南京中医药大学学报

Discussion on the Guidance Value of Heart-Kidney Concept in Gynecology and Clinical Practice

XIA Gui-cheng, TAN Yong

2017, 33(6): 541-544.

[Abstract](1080) [PDF 414KB](1449)

Abstract:
As the core theory of TCM， viscera theory always guiding the clinical practice. This paper presented the view of heart-kidney-uterus axis， and analyzed the relationship between heart-kidney and female physiology and pathology. Female periodic rhythm was expounded from the view of heart and kidney. The movement form of heart-kidney and their alteration in female menstruation and reproductive cycle was discussed. Therefore， the clinical treatment concept of mainly regulating heart and kidney is put forward， which reveals the core of the regulation of life rhythm.

Discussion on Pelvic Inflammatory Disease Treatment Characteristics of TCM Master Xia Guicheng

TAN Yong, XIA Gui-cheng, CHEN Jie, HU Rong-kui, XIA Yun

2017, 33(6): 545-546.

[Abstract](1175) [PDF 971KB](1145)

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This paper introduces the pelvic inflammatory disease treatment characteristics of TCM Master Xia Guicheng. He emphasizes that comprehensive regulation is needed for pelvic inflammatory disease needs， and clearing heat and detoxifying， removing dampness and blood stasis are not the only methods for regulating internal disease resistance. For the acute stage， the treatment should be based on blood-heat of lower Jiao， clearing heat and detoxifying， removing blood stasis to stop pain should be used. For the chronic stage， reinforcing healthy qi should be taking as the root treatment which advocates menstrual cycle therapy under the regulation of heart-kidney-uterus axis， and heart-kidney interaction， removing blood stasis to stop pain should be used.

TCM Master Xia Guicheng's Six Treating Methods for Dysmenorrhea

HU Rong-kui, TAN Yong, YIN Yan-yun, CHEN Jie, XIA Yun

2017, 33(6): 547-550.

[Abstract](1065) [PDF 999KB](1838)

Abstract:
This paper expounds the concept and method of TCM Master Xia Guicheng diagnosis and treatment of dysmenorrhea， which is summed up as six kinds of methods. Master Xia believes that the cause of dysmenorrhea is generally attributed to qi stagnation or blood stasis， and the general treatment usually performs from six aspects including stopping pain， dredging channels， calming the heart， regulating liver， warming meridian and spasmolysis， which have achieved remarkable effects. The treatment root is focus on regulating the activities of yin-yang， qi and blood of the menstrual cycle rhythm， and to treat dysmenorrhea by improving female's imbalance of the body environment through balancing yin and yang， smoothing qi and blood circulation.

Philosophy and Method of Regulating and Treating PCOS by TCM Master Xia Guicheng

CHEN Jie, TAN Yong, REN Qing-ling, HU Rong-kui

2017, 33(6): 551-553.

[Abstract](1146) [PDF 1045KB](1144)

Abstract:
This paper introduces the philosophy and method of regulating and treating polycystic ovary syndrome (PCOS) by TCM master Xia Guicheng. Circular motion biological clock rhythm of female reproductive axis was analyzed with the concept of waxing and waning， conversion of yin and yang in Tai Chi of Yi-ology. He proposes heart-kidney interaction， priority of balancing yin and yang， coordination of liver and spleen， qi and blood， and regulating the changes of menstruation cycle to treat menstrual disorder. It is believed that the disorder of the circular motion biological clock rhythm of reproductive axis is the source of PCOS. Dysfunction of main zang-fu organs is the key of PCOS， including lack of essence-generation source due to the insufficiency of reproduction-stimulating essence， rhythm disorder due to non-interaction between the heart and the kidney， yin deficiency with yang hyperactivity due to insufficiency of liver-yin， phlegm and fat causing disease due to spleen and stomach. The dysfunction of storing and discharging of extraordinary fu organs is the apparent factor of menstruation disorder in PCOS.

Effect of Bushen Zhuyun Formula on Pituitary GnRH Receptor and Signal Transduction System in LPD Rats

ZHOU Bo-ru, ZHOU Hui-fang, LIU Bei, ZHU Hong-kun, DAI Jia-xuan, XU Jian-ya, XIE Tong

2017, 33(6): 554-559.

