南京中医药大学学报

Study on Stagnant Heat Syndrome of Wasting-Thirst

WANG Ren-he, SHI Yan

2017, 33(4): 325-327.

[Abstract](1156) [PDF 1013KB](1508)

Abstract:
It is believed that stagnant heat is an important cause of wasting-thirst， and an important factor that influencing the development and changes of wasting-thirst. In the course of wasting-thirst， stagnant heat， as an independent pathogenic factor， runs through the disease. The understanding of the causes of the stagnant heat syndrome of wasting-thirst believes that consumption of five zang-organs is the main cause of the disease， and internal damage caused by the seven emotions and endogenous five pathogenic factors are important pathogenic factors of the disease. The pathogenesis of the disease takes the deficiency of liver and kidney as the root factors， dampness， phlegm and blood stasis as the superficial factors. The treatment must give the consideration to both blood stasis and heat， and the combination of cooling blood and dissipating blood stasis is the key for treatment of the disease.

ZHANG Jianzhai's Eight Therapeutic Methods for Damp-Warm Pathogen Disease

YAOLu, WANGChang, XUJian-yun, HOUJun

2017, 33(4): 328-330.

[Abstract](1289) [PDF 1025KB](1829)

Abstract:
Zhang Jianzhai's therapeutic methods for damp-warm pathogen disease were summarized according to his existing medical records， and eight therapeutic methods were obtained including dispelling wind， removing dampness and harmonizing collaterals， clearing heat and removing dampness， diffusing qi and removing dampness with aromatic herbs， regulating qi activities， dispersing evils by purgation， regulating middle energizer and strengthening the transportation of spleen， relieving dryness， venting and discharging， and nourishing yin and harmonizing the center. Zhang divided damp-warm pathogen disease into three phases， the disease location of the first phase is wèi qì， and that of the second and third phases are qi and ying. Among the eight therapeutic methods， dispelling wind， removing dampness and harmonizing collaterals are wildly used in the first phase， clearing heat and removing dampness， relieving dryness， venting and discharging are wildly used in the second and third phases. Relieving dryness， venting and discharging also can prevent transmission and change of the disease. Diffusing qi and removing dampness with aromatic herbs are used when heat is removed and dampness is retained， which is used for regulating qi activities and dispersing evils by purgation. Regulating middle energizer and strengthening the transportation of spleen are running through the treatment of damp-warm pathogen disease. Nourishing yin and harmonizing the center are used for recuperation after three phases.

Diagnosis and Treatment of Headache from Fluid Retention

LIUGao-hong, GUXi-zhen

2017, 33(4): 331-332.

[Abstract](1149) [PDF 996KB](1841)

Abstract:

Study on MIAO Xiyong's Clinical Medication Characteristics in the Treatment of Diarrhea

DINGLiang, XIAOYan, LIWen-lin, CHENDi-ping

2017, 33(4): 333-334.

[Abstract](1110) [PDF 1001KB](1308)

Abstract:
To study Miao Xiyong's clinical medication characteristics in the treatment of diarrhea through discussing the diarrhea related medications， medical records， as well as relevant discussions in his extant representative works. His clinical medication characteristics include harmonizing the stomach and tonifying the spleen， ascending lucid Yang and inducing urination; he was good at using simple formulas and learning wildly from other's strong points. He was also good at adopting wind-drugs to treat the syndrome of excessive dampness and Dong-feng(a kind of diarrhea caused by experiencing the pathogenic wind)， and applying dual supplementation of spleen and kidney to treat daybreak diarrhea.

Observation on Effect of Shenfu Qiangxin Decoction on Echocardiography and Clinical Effectiveness for Heart Failure with Preserved Ejection Fraction

ZHANGSu-jie, FANGZhu-yuan, ZHANGYi-yan, CHENXiao-dong, GUMing-lin

2017, 33(4): 335-338.

[Abstract](1146) [PDF 1043KB](1564)

Abstract:
OBJECTIVE To study the effect of Shenfu Qiangxin Decoction on echocardiography indexes and clinical improvement for patients with chronic heart failure with preserved ejection fraction (HFPEF) (deficiency of both yin and yang， blood stasis and water retention)， and to investigate its possible mechanism. METHODS 57 patients visited in our hospital and diagnosed as chronic HFPEF (deficiency of both yin and yang， blood stasis and water retention) from Aug. 2014 to Aug. 2015 were selected and randomly divided into the control group (28 cases) and the observation group (29 cases). The control group was given conventional therapy， and the treatment group was treated with Shenfu Qiangxin Decoction on the basis of conventional therapy， and two weeks as a course in both groups. Efficacy evaluation was performed after one course treatment. Indexes changes such as plasma brain natriuretic peptide (BNP)， 6-minute walk test (6MWT) and echocardiography in two groups were observed before and after treatment. RESULTS Plasma BNP and 6MWT in both groups improved after treatment (P<0.05~0.01)， but the observation group decreased more significantly (P<0.05). Echocardiographic indexes showed that the size of left atrial (LAD) in both groups were not significantly improved (P>0.05)， The ratio of early diastolic maximum flow velocity of mitral annulus (E peak)/early diastolic maximum velocity of tricuspid annular interventricular septum and left ventricular lateral wall tissue (Ea) and pulmonary arterial systolic pressure (PSA) of patients in two groups improved significantly(P<0.05~0.01)， change extent of the observation group was more significant compared with the control group (P<0.05). CONCLUSION Shenfu Qiangxin Decoction can effectively improve plasma BNP level， 6MWT， and echocardiography of patients with chronic HFPEF.

