南京中医药大学学报

Introduction to Diagnosis and Treatment of Vertigo in Internal Canon of Yellow Emperor

TIANYong-yan, HURong, ZHAOXiao-qiang

2017, 33(2): 109-111.

[Abstract](1571) [PDF 341KB](2402)

Abstract:
It is believed in Internal Canon of Yellow Emperor that the reasons and pathogenesis of vertigo involve invasion of exogenous factors， changes of qi circulation， internal damage of visceral organs， abnormal circulation of meridian qi. In terms of meridians， it is related to the Urinary Bladder Channel of Foot Taiyang， Liver Channel of Foot-Jueyin and Gall-bladder Channel of Foot-Shaoyang. As for the internal organs， it is correlated to the brain and liver. Acupuncture is put forward as the major method for vertigo in Internal Canon of Yellow Emperor， with the treatment principles being asthenia syndrome requiring purgation and deficiency syndrome demanding tonification.

Treatment of Cough Variant Asthma From Wind-phlegm

CHEN Yan, ZHU Jia

2017, 33(2): 112-114.

[Abstract](1268) [PDF 352KB](1526)

Abstract:
ABSTRACT From the inside and outside pathogenic wind in the treatment of cough variant asthma， it is believed that the basic pathogenesis is lung deficiency accompanied with wind pathogen invasion， with wind being the main cause. During the acute stage， it is focused on dispelling exogenous pathogen， relieving internal wind and removing phlegm. While at the remission stage， it is oriented in the regulation of zang and fu to remove the internal wind. At the normal situation， it is recommended to regulate the constitution and enhance the healthy qi.

Compatible Principle of Prescriptions for Treating Cough and Asthma in Prescriptions for Universal Relief

CHENXu, DAIShen, WANGPeng-li, FANYu-hao

2017, 33(2): 115-117.

[Abstract](1191) [PDF 323KB](1108)

Abstract:
OBJECTIVE To analyze the compatible principle of lung system prescriptions for treating cough， pant-cough and asthma in Prescriptions for Universal Relief. MRTHODS The prescriptions applied for treating cough， pant-cough and asthma in Prescriptions for Universal Relief were collected and classified into cough formula， pant-cough formula and asthma formula. Association rules were used to process and analyze the selected 1 036 formulas， with the core herbs， herbal pairs and herbal groups being obtained. Then their corresponding properties and tastes were further analyzed. RESULTS Among the above three groups， apart from their own unique high frequently used herbs， the herbs like almond， pinellia ternata， aster， flos farfarae， fritillaria， licorice root， whitish honey， fresh ginger， white mulberry root-bark and the fruit of Chinese magnoliavine appeared as the commonly applied herbs with over 10% of support rate. In terms of drug compatibility， such medicine pairs as almond- licorice root-fresh ginger， pinellia ternata-licorice root-fresh ginger and almond- licorice root-ephedra can be seen in each formula. CONCLUSION Prescriptions for Universal Relief focuses much on tonification of healthy qi， invigoration of spleen and removal of dampness in treating cough and pant-cough， and especially on dispersing and descending of lung qi， purging lung and relieving asthma when dealing with pant-cough， while it attaches great importance to pathogen removal and stopping asthma for treating asthma， all of which demonstrates the treatment principle of treating secondary symptoms for emergency.

Influence of Zuogui Pill on Bone Metabolism in Grave's Ophthalmopathy After Methylprednisolone Pulse Therapy

HU Yong-xin, SUN Li, CHU Xiao-qiu, XIE Shao-feng, LIU Chao

2017, 33(2): 118-121.

[Abstract](1540) [PDF 391KB](1265)

Abstract:
OBJECTIVE To explore the influences of Zuogui Pill on such bone metabolism indexes as P1NP， β-CTX， 25OHD and ALP in patients with Grave's ophthalmopathy(GO) after methylprednisolone pulse therapy. METHODS 60 cases with GO due to kidney yin deficiency catering for the condition were selected and into the treatment group(32 cases) and the control group(28 cases). Patients in the treatment group were given methylprednisolone pulse therapy and Zuogui Pill， while those in the control group were treated with methylprednisolone pulse therapy and placebo. The syndrome integral scores and bone biochemical markers of both groups were observed before and after treatment. RESULTS The syndrome integral scores of the treatment group experienced an evident improvement than before(P<0.05). The treatment group is more superior to the control group in improving soreness and weakness of waist and knees， tidal fever and night sweating(P<0.05)， with better therapeutic effects(P<0.01). Both β-CTX and ALP levels in the blood serum went down， with significant differences being noticed in the β-CTX level of both treatment groups(P<0.05). CONCLUSION Chinese medicine Zuogui Pill is able to improve the symptoms caused by kidney yin deficiency， inhibit the osteoclast activity， rectify the high-bone turnover， which is conductive to preventing osteoporosis due to glucocorticoid.

