南京中医药大学学报

Chinese Medicine Master Qiu Peiran's Diagnosis and Treatment Strategy in Liver Cirrhosis

WANG Qing-qi, LI Xiao-gang, ZOU Chun-pu, LIANG Shang-hua, WANG Shao-mo, QIU Shi-ke

2017, 33(3): 217-220.

[Abstract](1582) [PDF 1025KB](1834)

Abstract:
The Chinese medicine master Qiu Peiran， good at the treatment of liver cirrhosis， believes that the etiology of liver cirrhosis covers exogenous pathogenic factors， infection of parasite， excess alcohol and food， emotional depression， deficiency of vital qi， and its primary pathogenesis is the deficiency of vital qi and lingering of evil qi， including coexistence of yin deficiency and heat-dampness， blood heat and blood stasis， and disease in liver and spleen. The diagnosis and treatment strategy of liver cirrhosis is summed up according to the clinical experience of Prof. Qiu， involving applicable diseases， basis of syndrome differentiation， therapeutic principle and method， prescription and evaluation index of efficacy， etc.

Exploration and Analysis of Wang Xugao's Academic Thoughts on Differentiation and Treatment of Consumptive Disease

ZHANGZhi-feng, WUMeng-yuan

2017, 33(3): 221-222.

[Abstract](1317) [PDF 990KB](1567)

Abstract:
It is believed that Wang Xugao's differentiation and treatment of consumptive disease is mainly based on the theory of correspondence between man and nature and the unity of zang-fu organs， which always comes the top priority when it comes to the syndrome differentiation. Regulation and tonification of middle earth and harmony among zang-fu， qi and blood are emphasized in the treatment， especially in the tonification of the foundation of acquired constitution， with Zhang Zhongjing an Li Dongyuan's prescriptions being frequently applied.

Study on Migraine Treatment from the View of Blood Deficiency and Liver Hyperactivity

SHISong-ying, GUOWei-feng, LIPei-shan, LIUJin-zhan

2017, 33(3): 223-225.

[Abstract](1342) [PDF 1011KB](1870)

Abstract:
Migraine is an independent disease characterized by periodic attacks. As the pathogenesis features， blood deficiency and liver hyperactivity as well as the waxing-waning and transformation of each other exist throughout the whole process of migraine. Therefore， nourishing the blood and calming the liver are put forward as the treatment principle. The key pathogenesis in the attacking stage lies in the wind-yang harassing the upper body， which is mainly treated by dispelling wind and calming the liver， assisted by nourishing the blood and emolliating the liver; With root deficiency and branch excess as the pathogenesis in the intermittent stage， migraine is treated by nourishing the blood and calming the liver， while accompanying syndromes are treated according to their different patterns. The treatment principle of nourishing the blood and calming the liver should be followed strictly.

Discussion on Treatment of Gout Based on Purgation Method

JIAODong-fang, LIUAi-hua

2017, 33(3): 226-227.

[Abstract](5616) [PDF 992KB](3595)

Abstract:
To study the pathogenesis， syndrome differentiation and treatment thoughts of gout based on the purgation method. It is believed that the pathologic evolution of gout can be summarized as five aspects including dampness， heat， phlegm， stasis and deficiency. And phlegm and stasis are the key aspects that determine the severity and prognosis of the disease， which are the unavoidable outcomes of the pathological changes of gout. Clearing and purging method is used in the acute stage to clear heat， dispel the heat and dampness and detoxicate the toxin. Acrid opening and bitter downbearing method is applicable to the recurrent stage， to remove phlegm and blood stasis as well as to dredge meridians and collaterals. Tonifying the deficiency and dredging the stagnation are suitable to the remission stage to ascend lucidity and descend turbidity， for the dredging method can be known as tonification.

Clinical Exploration on 128 Operative Cases with Cervical Intraepithelial Neoplasia Due to Damp-heat Pathogens

LU Qi-bin, REN Qing-ling, GUO Hong-yu, CHEN Cong

2017, 33(3): 228-231.

[Abstract](1141) [PDF 1033KB](1423)