Effect of Moxibustion at Different Temperatures on the Difference of Microcirculatory Blood Perfusion at Commonly-used Abdominal Points Area

JIANGYing, DAIWen-jun, JIANGJin-feng

2017, 33(4): 339-343.

[Abstract](1270) [PDF 1059KB](1260)

Abstract:
OBJECTIVE To observe the rules and characteristics of microcirculatory blood perfusion at commonly-used abdominal points area under normal physiological conditions， and compare the effect of moxibustion at different temperatures on microcirculation. METHODS 52 health young volunteers were included (male， 18 cases; female， 34 cases). Laser Doppler flowmetry was applied to measure the skin microcirculation values of their abdominal points including Shenque (CV8)， Huangshu (KI16)， Tianshu (ST25)， Daheng (SP15)， Shuifen (CV9)， Qihai (CV6)， Guanyuan (CV4) and ZhongWan (CV12). Then， Shenque (CV8) and Tianshu (ST25) were selected and intervened by moxibustion at different temperatures (40~43℃ and >43℃， <44℃)， and the moxibustion was performed 7 times continuously， at 2 kinds of temperatures for 2min each time. Effects of moxibustion at different temperatures on kin microcirculation of these two points were observed. RESULTS ①Under normal physiological conditions， microcirculation value of Shenque point (CV8) was obviously higher than that of other points (P<0.01). Microcirculation value of Huangshu point (KI16) was higher than that of Tianshu point (ST25) and Daheng point (SP15). Microcirculation value of Tianshu point (ST25) was higher than that of Daheng point (SP15). ②After moxibustion， both microcirculation values of Shenque point (CV8) and Tianshu point (ST25) increased， and both the differences of mean and maximum microcirculation values of Shenque point (CV8) were higher than that of pre-moxibustion at two temperatures. Under the stimulation of moxibustion， microcirculation value of Shenque point (CV8) changed earlier and reached platform later compared with Tianshu point (ST25). The response of microcirculation value of Shenque point (CV8) was more sensitive to temperature changes than Tianshu point (ST25). CONCLUSION Among commonly-used abdominal points， skin microcirculation value of Shenque point (CV8) is the highest， skin microcirculation values of abdominal points that horizontally paralleled with umbilicus tend to be higher if it is closer to umbilicus. With the same warm moxibustion condition， increasing speed， range and duration of skin microcirculation value of Shenque point (CV8) are higher compared with Tianshu point (ST25). It is preliminary clear that among the commonly-used abdominal points， Shenque point (CV8) has particularity and superiority in microcirculation， which provides effective evidence support for the specificity of the microcirculation structure.

Effects of Zishen Qinggan Formula on Arterial Function and Inflammatory Factors in Patients with Essential Hypertension

LI Yi-zhuo, HUANG Hui, GU Wan-jian, LIU Niu, JI Ming-de, WU Lin-lin, LUAN Yun, YAN Shi-hai, LIU Fu-ming

2017, 33(4): 344-348.

[Abstract](1135) [PDF 1062KB](1164)

Abstract:
OBJECTIVE To evaluate the clinical efficacy of Zishen Qinggan Formula through observing its effects on arterial function and inflammatory factors in patients with essential hypertension. METHODS 100 essential hypertension patients who meet the inclusion criteria were selected and randomly divided into the treatment group and the control group， 50 cases in each group. The treatment group received ACEI/ARB+CCB standard treatment combined with Zishen Qinggan Formula， and the control group received ACEI/ARB+CCB drugs treatment. The course of treatment was 8 weeks. Changes of TCM syndrome scores， carotid intima-media thickness， UltraFast imaging pulse wave velocity (PWV)， resistance index， inflammatory factors and safety indexes were compared before and after treatment. RESULTS ①TCM syndrome scores in both groups improved significantly after treatment， and that in the treatment group was better than the control group(P<0.01). ② UltraFast imaging PWV of bilateral carotid arteries and resistance indexes in both groups improved after treatment (P<0.01)， among which the treatment group was better than the control group(P<0.05). IMT of bilateral carotid arteries had no difference in two groups before and after treatment (P>0.05). ③ Levels of serum CRP， IL6 and TNFα in both group improved after treatment (P<0.05)， and that in the treatment group were better than the control group(P<0.01) ④ There was no significant difference in liver and kidney function in two groups before and after treatment(P>0.05). CONCLUSION In the aspect of improvement of arterial elasticity and inflammatory factors in patients with essential hypertension， western medicine ACEI/ARB+CCB basic therapy combined with Zishen Qinggan Formula is better than ACEI/ARB+CCB basic therapy alone.

Clinical Study of Self-Made Enriching Yin and Subduing Yang， Regulating Liver and Extinguishing Wind Formula in the Treatment of Tourette Syndrome Caused by Wind Stirring due to Yin Deficiency

LUFang, ZHANGXu-sheng, GUQin, WANGYu-ling, YANGXiao-li

2017, 33(4): 349-353.