Relationship Between Osteoclast-related Cytokines in Patients with Osteoporosis and TCM Syndromes

WANG Lei, XIE Zhi-hui, YUAN Chun-sheng, GAO Xiang-bin, JIN Lin-fang

2017, 33(2): 122-124.

[Abstract](1384) [PDF 360KB](1169)

Abstract:
OBJECTIVE To explore the correlation between the expression of osteoclast-related cytokines like MCSF， RANKL， TNFα IL1αin patients with osteoporosis and TCM syndromes. METHODS 120 cases with osteoporosis were divided into the kidney yang deficiency group(30 cases)， deficiency of spleen and stomach group(30 cases)， liver-kidney yin deficiency group(30 cases) and qi stagnation and blood stasis group(30 cases) based on syndrome differentiation. Fasting blood samples early in morning were collected and the expression of four items including MCSF， RANKL， TNF and IL1αwere tested by applying euzymelinked immunosorbent assay method. RESULTS Kidney yang deficiency group experienced evident lower expression of MCSF and RANKL than the other three groups， with significantly statistical differences， while no differences were noticed in the expression of TNFαand IL1αamong the four groups. CONCLUSION Detection of MCSF and RANKL expression can be considered as an effective way to distinguish the kidney yang deficiency syndrome from the other three types. Hence， we believe that there exists quantified objective evidence for osteoporosis syndrome differentiation in TCM.

Clinical Research on Qingre Chushi Jiedu Formula in Treating Acne Due to Damp-heat in Spleen and Stomach

LUJing, LIUXiu-min, HANXiao-dong, LIUHong-min

2017, 33(2): 125-128.

[Abstract](1275) [PDF 390KB](1093)

Abstract:
OBJECTIVE To explore the influences of Qingre Chushi Jiedu Formula on skin physiological indexes and immune indexes in patients with acne due to damp-heat in spleen and stomach. METHODS 102 cases with acne caused by dampness-heat in the spleen and stomach hospitalized in our hospital from June， 2015 to September， 2016 were chosen and randomly divided into the observation group and control group based on random number method， with 51 cases in each group. Patients in the control group were given Danshentong Capsule， while those in the observation group were treated with Qingre Chushi Jiedu Formula. The treatment lasted for four weeks in total. Then the improvement of clinical symptoms were observed， with skin physiological indexes(including skin moisture content， skin oil and pH value)， serum interleukin(IL-2， IL-4， IL-10) as well as interferon(INF-γ) being tested before and after treatment to evaluate the clinical therapeutic effects of both groups. RESULTS Scores of lesion extent， lesion amount， lesion color， lesion swelling and pain as well as total lesion scores and TCM symptom scores of both groups went down significantly(P<0.01)， with the observation group being better than the control group(P<0.01). The total therapeutic effect rate of observation group was 90.2%， notably higher than 66.7% of the control group(P<0.01); Heart， liver and kidney function， together with blood routine test and urine routine test of the both groups were within the normal range， with no side effects being noticed. The skin moisture extent of the two groups after treatment went up evidently(P<0.01)， whereas the skin oil went down significantly(P<0.01). The two groups enjoyed evidently increased level of IL-2 and INF-γ after treatment (P<0.01) and decreased level of IL-4 and IL-10(P<0.01). while the recurrence rate of the observation group either four weeks or eight weeks after treatment was much lower than that of the control group(P<0.05). CONCLUSION Qingre Chushi Jiedu Formula can efficiently improve the clinical symptoms of patients with acne due to damp-heat accumulation in spleen and stomach and reduce the recurrence rate， whose mechanism might be related to the improvement of patients' skin physiological indexes and immune function.