[Abstract](1047) [PDF 1701KB](1134)

Abstract:
OBJECTIVE To explore the effect and mechanism of Bushen Zhuyun formula(BSZYF) on gonadotropins and gonadotropin-releasing hormone receptor(GnRHR) signaling transduction in mifepristone-induced LPD rats， and to find the scientific evidence of "heart， kidney and uterus axis" theory of TCM. METHODS SD female rats were administered mifepristone suspension via oral gavage， followed by pharmaceutical intervention. Then， the serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected by ELISA assay， the FSHβ and LHβ expression levels in the pituitary were determined by qPCR， and the GnRHR expression levels were assessed by qPCR and Western blot analysis. Simultaneously， transcription factors， such as c-Jun， Elk-1， Egr-1， Nur77 and CREB， and transduction molecules in PKC-MAPK， Ca 2+ -CAM， and cAMP-PKA signaling pathways， such as PKC， p38MAPK， ERK1/2， CAM， and PKA were assessed by qPCR and Western blot analysis. RESULTS After pharmaceutical intervention， BSZYF increased the serum FSH levels suppressed by mifepristone (P<0.05). Moreover， the expression of c-Jun， Elk-1， Egr-1， CREB， PKA， p38MAPK， JNK， ERK and PKA were was significantly upregulated in the BSZYF group compared with that in the mifepristone group (P<0.05). However， there was no statistical difference in Nur77 and CAM expression among the blank， mifepristone and BSZYF groups (P>0.05). CONCLUSION BSZYF can regulate abnormal pituitary gonadotropin secretion and GnRHR signaling transduction systems induced by mifepristone， which confirms that the prescription can regulate the whole function of the reproductive axis， and explains the consistency of "Heart， kidney and uterus axis" theory in TCM and the concept of the hypothalamus-pituitary-ovary axis in modern medicine

Clinical Effects of TCM Diagnosis and Treatment Project on Patients with MODS Gastrointestinal Dysfunction of Abdominal Distension and Fullness Disease

ZHOU Jiang, CHEN Ming-qi, GENG Yan-xia, PEI Ying-hao, LU jun, DAI Lin-feng, LYU Hai, WANG Xing

2017, 33(6): 560-564.

[Abstract](1012) [PDF 1034KB](1183)

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Clinical Study on Differences of Gastric Receptivity and Emptying Function of Functional Dyspepsia Patients with Different Syndromes

SHIZhao-hong, FULi-he, ZHAOLei, HUWei, YANGJian, GUOJie, TUBei-lei, LIUFan

2017, 33(6): 565-569.

[Abstract](959) [PDF 1033KB](944)

Abstract:
OBJECTIVE To study the differences of gastric receptivity and emptying function of functional dyspepsia (FD) patients with different syndromes by liquid nutrient load test combined with B ultrasound examination. METHODS Seventy four subjects were included， which included 14 healthy cases (HS group)， 60 FD patients (33 cases of spleen deficiency and qi stagnation， 16 cases of spleen deficiency and dampness accumulation， 11 cases of spleen yang deficiency). Liquid nutrient loading test was performed for all subjects， and the threshold and maximum intake were recorded combined with VAS visual score. Gastric emptying rate and half emptying time of patients with different syndromes were compared by ultrasonic measurement. RESULTS The maximum intakes of three syndromes in FD group were lower than that in the HS group (P<0.05~0.01)， and the maximum intake of spleen deficiency and dampness accumulation syndrome was lower than those of spleen deficiency and qi stagnation syndrome and spleen yang syndrome (P<0.05). Compared three syndromes in FD group with HS group， distal gastric emptying rate of postprandial 90min of spleen yang deficiency syndrome was lower than that of HS group (P<0.05). distal gastric emptying time of spleen deficiency and dampness accumulation syndrome was longer than that of spleen deficiency and qi stagnation syndrome and spleen yang deficiency syndrome in HS group (P<0.05). CONCLUSIONS There is difference in gastrointestinal motility between FD patients with the same syndrome， and that of the patients with spleen deficiency and dampness accumulation syndrome decreased more significant， which provides theoretical basis for TCM syndrome differentiation and treatment.

Study on the Curative Effect of Qigui Yishen Decoction in the Treatment of Patients with Chronic Kidney Diseases of Spleen and Kidney Deficiency

WEIMing-gang, HEWei-ming, LIFeng-ling, ZHANGLing, FEIMei, XIONGPei-hua, SUNWei

2017, 33(6): 570-573.

[Abstract](965) [PDF 1019KB](1245)

Abstract:
OBJECTIVE To study the clinical curative effect of Qigui Yishen decoction in the treatment of patients with chronic kidney diseases of spleen and kidney deficiency.METHODS Patients with chronic kidney diseases met the diagnosis criteria were divided into two groups. Both groups were given the high quality low protein， low salt， low fat， low phosphorus diet， and also given basic treatments such as correcting water and electrolyte disorders， acidosis， anemia， lowering blood pressure， anticoagulation and regulating lipid. On the basis of this， the treatment group was also given Qigui Yishen decoction orally. Objective indexes including kidney function，serum plasminogen activator inhibitor-1 (PAI-1)， transforming growth factor-β1 (TGF-β1) and changes of TCM syndrome scores were observed after four weeks. RESULTS After the treatment， TCM syndrome scores in two groups decreased significantly compared with pre-treatment (P<0.05~0.01)， and the treatment group was better than the control group (P<0.05~0.01). PAI-1， ALB/Cr， TGF-β1 and IL-6 levels in two groups decreased significantly after treatment (P<0.05~0.01)， and the treatment group was better the control group (P<0.05~0.01). CONCLUSIONS Qigui Yishen decoction has definite curative effect on protecting renal function and alleviating the progress of renal fibrosis， and its therapeutic effect may be related to the improvement of blood coagulation and endothelial cell function.