[Abstract](1269) [PDF 1069KB](1209)

Abstract:
OBJECTIVE To study the clinical effectiveness and influence of Enriching Yin and Subduing Yang， Regulating Liver and Extinguishing Wind Formula in the treatment of Tourette syndrome caused by wind stirring due to yin deficiency. METHODS 80 patients with Tourette syndrome caused by wind stirring due to yin deficiency admitted in our hospital from Oct. 2013 to Oct. 2016 were selected and randomly divided into the observation group and the control group by random number， 40 cases in each group. The control group was treated with inosine combined with vitamin B6， and the observation group was treated with self-made Enriching Yin and Subduing Yang， Regulating Liver and Extinguishing Wind Formula. One month for a course for both groups， and both were treated for 3 courses. Changes of clinical symptoms and signs were observed before and after treatment. YGTSS of American Infant Psychosis Study Institute was applied to test the YGTSS scores in two groups before and after treatment. The clinical effect was evaluated according to the reduction rate of YGTSS scale. Cellular immunity (CD3+， CD4+， CD8+ and CD4+/CD8+) and humoral immunity (serum IgG， IgA and IgM) levels in two groups were tested. Patients in two groups were followed up for 3 months to count the recurrence. RESULTS Total scores of TCM syndrome in two groups decreased significantly (P<0.01) after treatment， but that in the observation group decreased more significantly (P<0.01). Motor tics， incidence tics， resultant damages and YGTSS scores of total in two groups decreased significantly (P<0.01) after treatment， but that in the observation group decreased more significantly (P<0.01).Total effective rate in the observation group was 97.5%， which was significantly higher than that in the control group (85.0%， P<0.05). The recurrence rate of the observation group (5%) significantly lower than that in the control group (20.00%)(P<0.05). CD3+， CD4+， CD8+ and CD4+/CD8+ in two groups improved significantly after treatment (P<0.01)， but that in the observation group improved more significantly (P<0.01). IgA， IgG and IgM levels in two groups increased significantly after treatment (P<0.01)， but that in the observation group increased more significantly (P<0.01). CONCLUSION Enriching Yin and Subduing Yang， Regulating Liver and Extinguishing Wind Formula can significantly improve clinical symptoms of patients with Tourette syndrome， decrease patients， incidence tics， motor tics and resultant damages， which have significant clinical effectiveness and high safety. Its action mechanism may be related to the improvement of immunity.

Clinical Research on Dachaihu Granule in Treating Chronic Cholecystitis Due to Stagnant Heat in Liver and Gallbladder

CHENJing, SHENHong, LINYue-han, LIAOHeng-xiang, JIANGYi-ping, WANGChui-jie, JINXiao-jing, SUJuan-ping, SHAOFeng-zhen, ZOUJian-dong, LIUFang

2017, 33(4): 354-358.

[Abstract](1302) [PDF 1060KB](1350)

Abstract:
OBJECTIVE To evaluate the validity and safety of Dachaihu Granule in treating chronic cholecystitis due to Stagnant Heat in Liver and Gallbladder. METHODS A stratified-block randomized， double-blind， double-dummy， parallel， placebo-controlled trial was designed， with 600 cases with chronic cholecystitis from 8 centers being selected and the ratio of treatment group， placebo group and cholagogic tablet group being 360∶120∶120. Patients in different groups were separately given Dachaihu Granule and cholagogic tablet simulator， Dachaihu Granule simulator and cholagogic tablet simulator， Dachaihu Granule simulator and cholagogic tablet. Then the validity and safety were assessed after 7 days' treatment. RESULTS ①In terms of the comprehensive curative effect of TCM syndromes， it was noticed that there existed no difference between the treatment group and positive medicine group in the integral reduction and disappearance of upper quadrant pain and tenderness， quadrant pain relief evaluated by patients， total symptom scores， integral reduction and disappearance of bitter taste， thirst， constipation and yellow urine， disappearance of vomit， change of gallbladder wall thickness and ultrasonic Murphy's character before and after the treatment， while both the treatment group and positive medicine group were superior to the placebo group(P<0.05~0.01). ②The treatment group and positive medicine group has no difference from the placebo group in integral reduction and disappearance o ffever and nausea integral reduction of vomit， change of gallbladder size and inside diameter of common bile duct. ③Good safety was noticed. CONCLUSION Chronic cholecystitis due to stagnant heat in liver and gallbladder treated by Dachaihu Granule is safe and effective.

Clinical Effect Observation of Jianpishugan and Huoxuehuatan Formula on Liver Depression and Spleen Deficiency Induced Non-Alcoholic Steatohepatitis

HU Yong, MOU Hai-jun, CHEN Ke

2017, 33(4): 359-362.

[Abstract](1139) [PDF 1041KB](1096)

Abstract:
OBJECTIVE To study the clinical effect of Jianpishugan and Huoxuehuatan formula on liver depression and spleen deficiency induced non-alcoholic steatohepatitis. METHODS 140 patients with liver depression and spleen deficiency induced non-alcoholic steatohepatitis admitted in our hospital from Oct. 2015 to Oct. 2016 were selected and randomly divided into the observation group and the control group according to the random number. Both groups were given exercise and diet therapy， and the control group was also treated with polyene phosphatidylcholine capsules. Based on the control group， the observation group was also treated with modified Jianpishugan and Huoxuehuatan Formula. RESULTS Symptom scores and the total scores of the clinical symptoms in two groups decreased significantly (P<0.01) after the treatment， but that in the observation group decreased more significantly (P<0.01). Total effective rate of overall efficacy in the observation group was 94.28% after the treatment， which was significantly higher than that in the control group (77.14%， P<0.01). Total effective rate of TCM syndrome in the observation group was 95.71% after the treatment， which was significantly higher than that in the control group (77.14%， P<0.01). ALT， AST and GGT levels in two groups decreased significantly (P<0.01) after the treatment， but that in the observation group decreased more significantly (P<0.01). Abdominal B ultrasound results in two groups improved significantly after the treatment(P<0.01)， but that in the observation group improved more significantly (P<0.01). During the treatment， patients in two groups did not complain of other discomforts， adverse reactions and toxic effects. CONCLUSION Jianpishugan and Huoxuehuatan Formula can noticeably improve the clinical symptoms and liver functions of patients with liver depression and spleen deficiency induced non-alcoholic steatohepatitis.