Clinical Study of Yishen Sanjie Quyu Decoction in the Treatment of Adenomyosis Caused by Kidney Deficiency and Blood Stasis

WANG Hui-ling, LIU Shan-shan, YANG Jun

2017, 33(2): 129-132.

[Abstract](1140) [PDF 384KB](1077)

Abstract:

Clinical Observation of Duhuo Bugu Decoction Combined with Zoledronic Acid in the Treatment of Malignant Bone Metastasis

BAI Ping, CHEN Jiao-jiao, HU Ling-jing, ZHANG Guo-duo

2017, 33(2): 133-135.

[Abstract](1356) [PDF 355KB](1158)

Abstract:
OBJECTIVE To observe the clinical effects of the combination of Duhuo Bugu decoction and zoledronic acid in the treatment of malignant bone metastasis. METHODS Fifty patients with bone metastases of malignant tumor were selected from the Department of oncology， Chongqing Hospital of traditional Chinese medicine from September 2014 to February 2016， and were randomly divided into the observation group and the control group. Two groups were both treated by zoledronic acid， and the observation group was also treated by adding Duhuo Bugu Decoction. Changes of the analgesic effects， analgesic dosages and life quality scores(KPS) of two groups were observed before and after treatment. Urinary type I collagen amino terminal peptide (uNTX) and bone alkaline phosphatase(BALP) were measured via ELISA method before and after treatment. RESULTS Compared with the control group， the analgesic medicinal amount of the observation group reduced significantly(P<0.05)， and KPS improved significantly(P<0.05). After the treatment， numerical pain scores(NRS) of two groups had no significant changes(P>0.05). After the treatment， uNTX and sBALP level of the treatment group decreased compared with that before treatment (P<0.01). While compared with the control group， the difference was not significant(P>0.05). CONCLUSION The combination of Chinese herbs and zoledronic acid is an effective therapy for bone metastasis malignant tumors. Chinese herbs can effectively improve the analgesic effect， reduce the analgesic medicinal amount， improve the patients' life quality， and have a tendency of synergy in improving the bone destruction caused by tumor.

Clinical Observation of Acupoint Pressing Gymnastics on Primary Hypertension of Abundant Phlegm-dampness Syndrome

ZHANGHui-ling, GUOXiu-jun, ZHANGMin, CAOCong, YUBei

2017, 33(2): 136-139.

[Abstract](1127) [PDF 388KB](1355)

Abstract:

Supplementary Role of Shenqi Fuzheng Injection to Digestive Tract Tumor During Chemotherapy

ZHANGGong-zheng, CHENHong-tao

2017, 33(2): 140-143.

[Abstract](1058) [PDF 363KB](1078)

Abstract:
OBJECTIVE To explore the clinical supplementary role of Shenqi Fuzheng Injection in treating digestive tract tumor during chemotherapy and its resistance to the side effects induced by chemotherapy. METHODS 156 cases with digestive tract tumor hospitalized in our hospital from 2014 to 2016 were collected and randomly divided into the combined chemotherapy group and control group， with 78 cases in each group. The conventional chemotherapy regimen was designed to the control group， while patients in the treatment group were further given 250mL/d of Shenqi Fuzheng Injection. Then chemotherapeutic effects were assessed after chemotherapy to evaluate the chemotherapy efficiency rate and disease control rates(DCR). To be specific， the activities of CD3⁺， CD4⁺， CD8⁺， CD4⁺/CD8⁺ and NK cells as well as the peripheral blood cell level before and after treatment were tested to record and count the side effects appearing during chemotherapy. RESULTS There existed statistical differences between the combined chemotherapy group and control group in efficiency distribution after chemotherapy(P<0.05). The blood cell level of both groups went down after chemotherapy. The immune cell activity of the control group went down， while that of the combined chemotherapy group went up. And the levels of CD3⁺， CD4⁺， CD8⁺， CD4⁺ /CD8⁺， NK and diverse blood cells of the combined chemotherapy group were higher then those of the control group(P<0.05); The appearance rate of the side effects in the combined chemotherapy group was 74.35%， lower than 88.46% of the control group(P<0.05). CONCLUSION Shenqi Fuzheng Injection has certain effects on the improvement of chemotherapy efficiency for patients with digestive tract tumor， and also change the hypoimmunity and side effects induced by chemotherapy.