Clinical Study of Thunder Fire Moxibustion Combined Celecoxib for Treatment of Knee Osteoarthritis

ZHANGYi-han, XUDao-ming, XUELiang, LIHan-jun, PANGGen-sheng, WANGHe-sheng, ZHANGCong, QINShan

2017, 33(6): 574-578.

[Abstract](1105) [PDF 1035KB](960)

Abstract:
OBJECTIVE To observe the clinical efficacy difference of the thunder-fire moxibustion combined with celecoxib for knee osteoarthritis (KOA) before and after the treatment. METHODS Forty five patients with KOA were randomly divided into the celecoxib-intervened first group and the thunder-fire moxibustion-intervened first group. The celecoxib-intervened first group was treated by celecoxib for two weeks， then treated by thunder-fire moxibustion for another two weeks after one week of elusion， and the thunder-fire moxibustion-intervened first group was treated conversely. Four weeks' follow-up was performed for both groups after the treatment. The degree of tenderness， swelling， activity and WOMAC function score of knee joints were observed before and after the treatment. RESULTS After two weeks of treatment， the degree of tenderness， swelling and activity of knee joint in two groups improved significantly (P<0.05)， and WOMAC function total score also decreased (P<0.05). The degree of tenderness and swelling， activity improvement of the knee joints in the thunder-fire moxibustion-intervened first group were better than those of the celecoxib-intervened first group (P<0.05)， and WOMAC total score decreased more significantly than that of the celecoxib-intervened first group (P<0.05). After one week of elution， there was no significant improvements of the four aspects in the celecoxib-intervened first group， while the improvements of the above four aspects in the thunder-fire moxibustion-intervened first group were significantly different compared with pre-treatment (P<0.05). During the follow-up period of the end of the treatment the degree of tenderness， swelling and activity of knee joints in both groups were significantly improved than those of the pre-treatment (P<0.05)， and WOMAC total score decreased significantly than that of the pre-treatment (P<0.05). But there was no significant difference between two groups. CONCLUSION Thunder-fire moxibustion and celecoxib can improve the joint tenderness， swelling， activity and dysfunction of KOA patients， but the thunder-fire moxibustion shows more advantages in improving KOA patients' joint tenderness， swelling， activity and other functions. The combination of thunder-fire moxibustion and celecoxib shows similar effects no matter which one is used first.

Clinical Study on Buyang Huanwu Decoction in the Treatment of Coronary Heart Disease Angina Pectoris of Qi Deficiency and Blood Stasis Type

WANG Qiang, GU Hui-min, ZHU Jian-zhong, XU Hao-jun, XU Yi-hui

2017, 33(6): 579-582.

[Abstract](1228) [PDF 1010KB](1115)

Abstract:
OBJECTIVE To observe the clinical effect of Buyang Huanwu decoction in the treatment of coronary heart disease angina pectoris of qi deficiency and blood stasis type. METHODS Sixty eight patients with coronary heart disease angina pectoris of qi deficiency and blood stasis type treated by Buyang Huanwu decoction were taken as the treatment group， and was compared with the control group (62 cases treated with conventional western medicine). Angina pectoris efficacy， ECG efficacy， angina pectoris symptoms and blood lipid indexes in two groups were compared. RESULTS The total effective rate of chest tightness and breath shortness improvements in the treatment group was 95.6%， which was significantly higher than that in the control group (80.6%，P<0.05). The total effective rate of palpitation improvement in the treatment group was 88.2%， which was significantly higher than that in the control group (77.4%， P<0.05). The total effective rate of angina pectoris in treatment group and control group was 82.35% and 70.97% respectively， and the total effective rate of ECG was 76.5% and 58.06% respectively， the differences between two groups were significant， P<0.05. The angina pectoris attack frequency and duration in the treatment group decreased more significantly in the treatment group compared with the control group (P<0.05). The nitroglycerin reducing and stopping rate in the treatment group was 80.8%， which was significantly higher than that in the control group (51.6%)， P<0.01.The levels of TC， TG， LDL-C， HDL-C， ApoA1， ApoB and LP (a) in the treatment group were statistically significant after treatment compared with the control group (P<0.01).CONCLUSIONS Buyang Huanwu decoction has definite curative effect for treating coronary heart disease angina pectoris of qi deficiency and blood stasis type， can relieve angina pectoris symptoms and improve blood lipid indexes.

Clinical Study on the Treatment of Persistent Allergic Rhinitis by Scraping Therapy Along Meridian Combined with Loratadine

WANG Hui-min, HUANG Mei, YAN Dao-nan, WU Ji-yong

2017, 33(6): 583-586.