Abstract:
OBJECTIVE To explore the pathogenic factors， diagnosis and treatment strategy and preventive actions via performing a retrospective analysis of 128 operative cases with cervical intraepithelial neoplasia(CIN) due to damp-heat pathogen. METHODS Clinical data of the 128 cases hospitalized for CIN surgery due to damp-heat from January， 2007 to July， 2016， including the symptoms， signs， testing indexes， choice of operation， pathological results as well as relevant pathogenic factors， were recorded on the same chart and analyzed. RESULTS The disease was more likely to happen to females in their 30s to 50s， mainly manifested as the abnormal vaginal discharge， postcoital bleeding， menstrual disorder， accounting for 75%. It is generally classified as the type of damp-heat， accompanied with kidney deficiency， spleen deficiency， liver stagnation and blood stasis after syndrome differentiation in traditional Chinese medicine. 77.3% of the patients experienced cervical hypertrophy together with cervical erosion according to the tests， while still 10.2% of them had no abnormal appearance. The positive result of human papilloma virus (HPV) was mainly seen as 16 / 18， while its quantitative value had little to do with the with the severity of disease. 50.8% of the patients got the same pathogenic results before and after surgery， 29.7% experienced an exacerbation after operation and 19.5% experienced an improvement. The discovery rate of carcinoma in situ or early invasive carcinoma after surgery went up by 5.4% than before. CONCLUSION CIN due to damp-heat has certain clinical specificities， whose occurrence is correlated to HPV infection arising from decreased immune function after premature sexual life， multiple sexual partners and chronic inflammation of cervix and vagina. Cervical cold knife conization has advantages in avoiding the misdiagnosis， missed diagnosis， postoperative massive hemorrhage and interferences of pathologic diagnosis from operative thermoelectric effects. It is therefore vital to recognize the special nature of the disease， choose the proper operation and pay attention to holistic therapy to avoid the reoccurrence.

Clinical Research on Methods of Tonifying qi， Dispelling Dampness and Removing Toxin in Treating Cervical HPV Infection

SUN Hong, ZHU Qin-xian, GU Bo-lin

2017, 33(3): 232-234.

[Abstract](1723) [PDF 1019KB](2807)

Abstract:

Clinical Research on Compound Puchang Yizhi Decoction in Treating Mild Cognitive Impairment After Stroke

YANGLi-jing, TIANJun-biao, LIUXue-fei, XULi-juan, ZHAOCeng-shan, MUPing

2017, 33(3): 235-238.

[Abstract](1272) [PDF 1029KB](1422)

Abstract:
OBJECTIVE To observe the clinical therapeutic effects of the formula capable of resolving turbidity， removing toxin， activating blood circulation and dredging collectrals in improving patients with vascular cognitive impairment of none dementia(VCIND) after stroke. MEHTODS 128 cases with VCIND after stroke were randomly divided into treatment group and control group， with 64 cases in each group. Patients in the treatment group were given compound Changpu Yizhi Decociton warmly both early in the morning and late in the afternoon， one bag per day. While those in the control group were treated with nimodipine， 30mg at a time， three times per day. Both groups received three-month treatment. TCM syndromes， cognitive ability， activity of daily living before and after treatment were evaluated and the levels of homocysteine and acetylcholinesterase were tested to detect the safety index and side effects. RESULTS There experienced an evident improvement of the treatment group in TCM syndrome curative effects and scores， together with obvious increases in the scores of mini-mental state examination(MMSE) and Activity of Daily Living (ADL)， decreased scores in ADAS-cog and declined levels in homocysteine and acetylcholinesterase when compared to the control group. The difference was statistically significant(P<0.05). Neither evident abnormalities nor side effects were detected before and after treatment in the test. CONCLUSION Compound Changpu Yizhi Decoction can efficiently improve the TCM syndromes， cognitive function and daily living activity and reduce the levels of homocysteine and acetylcholinesterase of patients with VCIND after stroke.

Correlation Analysis of Acute Coronary Syndrome with Coronary Artery Lesion and TCM Syndromes

XUEZheng, YEXi, XIENian-jin

2017, 33(3): 239-241.

[Abstract](1210) [PDF 1008KB](1286)

Abstract:
OBJECTIVE To compare the characteristics of intravascular ultrasound in diverse TCM types of acute coronary syndrome(ACS). METHODS 66 patients diagnosed with ACS via IVUS were divided into group of toxic heat and blood stasis syndrome(NHST group with 36 cases) and the group pf non-toxic heat and blood stasis syndrome(Non-NHST group with 30 cases) based on syndrome differentiation. The indexes such as plaque area in criminal lesions， the area of extra-elasticity membrane(EEM)， plaque burden， the incidence of high risk plaques， the positive or negative remodeling percentage and remodeling index(RI) were measured by IVUS. Analysis was performed by statistical package SPSS11.0， with the data being compared. RESULTS The plaque area in criminal lesions， the area of EEM and plaque burden in NHST group were significantly larger than those in Non-NHST group(P<0.01) respectively. The incidence of high risk plaques and RI in NHST group were markedly higher than those in Non-NHST group(P<0.05) respectively. More positive remodeling was observed in NHST group(P<0.01) while more negative remodeling was seen in Non-NHST group(P<0.01). CONCLUSION The coronary arterial atherosclerotic plaques in patients with NHST maybe more unstable.

Effects of Yixinshu Capsule on PCI Patients' Postoperative Life Quality， Anxiety and Depression

LIChe, JIANGJun

2017, 33(3): 242-244.

