type Ⅱ collagen. After the model was established， tripterine of 2 mg/kg was given to the mice and the joint score was recorded. The mice were sacrificed after 70 d of secondary immunization. The levels of IL-17， IL-6 and TGF-β in the plasma of mice were detected by ELISA. Pathological changes of the joints were observed by HE staining. Flow cytometry was used to detect the ratio of Th17 to Treg in mouse spleen cells. RESULTS The joint scores of tripterine in mice were significantly lower than that in model group (P<0.05). HE staining showed that tripterine improved the synovitis infiltration (P<0.05)； ELISA showed that tripterine significantly decreased the levels of Th17-associated cytokine IL-6 (P<0.01). Flow cytometry showed that tripterine increased the proportion of Treg cells (P<0.05) while significantly decreased the proportion of Th17 cells in the spleen of mice (P<0.01). CONCLUSION  Tripthenin can effectively alleviate the symptoms of joint inflammation in CIA mice， inhibit synovitis inflammatory cell infiltration， angiogenesis and cartilage destruction. Tripterine treatment of CIA mice inflammation may be through the reduction of IL-6 secretion in CIA mice with anti-inflammatory cytokines involved in the immune balance regulation process.
Mechanismof Cordycepin Promotes Tumor Immunity by Regulating PD-1 Receptor of CD4+T Lymphocytes
ANQing, HELi-wei, WUHong-yan, HOUXian-bang, ZHANGHong-jiang, ZHUAn-hong, ZHANGXiao-ping, FANFang-tian
2018, 34(5): 495-498.
[Abstract](2792) [PDF 607KB](1783)
Abstract:
OBJECTIVE To investigate the mechanism of killing effect of cordycepin on tumor-associated CD4+T lymphocyte tumor in vitro. METHODS Tumor-associated CD4+T-lymphocytes were isolated from xenograft tumors. MTS assay was used to study the effect of cordycepin on the proliferation of tumor-associated CD4+T-lymphocytes; LDH method was used to analyze the killing effect of tumor-associated CD4+T lymphocytes treated with cordycepin. For the co-incubation of tumor cells， the cytotoxicity of the supernatants of the cells incubated with CD4+T lymphocytes and tumor cells was detected by ELISA， and Western blot was used to study the related protein in the PI3K/AKT pathway. RESULTS Cordycepin could promote the proliferation and killing effect of tumor-associated CD4+T lymphocytes in a dose-dependent manner， and cordycepin reduced PD-1 protein and enhanced PI3K/AKT pathway-associated protein expression and promote IL-2 and IFN-γ expression in tumor-associated CD4+T lymphocytes. CONCLUSION Cordycepin can promote the proliferation and killing effect of tumor-associated CD4+T lymphocytes and its mechanism may be related to the inhibition of tumor-immune checkpoint PD-1.
The Effects of Geniposide on Th17/Treg Balance and Local Inflammatory Factors in Rats with Rheumatoid Arthritis
YUYun, SHILe, YUBin, BIANYong
2018, 34(5): 499-503.
[Abstract](2633) [PDF 845KB](1770)
Abstract:
OBJECTIVE To investigate the effects of geniposide on Th17/Treg balance and local inflammatory factors in rats with rheumatoid arthritis. METHODS We established the CIA model to observe the histopathological changes of ankle joints in rats. ELISA was used to detect plasma TNF-α， and Western blot was used to detect the protein contents of transcription factor Foxp3 and RORγt. The expression of IL-10 and IL-17 in joint synovial tissue was detected by immunohistochemistry. RESULTS Compared with the normal control group， the serum concentration of TNF-α， IL-17 and ROR-γt and the expression of IL-17 in joint synovial tissue were significantly higher in the model group (P<0.01)， while the concentration of IL-10 and Foxp3 and the expression of IL-10 in joint synovial tissue decreased significantly in the model group (P<0.01). Compared with the model group， the serum concentration of TNF-α， IL-17 and ROR-γt and the expression of IL-17 in joint synovial tissue were significantly lower in the middle- and high-dose groups of geniposide (P<0.05， P<0.01)， while the concentration of IL-10 and Foxp3 and the expression of IL-10 in joint synovial tissue increased significantly in the middle- and high-dose groups of geniposide (P<0.05， P<0.01). CONCLUSION There was an imbalanced Treg/Th17 differentiation in the joint synovium of CIA rats. By inhibiting the differentiation of Th17 cells in joint synovium， geniposide might induce the generation of Treg cells and then restore the Treg/Th17 balance in the diseased area.
Therapeutic Compatibility Effect of Glycyrrhizin and Osthole on the Treatment of Alcoholic Fatty Liver in Rats
YUNFei, KANGAn, DILiu-qing, LIURuo-nan, XUXiao-yan
2018, 34(5): 504-509.