Clinical Observation of Moxibustion on Acupoint Feishu for Treatment of Acute Asthma in Children (Cold-Asthma)

QIANHai-liang, XIYue, CHENGZhi-kun, WANGShi-you, SHIJin-mei, CHENRi-xin

2017, 33(4): 363-366.

[Abstract](1282) [PDF 1037KB](1490)

Abstract:
OBJECTIVE To observe the clinical effect of moxibustion on acupoint Feishu (BL13) in the treatment of acute asthma in children (cold-asthma)， and to provide clinical evidence for treatment of asthma with acupuncture and moxibustion. METHODS 87 children with asthma (cold-asthma) who meet the inclusion criteria were selected and randomly divided into 2 groups. The control group was treated with conventional western medicine， and the observation group was treated with moxibustion on bilateral Feihu Points(BL13) on the basis of routine western medicine. CRP， IgE， IL1， IL6， EOS， pulmonary function and symptom score were selected as observation indexes for the clinical effects comparison of patients in two groups. RESULTS Indexes levels such as CRP， IgE， IL6 and EOS in the observation group were significantly lower than that in the control group after treatment (P<0.05~0.01). FEV1 and PEF levels of patients in the observation group were significantly better than that in the control group after treatment (P<0.05). Symptom score in the observation group was significantly lower than that in the control group after treatment (P<0.05). Apparent rate (73.81%) of patients in the observation group was significantly higher than that in the control group (53.33%) (P<0.05). CONCLUSION Moxibustion on acupoint Feishu in the treatment of acute asthma in children (cold-asthma) can markedly decrease the levels of CRP， IgE， IL6 and EOS in patients， improve pulmonary function， and can achieve remarkable clinical curative effect.

Clinical Effectiveness Observation of the Combination of Tuina and Functional Exercise in the Treatment of Knee Osteoarthritis

XIE Hong-bo

2017, 33(4): 367-370.

[Abstract](1111) [PDF 1051KB](1202)

Abstract:
OBJECTIVE To observe the clinical effectiveness of the combination of Tuina and functional exercise in the treatment of knee osteoarthritis (KOA). METHODS 180 KOA patients admitted in our hospital from Jan. 2016 to Dec. 2106 were selected and randomly divided into the observation group A， B and the control group by random number， 60 cases in each group. The control group was intervened by normalized functional exercise， the observation A group was intervened by Tuina with Chinese medicines characteristics on the basis of the control group， and the observation group B was treated with glucosamine sulfate capsules on the basis of the control group. Scores of knee-joint swelling， tenderness， joint activity and Lysholm knee function were evaluated before and after treatment. Knee joint fluid was extracted from patients and Matrix metalloproteinase-3 (MMP3)， tissue inhibitor of metalloproteinase 1 (TIMP1) and tumor necrosis factor-α (TNF-α) were tested before and after treatment. The clinical efficacy after treatment and safety during treatment were evaluated in two groups. RESULTS Scores of knee-joint swelling， tenderness and joint activity in three groups significantly decreased after treatment (P<0.05)， and Lysholm knee function scores increased significantly (P<0.05)， but scores of knee-joint swelling， tenderness and joint activity in observation groups A and B decreased more significantly (P<0.05)， and Lysholm knee function scores increased more significantly (P<0.05). Levels of MMP3， TIMP1 and TNF-α in three groups decreased significantly after treatment(P<0.05)， but observation groups A and B decreased more significantly(P<0.05). Total effective rates in observation groups A and B were 95.00% and 1.67%， respectively， which were significantly higher than that in the control group (75.00%)， the differences were statistically significant (2=9.412、8.757， P<0.01). There were no obvious adverse reactions in three groups， and the safety was grade I. CONCLUSION The combination of Tuina and functional exercise has significant clinical effectiveness and high safety in the treatment of KOA， which is worthy of clinical promotion.

Influence of Phospholipid Composition on the Permeation Behaviors of Active Ingredients from Xiang-Fu-Si-Wu Decoction Through Liposome-Based Artificial Skin Membrane

TANG Qing-han, ZHANG Hui, SHEN Jing-jing, JIANG Wen-yi, SHEN Hao-wen, LIU Lu, MA Min, GU Wei, CHEN Jun

2017, 33(4): 371-375.

[Abstract](1229) [PDF 1214KB](1080)

Abstract:
OBJECTIVE To investigate the effects of the composition of egg phosphatidylcholine (EPC) and hydrogenated soybean phosphatidylcholine (HSPC) on the permeation behaviors of active ingredients from Xiang-Fu-Si-Wu Decoction (XFSWD) through liposome-based artificial skin membrane (LASM). METHODS Liposomes with different phospholipid compositions were prepared by film-dispersion method. The nylon membrane was combined with liposomes by freezing-thawing cycle to obtain LASM and its electrical resistance value was measured by a cell membrane resistance meter. The LASM with different phospholipid compositions was applied for the measurement of skin permeation behaviors of five active ingredients （ferulic acid， paeoniflorin， albiflorin， tetrahydrocolumbamine and tetrahydropalmatine） from XFSWD. Interaction mechanism between EPC and HSPC was investigated by differential scanning calorimetry (DSC) analysis. RESULTS Phospholipid composition played a regulatory role for the electrical resistance of LASM. The highest barrier function of LASM was obtained at the molar ratio of EPC∶HSPC=3∶1， resulting in the lowest permeation values of the active ingredients from XFSWD. The permeability increased with the increase of HSPC in LASM in the range of the molar ratio of EPC∶HSPC =3∶1~1∶3. Additionally， DSC analysis revealed that EPC and HSPC could be combined into one homogenous phase at the molar ratio of EPC∶HSPC =3∶1. CONCLUSION The barrier function of LASM can be effectively controlled by the modification of phospholipid composition， facilitating the simulation of real skin barrier to investigate the skin permeation behaviors of the active components from traditional Chinese medicine.