[Abstract](1245) [PDF 1018KB](1346)

Abstract:
OBJECTIVE To investigate the postoperative life quality， anxiety and depression conditions of the patients with percutaneous coronary intervention (PCI) as well as its efficacy and safety. METHODS Fifty-nine cases with dual anxiety and depression were selected based on Zung self-rating anxiety scale(SAS) and self-rating depression scale(SDS)， and they were randomly divided into the treatment group (40 cases) and the control group (19 cases) according to the ratio of 2∶1. Both groups were given CHD conventional secondary prevention treatment， with Yixinshu capsule being added to the treatment group. Hamilton Anxiety scale(HRSD)， Hamilton depression scale (HAMA) and SF36 scores were performed during the first， second and third month of their hospitalization. RESULTS The anxiety and depression were both improved significantly in two groups. HRSD and HAMA scores before and after treatment were very different from each other(P<0.05~0.01 )， and the treatment group was better than the control group (P<0.05). In the comparison of scores of physiological function， social function， physical limitations and vitality， physical pain and general health of SF36 before and after treatment， the treatment group was better than the control group (P<0.05). CONCLUSION Yixinshu capsule can relieve patients' anxiety and depression after PCI intervention and improve their life quality， without causing severe adverse events.

Clinical Study of Modified Wuling Powder in the Treatment of CSC

XU Hui-lei, ZHONG Min-yi, ZHANG Qi, ZHANG Ya-qin

2017, 33(3): 245-247.

[Abstract](1192) [PDF 1004KB](1310)

Abstract:
OBJECTIVE To observe the clinical effect of modified Wuling Powder in the treatment of central serous chorioretinopathy(CSC) according to syndrome differentiation. METHODS Sixty patients with CSC were randomly divided into the treatment group and the control group， 30 cases in each group. The treatment group was intervened by orally taking modified Wuling Powder， and the control group was treated with orally taking vitamin B1 tablets and vitamin C tablets， with one course lasting for one month. Clinical effects were observed after 3 courses of treatment. RESULTS The total effective rate in the treatment group was 96.7%， and 73. 3% in the control group. The curative effect was better in the treatment group than that of the control group(P<0.05). Vision， FFA fluorescence leakage area and edema degree were compared before and after treatment to be significantly different in two groups(P<0.01). The treatment group was better than the control group(P<0.05). CONCLUSION Modified Wuling Powder has good curative effects in the treatment of CSC.

Clinical Effects Observation of Combined Acupuncture and Thunder-fire Moxibustion in the Treatment of Female Patients with Chloasma

YINLin, GUOHui, LIWen-lin, CHENDi-ping

2017, 33(3): 248-251.

[Abstract](1243) [PDF 1032KB](1354)

Abstract:
OBJECTIVE To observe the clinical effects of the combined acupuncture and thunder-fire moxibustion in the treatment of female patient with chloasma. METHODS Seventy-eight female patients with chloasma were randomly divided into the treatment group and the control group， 9 cases in each group. The treatment group was treated with the combined acupuncture and thunder-fire moxibustion， and the control group was treated with orally taking VitC and VitE， 12 weeks as a course. Indicator changes such as skin lesion area， skin color， estrogen estradiol (E₂) and progesterone (P) were observed in two groups before and after treatment. RESULTS The total effective rate of the treatment group and the control group was 82.1% and 61.5% respectively after the treatment， and the therapeutic effect of the treatment group was better than that of the control group(P<0.05). Skin lesions improvement in the treatment group was better than that of the control group(P<0.05). E₂ levels decreased in two groups and P levels in two groups increased(P<0.05~0.01)， and the treatment group was better than the control group(P<0.05). Dermatology life quality index improvement in the treatment group was significantly better than that of the control group(P<0.05). CONCLUSION The combined acupuncture and thunder-fire moxibustion treatment has better curative effects than western medicine treatment group， and it can regulate E₂ and P levels， which deserves wild application in clinical practice.

The Hypoglycemic Effect of Yin Sang Granules on db/db Mice

ZHANGWen-ying, YANGXiao-lin, WANGXin-zhi, LIURui, DUJun-chao, ZHANGQian, WUHao, BIANHui-min

2017, 33(3): 252-257.