Biotransformation of Betulinic Acid From Nelumbinis Rhizoma and Cytotoxicity Determination of the Fermentation Products Against Cancer Cell Lines

LI Hui-wei, DUAN Jin-Ao, LIU Pei, ZHANG Sen, JIANG Shu, TAO Wei-wei

2017, 33(2): 144-149.

[Abstract](1408) [PDF 779KB](1086)

Abstract:
OBJECTIVE Betulinic acid (BA) from the waste of Nelumbinis Rhizoma (NR) was introduce sugar groups at specific positions by biotransformation. The fermentation products were isolated and purified. Their structural characterization and antitumor activities were also investigated， which may improve utilization of NR. METHODS The content levels of BA in different parts of NR were analyzed by the modified HPLC-PDA method. With the transformation ability of strains screened， the transformation products were analyzed using a UPLC-QTOF-MS method， isolated and purified by silica gel column chromatography repeatedly. The structure characteristics were analyzed by NMR and MS. Cell viability was evaluated by the methylthiazolyl tetrazolium (MTT) assay. RESULTS The content of BA(1) in the nodus was measured to be 0.621%， the highest in the parts of NR. The strain， identified as Bacillus amyloliquefaciens FJ18， was used in the biotransformation. A novel glycosylation derivative of betulinic acid (BA) derivative， 3β-hydroxy-lup-20(29)-en-28-oic acid 28-O-(4-oxy-4-oxobutanoic acid-4-O-β-D-glucopyranosyl) ester， together with a known derivative， 28-O-β-D-glucopyranosyl-3β-hydroxy-lup-20(29)-en-28-oate were obtained by bioconversion of BA. Their structures were determined on the basis of spectroscopic data. The biotransformation products showed in vitro cytotoxic activity against A375 (melanoma)， HeLa (cervical carcinoma)， U251 (glioma)， SH-SY5Y (neuroblastoma) and MCF-7 (breast cancer) cell lines. CONCLUSION The biotransformation of betulinic acid to the derivatives featuring D- glucopyranosyl moieties was accomplished. The in vitro cytotoxicity study revealed that the derivatives exhibit potent cytotoxic activity against cancer cell lines.

Influence of Huoxue Tongfu Formula on Expression of Nrf2， HO-1， NQO1 and mRNA in Rats with Postoperative Peritoneal Adhesion

YANGLi-li, ZENGLi, LIWen-lin, LIANZi-yu, GAOHai-ting, XUEKang-li

2017, 33(2): 150-154.

[Abstract](1182) [PDF 655KB](1060)

Abstract:
OBJECTIVE To investigate the effect of Huoxue Tongfu Formula on the expression of Nrf2， HO-1， NQO1 and mRNA in rats with postoperative peritoneal adhesion. METHODS Peritoneal adhesion was evaluated by gross abdominal adhesion score.The expressions of Nrf2， HO-1 and NQO1 were detected by western blot method. The expressions of Nrf2、HO-1 and NQO1 mRNA were tested via qPCR. RESULTS Huoxue Tongfu Formula could significantly reduce adhesion scores (P<0.01)， manifested by evidently increased expression of Nrf2， HO-1 and NQO1 protein and mRNA(P<0.01). CONCLUSION The expression of Nrf2， HO-1 and NQO1 in the adhesions of rats with peritoneal adhesion is significantly affected by Huoxue Tongfu Formula， which is able to resist peritoneal adhesion by regulating Nrf2 and its downstream factors and enhancing the ability of anti-oxidative stress.

Establishment of Mice Model of COPD with Depression and Intervention Effects of Modified Wendan Decoction

TAOWei-wei, HUANGXiao-yan, SHENJia-li, XIAOQing-ling, XUEWen-da, CHENCan-ruo, XIABao-mei, WUHao-xin, CHENGang

2017, 33(2): 155-159.