[Abstract](2674) [PDF 1011KB](1760)
Abstract:
OBJECTIVE To study the effect of glycyrrhizin acid and osthole on the treatment of alcoholic fatty liver in rats， and to explore the mechanism of compatibility of components. METHODS After the establishment of rat alcoholic fatty liver， the rats were randomly divided into normal group， model group， positive drug group (Lipingzhi)， osthole (Ost) group， glycyrrhizic acid (GL) group， high dose group of osthole and glycyrrhizic acid， medium dose group of osthole and glycyrrhizic acid and low dose group of osthole and glycyrrhizic acid. The treatment groups were given by gastrogavage every day starting from the 5th week， the positive group ig. Lipingzhi 20 mg/(kg·d)， the osthole group ig. Ost 10 mg/(kg·d)， the glycyrrhizic acid group ig. GL 22.5 mg/( kg·d)， the low dose group ig. the compatibility components of Ost 5 mg/(kg·d) and GL 11.25 mg/(kg·d)， the medium dose group ig. Ost 10 mg/(kg·d) and GL 22.5 mg/(kg·d)， the high dose group ig. Ost 20 mg/(kg·d) and GL 45 mg/(kg·d) and the normal and model group were given distilled water with equal volume for 6 weeks. We observed the sign of rats， the pathological changes of liver with the optical microscope. Then we measured AST， AKP and ALT changes in rat serum and TC， TG， SOD and MDA contents in liver homogenate. The expression of PPARα protein in liver tissue was examined using Western blot method. RESULTS Compared with the model group， the pathological changes of liver tissue were improved in different treatment groups and the ALT， AST and AKP levels in serum were significantly decreased in the groups of glycyrrhizin combined with osthole at medium and high doses(P<0.05). The liver tissue of rats test results showed the medium dose and high dose groups of glycyrrhizin combined with osthole could significantly reduce the content of MDA and improve the activity of SOD. There were significant differences among the model group and the treatment groups at medium and high doses (P<0.01). The TG and TC levels in the group of glycyrrhizin combined with osthole in medium dose were lower than the model group(P<0.05). Western blot results showed that the PPARα protein levels in treatment groups compared with the model group had a different degree of increase， and the compatibility of medium dose and high-dose groups in the liver tissue of PPARα protein expression was up-regulated with statistical significance (P<0.01). CONCLUSION The reasonable combination of glycyrrhizic acid and osthole had synergistic treatment on alcoholic fatty liver， and its main mechanism might be related to the improving of liver function， regulation of fat metabolism， anti-lipid oxidation and the increased protein expression of PPARα.
Influence of Liangxue Tongyu Fang on Proliferation， Secretion and Related Signaling Pathway Proteins Expression in VEC
ZHAO Feng-ming, HUANG Yan, LI Guo-chun
2018, 34(5): 510-512.
[Abstract](2536) [PDF 1364KB](1995)
Abstract:
OBJECTIVE By studying the effects of Liangxue Tongyu Fang on proliferation， secretionand and related signaling pathway proteins expression in VEC， to discuss the mechanism of the hemorrhagic stroke. METHODS VEC were cultured in vitro ，H2O2 at 1 mmol/L was used to induce oxidative stress injury model in endothelial cells. MTT was used to detect cell proliferation activity，Inverted microscope was used to observe the cell morphology， Hochst staining was used to examine apoptosis. Chemical method were used to detect the levels of NO， TNOS and iNOS in supernatant cells.Western blot method were used to detect the expression of NF-κB， P53， caspase-3 and P21 protein. RESULTS Compared with the H2O2 model group， Liangxue Tongyu Fang at high， medium and low dose groups obviously increased the proliferation activity and elevated levels of NO，TNOS and iNOS in the cell supernatant. The medium dose group improved cell morphology， reduced apoptosis and the expression of NF-κB， P53， caspase-3 and P21 proteins. CONCLUSION By promoting cell proliferation， inhibiting cell apoptosis， increasing the secretion of NO and the activity of TNOS and iNOS， reducing the expression of NF-κB， P53， caspase-3 and P21 signaling pathway proteins， thus protecting vascular endothelial cells is the mechanisms for the treatment of hemorrhagic stroke.
Experience of Nephropathy by National Chinese Medicine Master ZOU Yanqin of "Fom up Treat down Disease" in Clearing Throat
ZHANGWen-xi, YILan
2018, 34(5): 513-515.
[Abstract](3028) [PDF 357KB](2090)
Abstract:
The throat is closely related to the kidney. National Chinese Medicine of ZOU Yanqin has created the theory of treating nephropathy with "from up treat down disease" in the treatment according to pattern differentiation， and pays attention to clearing throat at the same time. The symptom of throat at acute stage are given to disperse wind and discharge heat， clear heat from throat to dispel evils， and treat the branch and prevent evil heat transmission to kidney. Supplementing and boosting the lung and kidney， nourishing yin and clearing throat at remission stage to support right and treat the root.
Warming Meridian and Dredging Collateral in Treating Wind-Cold-Dampness Arthralgia Syndrome of Orthopedics
MINWen, TANFeng, CHENGZhou, TANDeng, HANLong, FANGPeng-hua, HUANGGui-cheng
2018, 34(5): 516-519.