[Abstract](1410) [PDF 1511KB](1427)

Abstract:
OBJECTIVE To investigate the hypoglycemic effect of Yin Sang Granules (YSG) on db/db mice model of spontaneous type 2 diabetes mellitus. METHODS The db/db mice were randomly divided into 5 groups: model group， metformin group， YSG low dosage group， YSG middle dosage group and YSG high dosage group， and these db/m mice were selected as normal group. Fasting blood glucose (FBG) was measured in the first 7 weeks. Oral glucose tolerance tests (OGTT)， insulin tolerance test (ITT) and starch tolerance test (STT) were measured separately at the 9th， 10th and 11th week. The contents of hepatic glycogen， insulin (INS)， glycosylated haemoglobin (GHb)， glycated serum protein (GSP)， total cholesterol (T-CHO)， triglyceride (TG)， high-density lipoprotein cholesterol (HDL-C)， low-density lipoprotein cholesterol (LDL-C) were detected at the 12th week. Pathological change of kidney and pancreas were observed with hematoxylin-eosin staining. RESULTS Compared with the model group， the blood glucose of OGTT， ITT and STT of the YSG groups were significantly decreased (P<0.05~0.01)， and the area under the curve was also decreased (P<0.05~0.01). The hepatic glycogen， HDL-C of the mice in YSG groups were significantly increased (P<0.05~0.01)， and the levels of FBG， GSP， INS， GHb， T-CHO， TG， LDL-C were significantly decreased (P<0.05~0.01). Moreover， the results of pathological section showed that the degree of degeneration of the renal tubular epithelial cells and renal pelvis expansion were reduced， and the margin of islet cells was more regular and its vacuole degeneration was alleviated. CONCLUSION YSG adjusted synthetically the hyperglycemia and dyslipidemia in db/db mice by improving insulin sensitivity.

Comparison of Anti-stress and Immunomodulatory Activities Between Ginseng and its Sulfur-Fumigated Sample

HU Jia-wei, ZHOU Shan-shan, MENG-Xian, WU-Zhen hui, SHEN Ming-qin, LI Song-lin

2017, 33(3): 258-262.

[Abstract](1317) [PDF 1901KB](1637)

Abstract:
OBJECTIVE To study the anti-stress and immunomodulatory activities of ginseng and its sulfur-fumigated sample. METHODS For anti-stress study， bearing hypoxia at normal pressure and load swimming tests were conducted， while for immunomodulatory activity study， cyclophosphamide (CTX) induced immunedeficient mouse model was used， and the index of spleen and thymus， the contents of WBC， CD3*， CD4*， CD8*， CD19* in blood and IL-2，IL-6， IL-8，IFN-γin serum were detected. RESULTS The hypoxia tolerance time， the index of spleen and thymus， and the contents of WBC， IFN-γ， CD4*and CD19*of mice administered with sulfur-fumigated ginseng at low dosage significantly decreased when compared with that administered with non-fumigated ginseng. In addition， at high dosage， the load swimming time， the index of spleen and thymus， and the contents of WBC， IL-2， IL-6， IFN-γ， CD3*， CD4*， CD8*， CD19* of mice administered with sulfur-fumigated ginseng were also decreased significantly when compared with that of non-fumigated ginseng. CONCLUSION All these results suggested that sulfur-fumigation could significantly affect the pharmacological activities of ginseng.

The Liver Toxicity and Mechanism of Psoralen Based on Zebrafish

WUHao, SONGJie, ZHONGQing-xiang, WEIYing-jie, FENGLiang, WANGJing, JIAXiao-bin

2017, 33(3): 263-267.

[Abstract](1315) [PDF 2415KB](1662)

Abstract:
OBJECTIVE Using zebrafish model to explore the toxic of Psoralen on liver METHODS Recording the number of the survival and the death of zebrafish larval in different concertrations of ethanol extract and water extract， to calculate the mortality and evaluate their toxicity. Adult zebrafish were sacrificed after 24h of treatment and remove the liver， Ultrasonic homogenization treatment of zebrafish larval and collect the tissue fluid to detect the ALT， AST and LDH. HE staining was used to observe the change of liver. Meanswhile qPCR was used to detect the mRNA expressions of SREBP-1c，FAS，ACC1 and MTP， At last， Western Blot assay was used to detect the protein expression of SREBP-1c，FAS，ACC1 and MTP. RESULTS The result shows that compared with the control group， the treatmeat groups lead zebrafish larval to death obviously. And the LD₅₀ decreased with the increase of the concentration of the extractive， besides， the toxicity of ethanol extract was greater than water extract at the same concentration. The liver had a degree of hepatic steatosis in treatment groups， and the level of ALT， AST and LDH of tissue fluid in treatments were higher than control group. qPCR results showed that the mRNA of SREBP-1c，FAS and ACC1 level were significantly increased while the level of MTP level was decreased after treatmeat， Western Blot results were the same with qPCR. CONCLUSION The experiment shows that at the concentration of 40μg/mL and 80μg/mL ethanol extract of Psoralen and at the concentration of 300μg/mL and 600μg/mL water extract for 48hours will make zebrafish liver bad even to death. And the possible mechanism is related to hepatic steatosis.

Protective Effects and Mechanism of Polysaccharide from Platycodon Grandiflorum on Damage of PC12 Cells Induced by H₂O₂

GU Cheng-yuan, CHEN Qiu-li, LI Hai-tao

2017, 33(3): 268-272.