[Abstract](984) [PDF 1007KB](1048)

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OBJECTIVE To study the clinical effect of scraping therapy along meridian combined with loratadine in the treatment of persistent allergic rhinitis (PAR) caused by heat accumulation in lung meridian. METHODS Sixty patients with PAR caused by heat accumulation in lung meridian included in the affiliated hospital of Nanjing University of Chinese Medicine from March， 2015 to September， 2016 were selected and randomly divided into the loratadine control group， the scraping and loratadine combination group， 30 cases in each group. Patients in both groups were treated for 4 weeks. Clinical curative effect was evaluated by total nasal symptoms scores (TNSS)， visual analog scale (VAS)， RQLQ score， total IgE， IL-4 level and total effective rate. RESULTS TNNS， VAS， RQLQ score， total IgE and IL-4 level all decreased in two groups after 4 weeks' treatment (P<0.01). The treatment group was better than the control group in improving VAS， RQLQ score and IL-4 level (P<0.01). And the treatment group was better than the control group in clinical effect and long-term follow-up effect (P<0.05~0.01). CONCLUSIONS Scraping and loratadine combination therapy is an effective treatment for allergic rhinitis and has satisfied long-term effect.

Puerarin Ameliorates Hyperglycemia in HFD Mice Dependent on Activation of GLP-1R Signaling Pathway

WANGChun-jun, JULin-jie, WENXiao-hua, WANGJia-li, SHULuan

2017, 33(6): 587-590.

[Abstract](1019) [PDF 1266KB](1000)

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OBJECTIVE To investigate the effect of puerarin on hyperglycemia in HFD mice and the role of GLP-1R signaling in modulating the hypoglycemic effect of puerarin. METHODS HFD induced diabetic mice was used as the subject， GLP-1 receptor antagonist Exendin 9-39 （Ex 9-39） was used to block the activation of GLP-1R signaling， and in vivo experiments were performed to further evaluate the role of GLP-1R signaling in the hypoglycemic effect of puerarin. RESULTS The glucose homeostasis and the OGTT (oral glucose tolerance tests) of HFD mice were both improved by puerarin， while the effect was impaired by Ex 9-39 significantly. Moreover， puerarin increased serum insulin contents in HFD mice， but such effect was blocked by Ex 9-39 as well. Elevated expression of ki67 in β-cells was observed in pancreatic sections from HFD mice pre-treated with puerarin using Ki-67/insulin immunostaining， suggesting that puerarin could promote β-cell proliferation. The puerarin effect was inhibited when the GLP-1R signaling activation was blocked by Ex9-39. CONCLUSION The results confirmed that the activation of GLP-1R signaling was critical for the anti-diabetic effects of puerarin on HFD induced diabetic mice. Our finding highlights the potential value of Radix puerariae as a dietary supplement for diabetes care.

Urine Metabonomic Study on Xiaofeng Xuanqiao Decoction in Treatment of Anaphylactic Rhinitis Based on UPLC/LTQ-Orbitrap-MS

JIANG Ming-cheng, WANG Shou-chuan, SHAN Jin-jun, XIE Tong, TAO Jia-lei, WANG Xiao-rong

2017, 33(6): 591-596.

[Abstract](1094) [PDF 1411KB](997)

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OBJECTIVE To observe the regulation of Xiaofeng Xuanqiao Decoction(XFTQ) on abnormal metabolites by analyzing the changes of endogenous metabolites in urine of anaphylactic rhinitis(AR) mice， and to explore the mechanism of Xiaofeng Xuanqiao Decoction on treating AR from the perspective of metabonomics. METHODS 60 BALB/c mice were divided into normal group， model group， montelukas group and XFTQ group. Intraperitoneal injection of OVA and local nasal mucosa stimulation was used to establish AR model. Montelukast group and XFTQ group were given montelukast sodium tablets and Xiaofeng Xuanqiao decoction， respectively， for 7d. Urine samples were collected from each group on the last day of administration. UPLC/LTQ-Orbitrap-MS was applied to analyze the metabolic profile changes of each group and the intervention mechanism of drugs. RESULTS AR disordered the metabolism of acid， amine and others in mice. Xiaofeng Decoction mainly regulated the levels of 14 kinds of abnormal metabolites， and it showed good intervention effect on AR mice. CONCLUSION Small molecule metabolites in AR mice are deviated from the normal group. Xiaofeng Xuanqiao Decoction improves the histidine metabolism and cysteine and methionine metabolism to achieve therapeutic effect， mainly through the up-regulation of asymmetric dimethyl-arginine（ADMA）， N-methylnicotinic acid， S-adenosyl-L-methionine(SAMe)， indole-2-carboxylic acid(I2CA)， 6-aminocaproic acid(EACA)， imidazole-4-acetic acid，1-methyl-4-pyridone-5-carboxamide(M4PY)， and the down-regulation of 1-methylhistamine.

