南京中医药大学学报

Origin and Academic Values of Theory of Thirteen Pathogenesis

ZHOU Zhong-ying, YE Fang, ZHOU Xue-ping, GUO Li-zhong, WU Mian-hua, CHENG Hai-bo, FANG Liang, LI Liu, FENG Zhe

2017, 33(5): 433-435.

[Abstract](1359) [PDF 336KB](1561)

Abstract:
By reviewing the original intention of constructing the syndrome differentiation system on the basis of thirteen pathogenesis， to emphasize that the pathogenesis is the key point for syndrome differentiation and treatment， which is the core of all TCM theories. Thirteen pathogenesis is established on the basis of nineteen pathogenesis in The Yellow Emperor's Inner Classic， and integrated the theory of important pathogenesis of later generations physicians. This paper puts forward a new syndrome differentiation system based on thirteen pathogenesis， which integrates the existing traditional dialectical methods， enriches and develops the theory system of pathogenesis of TCM.

Understanding and Innovation of New Theory of TCM Syndrome Differentiation Based on Holism

YE Fang, ZHOU Xue-ping, CHENG Hai-bo, WU Mian-hua, ZHOU Zhong-ying

2017, 33(5): 436-439.

[Abstract](1401) [PDF 361KB](1342)

Abstract:
It is believed that theory of qi-monism is the core of TCM theories， which guides the syndrome differentiation and treatment of TCM. The different differentiations methods of TCM， such as syndrome differentiation in accordance with eight principles， syndrome differentiation in accordance with five-zang organs， syndrome differentiation in accordance with qi， blood and body fluid and syndrome differentiation in accordance with six meridians， are all the methods used to understanding the pathogenesis of human body's yin-yang balance. In fact， TCM pathogenesis refers to understand the mechanism of health， occurrence and development of disease from the perspective and thinking of Chinese medicine. While， theory of qi-monism combined with theories of yin and yang， visceral manifestations， qi， blood and body fluid， six meridians， constitutes the epistemological basis of the holism of TCM.

Exploration of Several Issues about TCM Pathogenesis Differentiation System

FANGLiang, YEFang, ZHOUXue-ping, ZHOUZhong-ying

2017, 33(5): 440-442.

[Abstract](1368) [PDF 343KB](1224)

Abstract:
This paper expounds the construction process， innovation and significance of Professor Zhou Zhong-ying's pathogenesis differentiation system， and discusses the concept of pathogenesis and its relationship with syndrome. It is believed that the highest realm of TCM syndrome differentiation is applying the theory flexibly， and understanding the pathogenesis is the understanding of the essence of the disease. Pathogenesis is the definition of the nature of disease， while syndrome is the summarization of pathogenesis. Both of them are characterized by variability and regularity. The pathogenesis differentiation system not only embodies the clinical characteristics of pathogenesis changing in accordance with syndrome， but also presents the flexibility of syndrome differentiation. It also puts forward that future pathogenesis research can be designed from different aspects and levels， in order to guide clinical practice.

Exploration of the Common Processing Mechanism and Characteristic Quality Standard Based on the Theory of Seed Drugs Be Stir-Heated

CAI Bao-chang, 2, 3*

2017, 33(5): 443-447.

[Abstract](1816) [PDF 417KB](1342)

Abstract:
The processing theory of seed drugs be stir-heated before using for the seed medicine has a rich scientific connotation. However， the internal chemical changes and changing laws are not clear， the effective substances are not definite， and characteristic quality standards are in need， so it is difficult to control the quality of the seed drugs effectively. When processing the Chinese herbal medicines， the same technical methods may cause some common reactions frequently. Revealing the common mechanism of seed drugs be stir-heated before using in this further study has guiding meaning to establish characteristic quality standards. On the basic of the theory of seed drugs be stir-heated， this study will use the spectrum-activity relationship method to study the changes of the chemical composition and pharmacological effect， clarify the effective basis， and reveal common mechanism during the stir-frying process. Meanwhile， this paper will use pharmacokinetics method to clarify the effective substances in vivo of Chinese medicine. On this basis， multi-components by one marker， LC-MS and other methods will be employed to establish characteristic standards for these seed drugs， and then to guide the standardized production and clinical use of seed drugs.

The Detoxification Technology and Common Detoxification Mechanism of Toxic Chinese Medicine

WANGWei, WANGKui-long, SHANXue-lian, CHENGZi-ye, CHENYun, YUHong-li, WUHao

2017, 33(5): 448-452.