[Abstract](1570) [PDF 814KB](1263)

Abstract:
OBJECTIVE To establish the mice model of chronic obstructive pulmonary disease (COPD) complicated with depression induced by smoke， and to evaluate the therapeutic effects and mechanism of modified Wendan Decoction on these mice models. METHODS Mice were randomly divided into normal control group and model group. Model group was given smoke four times a day. After 15 weeks， sugar consumption of both the model group and the control group were tested as well as the pathological changes of airway and lung tissue of the two groups were observed. In recognition of COPD with depression models being successfully constructed， mice in the model group were further randomly divided into model group， high dose group of Modified Wendan Decoction， low dose group of Modified Wendan Decoction and positive drug group， with the medicine administration lasting for 3 weeks. Then the sugar consumption， tail suspension test， pulmonary function and the number of inflammatory cells in bronchoalveolar lavage fluid (BALF) of each group were tested and the airway and lung tissue pathological changes were once more observed. RESULTS Compared with normal control group， the weight and sugar consumption of model group decreased and the enhanced respiratory pause (Penh) increased after 15 weeks of smoking. Compared with the model group， the body weight and sugar consumption of the Modified Wendan Decoction group increased， with significantly reduced inspiratory resistance (RI)， increased lung dynamic compliance (Cdyn)， decreased total number of leukocyte and neutrophils than those of the model group. In addition， the bronchoalveolar lavage fluid and inflammatory factors in hippocampus were decreased. CONCLUSION The COPD with depression mouse model established by smoke is in accordance with the disease development and and its clinical features. Chinese medicine Modified Wendan Decoction has therapeutic effects on the improvement of pathological and functional changes in mice with COPD and depression.

Effect of Huangqi Baoxin Decoction on Ventricular Remodeling in Rats with Dilated Cardiomyopathy Induced by Adriamycin

LULei, ZHANGPei-ying, CHENYong-gang, LIUXiao-dan

2017, 33(2): 160-166.

[Abstract](1422) [PDF 1465KB](1194)

Abstract:
OBJECTIVE To preliminary discuss the influence and mechanism of Huangqi Baoxin Decoction on ventricular remodeling in rats with dilated cardiomyopathy (DCM) induced by adriamycin. METHODS 90 SD rats were given intraperitoneal injection of adriamycin to establish the DCM model， with 55 rats model being succeed. Then they were randomly divided into model group (the same volume of physiological saline solution)， low-dose group with Huangqibaoxin Decoction (5.67 g/kg)， middle-dose group with Huangqibaoxin Decoction (11.34 g/kg)， high-dose group with Huangqibaoxin Decoction (22.68 g/kg)， and captopril group (6.75 mg/kg of captopril)， with 11 rats in each group. The rats were given orally administrated drugs once a day， with 10 rats in the normal control group being treated with the same volume of physiological saline solution. 4 weeks later， rats in each group went through the echocardiography to access the construction and function of their left ventricular. Crotid artery intubation was applied to monitor their hemodynamic indexes， with changes of myocardial tissue morphology being observed. Besides， NT-proBNP， ST2， AngⅡ， ALD and Renin were tested through ELISA， together with the activity of SOD， MDA， and LDH being tested via colorimetric method. RESULTS Compared with the normal group， the LVEDD and LVESD in rats of the model group were increased， with EF and FS being decreased (P<0.01). The LVDP，±dp/dtmax went down， while the LVEDP went up(P<0.01).The heart weight index increased(P<0.01)， with the myocardial cells being arranged in disorder and massive hyperplasia of fibrous tissue. The NT-proBNP， ST2， Renin， AngⅡ and ALD level increased(P<0.01). The level of SOD decreased， while the content of LDH and MDA increased (P<0.01). Compared to the model group， LVEDD and LVESD levels in the low， middle and high dose group of Huangqi Baoxin Decoction were reduced to varying degrees， with increased EF and FS (P<0.05~0.01). They experienced an increased LVDP，±dp/dtmax and decreased LVEDP(P<0.05~0.01)， decreased HWI and LVMI(P<0.05~0.01). And the myocardial cells were neatly arranged， the hyperplasia of fibrous tissue were relived. Besides， the levels of NT-proBNP， ST2， Renin， AngⅡ and ALD went down on diverse degree(P<0.05~0.01)， with decreased LDH and MDA and increased SOD(P<0.05~0.01). There was a dose-effect relationship among the groups of different doses towards the improvement on the above indexes. CONCLUSION Huangqi Baoxin Decoction can improve the cardiac hypertrophy and fibrosis in rats with dilated cardiomyopathy. It also improves the cardiac function and inhibits ventricular remodeling by regulating the oxidation factors and inhibiting the activation of RAAS neuroendocrine factors.