Study on Association Between Skin Cell Toxicity of 33 Pungent Essential Oils and Drug Property Characteristics of Chinese Materia Medica

YANGWen-guo, YAOJun-hong, CHENJun, JIANGQiu-dong, YAOYing-zhi, DUANJin-ao

2017, 33(6): 597-602.

[Abstract](1143) [PDF 1285KB](1367)

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OBJECTIVE To analyze the preliminary regulation between the skin cell toxicity of EOs and drug property characteristics of Chinese materia medica(CMM) on the same platform. METHODS The physicochemical parameters of 33 kinds of EOs were measured. MTT assays were applied to evaluate the cell toxicity of EOs on human HaCaT keratinocytes. Finally， data mining technique was applied to analyse experimental data for the purpose to illustrate the relationship between skin cell toxicity of EOs and drug properties of CMMs. RESULTS By non-linear canonical correlation analysis， high correlation（P=0.0411<0.05） was found between skin cell toxicity of EOs and channel tropism property of CMMs. EOs with the properties of liver， lung， stomach， kidney and large intestine channel possess lower skin cell toxicity. However， EOs with the properties of heart， pericardium， spleen， bladder， san-jiao and gall bladder channel possess higher skin cell toxicity. A mathematical model between drug property including four mature， five flavor， channel tropism of CMMs and skin cell toxicity of EOs was established based on non-linear canonical correlation analysis. In the model， significant correlation was found（P=0.0414<0.05）. EOs from CMMs with the properties of cool nature， bitter flavor plus liver or lung or kidney or large intestine channel might be superior in safety towards the skin cells. On contrary， EOs from CMMs with the properties of hot nature， sweet flavor plus heart or pericardium or spleen or bladder or san-jiao or gall bladder channel might be superior in toxicity towards the skin cells. CONCLUSION The skin cell toxicity of EOs is affected by drug property characteristics of CMMs， especially channel tropism property.

Sulfotanshinone ⅡA Sodium Ameliorates Lipopolysaccharide-Peritoneal Dialysis Solution-Elicited Human Peritoneal Mesothelial Cells Injury

ZHOUYao, GAOKun, XIAPing, LIWei, ZHOUZhan-wei, WANGMin-jie, SUNWei, WANGLi-jun, HEWei-ming

2017, 33(6): 603-607.

[Abstract](987) [PDF 1328KB](968)

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OBJECTIVE To study the effect and mechanism of sulfotanshinone ⅡA sodium (STS) in human peritoneal mesothelial cells (HPMCs) injury and peritoneal fibrosis caused by lipopolysaccharide-peritoneal dialysis solution (LPS-PDS). METHODS HPMCs was primary cultured from patients undergoing peritoneal surgery. The cells were incubated with LPS-PDS to mimic the peritoneal dialysis conditions in vitro. After treatment of cells with STS， cellular viability was tested and mRNA were collected and subjected to RT-PCR to evaluated the level of TGF-β1， TIMP-1 and MMP-9. RESULTS Incubation HPMCs with LPS-PDS elicited cell injury. STS attenuated LPS-PDS-induced cell injury by improvement of cellular viability. Furthermore， STS inhibited HPMCs fibrosis as evidenced by suppression of TGF-β1 and TIMP1 induced by LPS-PDS and increasing MMP-9 mRNA level. CONCLUSION STS protects HPMCs against LPS-PDS-induced cell injury. Moreover， STS retards HPMCs fibrosis by decreasing TGF-β1 and TIMP1 mRNA level and increasing MMP-9 mRNA.

The Effect of Electroacupuncture on the Expression of VEGF and VEGFR2 in the Uterus of the Rat Model of Ovulation Induction

LIQian, GONGMei-rong, CHENGJie, MUYan-yun, LUOXi, HUAQi-xin, XIAYou-bing

2017, 33(6): 608-612.

[Abstract](988) [PDF 1537KB](1156)