[Abstract](1429) [PDF 1176KB](1692)

Abstract:
OBJECTIVE To determine the protective effects of Platycodon grandiflorum polysaccharide (PGP) on PC12 cells injured by H₂O₂ and the underlying mechanisms. METHODS The cell viability was analyzed by MTT assay， the model was established by treating PC12 cells with H₂O₂. The levels of LDH， MDA， SOD and GSH-Px were determined by Kits. ROS production was determined by DCFH-DA fluorescence. mRNA and proteins of NOX2， p22* and Rac were analyzed by qPCR and Western Blot methods. RESULTS Compared with the model， treatment with PGP significantly decrease of LDH， MDA and intracellular ROS production， as well as increase activity of SOD and GSH-Px. Furthermore， PGP could down-regulate mRNA and proteins of NOX2， p22* and Rac. CONCLUSION PGP exhibited protective effect on PC12 cells injury induced by H₂O₂， it may via the suppression of NOX2 activation.

The Effects of Jiawei Fushen Tang on Renal Lesion in Diabetic Nephropathy Rats

CHENWen-lin, LIUHua-dong, LIXuan, ZHANGWei-hua, AIQian-ming, LUOBin, FENGMing

2017, 33(3): 273-278.

[Abstract](1205) [PDF 1420KB](1312)

Abstract:
OBJECTIVE To observe the effects of Jiawei Fushen Tang on diabetic nephropathy rats with renal lesions and discuss the possible mechanism. METHODS 40 male SD rats were treated with intraperitoneal injection of STZ+CFA to establish diabetic model after normal feeding for 1 weeks. After successful modeling， the rats were divided into model group (M)， JWFST (L1) group，JWFST (L2) group，JWFST(L3) group. L1， L2， L3 group were treated with JWFST 8g/(kg·d)，16g/(kg·d)， 32g/(kg·d); the model group was given normal saline; the normal control group (C) was 8 male SD rats with conventional feeding. The blood sugar on a regular basis for 8 weeks were recorded. After the experiment， collect 24h urine， the serum was collected and biochemical parameters， like SCr， BUN， SOD， MDA， UPwere detected. Then the kidneys were harvested and the expressions of nephrin and podocin in the kidney were detected by IF method and qPCR method. And then observed the renal lesions under light microscope. RESULTS Compared with the model group， the INS， SCr， BUN， MDA， UP of treatment groups were significantly decreased， and SOD was increased significantly. The expressions of nephrin and podocin were raised significantly by JiaWeiFuShenTang. CONCLUSION JiaWeiFuShenTang can significantly improve the renal damage of DN rats， and delay the development of diabetic nephropathy.

Effect of Combination of Glycyrrhizae Radix et Rhizoma and Euphorbia Pekinensis Radix in Resisting Hepatocellular Carcinoma ascites in Mice

YANChen, LIYu-ting, GUOXiao-dong, GUOQiu-yan, ZHANGYan-qiong, LINNa

2017, 33(3): 279-283.

[Abstract](1358) [PDF 1104KB](1455)

Abstract:
OBJECTIVE To investigate the effect of the combined administration of herbal pair Euphorbia Pekinensis (DJ) and Glycyrrhiza glabra (GC) at different doses or ratios on hepatocellular carcinoma (HCC) ascites in mice. METHODS A total of 120 male Kunming mice were randomly divided into normal control， model， DJ-GC groups [ratios 1∶0.5， 1∶1， 1∶2， 1∶4]， DJ-alone group and GC-alone group. HCC ascites model was constructed by inoculating H22 cells intraperitoneally. HCC ascites mice in the drug treatment groups were intragastrically administrated with corresponding doses of Euphorbia Pekinensis and Glycyrrhiza glabra for 12d， respectively. Then， the therapeutic effects of herbal pair DJ and GC acting on HCC acites was analyzed in terms of body weight， ascites volume， abdominal circumference and the index of liver and kidney. After that， the expressions of AQP2 and V2R were detected by Western Blot， and the level of ALT， AST， BUN， CR were detected by biochemical method. RESULTS When compared with model group， the DJ-alone group and DJ-GC 1∶2 group could efficiently reduced the ascites volume， abdominal， body weight (P<0.05~0.01) and the expression of aquaporin-2 and renal vasopressin receptors-2 in the kidney tissues (P<0.05~0.01). DJ-GC 1∶2 group could enhance the effect of DJ-alone group ( P<0.05~0.01 )， while the DJ-GC 1∶4 group could reduce the effect of DJ-alone group ( P<0.05~0.01 ). CONCLUSION The therapeutic effects of DJ on HCC were altered in accordance with its combination with GC at different ratios by being enhanced or weakened， which might be partially related to their regulation on aquaporin-2 and renal vasopressin receptors-2 signal axis.