Therapeutical Effect of Dihydroartemisinin on Bile Duct Ligation-Induced Liver Fibrosis in Rats and Its Mechanism

XUXia, FUJin-bai, YAOZhen, CHENYi-fan, QIANLei, JIANGShuo-yi, ZHOUJing-yi, ZHENGShi-zhong

2017, 33(4): 376-381.

[Abstract](1233) [PDF 1596KB](1184)

Abstract:
OBJECTIVE To investigate the therapeutical effect of dihydroartemisinin on bile duct ligation-induced liver fibrosis in rats. METHODS Hepatic fibrosis model was established by bile duct ligation， treatment groups in which rats received intraperitoneal injection of DHA at 14 mg/kg and colchicine treatment. At the end of the experiment， the rats were sacrificed， Blood was collected to exam Levels of alanine aminotransferase (ALT)， aspartate aminotransferase (AST)， and Bilirubin in serum samples. Hematoxylin and eosin (HE)， Masson’ s trichrome stain and sirius red collagen staining were used to Pathological changes in liver tissue. Western blotting analyses were used to evaluate the indicators related with HSC activation such as α-SMA， α 1(Ⅰ) collagen in liver tissue. Immunofluorescence was used to evaluate the expression of TGFβ-RⅡ in liver tissue. HSC-T6 was cultured in vitro and treated with DHA. Western blotting analyses were used to evaluate the expressions of α-SMA， α 1(Ⅰ) collagen， TGFβ-RⅡ， p-Smad2， and Smad2. RESULTS As shown in Serological indicators， compared with the control group， contents of serum ALT， AST， Bilirubin was significantly increased in the model group， DHA could decrease the serum levels of ALT， AST， Bilirubin. As shown in HE， Masson， and Sirius red staining， comparedwith the model group， DHA significantly alleviated liver pathology tissue and deposition of collagen fibers， and inhibited expression of α-SMA， α 1(Ⅰ) collagen and TGFβ-RⅡ， p-Smad2. CONCLUSION DHA had the protective effect on liver fibrosis induced by BDL， which was primarily related to inhibition of HSC activation and regulation of TGF -βII/Smad2 signaling pathways.

Study on Mechanism of Loganin Against AGEs Induced GMCs Injury via AGEs/RAGE/SphK1 Pathway

LYUXing, XUHui-qin, LYUGao-hong, WUYun-hao, CHENYu-ping, SHENHong-sheng, DAIGuo-ying, XUKang

2017, 33(4): 382-385.

[Abstract](1057) [PDF 1438KB](1193)

Abstract:
OBJECTIVE To study the mechanism of loganin， the effective componet of Cornus officinalis， on protecting the glomerulus mesangial cell (GMCs) injured by advanced glycation end products (AGEs). METHODS GMCs were divided into control， model (AGEs: 200mg/L)， loganin (0.1， 1， 10μmol/L) groups. The cell proliferation， FN and COL-Ⅳ secretion， microstructure lesions， RAGE， SphK1， S1P and TGF-β protein expressions were measured. RESULTS AGEs induced cell proliferation， FN and COL-Ⅳ secretions， microstructure lesions， RAGE， SphK1， S1P and TGF-β protein expressions were suppressed by loganin. CONCLUSION Loganin can improve the AGEs induced GMCs injury by regulating the AGEs/RAGE/SphK1 signal pathway， and then ameliorate the diabetic nephropathy.

The Research of Continuous Illumination on Reproductive Endocrine of Rats and the Intervention Effect of Nourishing Yin and Tonifying Yang Fang Sequential Traditional Chinese Medicine

ZHANGYu-jie, MAQian-wen, TANYong

2017, 33(4): 386-390.

[Abstract](1181) [PDF 1468KB](1151)