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OBJECTIVE To observe the effect of electroacupuncture on the expression of VEGF and VEGFR2 in the uterus of the rat model of ovulation induction and to investigate the possible mechanism. METHODS Female SD rats aged 10 weeks were divided into control， model and treat groups randomly， 10 in each group. The model group and the treatment group were peritoneal injected with pregnant horse serum gonadotrophin (PMSG) of 20U and injected of the same dose of human chorionic gonadotropin(hCG) after 48h， and then cohabitated with male SD nice until next morning. A week before cohabitation， the treat group received a consecutive electroacupuncture treatment for 7d(dilatational wave of 2/15Hz for 30min and stimulative intensity of 1mA). The animals were sacrificed 3.5d after the cohabitation. The uterine tissue was taken to observe the morphological change， immunehistochemical method was used to inspect the expression of VEGF and VEGFR2， and qPCR technology was used to determine the expression of VEGF and VEGFR， with the calculation by 2 -△△Ct . RESULTS Macroscopic observation: the surface in control group was regular and smooth; the model group was intumescentia， dull-red and hyperaemia; the treatment group was slightly swollen， rosy and no congestion. The expression of VEGF and VEGFR2 were observed in the 3 groups by immunohistochemical method， with no significant difference in average optical density， but differences in the expression distribution. Compared with the control group， the expression distribution of stromal cells and glands in the model group was more extensive， and the expression of VEGFR2 in myometrium increased significantly(P<0.05). The electroacupuncture treatment inhibited the the expression of VEGFR2 significantly(P<0.05). The expression of VEGF mRNA in uterine tissues of model group was 0.68 times of control group， with significant declining trend; the treat group was 0.88 times of model group， with significant increasing trend. The expression of VEGF2 mRNA in uterine tissues of model group was 0.46 times of control group， with significant declining trend; the treat group was 0.82 times of model group， with significant increasing trend. CONCLUSION The electroacupuncture can adjust the expression of VEGF and its receptor in the uterine tissue of ovulation induction rat model， thus regulate the synchronization of the endometrial planting window caused by hormone.

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

GEYong-liang, TAOJin-hua, WUMu-jun

2017, 33(6): 613-617.

[Abstract](1082) [PDF 1413KB](1006)

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OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice. METHODS 50 ICR mice were divided into five groups randomly， namely， control group， X-ray group， X-ray+dexamethasone group (5mg/kg)， X-ray+baicalin group (20， 40 mg/kg)， with 8 in each group. Pathological changes in the lung tissues were observed. The ratio of lung wet/dry weight was also recorded. Besides， the level of neutrophil in bronchoalveolar lavage fluid (BALF) was detected to evaluate inflammatory cell infiltration. Moreover， the expressions of MDA， IL-6， TNF-α， p-NFκBp65 and p-IκBα， as well as the activity of SOD， were examined in lung. RESULTS Baicalin (20， 40 mg/kg) could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF. The ratio of lung wet/dry weight was also decreased with the treatment of baicalin (20， 40 mg/kg). In addition， the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile. The expressions of p-NFκBp65 and p-IκBα was extensively down-regulated with baicalin (20， 40 mg/kg) preconditioning， taking the expressions of NFκBp65 and IκBα as inner controls. CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.

The Protective Effect of Betulinicd Acid Alleviates CCl₄-Induced Acute Liver Injury in Mice and Its Related Mechanism

XULi-xin, YANLi-jun

2017, 33(6): 618-622.

[Abstract](1088) [PDF 1376KB](1124)

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OBJECTIVE To observe the effect of betulinic acid on CCl₄induced liver injury in mice and its possible molecular mechanisms. METHODS Male ICR mice were randomly divided into 5 groups: normal control group， CCl₄group (20mg/kg)， CCl₄+Silibinin group (200 mg/kg)， CCl₄+betulinic acid group (20 mg/kg)， CCl₄+betulinic acid group (40mg/kg). Mice in the normal control group and CCl₄group were given distilled water while other groups were given drugs in 7 days by gavage. 2h after the last administration， 0.1% CCl₄ with vegetable oil solution (10 mL/kg) by intraperitoneal injection， and the mice in normal control group were given the same volume of vegetable oil solution. 18 h after the model establishment， the blood and liver were collected. Hematoxylin (HE) staining was used to observe the changes of hepatic histopathology. Superoxide dismutase (SOD)， malonaldehyde (MDA)， interleukin-6 (IL-6)， interleukin-1β (IL-1β) and tumor necrosis factor (TNF-α) were measured by corresponding kits. Western blot was used to demonstrate the expression levels of related protein. RESULTS Betulinic acid significantly reduced levels of pro-inflammatory cytokines including interleukin-6 (IL-6)， interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum. Besides， it also attenuated superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. In addition， betulinic acid regulated proteins levels of Nrf-2/HO-1/NF-κB pathway in liver. CONCLUSION Betulinic acid exhibited therapeutical effect on CCl₄induced liver injury in mice which might be related to the regulation of Nrf-2/HO-1/NF-κB pathway.

The Effects of Qingre Huoxue Yangyin Formula on Blood Lipid， Arterial Plaque and Th1/Th2 Imbalance in Atherosclerosis Model Rats

HEJing-jing, MAYun-jing, QIURun-ze, LONGJun, YANShi-hai, YUANDong-ping, LIUFu-ming

2017, 33(6): 623-626.