[Abstract](1324) [PDF 1209KB](1913)

Abstract:
Chinese medicine processing is a kind of traditional pharmaceutical technology， which can reduce toxicity， enhance the curative effect， change the properties and facilitate dispensing preparations in clinical use. The technology of detoxification is a great pioneering work for the safe application of toxic Chinese medicine， which can reduce the side effects of certain drugs， ensure the clinical safe use and expand the scope of clinical application through processing. In this paper， we searched the toxic Chinese medicine and the processing technology in ancient literatures of TCM， and then we summarized and analyzed their latest studies at home and abroad. Thus， we make a summary of toxic Chinese medicine including its classification， site of action， the toxic components， detoxification and its clinical application. Meanwhile， we proposed an opinion that toxic Chinese medicines with the same processing technology had common detoxification rules. Therefore， we hope this work will provide new perspectives for the research on the processing of toxic Chinese medicine and the mechanism of detoxification.

Study on Fingerprints of Traditional Chinese Medicine Processing Accessories of Rice Vinegar

LU Jin-lan, ZHU Xing-yu, LIU Ling, ZHANG Xi-qiong, XU Liu, CHEN Zhi-peng, LI Wei-dong

2017, 33(5): 463-469.

[Abstract](1290) [PDF 925KB](1165)

Abstract:
OBJECTIVE To provide the basis for the medicinal quality standard of Chinese medicine processing accessories of rice vinegar through analysis with fingerprints of different kinds of origin and brand of rice vinegar. METHODS 33 batches of vinegar were collected. The mobile phase， column， elution， the mobile phase pH were investigated and the analysis method of HPLC fingerprint was optimazated， Among them， 20 batch of rice vinegar fingerprint were chosen to perform the similarity， cluster analysis and principal component analysis. RESULTS The concentration of rice vinegar was diluted at 1∶3 when it was injected. Chromatographic column was Thermo Hypersil GOLD （5 μm，250 mm×4.6 mm）; the mobile phase was composed of methanol and 0.07% phosphoric acid aqueous solution and a gradient elution program at flow rate of 1 mL/min was applied. The column temperature maintained at 30 ℃. The injection volume was 10 μL. Then， the separation of characteristic peaks was optimum. Rice vinegar was divided into black vinegar and white vinegar. Furthermore， there was a big difference between black vinegar and white vinegar. The white vinegar almost showed only one peak of acetic acid， so， it's impossible to distinguish between brewing white vinegar and formulated white vinegar by HPLC. Similarity evaluation showed that 20 batch of white vinegar's fingerprints had palpable difference. Besides， clustering analysis and principal component analysis splited them into several categories. Different types of Hengshun vinegar indicated high similarity. Hence it could be seen that the quality of rice vinegar from the same brand was uniform and stable because of similar brewing processes and similar materials . CONCLUSION There are varieties of rice vinegar on the market with extensive source and uneven quality. 20 batches of black rice vinegar were chosen to compare the differences by HPLC fingerprints analysis， which provides reference for the medicinal quality control standard of traditional Chinese medicine processing accessories of rice vinegar.

Determination of the Related Components in Prepared Rhubarb Decoction and Its Pharmacodynamics Study

ZHUHui, LIUXiao, ZHUTing-ting, WANGXiao-li, ZHUXiao-chai, CAIBao-chang

2017, 33(5): 470-475.

[Abstract](1368) [PDF 657KB](1190)

Abstract:
OBJECTIVE To develop an HPLC method for determination of main components in prepared rhubarb decoction and investigate its effects on rats with noxious heat blood stasis syndrome. METHODS The analysis was performed on a Hypersil GOLD C₁₈ column with the mobile phase of 0.1% phosphoric acid solution(A)-acetonitrile(B). The detection wavelength was set at 254 nm and the column temperature was 30 ℃. The method was validated and the samples were determined under this condition. The abdominal aortic blood of rats with noxious heat blood stasis syndrome was collected and tested to obtain the hemorheological parameters. Also， the TXB₂ and 6-keto-PGF_1α levels in rats plasma were measured. RESULTS The developed HPLC method showed good linearity within certain concentration ranges. And the precision， repeatability， stability and recovery all met the requirements. Compared with rats in model group， the whole blood viscosity at four shear rates（P<0.05）， plasma viscosity（P<0.01） and the level of plasma fibrinogen content（P<0.01） in the high dose group were significantly decreased after doing， and the TT（P<0.01） and PT（P<0.01） values were significantly prolonged. Furthermore， the levels of TXB₂（decreased， P<0.05） and 6-keto-PGF1α（increased，P<0.05）were significantly changed， and their ratio was corrected to be normal. CONCLUSION The method was simple， accurate and reliable，which could be used for quality control of prepared rhubarb decoction. Prepared rhubarb could improve vascular endothelial function by modifying blood rheology， promoting the secretion of PGI₂， reducing the generation of TXA₂ and recovering the balance of TXB₂/6-keto-PGF₁_α.