Regulatory Effect of JianpiBushen Recipe on the Secretion Function and Expressions of NF-κB and MMP-9 in Placental Tissues of Pregnant Rats with Subclinical Hypothyroidism

ZHANGYin, XUXiao-feng

2017, 33(3): 284-288.

[Abstract](1210) [PDF 1460KB](1367)

Abstract:
OBJECTIVE To study the effects of JianpiBushen recipe(JBR) on the secretion function and Nuclear Kappa B(NF-κB) and matrix metalloproteinase-9（MMP-9）in placental tissues of pregnant rats with subclinical hypothyroidism(SCH) and investigate the protective mechanism of JBR. METHODS Ninety female adult Wistar rats were randomly divided into 2 groups: normal control group(false thyroid surgery was carried out， n=10) and model control group(total thyroidectomy was carried out， n=80).The animal model of SCH was established by using＂total removal of the rats thyroid 30 days， levothyroxine was given to the rats by subcutaneous injection at 9.5μg/(kg·d)＂， All rats in the model with overt hypothyroidism were selected 10 as low dose Chinese medicine before pregnant group(levothyroxine was given to the rats by subcutaneous injection at 9.5μg/kg·d and low dose Chinese medicine， Q)， others were injected levothyroxine subcutaneously at 9.5μg/(kg·d) for 9 days， serum TSH and TT4 were detected by radioimmunoassay on the tenth day and screening of SCH rats， the ratio of male to female=2∶1 was used as a cage. Through the observation of vaginal suppository and vaginal smear to find sperm to determine whether the mating and determine D0 day. Then randomly divided into 4 groups: ①The control group is SCH group(C) ②Low dose Chinese medicine group(L) ③High dose Chinese medicine group(H) ④Western medicine group(X). Serum chorionic gonadotropin (HCG)， estradiol (E₂) and progesterone (P) of the pregnant rats with SCH on the 10*day after conception (D10) in each group were detected by radioimmunoassay； The expressions of NF-κB and MMP-9 in placental tissues of pregnant rats with SCH on D10 in each group were detected by immunohistochemistry and Westernblottingassay. RESULTS Compared with the C group， on the tenth day， the serum levels of P and HCG in each treatment were all significantly higher (P<0.01)， the serum levels of E₂ were significantly higher in the X group(P<0.05)， The results of immunohistochemistry and Western Blot showed that the expressions of NF-κB and MMP-9 in placental tissues in each treatment group were all significantly higher (P<0.05). CONCLUSION JBR can promote the secretion of placenta and increase the expressions of NF-κB and MMP-9 in the placenta of pregnant rats with SCH.

Effects of Qingtouxiere Decoction on Expression of TLR-7， MyD88 and NF-κB mRNA and Protein in Ana-1 Cells of Influenza A H1N1 Influenza Virus

FENGLi-qian, YANGLi-li, LIMing-zhen, SUNYu, LIUTao

2017, 33(3): 289-294.

[Abstract](1333) [PDF 1117KB](1494)

Abstract:
OBJECTIVE To observe the effect mechanism of Qingtouxiere Decoction on anti-influenza A H1N1 influenza virus in vitro. METHODS The macrophages were stimulated with FM1 in the lungs of influenza A virus， respectively， with different concentrations of 0.388mg/mL， 0.194mg/mL， 0.097mg/mL， 0.0485mg/mL and 0.02425mg/mL. The expression of TNF-α， IP-10 and IL-6， TLR-7， mRNA and protein expression of MyD88 and NF-κB. RESULTS The expression of TNF-α， IP-10 and IL-6 in macrophages of Ana-1 mice was inhibited by Qingtouxiere Decoction， and the infection of H1N1 virus was decreased in 0.02425mg/mL group (P<0.01). The level of TNF-α was significantly lower in the group of Qingtouxiere Decoction 0.097mg/mL (P <0.01). The expression of TLR-7， MyD88 and NF-κB mRNA and protein were increased after Ana-1 macrophage infection in H1N1 influenza virus group. The expression of TLR-7， MyD88 and NF-κB mRNA was increased by 0.0485mg/mL group (P<0.01). The expression of MyD88 mRNA in the H1N1 infected group was significantly higher than that in the control group (P <0.01). The expression of TLR-7 mRNA was significantly inhibited The expression of NF-κB mRNA in H1N1 virus group was significantly higher than that in H1N1 virus group (P<0.01). The expression of TLR-7 protein in H1N1 virus group was significantly higher than that in H1N1 virus group(P<0.01). The expression of NF-κB in the H1N1 infected group was significantly higher than that in the H1N1 virus group (P<0.01). The expression of NF-κB in the H1N1 infected group was significantly higher than that in the control group (P<0.01). CONCLUSION The activation of TLR-7/MyD88/NF-κB signaling pathway induced by H1N1 influenza virus can inhibit the expression of downstream inflammatory factors and play the antiviral effect.