[Abstract](938) [PDF 1033KB](1007)

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OBJECTIVE To explore the mechanism of intervention with atherosclerosis (AS) by Qingre Huoxue Yangyin Decoction and its functional units. METHODS The AS model of SD rats was successfully established with high fat diet and balloon injury.The model rats were randomly divided into model group， whole group， Huoxue group， Yangyin group， Qingre group and positive group， and received the intragastric administration， respectively.The blank group was fed with normal diet without medication.The changes of each group on blood lipid， arterial lesion and Th1/Th2-related cytokines were observed after 4 weeks. RESULTS ①Compared to the model group， the whole decoction significantly reduced the plaque proportion and TG(P<0.05)， increased HDL-C(P<0.05) ; reduced Th1-related cytokines TNF-α and INF-γ(P<0.05)， increased Th2-related cytokine IL-4(P<0.05).②Each function unit compared with the model group: Yangyin group obviously elevated IL-4 and decreased TG (P<0.05)， Qingre group obviously decreased the IFN-γ， LDL-C and plaque proportion (P<0.05)，Huoxue group significantly reduced plaque ratio（P<0.01）. CONCLUSION Qingre Yangyin Huoxue Decoction and each function unit can play a role in anti-atherogenesis by lowering blood lipids and regulating Th1 and Th2 subpopulations differentiation imbalance.

Gancao Xiexin Decoction Ameliorates TNBS/Ethanol-Induced Ulcerative Colitis in SD Rats Through Regulation of the IL-6/STAT3 Signaling Pathway

CHENHao, XUSu, YANShuai, CHENBing-xue, ZHANGBo, LUQin, SUNHui, ZHOUXing-xin, ZENGLi

2017, 33(6): 627-632.

[Abstract](1099) [PDF 1680KB](1115)

Abstract:
OBJECTIVE To observe the curative effect of Gancao Xiexin Decoction(GCXXT) on ulcerative colitis (UC) model rats and explore the regulation mechanism of related molecules in the pathways of IL-6/STAT3. METHODS 60 SD rats were randomly divided into 6 groups: control， TNBS/ethanol， mesalazine and GCXXT (2.93， 5.86， 11.72g/kg) groups. Blood serum was extracted to detect the content of ALT， AST， BUN and Cr. Colon tissues were observed by optical microscope. The expressions of protein of IL-6， STAT3 and phosphate STAT3 were evaluated by immunohistochemistry. The expressions of mRNA of IL-6 and STAT3 were analyzed by qPCR. RESULTS Compared with the blank group， AST in serum of model group increased obviously (P<0.01). However， ALT， BUN and Cr displayed no change (P>0.05). Pathological scores of colon tissues of model group increased significantly (P<0.01). The expressions of protein and mRNA of IL-6 and STAT3 in colon tissues increased in model group (P<0.01). Furthermore， the activation of STAT3 increased (P<0.01). Compared with the model group， AST in serum of rats of three groups treated with GCXXT decreased (P<0.01). Pathological scores of colon tissues of mesalazine group and medium dose GCXXT group decreased significantly (P<0.01). After the treatment of mesalazine or GCXXT， the expression of protein of IL-6， STAT3 and phosphate STAT3 reduced (P<0.01). The expression of mRNA of IL-6 and STAT3 decreased (P<0.01). CONCLUSION GCXXT can alleviate the pathological changes of colon， and medium dose of it can lead to satisfactory results. The mechanism may be related to its abilities to decrease expression of IL-6 in colonic tissue， inhibit IL-6/STAT3 signaling pathway and reduce the expression and activation of STAT3. These results showed that GCXXT is effective and safe in ameliorating ulcerative colitis.

UPLC Fingerprint and Chemical Pattern Recognition Method of Scutellaria Baicalensis Stem-Leaf from Different Regions

YAN Bao-fei, ZHU Shao-qing, SU Shu-lan, ZHU Zhen-hua, GUO Sheng, ZENG Hui-ting, QIAN Da-wei, DUAN Jin-ao

2017, 33(6): 633-638.

[Abstract](971) [PDF 1180KB](1103)

Abstract:
OBJECTIVE To study and establish the UPLC fingerprint of Scutellaria baicalensis stem-leaf(SBSL) and rapidly identify the specific peaks by UPLC-QTOF/MS， and combined with the study of chemical pattern recognition to provide scientific basis for resources circulating utilization of the SBSL. METHODS The fingerprint of SBSL was established by UPLC， the samples were conducted by ACQUITY UPLC BEH C₁₈ (100mm×2.1mm， 1.7μm) and eluted with acetonitrile and 0.1% formic acid with the flow rate of 0.4mL/min. The detection wavelength was set at 254nm and column temperature was 35℃. Negative ion mode was chosen for qualitative analysis. The capillary voltage was set at 3.0kV. The nebulization gas was set to 800L/h at 400℃， and the source temperature was 120℃. The similarity evaluation， cluster analysis (CA)， and principal component analysis (PCA) were used to deal with the experimental data， in order to find out the similarities and differences among the 11 batches of SBSL from 10 different regions. RESULTS The specific chromatogram of SBSL was obtained， and 19 common peaks were identified by ESI-QTOF/MS. Similarities of the 11 batches of samples from 10 regions were over 0.9， the results of CA and PCA were consistent with similarity evaluation. CONCLUSION The method established in this paper is rapid， accurate， reliable and reproducible. The establishment of UPLC fingerprint of SBSL and the application of chemical pattern recognition can provide a more comprehensive reference for the quality control of SBSL and the resources circulating utilization value of non-medicinal parts of S. baicalensis.