Comment on Zhongjing's Formula for Treating Cough due to Abnormal Rise of Qi

LI Fei

2017, 33(5): 476-479.

[Abstract](1445) [PDF 383KB](2539)

Abstract:
ZHANG Zhongjing had divided cough due to abnormal rise of qi into the following types: syndrome of exterior and interior， syndrome of cold and heat， syndrome of deficiency and excess. For internal disease mainly caused by cold fluid， the treatment should warm the lung to reduce watery phlegm， and stop cough to relieve asthma. For prolonged diseased lead to heat syndrome， herbs that clearing heat and purging fire and eliminating toxin should be added on the basis of removing dampness. For external disease pertaining to dual exterior and interior syndrome， the treatment should induce sweating to relieve the exterior. Meanwhile， herbs should be combined according to diseases conditions， such as ventilating the lung to suppress cough herbs， warming the lung to reduce watery phlegm herbs， resolving phlegm herbs， releasing the exterior and harmonizing the nutritive and defensive levels herbs， clearing heat herbs， restraining the lung to suppress cough herbs and purging lung to alleviating water retention herbs. On the basis of Zhong-jing's formula， the later physicians also combined with invigorating qi and nourishing blood herbs， tonifying kidney and suppress asthma herbs.

Clinical Observation on the Effect of Sanhuang Tang in the Treatment of Polycystic Ovary Syndrome of Phlegm Dampness Heat Accumulation Type

YELi-fang, SHAOXin, LIUSu, XUWei-long, RUANYuan, XINGYing, WANGLin-jie, GUChun-yan

2017, 33(5): 480-483.

[Abstract](1443) [PDF 376KB](1253)

Abstract:
OBJECTIVE To observe the clinical effect of Sanhuang Tang in the treatment of polycystic ovary syndrome (PCOS) of phlegm dampness heat accumulation type. METHODS Sixty-five PCOS patients of phlegm dampness heat accumulation type were randomly divided into the Chinese medicine plus group and the western medicine control group. Both group were taking metformin orally， on the basis of this the Chinese medicine group was also taking Sanhuang Tang granules orally. The clinical effect was observed for three months. Clinical symptoms， sex hormone levels， body mass and insulin resistance metabolic indexes were analyzed before and after the treatment. RESULTS The improvements of clinical symptoms， sex hormone levels， body mass and insulin resistance indexes in the Chinese medicine plus group were better than those in the control group (P<0.05～0.01).CONCLUSIONS The clinical effect of Sanhuang Tang plus group is better than that of the western medicine alone control group.

Relationship Between TCM Syndrome Types and Clinical Biochemical Indexes in Type 2 Diabetes Mellitus

ZHANG Wen-jia, FU Xiao-le, XIN Xiao-yi

2017, 33(5): 484-487.

[Abstract](1344) [PDF 367KB](1214)

Abstract:
OBJECTIVE To study the relationship between TCM syndrome types and clinical biochemical indexes in type 2 diabetes mellitus， and to an objective basis for TCM syndrome differentiation of type 2 diabetes mellitus. METHODS 120 type 2 diabetes mellitus patients corresponding to the study criteria were collected. Syndrome differentiation was made for these patients according to yin deficiency and heat excess， spleen deficiency， phlegm accumulation， deficiency of both Qi and Yin and qi deficiency and blood stasis. At the same time， 40 healthy subjects were matched as controls. The relationships between different types and age， body mass index (BMI)， glucose metabolism， lipid metabolism， hypersensitivity C reactive protein (hs-CRP)， insulin resistance indexes (HOMA-IR) and serum insulin (FINS) were analyzed. RESULTS ①Compared with the normal control group， BMI in the yin deficiency and heat excess group decreased significantly (P<0.01)， BMI in the spleen deficiency and phlegm accumulation group increased significantly (P<0.01). ②Compared with the normal control group， all indexes of TC， TG and LDL in the diabetes mellitus group increased (P<0.05)， HDL index decreased (P<0.05). ③All FPG， HOMA-IR， HbA1c and hs-CRP levels in the diabetes mellitus group increased compared with the normal control group (P<0.05～0.01). Apart for the yin deficiency and heat excess group， FINS indexes in the rest three group had significant differences compared with the normal control group (P<0.05). CONCLUSION There are correlations between various TCM syndromes of type 2 diabetes mellitus and lipid metabolism， glucose metabolism， body mass index and insulin resistance index. The study of the relationship between them will contribute to the objectification of TCM syndrome differentiation in diabetes mellitus.