Abstract:
OBJECTIVE To discuss the effect of continuous illumination on reproductive endocrine and behavior of rats and intervention effect of Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine. METHODS Normal control group rats were under the light condition of 12/12 h light/dark cycle. Model group rats were under the light condition of 24h continuous illumination. Light intensity were chosen (300±20)lux. The time of building model was 50 days. The model assessment was according to the situation of estrus cycles. After eliminating unsuccessful models， residual model rats were randomly divided into model group and traditional Chinese medicine group. Control group and model group were given saline lavage. Traditional Chinese medicine group was given Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine treatment. Three groups of rats had forced swimming test and the sugar water preference experiments after the treatment. They were compared the fixed time and sugar water preference values. Three groups of rats were measured serum E₂(Estradiol)， FSH(Follicle Stimulating Hormone)， LH(Luteinizing Hormone)， GnRH(Gonadotropin-Releasing Hormone)， MT(Melatonin) and 5-HT(5-Hydroxy Tryptamine) after the treatment and compared between the three groups. The ovarian tissues of three groups， rats were observed under the electron microscope after the treatment. The difference of ovarian ultrastructure was compared among the three groups. RESULTS In the sugar water preference experiments， the sugar water preference values of the model group and the traditional Chinese medicine group were obviously lower than the control group. The sugar water preference values of the traditional Chinese medicine group rats were higher than the model group rats. In the forced swimming test， the fixed time of the model group and the traditional Chinese medicine group rats was higher than the control group rats. The fixed time of the traditional Chinese medicine group rats was lower than the model group rats. The level of E₂(Estradiol)， FSH(Follicle Stimulating Hormone)， LH(Luteinizing Hormone) and MT(Melatonin) of the model group and the traditional Chinese medicine group were lower than the control group. The level of FSH(Follicle Stimulating Hormone) and MT(Melatonin) of the traditional Chinese medicine group were obviously higher than the model group. However， there was no difference between the traditional Chinese medicine group and the model group on the level of E₂(Estradiol) and LH(Luteinizing Hormone). At the same time， the level of 5-HT(5-Hydroxy Tryptamine) of the model group was higher than the control group. The level of 5-HT(5-Hydroxy Tryptamine) of the traditional Chinese medicine group was significantly lower than the model group. However， there was no significant difference between the control group and the traditional Chinese medicine group. From the ultrastructure angle， there was visible damage in the model group and the traditional Chinese medicine group of ovarian cells compared with the control group. Furthermore， the injury of the model group was more serious than the traditional Chinese medicine group. CONCLUSION Continuous illumination may lead to mood disorders of rats， restraining the reproductive function and sex hormone level disorders. Nourishing Yin and Tonifying Yang Fang sequential traditional Chinese medicine can improve the mood and reproductive function of rats in a certain degree.

Study on Antiblastic Effect Mechanism of ER(+) Human Breast Cancer MCF-7 Cells by Triptolide Based on CYP19

LIUMin, ZHANGLu-rong, LIANGGuo-qiang, JIANDun-yu, ZHANGXiao-di

2017, 33(4): 391-394.

[Abstract](1142) [PDF 1397KB](1295)

Abstract:
OBJECTIVE To investigate the mechanisms of Triptolide in ER(+) human breast cancer MCF-7 cells from CYP19. METHODS The inhibition of the MCF-7 cells influenced by Triptolide and Letrozole was analyzed respectively by MTT assays. The aromatase levels were measured by Western blot. The CYP19 gene was observed by RT-PCR. The MCF-7 cells of high and low expression CYP19 gene were transfacted. It's CYP-19 and downstream channel gene JNK，p-38 and ERK were observed by Western blot. RESULTS Triptolide displayed a dose- and time-dependent inhibition of the MCF-7 cells. It inhibited the aromatase and CYP19. The expression of CYP19 in MCF-7 cells of low expression CYP19 was significantly decreased.Phosphorylation of JNK，p38 and ERK was inhibited too. CONCLUSION Triptolide inhibit MCF-7 cells not only via inhibition aromatase， but also blocking Ras-Raf-MAPK-ERK enzyme.

Effects and Mechanisms for Guilu Erxian Glue on Dexamethasone Induced Bone Marrow Mesenchymal Stem Cells Injury

XUWei-feng, FEIYi-ming, WANGJian-wei, YINHeng, WANGShan-fu, GEWei-hong

2017, 33(4): 395-398.

[Abstract](1137) [PDF 2925KB](1185)

Abstract:
OBJECTIVE The protective effect of Guilu Erxian Glue on dexamethasone induced bone marrow mesenchymal stem cells injury. METHODS Bone marrow mesenchymal stem cells were obtained from SD rats and divided into four groups: normal group， dexamethasone (10-8) group， low dose and high dose of Guilu Erxian Glue groups. The activity of bone marrow mesenchymal stem cells was measured by MTT assay， superoxide dismutase (SOD)， malondialdehyde (MDA)， interleukin-6 (IL-6)， IL-1β， tumor necrosis factor a (TNF-α) were measured. The expression of Nrf-2/HO-1/NF-κB signal proteins was detected by Western blot. RESULTS Guilu Erxian Glue groups can significantly improve cell vitality， increase SOD level， reduce the level of MDA， reduce the level of inflammation factor， increase the expression of Protein， Nrf-2 and HO-1 proteins， and reduce the expression of NF-κB signal proteins. CONCLUSION Guilu Erxian Glue can inhibit the injury of bone marrow mesenchymal stem cells by dexamethasone， and the role is related to the regulation of Nrf-2/HO-1/NF-κB signal.

Effects of BoFa on Expression of IL-6 and SOCS3 in Spinal Cord in CCI Rats

MA Chi, YAO Bin-bin, YU Tian-yuan, TAO Yan-hong, LU Meng-qian, JIA Wen-duan, ZHANG Lin-feng, GUO Xin

2017, 33(4): 399-402.

[Abstract](1103) [PDF 2109KB](1150)

Abstract:
OBJECTIVE To explore the effect of BoFa for neuropathic pain in rats from the perspective of inflammatory reaction. METHODS The CCI model rats were intervened qualitatively and quantitatively by the massage simulation instrument. Observe the variation of rats' behavior though solar-thermal pain threshold resistance. Observe the variation of IL-6 and SOCS3 in spinal cord in rats through Immunohistochemical technique. RESULTS After seven days of surgery， the results of solar-thermal pain threshold resistance of the model group were significance changed than that of normal group(P<0.01)， the results of IL-6 and SOCS3 in spinal cord in rats were significantly increased than that of normal group(P<0.01). After twenty times of BoFa therapy， the results of solar-thermal pain threshold resistance of the BoFa group were significance changed than that of model group(P<0.05)， and near normal group level; the results of IL-6 in spinal cord in rats were significantly reduce than that of model group(P<0.01); the results of SOCS3 in spinal cord in rats were significantly increased than that of model group(P<0.01). CONCLUSION BoFa can improve the pain sense and temperature sense of CCI rats in a certain extent， and it also can inhibit the expression of pro-inflammatory factors in rats.