Study on TCM Physical Personality and Constitution Based on Diabetes Mellitus Complicated with Coronary Heart Disease Risks Predicted by Decision Tree Model

LYUHang, YANGQiu-li, DUJian, WANGHao, LIUYuan, ZHAOZhi-qiang

2017, 33(6): 639-642.

[Abstract](1029) [PDF 1129KB](1041)

Abstract:
OBJECTIVE To study the prediction effect of TCM physical personality and constitution on the risks of MD complicated with CHD. METHODS TCM physical personality and constitution of 110 T2MD patients were tested by five pattern personality inventor and five-five constitution. CHAID model of decision tree in EXCEL and SPSS was used to establish disease risk model of MD complicated with CHD predicted by TCM personality and constitution types. And the prediction accuracy of the model was evaluated. RESULTS Tested by cross validation， the prediction accuracy of MD disease model established with the Shao-yin personality， Yin-cold constitution and blood-stasis constitution as the predictor variables was relatively higher (93.6%)， and three rules for predicting CHD risk were obtained. CONCLUSIONS The prediction rules of the MD complicated with CHD by TCM personality and constitution are consistent with the traditional understanding of Chinese medicine， which provides a reference for high-risk population's early screening， CHD prevention and treatment， clinical differentiation and treatment.

Study on the Relationship Between TCM Constitution and Sleeping Habit in Healthy Elderly People

WANGJue, LIGuo-chun, HEWen-bin

2017, 33(6): 643-645.

[Abstract](1007) [PDF 983KB](1032)

Abstract:
OBJECTIVE To study the relationship between TCM constitution and sleeping habit in healthy elderly people. METHODS Classification and diagnosis table of traditional Chinese medicine constitution was adopted to investigate and analyze the TCM constitution of the healthy elderly people who are over 80 year-old in Rugao， Liyang and Taicang. Logistic regression analysis was used to study the relationship between TCM constitution and sleeping habit in healthy elderly people. RESULTS Getting up late was positively related with qi deficiency constitution， yin deficiency constitution， qi stagnation constitution and blood stasis constitution. Sleep late was positively related with qi stagnation constitution. Getting up early was negatively correlated with qi deficiency constitution， yang deficiency constitution， yin deficiency constitution， phlegm dampness constitution and blood stasis constitution. Sleep early was negatively correlated with yang deficiency constitution， yin deficiency constitution， phlegm dampness constitution and blood stasis constitution. Sleep disorder was positively related with yang deficiency constitution and qi stagnation constitution. CONCLUSIONS Comparing with sleep late， getting up late causes more biased constitution and greater damage to health， which embodies TCM thought that paying attention to yang qi and taking yang as the root.

Comparison of Clinical Medication Between Premature Menstruation and Delayed Menstruation

SHENXiao-jing, CHENXu, SHEKan-kan

2017, 33(6): 646-648.

[Abstract](903) [PDF 983KB](1002)

Abstract:
OBJECTIVE To find out herbs with significant differences in using frequency of prescriptions in the treatment of premature menstruation and delayed menstruation， and to determine their differences. METHODS Sixty five premature menstruation cases and 367 delayed menstruation were selected. Chi-square test and logistic regression were used to analyze the differences in herbs use between premature menstruation and delayed menstruation prescriptions. RESULTS There were 7 kinds of used herbs were significantly different between premature menstruation and delayed menstruation prescriptions， and they were jin yin hua (Flos Lonicerae Japonicae)， huang bai (Cortex Phellodendri Chinensis)， pu gong ying (Herba Taraxaci)， han lian cao (Herba Ecliptae)， ou jie (Nodus Nelumbinis Rhizomatis)， chang pu (Rhizoma Acori Graminei) and dang gui (Radix Angelicae Sinensis). In addition to dang gui (Radix Angelicae Sinensis)， the rest herbs were higher frequently used in premature menstruation than that in the delayed menstruation， from high to low according to the difference， they were han lian cao (Herba Ecliptae)， ou jie (Nodus Nelumbinis Rhizomatis)， pu gong ying (Herba Taraxaci)， chang pu (Rhizoma Acori Graminei)， jin yin hua (Flos Lonicerae Japonicae) and huang bai (Cortex Phellodendri Chinensis). CONCLUSIONS The herbs use difference between premature menstruation and delayed menstruation could be determined through chi-square test. Then， logistic regression can be used to analyze the difference degree， which can make the difference more specific， and provide reference for clinical herb use for premature menstruation and delayed menstruation.

2017 Vol. 33, No. 6

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