Study on the Action Mechanism of Combined Jiaweishoutai Wan and Progesterone in Regulating NKG2D and NKG2A Expression for Treating URSA of Kidney Deficiency Type

CAOWei-ping, SHIWei, LIXia, MAQing, WUMei-ling, WANGDong-mei

2017, 33(5): 488-492.

[Abstract](1233) [PDF 609KB](1070)

Abstract:
OBJECTIVE To investigate NK cells activity changes in the occurrence of URSA， and explain the action mechanism of combined Gushen Antai experienced formula "Jiaweishoutai Wan" and progesterone in the treatment of URSA by regulating expression changes of NK cell receptor to induce immune tolerance. METHODS Twenty patients with kidney deficiency type URSA successfully treated by combined Jiaweishoutai Wan and progesterone were chosen. And URSA pregnancy loss patients and HEP patients at the same period were selected， 15 cases in each group. The expression of NKG2D and NKG2A in peripheral blood were detected by flow cytometry. The levels of E2， P and β-HCG in the URSA pregnancy group and HEP group were compared after treatment. RESULTS In URSA pregnancy loss group， the expression of NKG2D of NK cells was higher and NKG2A was lower， the differences were statistically different compared with HEP group (P<0.01). NKG2D expression level of pNK cells in URSA pregnancy group was significantly lower than that of pretreatment， and the difference was statistically different compared with URSA pregnancy loss group (P<0.01); and the NKG2A expression level was obviously higher than that of pretreatment (P<0.01)， the difference was not statistically significant compared with HEP group， and the difference was statistically different compared with URSA pregnancy loss group (P<0.01). After treating with combined Jiaweishoutai Wan and progesterone， the blood serum E2， P and β-HCG levels in URSA pregnancy group were all not significantly different with that in the HEP group (P>0.05). CONCLUSION The expression of NK cells receptors including NKG2D and NKG2A is involving in the occurrence of URSA. The combined Jiaweishoutai Wan and progesterone can treat URSA by down regulating the NKG2D expression levels of URSA patients and up regulating NKG2A expression levels to induce maternal fetal immune tolerance. Gushen Antai method is an effective treating method for URSA of kidney deficiency type. Jiaweishoutai Wan is an effective formula for treating URSA of kidney deficiency type.

Therapeutical Effects of Aspirin and XueShuanTong on Patients with the Drug Resistance of ITGB3 Genes Polymorphism

YANG Cheng-zhi, LI Zai-li, MENG Xin-yan

2017, 33(5): 493-496.

[Abstract](1289) [PDF 368KB](1217)

Abstract:
OBJECTIVE To investigate the relationship between ITGB3 gene polymorphism and therapeutic effects of XueShuanTong and aspirin on cerebral infarction with anti-platelet. METHODS 192 patients with cerebral infarction were collected and randomly divided into 4 groups. According to patients general information， biomedical index and thromboelastogram， DNA genome were extracted from venous blood and detected by using Snapshot method， and then analyzed the situations of anti-platelet and compared the results of 4 groups. RESULTS Generally， the inhibitory ratio of platelet increased obviously after the patients were treated with drugs. Compared with aspirin group， the inhibitory ratio of platelet increased obviously in patients with ITGB3 genes(AA， AG， GG) treated by XueShuanTong pharmaceutics， and no significant difference appeared among these 3 groups. CONCLUSION XueShuanTong pharmaceutics can inhibit platelet aggregation， which will prevent the drug resistance in patients with mutant ITGB3 genes(AA and GG).

Clinical Curative Effects Analysis of Self-made Yishen Jianpi Huayu Formula in the Treatment of Syndrome of Spleen and Kidney Yang Deficiency of Postmenopausal Osteoporosis

HUANGLin-ling, WUJuan, DENGYing-hui

2017, 33(5): 497-500.