Acetylated Modification of Polysaccharides from Stems and Leaves of Abelmoschus Manihot and Its Immunoregulatory Activity

PAN Xin-xin, JIANG Shu, ZHU Yue, QIAN Da-wei, YAN Hui, DUAN Jin-ao

2017, 33(2): 167-172.

[Abstract](1267) [PDF 931KB](1120)

Abstract:
OBJECTIVE Acetylated modification was applied to modify the structure of polysaccharide from stems and leaves of Abelmoschus manihot， and the in vitro immunoregulatory activity of polysaccharides were evaluated， which was expected to provide the scientific evidence for the resource utilization of A. manihot disused parts. METHODS The crude polysaccharide (SLAMP) was obtained by water extraction and alcohol precipitation， and neutral polysaccharide (SLAMP-a) was further prepared by DEAE-52 ion exchange column. Acetic anhydride was used as acetylating agent to synthesize three acetylated derivatives (SLAMP-a1， SLAMP-a2 and SLAMP-a3). Their structures were preliminarily identified by chemical composition analysis， pre-column derivatization HPLC method and IR spectrum. In addition， in vitro immunoregulatory activity of SLAMP-a and three acetylated derivatives was evaluated by splenocyte proliferation together with its effects on NO production of RAW264.7. RESULTS SLAMP-a showed little immunomodulatory activity and the total sugar content was 99.76%; Among the three derivatives， only SLAMP-a1 could significantly stimulate the proliferation of spleen cells and activate RAW264.7 to product NO. SLAMP-a1 mainly contained glucose with a few of mannose， galactose and arabinose， and the total sugar and DS was 82.50% and 0.62 respectively. CONCLUSION In vitro immunomodulatory activity of polysaccharide SLAMP-a from stems and leaves of A. manihot is significantly enhanced by acetylation and SLAMP-a1 possesses the potential to develop as immunoregulatory agents. The acetylation modification is an effective way for resource utilization of polysaccharide from the disused stems and leaves of A. manihot.

Antibacterial Activities of Grape Seed Procyanidins Against Streptococcus Mutans in Vitro

WANGJing, WANGHui

2017, 33(2): 173-176.

[Abstract](1720) [PDF 404KB](1373)

Abstract:
OBJECTIVE To study the antibacterial activity of grape seed procyanidins (GSPC) against streptococcus mutans in vitro and to explore its anti-caries potential. METHODS Broth micro-dilution method was used to determine minimal inhibitory concentration (MIC)， minimal bactericidal concentration (MBC) and minimal biofilm inhibitory concentration (MBIC); crystal violet semi-quantitative method was used to determine biofilm-formation; anthrone sulfuric acid method was used to test glucan production and pH was used to test acid generation. RESULTS MIC value of GSPC was 12.5 mg/mL while MBC value and MBIC value were both 50 mg/mL. GSPC (≥12.5 mg/mL) could reduce biofilm formation， and especially when its content increased to eight times of MIC content(100 mg/mL)， the inhibition rate was able to achieve 76%. Also it had the ability of inhibiting the glucan production and acid generation at lower content than that of MIC. CONCLUSION GSPC shows antibacterial activity and inhibitory effects on biofilm-formation and cariogenic virulence factors against streptococcus mutans in vitro.

Experiences of Professor HE Juqiao in Treating Idiopathic Oligoasthenoteratozoospermia

ZHUWen-xiong, YANGJing, YUANYi-feng, LIUTao

2017, 33(2): 177-179.

[Abstract](1348) [PDF 370KB](1373)

Abstract:
The clinical experience of professor He Juqiao in recognizing idiopathic oligoasthenoteratozoospermia from the concept of "kidney-tiangui-testicle and epididymis axis"， with the "kidney-tiangui-testicle and epididymis axis" as the direct manifestation of reproductive function governed by kidney being put forward. It is clarified the kidney's physiological effects and pointed out its correlation with visceral regulation， which shares the common concept with hypothalamus-hypophysis-testis axis and gives hints to the clinical thoughts and treatment for idiopathic oligoasthenoteratozoospermia.