[Abstract](2983) [PDF 391KB](2196)
Abstract:
The wind-cold-dampness arthralgia syndrome is quite common in orthopedics. It believes that the external cause of the syndrome is intermingled of pathogens evils， and the internal cause is morbid Yang qi and evil lies in the body meridians. Morbid Yang qi of spleen and kidney is especially important in the pathogenesis of the disease. The treatment should be based on warmth， which can warm spleen yang， resolve constraint and calm the mind， warm kidney yang and nourish kidney yin. The meridian block leads to disharmony of qi and blood and malnutrition sinew and bone， and dredging meridian is also an important treatment. The dredging therapy can be based on the specific syndrome and its pathogenesis， or dispel pathogen or reinforce healthy qi， or combined or focus on one side. Warming meridian and dredging collateral in treating wind-cold-dampness arthralgia syndrome of orthopedics， should consider the individual differences in age， physical constitution， disease course， disease position， etc. The prescriptionis certified by the symptom， and the medicine is compatible with the symptom.
Professor SHAN Zhaowei's Experience in Treating Chronic Diarrhea
XUSu-su, ZHANGXiao-qin
2018, 34(5): 520-523.
[Abstract](3425) [PDF 403KB](2457)
Abstract:
This paper summarizes professor SHAN Zhaowei's experience in treating chronic diarrhea. He believes that the spleen deficiency and exuberant phlegm is the root， while dampness-heat， static blood and accumulated food is the branch. The disease is in the spleen and stomach， involving in liver and kidney. According to the characteristics of diarrhea to identify the asthenia and sthenia， cold and heat， viscera of spleen， liver， kidney and flexible combination of prescriptions. Meanwhile professer SHAN is good at treating chronic diarrhea by using wind medicine and combining warm medicine with cold medicine. When taking care of other pathological factors， it is taken into account the methods of digestion， invigorating blood and resolving stasis. Pay attention to tongue diagnosis and tongue syndrome.
Progress on Effective Components of Traditional Chinese Medicine Indiabetic Nephropathy
WANG Ling, LI Yu, GU Chun-yan
2018, 34(5): 524-528.
[Abstract](2690) [PDF 509KB](1794)
Abstract:
Diabetic nephropathy (DN)， as a serious complication of type 2 diabetes mellitus (T2DM)， is the major cause of end-stage renal disease with high mortality worldwide. The progressive kidney disorder is pathologically characterized by glomerular fibrosis， thickened glomerular and tubular basement membranes， mesangial cells expansion， accumulation of the extracellular matrix and increased mesangial hypertrophy. Although the exact causative factor of DN remains unclear， several mechanisms have been postulated， such as oxidative stress， increased inflammation， renal hyperfiltration and renal injury. Therefore， lots of synthesized drugs were developed to focus on these therapeutic targets to cure DN. However， clinical severe side effects have emerged following the treatments. Traditional Chinese medicine has showed the advantages to remedy DN for its natural and safe character. This review will highlight the classic and effective components of traditional Chinese medicine to cure diabetic nephropathy in preclinical researches to offer new therapeutic strategies for an adequate pharmacotherapy of DN.
Research Progress on Anti-Non-Small Cell Lung Cancer Mechanisms of Natural Chinese Medicine Monomer and Its Derivatives by MiRNAs Regulation
JIANGZe-qun, WUMian-hua, YANGYe
2018, 34(5): 529-534.
[Abstract](2743) [PDF 506KB](1801)
Abstract:
Non-small cell lung cancer (NSCLC) is one of the most serious threats to human beings. There is an urgent need to develop new anti-cancer therapies. With the deepening of the research on the anticancer activity of natural Chinese medicine， miRNAs research on the growth， proliferation， invasion， migration and drug sensitivity of NSCLC cells by regulation of natural Chinese medicine monomer and its derivatives has been paid more and more attention. This paper gives an overview of the latest research progress on the anti-NSCLC effects of natural medicine components and derivatives via miRNAs regulation， which aims to provide new ideas and references for the development of NSCLC drugs.
Current Situation and Analysis of Acupuncture Metabonomics
ZHANGHong-ru, TAOJia-lei, XUSen-lei, LUSheng-feng, NIGuang-xia
2018, 34(5): 535-540.
[Abstract](2721) [PDF 438KB](2047)
Abstract:
Acupuncture as a means of prevention and treatment of diseases， is widely used in clinical practice. Because of the complexity of the human body， the mechanism of acupuncture is not yet clear. The application of metabonomics can provide valuable information for the rule of acupuncture effect and biological basis， and it is of great significance for explaining acupuncture theory， promoting the modernization and internationalization of acupuncture and moxibustion. After analyzing and summarizing the current research situation of acupuncture metabonomics in recent ten years， the author puts forward some deficiencies and ideas in order to promote the development of acupuncture research.

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