[Abstract](1251) [PDF 397KB](1130)

Abstract:
OBJECTIVE To study the Clinical curative effect of self-made Yishen Jianpi Huayu Formula in the treatment of syndrome of spleen and kidney yang deficiency of postmenopausal osteoporosis. METHODS From January 2016 to January 2017， 120 postmenopausal osteoporosis patients included in our hospital were selected and randomly divided into the observation group and the control group by random number， 60 cases in each group. Both groups were given calcium carbonate D3 tablets. On the basis of this， the control group was given xianlinggubao capsules， and the observation group was given self-made Yishen Jianpi Huayu Formula. Both groups were treated for three months. RESULTS After treatment， the total clinical symptoms cores of lumbar and back pain， soreness and weakness of waist and knees， increasing of night urine， aversion to cold and cold limbs and lassitude in both groups were decreased significantly(P<0.01)， but the observation group decreased more significantly (P<0.01). After treatment， the total effective rate in the observation group was 93.33%， which was significantly higher than that in the control group (80.00%， P<0.01). The bone mineral density of lumbar spine， L2~L4， femoral neck， greater trochanter of femur and Ward's pyramidal bone were significantly increased compared with pretreatment (P<0.01)， but the observation group increased more significantly (P<0.01). Bone specific alkaline phosphatase (B-ALP) and tartrate resistant acid phosphatase(TRACP) in both group were decreased significantly (P<0.01) compared with pretreatment， but the observation group decreased more significantly (P<0.01). Estradiol (E₂)， insulin growth factor (IGF-1) in both group were increased significantly compared with pretreatment (P<0.01)， and the observation group increased more significantly (P<0.01). The adverse events occurrence rates in both groups had no significant difference (P＞0.05). CONCLUSION Self-made Yishen Jianpi Huayu formula can significantly improve the clinical symptoms of postmenopausal osteoporosis， and improve patients' bone metabolism and bone mineral density at the same time. Its action mechanism may be associated with increased estrogen and IGF-1 levels.

Effect of Siwu Decoction in Treatment of Hidden Hemorrhage after PFNA with Senile Intertrochanteric Fractures

JIAO Feng

2017, 33(5): 501-503.

[Abstract](1250) [PDF 353KB](1126)

Abstract:
OBJECTIVE To observe the effect of Siwu decoction in treatment of hidden hemorrhage after PFNA with senile intertrochanteric fractures， and to provide the reference for TCM on the hidden hemorrhage with senile intertrochanteric fractures patients. METHODS Forty PFNA postoperative patients with senile intertrochanteric fractures patients were divided into the treatment group and the control group according to whether taking the decoction. On the basis of conventional anti-infection symptomatic treatment and dietary guidance， the treatment group was also beginning to take Siwu decoction continuously after three days of surgery， taking for 10 d totally. All patients' height， body mass， intraoperative and postoperative overt blood loss volume were observed and recorded. Hemoglobin (Hb) and hematocrit(HCT) were tested and recorded before and after treatment. Red blood cell volume was calculated by Gross equation to evaluate the hidden hemorrhage volume. RESULTS After the treatment， Hb content in the treatment group increased significantly， hidden hemorrhage volume improved obviously (P<0.01)， which was better than that in the control group (P<0.05). CONCLUSION Siwu decoction can improve the hidden hemorrhage of senile intertrochanteric fractures patients after PFNA.

Characterization of Radix Isatidis Protein and its Ultrasound-assisted Extraction Process Optimization with Response Surface Methodology

XIAO Ping, LI Hua-lu, CHEN Wan-ting, ZHAO Xian-wen, QIU Xian-gui, LI Xiang, CHEN Jian-wei, DUAN Jin-ao

2017, 33(5): 504-508.

[Abstract](1444) [PDF 708KB](1166)

Abstract:
OBJECTIVE To characterize the molecular weight of Radix Isatidis protein (RIP) and optimize the extraction process of protein from Radix Isatidis. METHODS Response surface methodology (RSM) was applied to optimize the extraction of protein from Radix Isatidis. Extraction time， liquid-to-solid ratio and pH value were set as the investigated factors with respect to the protein yield. In addition， Design Expert software was used for data analysis. The RIP was characterized for composition using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Scanning electron microscopy (SEM) analysis was performed to observe microstructure of the Radix Isatidis powder before and after ultrasound-assisted extraction (UAE). RESULTS Based on the RSM analysis， optimum conditions were determined as follows: twice ultrasonic extraction in 50 mmol/L Tris-HCl buffer solution， pH at 7.8， liquid-to-solid ratio at 80∶1 and extraction for 65 min each time. Under the optimized conditions， the experimental values were 0.705%， which is in close agreement with values predicted by the model. The characterization of the RIP demonstrated that it contained five major groups of protein bands， namely bands of 19.2 kDa， 21.5 kDa， 24.8 kDa， 34~43 kDa and >170 kDa respectively. CONCLUSION RSM can be applied for the optimization of extraction process of RIP， which is effective， stable and feasible.