Effect of Enteromorpha Polysaccharides of Jiangsu Province on Alloxan-Induced Diabetic Mice

LIUQian-yang, SHUYe-ting, LIURui, WANGXin-zhi, CHENYi-ping, MAXiao-ming, YUANMin, CUIXiao-bin, CHENGJian-min

2017, 33(4): 403-407.

[Abstract](1220) [PDF 1215KB](1082)

Abstract:
OBJECTIVE Study the effects of Enteromorpha polysaccharides of Jiangsu province on alloxan-induced diabetic mice was studied. METHODS The diabetic mice were induced by alloxan ip， and treated with distilled water (model group)， Shenqi Jiangtang granule (500 mg/kg)， three different doses of (100， 200， and 400 mg/kg) for 28 days respectively. The changes of body weight， food and water intake were observed during administration. The content of fasting blood glucose， oral glucose tolerance， glycosylated serum albumin and serum insulin levels were measured after 28 days of treatment. The pancreatic tissues were taken for pathological observation， the islets numbers and islet area were also determined. RESULTS Medium and high dose group of can significantly reduce the fasting blood glucose level (P<0.01)， decrease the area under the curve of blood glucose (P<0.01)， increase the number of islets and islet area (P<0.01)， elevate serum insulin level (P<0.05~0.01) and high dose of can significantly reduce the content of glycosylated serum albumin (P<0.05) as compared with the diabetic model mice. CONCLUSION Within the 200~400 mg/kg dose range of can decrease blood glucose， improve glucose tolerance and relieve the pancreatic lesion levels of diabetic model mice. The results will provide a theoretical basis for the development of drugs or functional health care products of Enteromorpha polysaccharides of Jiangsu province

Mechanism and Antibacterial Activity of Tibet Medicine Liuweidingxiang pills against Staphylococcus aureus

HEPing, ZHUXiao-li, DAWATsering, WANGShi-hua, HEShu-mei, YUANDong-ya, WANGJin-bo

2017, 33(4): 408-411.

[Abstract](1280) [PDF 1471KB](1100)

Abstract:
OBJECTIVE To investigate antibacterial activity and mechanism of Tibetan medicine Liuweidingxiang pills against Staphylococcus aureus， and provide theoretical basis for clinical application. METHODS M-H agar-punching method was adopted to research antibacterial activity of Liuweidingxiang pills against Staphylococcus aureus， and the minimum inhibitory concentration (MIC) was measured by broth dilution method. The absorbance OD_600nm of culture medium mixed with medicine was continuously recorded for 36h and growth curve of S. aureus was drawn. Furthermore， the antibacterial mechanism was explored using scanning electron microscopy and cell wall permeability test. RESULTS The diameter of inhibition zone was (21.8±2.36) mm at 800 mg/mL， and the MIC was between 12.5 mg/mL and 25 mg/mL. This medicine had significant inhibition to bacterial proliferation. The structure of bacterial cell was changed and the permeability of cell wall was increased. CONCLUSION Liuweidingxiang pills had significant antimicrobial effect on multidrug-resistant S. aureus， which implied potential value in clinical application.

Design， Synthesis and Druggability Evaluation of Liguzinediol Valine Ester Prodrug as Promissing Inotropic Agent

LI Zhi, SHEN Min-zhe, LI Wei, WEN Hong-mei, LIU Jian, ZHU Hao-hao, QIAN Cheng-bo, CAI Jian-guo

2017, 33(4): 412-416.

[Abstract](1524) [PDF 1094KB](1392)

Abstract:
OBJECTIVE One liguzinediol valine ester prodrug was synthesized and evaluated for the physicochemical properties and bioconversion， which laid the foundation for further study of liguzinediol amino acid ester prodrugs. METHODS Liguzinediol valine ester prodrug was prepared by a two-step reaction of condensation and deprotection from liguzinediol， and its structure was verified by LC-HRMS， 1H-NMR and 13C-NMR. The chemical stability， capacity factor， solubility， lipophilicity， metabolic stability in human plasma and pharmacokinetics study in vivo of valine ester prodrug were tested by HPLC. RESULTS Liguzinediol valine ester prodrug retained great solubility and showed good bioconversion in human plasma. The half-time of liguzinediol was extended obviously. However， the lipophilicity was relatively poor. CONCLUSION Liguzinediol valine ester prodrug was provided with the feature to form the drug， and obvious extended the half-time of liguzinediol. Meanwhile， it indicated that prodrug strategy was feasible， which provided the way of experiment for liguzinediol prodrug research.

Identification of Fritillaria powder by IR and scanning electron microscopy

LIU Fang, ZHENG Yi-lei, WANG Xiao, LIU Hong-mei, FANG Cheng-xin, GUO Li

2017, 33(4): 417-419.