Methods for Syndrome Differentiation of Epidemic Hemorrhagic Fever by Professor ZHOU Zhongying Based on Dialectical Thinking

ZHENGZhi-pan, YEFang, ZHUYao

2017, 33(2): 180-181.

[Abstract](1175) [PDF 288KB](1321)

Abstract:
To explore the methods of professor ZHOU Zhongying for syndrome differentiation of epidemic hemorrhagic fever based on dialectical thinking. The following three aspects as simplifing the basic pathogenesis by removing complications， recognizing the convention and being adaptable to changing circumstances by activating the syndrome differentiation， seeking for the truth through proof by contradiction and digging out the theory defect via therapeutic effects exploration are cited to analyze and explore the dialectical thinking of professor ZHOU Zhongying.

Metabolomics Applied to Pediatric Diseases: Opportunities and Challenges

LINLi-li, XIETong, WANGShou-chuan, SHANJin-jun

2017, 33(2): 182-186.

[Abstract](1135) [PDF 429KB](1408)

Abstract:
Childhood is a specific life period. Pediatric disease has its unique physiological and pathological mechanism and thereby it requires distinctive considerations and special treatments. Metabolomics is used to monitor the body's physiological or pathological state by monitoring the changes of endogenous metabolites and biomarkers related to diseases， which provides a new basis for diagnosis and treatment of disease. In recent years， metabolomics has gradually been applied to pediatric diseases， bringing new opportunities for prevention， diagnosis and treatment of the diseases. This paper reviews the application of metabolomics to pathophysiological study of pediatric diseases from the two aspect of "preventive treatment of disease" and "treatment of diseases"， and further put forward the conceive of its application to TCM syndrome research of pediatric diseases.

Research Progress of Cellular Metabolomics

LIWei-wei, YANGYan, WANGShou-chuan, XIETong, SHANJin-jun

2017, 33(2): 187-192.

[Abstract](1383) [PDF 458KB](2218)

Abstract:
Metabolomics aims at qualitative and quantitative analysis of the endogenous metabolites and organism response law of inside and outside factors. Cellular metabolomics is an emerging field of metabolomics in recent years. It's widely used in the study of disease mechanism， pharmacology， toxicology and has obtained a series of achievements. This review outlines the progress of methodology in cellular metabolomics and its application in the field of medicine.

A Metabolomics Study in Pediatric Viral Pneumonia with Syndrome of Wind-Cold

RENJing, WANGShou-chuan, ZHANGQiu-yue, ZHAIWen-sheng, LAIZi-juan, SHANJin-jun

2017, 33(2): 193-197.

[Abstract](1366) [PDF 575KB](1227)

Abstract:
OBJECTIVE Discussion on the application of GC-MS to study the metabolic fingerprinting of serum in pneumonia group with syndrome of wind-cold in order to provide data support for the quantitative study of syndromes in TCM. METHODS In the study of patients with wind-cold(WC) in 30 cases，the normal healthy (NH)in 30 cases，Serum samples were collected and serum metabolites profiles were analyzed by GC-MS coupled with a couple of statistical analyses. RESULTS Clear separations between WC and NH existed. Differential metabolites and several metabolic pathways related organic acids，amino acids，fat were identified. Compared with NH group， the levels of organic acids，amino acids，fat were upregulated，such as L-Alanine， L-Aspartic acid， L-Glutamic acid， Glycine， Serine， L-Threonine， Taurine， L-Lysine，and D-2-Aminobutyric acid， Glycolic acid， Glycerol， 1-Monopalmitin， cholesterol， d-Glucose were down in serum with WC group.And， five abnormal metabolic pathways， such as Alanine metabolism，Glycine metabolism， Taurine metabolism，aa-tRNA biosynthesis and Lysine degradation were identified. CONCLUSION This study detected abnormal metabolites and pathways in WC，which may be due to multiple energy metabolism pathways-virus infected;taking advantage of metablomic techniques，we may establish Characterized Spectrum Database in pediatric viral pneumonia and syndrome differentiation pattern standardization of TCM so as to provide the useful methods in study of syndrome standardization.

Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology

MENGXin, WANGShou-chuan, XIETong, XUJian-ya, SHENCun-si, SHANJin-jun

2017, 33(2): 198-206.