The Effects of Pricking Blood Therapy on Promotor Methylation of IL-1β， IL-10 in Rats Local Ankle Joint with Acute Gouty Arthritis Model

LYUKai-lu, XIAYou-bin, CHENGJie, MUYan-yun, LIANGSha, LUOXi

2017, 33(5): 509-514.

[Abstract](1240) [PDF 653KB](1261)

Abstract:
OBJECTIVE To observe the cross section diameter and the effects of pricking blood therapy on acute gouty arthrits rats model's interleukin-1β (IL-1β)， interleukin -10 (IL-10) mRNA expression and promoter methylation in local ankle， and explore the epigenetics mechanisms of pricking blood therapy's treatment. METHODS 36 SD rats were randomly divided into normal control group， urate arthritis group， ibuprofen group and bloodletting group. The acute gouty arthrits model set up by injecting uric acid into the right ankle joint cavity .The ibuprofen group was treated by gastric perfusion of ibuprofen， while the bloodletting group was pricked at the right Kunlun points. The swelling index of the modeling joint was evaluated， and the morphological changes were observed under light microscope. The mRNA expression levels and promoter methylation status of IL-1β and IL-10 in rats local ankle were detected by qPCR and pyrosequencing detection. RESULTS Compared with the urate arthritis group and ibuprofen group， the joint swelling index in the bloodletting group was decreased (P<0.01) after the treatment. Pricking blood therapy reduced intracellular deposition of uric acid in the joint cavity， and inhibited inflammatory cells to improve the synovial membrane tissue. Compared with urate arthritis group， the IL-1β mRNA expression levels of blood group was decreased significantly (P<0.01). IL-1β promoter methylation and mRNA expression didn't showed a negative correlation. Compared with normal control group， urate arthritis group and ibuprofen group， the expression levels of IL-10 mRNA in blood group increased significantly (P<0.01)， and the average methylation rate of IL-10 among blood group decreased significantly (P<0.05~0.01). IL-10 promoter methylation and mRNA expression showed a strong negative correlation (r=-0.899， P<0.01). CONCLUSION In epigenetics， the methylation of IL-10 can regulate the gene expression， which can be one of important mechanisms which shows anti-inflammatory effects of pricking blood therapy， .

Effect of Xiaoai Jiedu Decoction Recipe on Colon Cancer and Distribution of Treg Cells

TANJia-ni, SHIWen-jing, SHENWei-xing, XUChang-liang, SUNDong-dong, CHENGHai-bo

2017, 33(5): 515-518.

[Abstract](1285) [PDF 690KB](1503)

Abstract:
OBJECTIVE To explore the anti-tumor effect and mechanism of Xiaoai Jiedu Decoction on colon cancer. METHODS CT26 cells were injected subcutaneously into the right armpit of C57 mice to establish the tumor-bearing model. These mouse were sacrificed after 14 d administration. Mononuclear cells of the peripheral blood were isolated and the distribution of Treg cells were detected by flow cytometry. The expression of Foxp3 protein in tumor tissue was detected by immunohistochemistry method. RESULTS Xiaoai Jiedu Decoction significantly inhibited the proliferation of colon cancer in mice， decreased the distribution of CD4⁺Foxp3⁺ Treg cells in peripheral blood and decreased the expression of Foxp3 protein in tumor tissues. CONCLUSION Xiaoai Jiedu Decoction can significantly inhibit the proliferation of colon cancer by decreasing the distribution of Treg cells in peripheral blood and the expression of Foxp3 in the tumor tissues， thus affecting the downstream signal pathway.

Effects of Classical Prescriptions of Blood-activating and Organ-purging Formula on Immunologic Barrier Function of Intestinal Mucosa in Rats with Postoperative Peritoneal Adhesion

YANShuai, YUEYin-zi, YANGLi-li, BIANYao-yao, ZENGLi

2017, 33(5): 519-523.