[Abstract](1250) [PDF 6990KB](1332)

Abstract:
OBJECTIVE To identify the Fritillaria powder and adulterant. METHODS FIR and scanning electron microscopy were adopted to identify Fritillaria powder and mixture with starch and rice flour. RESULTS The IR one-dimensional spectrum of starch and rice flour was similar to Fritillaria powder， while the two-dimensional spectrum was different. The spectrum of starch and rice flour at 1489cm-1 and 669cm-1 respectively showed difference. The scanning electron microscopy spectrum of Fritillaria powder was also different from mixture with starch and rice flour. CONCLUSION FIR and scanning electron microscopy can identify the Fritillaria powder and adulterant intuitively and effectively and it provide reference for the quality evalution of the Fritillaria powder.

Analysis on Prescription Rules of Stroke Prescriptions in Different Dynasties Based on Data Mining Techniques

CHENChu-yun, LILi-xia, ZHUWei-feng, LINGuo-hua, LIWei-lin, HUANGYing-jie, WANGXing

2017, 33(4): 420-424.

[Abstract](1119) [PDF 1047KB](1131)

Abstract:
OBJECTIVE To excavate and analyze 150 ancient acupuncture classics from Tang Dynasty to Qing Dynasty by data mining technology， and summarize points selection characteristics of acupuncture prescriptions for stroke in different dynasties. METHODS Standard library and ancient classics library were set up firstly. Then extract the related statements from classics that corresponding with symptoms， points， meridians， needling and moxibustion methods of stoke， and to form the related library about symptoms， points， meridians， needling and moxibustion methods. The prescriptions were divided into two categories including prescriptions for stroke and prescriptions that mainly containing points for stoke， which were reviewed and standardized manually. Multilevel analysis of acupuncture prescription rules for stroke in different dynasties were realized through statistical analysis and support and confidence of association rules. RESULTS A total of 536 acupuncture and moxibustion prescriptions for stroke were obtained. The use frequency of Baihui points (GV20) was the highest in all dynasties. The compatibility of Baihui(GV20) and Quchi(LI11) was used most frequently in Song， Ming and Qing Dynasties. The compatibility of Dicang (ST4) and Jiache (ST6) was used most frequently in Yuan Dynasty. For meridians selections， apart from the hand jueyin pericardium meridian was selected as yin meridian in Qing Dynasty， the first five meridians in all other dynasties were yang meridians. The governor vessel and the foot shaoyang gallbladder meridian were the most frequently selected meridians in Song and Yuan Dynasties. The hand taiyang large intestine meridian was the most frequently selected meridian Ming and Qing Dynasty. In all dynasties， the support of hand taiyang large intestine meridian and the foot shaoyang gallbladder meridian were the highest. The confluence points were the first selection among specific points， except Qing Dynasty， the compatibility of the confluence points and he-sea points was used most frequently. CONCLUSION In acupuncture and moxibustion classics， the yang convergence Baihui point (GV20) is the first selection in the treatment of stroke. The application of yang meridians and specific points are valued. The point application is fewer but better in ancient time， while later generations gradually increase the number of points.

Study on Syndrome Differentiation Rules of Ding Ganren's Case Records in External Medicine

YULing, WANGYing-xiao, LIQi-zhong

2017, 33(4): 425-427.

[Abstract](1197) [PDF 1104KB](1208)

Abstract:
OBJECTIVE To analyze the syndrome differentiation rules of Ding Ganren's case records in external medicine. METHODS Association rules analysis on Ding's case records in external medicine was performed with modeling established by Apriori algorithm. Disease and syndrome differentiation characteristics of Ding's case records in external medicine was explored combined with conventional literature research and frequency statistics. RESULTS Cellulitis was a dominant disease in Ding's case records in external medicine， abscess， maxillary osteomyelitis and scrofula were also common. Compared with the syndrome differentiation in Chinese Traditional Surgery， Ding paid more attention to internal syndrome rather than identified the interior and exterior in the syndrome differentiation of cellulitis. Ding took exterior excess heat syndrome as the main syndrome of infectious swollen head rather than interior heat syndrome. In addition， the cases that were differentiated as blood deficiency and qi stagnation or yin deficiency and qi stagnation were more common than conventional external syndromes such as qi stagnation and blood stasis，phlegm and blood stasis binding and dual deficiency of qi and blood. CONCLUSION The differences of the common surgical diseases syndrome differentiation of Ding and Chinese Traditional Surgery reflect Ding's unique experience in exterior and interior syndrome differentiation. The coexistence of yin and blood deficiency and qi stagnation usually occurs in Ding's case records in external medicine such as tumor， cancer， scrofula and suppurative osteomyelitis， which reflects Ding's understanding about lesion mechanisms of "deficiency resulting in stagnation" and "stagnation resulting in deficiency". This enriches the TCM theory of relationship between qi and blood， expands the thinking of TCM syndrome differentiation in external medicine.

Advanced Study on Chemical Constituents and Pharmaceutical Activities of Xanthium Strumarium

ZHUANGYan-shuang, HUJing, CAIHao, QINKun-ming, YANGBing, LIUXiao, CAIBao-chang

2017, 33(4): 428-432.

[Abstract](1708) [PDF 1093KB](2701)

Abstract:
Xanthium strumarium is a common Chinese medicine used for the treatment of “Bi Yuan”， it mainly contains water-soluble glycosides， sesquiterpene lactones， essential oils， fatty oils， phenolic acids and other compounds， its pharmacology contains hypoglycemic， antianaphylaxis， anti-microbial， anti-inflammatory， analgesia and anti-tumor. This article summarized the chemical composition and pharmacological effects of Xanthium strumarium in order to provide reference for later study.

2017 Vol. 33, No. 4

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