Metabolomic Approach to Evaluating Fetal Toxicity of Pinellia Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumina Based on BeWo in vitro Placenta Model

SHENShi-fu, SHANJin-jun, XIEHui-hui, XUJian-ya, WANGXiao-rong, DILiu-qing

2017, 33(3): 295-300.

[Abstract](1557) [PDF 1891KB](1485)

Abstract:
OBJECTIVE To evaluate the potential developmental toxicity of Pinellia Rhizoma(BX) and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumina(JBX) by analyzing the changes of intracellular metabolites after the intervention of BX and JBX by gas chromatography-mass spectrometry (GC-MS). METHODS The BeWo cell line derived from placenta tissue was used to construct the model of the in vitro placenta， and the changes of the metabolites and their relative contents in the cells were detected by GC-MS after the intervention of BX and JBX. RESULTS The nine metabolites of glycine， alanine， proline， glucose， galactose， stearic acid， inositol and so on were identified. CONCLUSION GC-MS technology is feasible for evaluation of developmental toxicity of BX and JBX.

Qualitative Investigation on Changes of Chemical Constituents from Volatile Oils Between Crude and Vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium Herb-pair Based on AMDIS and Retention Index Coupled with GC-MS Analysis

FANKai-lei, CAIHao, DUANYu, PEIKe, WANGXiao-qi, XUYang-yang, ZHAOJia-yu, ZHOUJia

2017, 33(3): 301-307.

[Abstract](1565) [PDF 3046KB](1354)

Abstract:
OBJECTIVE The effects of vinegar-processing on overall compatibility of Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were investigated based on assessment model of regulation on painful menstruation as efficacy from overall angle of the herb-pair. METHODS The experimental strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index was used to analyze the changes of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively and systematically. RESULTS Fifty-four chemical components had been identified in volatile oils of crude Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair while fifty-two chemical components had been identified in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair. In addition， The changes of chemical constituents from volatile oils in crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were inconsistent with those in crude and vinegar-processed Cyperi Rhizoma (Artemisiae Argyi Folium). On the whole， in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair， the content of most low boiling point compounds belonging to Artemisiae Argyi Folium was decreased while the content of most high boiling point compounds belonging to Cyperi Rhizoma had no obvious change， and the relative content of α-cyperone was increased to some extent. The compounds with significantly increasing contents in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were deoxy-sesquiterpenoids， long chain fatty alcohols， and long chain aliphatic ketones. Nevertheless the content of terpenoid oxide derivatives was decreased. CONCLUSION By employing the strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index， we could identify the differences of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively， scientifically， and rapidly. Overall， the toxicity of volatile oils in Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair was decreased， the safety was increased， and the efficacy of regulation on painful menstruation was strengthened after vinegar-processing. The strategy used in this paper would be a certain reference significance for analyzing other complex systems on Chinese herbal medicines.

Preparationof Doxorubicin-Copper-Curcumin Nano-micelles and its In-vitro Activities Evaluaion

CHENJing, QIAOHong-zhi, CHAIYa-ping, DAIXin-yi, SUNJuan, DINGQing-long, DILiu-qing

2017, 33(3): 308-312.

[Abstract](1381) [PDF 1379KB](1705)

Abstract:
OBJECTIVE With Poloxamer 407(P407) as the carrier， doxorubicin-copper-curcumin ternary complex was encapsulated in the micelles to achieve quantitative and intelligent drug release behavior and synergistic effect. METHODS With P407 as the carrier，the preparing technology was optimized by Box-Behnken design and the in vitro pH-sensitive drug release behavior of DOX-Cu-Cur/P407 was examined; the MTT analysis was carried out to evaluate the cytotoxicity to tumor cells. RESULTS Under the optimum condition， the encapsulation efficiency of doxorubicin and curcumin was (93.17 ± 1.05)% and (100.03 ± 1.10)%， size distribution was (31.06 ± 4.20) nm，PDI is 0.174 ± 0.028. Meantime， in vitro drug release test showed the DOX-Cu-Cur/P407 micelles was pH-sensitive. The MTT analysis showed DOX-Cu-Cur/P407 micelles could significantly inhibit the tumor cells with a better synergistic effect compared with free drugs. CONCLUSION The optimal DOX-Cu-Cur/P407 micelle is uniform in size and has satisfactory encapsulation efficiency. Meanwhile it is sensitive to acidic microenvironment of tumor and can achieve the purpose of quantitative and intelligent drug release.

Study on Preparation and Evaluation of Andrographolide Nanosuspensions

WANGJing-xian, LIWen, CHENTing, LIJun-song

2017, 33(3): 313-316.