[Abstract](1490) [PDF 1028KB](1458)

Abstract:
OBJECTIVE To detect the relative abundance changes of metabolites in urine and feces of BALB/c mice to seek the biomarkers that contribute to the diagnosis by GC-MS technology. METHODS BALB/c mice were challenged intranasally to establish the RSV pneumonia models. Urine and feces were collected respectively， oximated， derivatized and detected by GC-MS. RESULTS 38 metabolites in urine were detected， among which lactic acid， propanoic acid and L-proline were statistically significant (P<0.05). Moreover， 43 metabolites in feces were detected， and leucine， aspartic acid， cystine， tyrosine and pyrimidine had statistical significance(P<0.05). CONCLUSION GC-MS technology is feasible for seeking biomarkers in urine and feces of mice infected with RSV pneumonia.

Effects of Guben Fangxiao Decoction on the mRNA Expression of Genes Related to Airway Goblet Cell Hyperplasia and Mucus Hypersecretion in a Murine Asthma Remission Model

LU Yuan, ZHAO Xia

2017, 33(2): 207-211.

[Abstract](1199) [PDF 759KB](1038)

Abstract:
OBJECTIVE To investigate the effects of Guben Fangxiao Decoction on mRNA expression of airway goblet cells hyperplasia and mucus hypersecretion-associated genes like IRE1β， SPDEF， AGR2， and mCLCA3 in mice model with asthma at remission stage. METHODS Based on the previous study， OVA sensitization combined with RSV sensitization was applied to establish the asthma model on BALB/c female mice， which were randomly divided into the normal group， model group， Guben Fangxiao Decoction of low， middle and high dose groups， montelukast sodium group and dexamethasone group. qPCR was applied to detect the expression of IRE1β， SPDEF， AGR2 and mCLCA3 mRNA in the lung tissue. RESULTS The expressions of IRE1β， SPDEF and AGR2 mRNA in the lung tissue of the model group were significantly higher than those of the normal group(P<0.05)， while the mCLCA3 mRNA expression was much lower than the normal group(P<0.05). The mCLCA3 mRNA expressions in the lung tissue of Guben Fangxiao Decoction with low， middle and high dosage groups， montelukast sodium group and dexamethasone group experienced decline to different degree. Whereas the mCLCA3 mRNA levels in the Guben Fangxiao Decoction Low dose group and montelukast sodium group were significantly higher than those of the normal group(P<0.05). CONCLUSION Gubenfangxiao Decoction can significantly attenuate RSV-OVA-induced airway goblet cells hyperplasia and mucus hypersecretion in murine asthma remission model. These effects may be mediated， at least partially， by suppressing the mRNA expression of IRE1β， SPDEF and AGR2.

Acting Mechanism of Xiaofeng Xuanqiao Decoction for on Mice Model of Allergic Rhinitis

TAO Jia-lei, WANG Shou-chuan, JIANG Min-cheng, DAI Qi-gang

2017, 33(2): 212-216.

[Abstract](1394) [PDF 544KB](1142)

Abstract:
OBJECTIVE To investigate the therapeutic effects of Xiaofeng Xuanqiao Decoction in treating mice with allergic rhinitis(AR) as well as its mechanism. METHODS 48 BALB/c mice were randomly divided into normal group， model group， Xiaofeng Xuanqiao Decoction group and montelukast group， with 12 mice in each group. OVA sensitized mice were established as the model with gavage for 1 week. Nasal symptom scores， weight， weight of thymus and spleen index， nasal mucosa changes， serum total IgE， ova specific IgE and IL-4， IL-17， IL-33， IFN-γ levels in the thymus of mice were observed. RESULTS Xiaofeng Xuanqiao Decoction could significantly reduce nasal symptoms of AR in mice， reduce the increased weight， thymus weight and thymus index caused by allergic reaction. Besides， it could significantly reduce serum total IgE and ova specific IgE level， and inhibit the production of thymus IL-4， IL-17， IL-33， IFN-γ. In reducing the thymus IL-33 level， Xiaofeng Xuanqiao decoction was better than western medicine montelukast group. CONCLUSION Xiaofeng Xuanqiao Decoction has definite effects for AR in mice. The anti AR effects can be achieved by inhibiting the level of thymic cytokine.

2017 Vol. 33, No. 2

ArchiveMore+

Author field More+

Edit fieldMore+