[Abstract](1283) [PDF 1160KB](1193)

Abstract:
OBJECTIVE To explore the effect of blood-activating and organ-purging formula in vivo of intestinal mucosal barrier after surgery in peritoneal adhesion. METHODS 60 male SD rats were randomly divided into control group， model group and Huoxue Tongfu Decoction group. Besides the control group， the rest of the rats were established with grater method， and the same dose of drugs were given at 3， 6， 12， 24 h after operation. The blood and tissue samples were taken after 48 h. TNF-α levels in plasma were determined by ELISA， sIgA in intestinal mucosa was detected by immunohistochemical method and the number of content of CD4 cells， lymphocytes apoptosis of intestinal mucosa were detected by TUNEL. RESULTS Compared with the control group， the plasma levels of TNF-α in the model group of 6， 12， 24 h showed significant difference (P<0.05~0.01)， intestinal mucosa under lymphocyte were apoptosis significantly (P<0.01)， 12 h and 24 h secretion IgA significantly increased (P<0.05)， and the model group 12 h， 24 h and 3 h， 6 h， submucous lymphocyte apoptosis decreased close to the level of control group， model group 3 h CD4 in intestinal mucosa cell number decreased significantly (P<0.01)， there was no significant difference in the number of CD4 cells when three groups at 6 h， 12 h and 24 h (P>0.05). Prescription in 3 h after operation can significantly reduce the levels of TNF-α in plasma of model rats increased number of intestinal mucosae， the content of IgA and CD4 cells decreased， submucous lymphocyte apoptosis. CONCLUSION Blood-activating and organ-purging formula group can reduce the blood level of TNF- alpha and submucous lymphocyte apoptosis， increase the number of intestinal mucosal s content of IgA and CD4 cells， improving local immunity， inhibiting the inflammatory reaction.

Effects of Experimental Studies Yunpi Rougan Fang on 5-HT， VIP and SP in Constipation-predominant Irritable Bowel Syndrome Rats

SUNJian-mei, GULi-mei, LIHui, HUAQiu-wen, TIANYao-zhou

2017, 33(5): 524-528.

[Abstract](1448) [PDF 593KB](1238)

Abstract:
OBJECTIVE To observe the effects of Yunpi Rougan Fang on serum and colon tissue serum serotonin（5-HT）， vasoactive peptide（VIP） and substance P（SP) in constipation-predominant irritable bowel syndrome rats， to explore its possible mechanism and relevance. METHODS 42 SPF Wistar rats， Six rats were randomly selected as normal group，the remaining 36 rats were modeled by ice water for 14 d，evaluation model， 30 rats were randomly selected and randomly divided into Chinese medicine high， medium and low dose group， western medicine group， model control group， each group had 6 rats. The Chinese medicine low， middle and high dose groups were treated with 12.2， 24.4， 48.8 g/（kg·d)， Mosapride group with moxapril suspension 1.35 mg/（kg·d) gavage，model control group and blank group were given equal amount of distilled water to fill the stomach.After 14 d， the levels of 5-HT， VIP and SP in the serum and the levels of 5-HT and VIP in the colon tissue were measured. RESULTS Compared with the blank group， the expression of 5-HT and VIP in serum and colon tissue immunization in the model control group was increased significantly（P<0.01），the expression of SP in serum was decreased （P<0.05）.Different doses can decrease the levels of serum 5-HT and VIP in C-IBS model rats (P<0.01)， significantly increased the serum SP level (P<0.05). Immunohistochemical results showed that the expression of 5-HT in colon tissue was significantly decreased (P<0.05)， and the expression of VIP was significantly decreased (P<0.01). Although the expression of each treatment group no significant difference (P>0.05)， from the numerical changes， Yunpi Rougan Fang was superior to mosapride and showed dose-dependency. CONCLUSION Yunpi Rougan Fang can obviously improve the symptoms of constipation in C-IBS model rats， by reducing the expression of 5-HT and VIP， improving the expression of SP to regulate intestinal function， which plays a therapeutic role in C-IBS.

Professor ZHOU Zhongying's Experience in Treating Colon Cancer from Spleen Deficiency and Stomach Weakness， Damp Heat Accumulation and Turbid Stagnation

ZHANGXi-lei, HUOJie-ge

2017, 33(5): 529-531.