[Abstract](1368) [PDF 1267KB](1496)

Abstract:
OBJECTIVE To established the andrographolide nanosuspensions (ADG-NS) with improved its dissolution and membrane permeability. METHODS ADG-NS were prepared by wet media milling technique. The quality of the nanosuspensions was characterized including the particle size and distribution， Zeta potential， particle morphology and crystal physical state. In vitro dissolution test and Caco-2 permeability test were used to preliminarily evaluate the ADG-NS. RESULTS The particle size， polydispersity index (PDI) and Zeta potential of ADG-NS were (215.6 ± 3.3) nm， 0.165 ± 0.020 and (-36.9 ± 2.8) mV， respectively. SEM analysis showed that there was no adhesion and aggregation between particles， the particles were rod or block， and the size distribution was uniform. DSC and XRPD results showed that the nanoparticles were present in the amorphous form. The dissolution rate of ADG-NS were significantly enhanced in different pH buffer solutions compared to ADG and commercial dripping pills. Caco-2 permeability test revealed that ADG-NS showed a significant increase in the membrane permeability (P_app) compared to the group of ADG (P<0.01). CONCLUSION The ADG-NS prepared by wet media milling technique can improve the in vitro dissolution rate and membrane permeability of andrographolide (ADG).

Simultaneous Determination of 7 Active Components in Qianyang Yuyin Prescription by UPLC

MENGYan, HANLin-ran, XUJian-jun, LIUYa-lin, FANGZhu-yuan, HUANGXi

2017, 33(3): 317-319.

[Abstract](1203) [PDF 1066KB](1603)

Abstract:
OBJECTIVE An UPLC method in vitro was established for simultaneous determination of seven active components in Qianyang Yuyin prescription， including gallic acid， protocatechuic acid， loganin， stilbene glucoside， cyasterone， cinnamic acid and harpagoside. METHODS The analysis was performed on an Waters H-Class UPLC C18 column (2.1 mm×100 mm， 1.7 μm) eluted with a gradient mobile phase of acetonitrile-0.1% formic acid solution system at a flow rate of 0.3 mL/min. The detective wavelength was set at 250nm， the column temperature was maintained at 30℃ and the injection volume was 2 μL. The 7 main chemical compounds in Qianyang Yuyin prescription were determined by UPLC. RESULTS The linear relationship of the 7 chemical constituents in Qianyang Yuyin Prescription were good in their respective concentration ranges(r>0.997 5)， The average recoveries were 95.8%~103.75%， the RSD of average recovery were 0.1%~1.0%， the RSD of precision were 0.10%~0.56%， the RSD of stability were 1.25%~9.04% and the RSD of reproducibility were 1.01%~3.24%. CONCLUSION The new established method in Qianyang Yuyin prescription was simple， accurate and reliable， which will supply instruction and reference to the internal Pharmacokinetics of Qianyang Yuyin prescription in the late works of this issue.

Differentiation and Treatment of Coronary Heart Disease on the Basis of Compound Pathogenesis by Professor ZHOU Zhongying

LI Rui-min, LIANG Qiu-yu, YE Fang

2017, 33(3): 320-322.

[Abstract](1205) [PDF 1014KB](1641)

Abstract:
Through the research and analysis of Chinese medicine master Zhou Zhongying's records of the differentiation and treatment of coronary heart disease， it is summarized that the syndrome differentiation of coronary heart disease by professor Zhou is established on compound pathogenesis and the treatment with a view to composite prescription. By listing common compound pathogenesis of the differentiation and treatment of coronary heart disease by professor Zhou， evidence can be given that the differentiation and treatment of coronary disease is aimed at the pathogenesis including deficiency of qi and yin， stagnation of heart ying， failure in spread of chest yang， retention of phlegm and stasis， etc.

Clinical Experience of National TCM Master Xuan Guowei in Treating Pemphigus by Stages

XIETing, JINGFang-yi, JIAShu-lin, ZHUPei-cheng, LIUChan-ke, LIHong-yi

2017, 33(3): 323-324.

[Abstract](1802) [PDF 990KB](1695)

Abstract:
This paper collected and introduced the clinical experiences of national TCM master Xuan Guowei in treating pemphigus by stages. Xuan Guowei argued that in the acute stage， the main syndrome was intense heat toxin and mainly treated by resolving toxins and dispelling pathogen. With the development of the disease course， warm-febrile pathogen would change into dryness， burning fluid and consuming qi and thus in the later stage， yin deficiency accompanied with dampness-heat was mostly seen. It should be treated by dispelling dampness and fortifying the spleen， as well as clearing heat and nourishing yin. The disease has a relapsing course and could be easily conversed into the impairment of yin and deficiency of qi. Xuan advocated the combination of Chinese and western medicine for the treatment of pemphigus. On the basis of hormone therapy， the external treatment of Chinese medicine according to different stages of the disease could further be added.

2017 Vol. 33, No. 3

ArchiveMore+

Author field More+

Edit fieldMore+