[Abstract](1483) [PDF 338KB](1494)

Abstract:
This paper introduces professor ZHOU Zhongying's experience for differentiating and treating colon cancer， which includes spleen deficiency and stomach weakness， damp heat accumulation and turbid stagnation. During the clinical syndrome differentiation， professor ZHOU Zhongying combines with other compliant syndromes such as qi stagnation， blood stasis， phlegm retention， collaterals disorders due to the long-term illness， and usually takes strengthening spleen resolving phlegm as the main treating methods. He also takes fighting against cancer and purifying toxin as the method of differentiating disease， and takes regulating the flow of qi and smoothing stagnation， removing phlegm and resolving hard masses， promoting blood circulation to remove blood stasis， seeking and activating collaterals as the referential principles. He especially stresses on the principle of clearing away heat and removing turbidity.

XU Shoutian's Experience in Diagnosis and Treatment of Leukorrheal Diseases

XIE Yue-bo

2017, 33(5): 532-534.

[Abstract](1295) [PDF 356KB](1399)

Abstract:
This paper summarizes professor XU Shoutian's experience in diagnosis and treatment of leukorrheal diseases， which believes that the main pathogenesis of leukorrheal diseases is conception vessel and belt vessel losing control due to the dampness lingering in the lower Jiao impairing conception vessel and belt vessel. And deficiency in nature and excess in superficiality are the pathogenesis features of leukorrheal diseases. The treatment takes eliminating dampness as the main method， and draining dampness as an assisting method. The treatment should be verified according to the ages. The main formula is modified Simiaosan.

Clinical Observation on Modified Sheganmahuang Tang Plus Symbicort in Treating the Acute Attack of Asthma-Chronic Obstructive Pulmonary Disease Overlap Syndrome

WANGShu-min, MAJian, JINYa-ming

2017, 33(5): 535-537.

[Abstract](1306) [PDF 359KB](1314)

Abstract:
OBJECTIVE To observe the clinical effect of modified Sheganmahuang Tang plus symbicort in treating the acute attack of asthma-chronic obstructive pulmonary disease overlap syndrome(ACOS). METHODS Forty-two ACOS patients were divided into the experimental group (22 cases) and the control group (20 cases) according to the random number. On the basis of conventional western drug symptomatic treatment including oxygen inhalation， stopping cough and resolving phlegm， bronchus dilation， anti-infection， the control group was given symbicort inhalation， and the experimental group was given Sheganmahuang Tang plus symbicort. The treatment course was 4 weeks. the curative effects of both groups were observed. RESULTS In terms of clinical effect， the totally effective rates of the experimental group and the control group were 96.00% and 65.00%， respectively， and the difference between two groups was significant， P<0.05. The FEV1% and FEV1/FVC increased significantly compared with the control group， P<0.05. DLCO in the experimental group increased slightly higher than that in the control group， P>0.05; CAT score (16±2) decreased significantly compared with the control group (20±2); and the ACT score (24±3) increased significantly compared with the control group (16±2)， P<0.05. The reoccurrence rate in the experimental group decreased significantly compared with the control group， P<0.05~0.01. Both groups reported no severe adverse event. CONCLUSION Sheganmahuang Tang plus symbicort achieves satisfactory curative effect in the treatment of acute attack of ACOS with no severe adverse event， which is worthy of spreading.

Clinical Study of Eliminate Disease Enema Decoction in Treatment of Fallopian Tube Obstructive Infertility

ZHANG Xiao-yong, XUE Yun-xia, SHI Pei-jing

2017, 33(5): 538-540.

[Abstract](1153) [PDF 330KB](1205)

Abstract:
OBJECTIVE To study the clinical effects of eliminate disease enema decoction in the treatment of fallopian tube obstructive infertility after laparoscopic surgery. METHODS 144 patients with fallopian tube obstructive infertility met with the study criteria were included and randomly divided into the observation group (78 cases) and the control group (66 cases). The control group was treated with laparoscopic surgery alone， and the observation group was treated with eliminate disease enema decoction after laparoscopic surgery. The decoction was used for 7 d continuously each time after menstruation， and the maximum continuously using period was six courses. The pregnancy status of 2 groups was observed and recorded and statistically analyzed. RESULTS The clinical pregnancy rates in the observation group and the control group within two years were 55.13% and 31.82%， respectively. The difference was statistically significant (P<0.05). The postoperative clinical efficacy within 1a was the best. CONCLUSION Performing the laparoscopic surgery first for patients with fallopian tube obstructive infertility and then treating with eliminate disease enema decoction can significantly improve the patient's clinical pregnancy rate.

2017 Vol. 33, No. 5

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