南京中医药大学学报

Theoretic Study on Diagnosis and Treatment of Knee Osteoarthritis with Pain as Pivot and Truncating and Reversing

WANG Pei-min, XING Run-lin

2016, 32(6): 501-505.

[Abstract](979) [PDF 1064KB](1277)

Abstract:
Knee osteoarthritis(KOA) is a common disease in the elderly. Pain， the most common symptom of KOA， is seen in more than 65% of the patients as main manifestation in clinic. At present， there is no clear pathogenesis and specific treatment of KOA. Conservative treatment， mainly including the control of inflammation and delay of cartilage degeneration， is for controlling pain and other clinical symptoms. KOA belongs to "Bone Bi" and "Bi syndrome" of traditional Chinese medicine. By reviewing the literature on the treatment experience of KOA in orthopedics and traumatology of TCM and combining the experience of department of orthopedics and traumatology in our hospital， diagnostic and therapeutic ideology centered by the control of intractable pain of KOA is put forward with the therapeutic system guided by the concept of truncating and reversing and the combination of cold and heat.

Nine-needle Theory: the Early Academic Kernel of Acupuncture

SUN Zheng, WU Jiu-long, HU Guang-yong, LU Meng-jiang, ZHANG Jian-bin

2016, 32(6): 506-508.

[Abstract](1134) [PDF 1008KB](2297)

Abstract:
By exploring the discussions about the nine-needle in Neijing and making a summary of its number shapes and applications， we find that the nine-needle theory has the feature of concerning morphological structure which is the same as new acupuncture tools and therapies today and very different from "mind-balance" of filiform needle. However， we consider that the nine-needle theory which possesses the feature of "structure-needling" should but did not have enough influence on the development of acupuncture. So we call for paying more academic attention to "physics" during the development of modern acupuncture and making acupuncture more practical.

Discussion on Classification of Infantile Individualize Constitution

LIN Li-li, CHEN Jia-bin, WANG Shou-chuan

2016, 32(6): 509-512.

[Abstract](949) [PDF 1036KB](1234)

Abstract:
Classification of infantile individualize constitution were explored by elaborating the historical evolution， classification and basis of infantile individualize constitution， with comments on infantile constitutions being analyzed， arranged and concluded systematically. Then it is put forward that classification of infantile individualize constitution is associated with six evils externally and yin-yang and five zang organs internally. Clinical care， diagnosis and treatment should be done based on the individual constitution， which provides reference value for the prevention， prognosis and treatment of infantile diseases.

Influences of Toll-like Receptor-4 Gene Polymorphism on Occurance of Stroke Due to Qi Deficiency and Blood Stasis in Han Chinese Men

GU Lian, HUANG Jing-yan, SU Li, LIANG Bao-yun, YANG Jun-wei, WU Yan-li, CHEN Zi-wen, Chen Qing, TANG Nong

2016, 32(6): 513-518.

[Abstract](1037) [PDF 1099KB](1271)

Abstract:
OBJECTIVE To explore the correlation between polymorphisms in the toll-like receptor 4 (rs1927914， rs10759932 and rs11536889) and susceptivity， gene expression， cytokines and blood lipid metabolism in southern Han Chinese with stroke due to wind-phlegm obstruction， qi deficiency and blood stasis. METHODS 559 stroke cases were divided into two groups based on case-control study method， with 248 cases in the qi deficiency and blood stasis group， 311 cases in wind-phlegm obstruction group. In addition， another 605 cases were involved as the healthy control group with the corresponding gender and age. All the subjects were from the Han nationality. Sequenom MassARRAY technology was adopted for testing and genotyping of rs1927914， rs10759932 and rs11536889 in the toll-like receptor 4. Real-time fluorescence quantification(qPCR) was applied for examine mRNA expression. Enzyme linked immunosorbent assay(ELISA) was used to detect the level of interleukin-1β(IL-1β)， interleukin-6 (IL-6)， interleukin-8 (IL-8)， interleukin-12(IL-12) and tumor necrosis factor-α(TNF-α). HITACHI 7600 automatic biochemical analyzer was applied to test the total cholesterol(TC)， triglyceride(TG)， high density lipoprotein cholesterol(HDL) and low density lipoprotein cholesterol(LDL). RESULTS There existed a statistical difference between the male cases of the qi deficiency and blood stasis group and male cases of the control group in the distribution of gene frequency of rs10759932(P<0.05). The locus polymorphism of both rs1927914 (dominant model: OR=0.65， 95%CI=0.43-0.96， Padj=0.031) and rs10759932 (dominant model: OR=0.64， 95%CI=0.41-0.99， Padj=0.046) were closely related with susceptivity of stroke male of qi deficiency and blood stasis type. The IL-8 level of male stroke patients of qi deficiency and blood stasis type with the rs10759932 TT genotype was much lower than those with CC+CT(P<0.01). Rs11536889 polymorphism was notablely related to TG level(additive model: Padj=0.021， dominant model: Padj=0.013) and HDL level(dominant model: Padj=0.032) in stroke patients of qi deficiency and blood stasis type. CONCLUSION TLR4 polymorphisms exert certain influence on the occurance of stroke due to qi deficiency and blood stasis in Han Chinese men and they have been involved in the inflammatory reaction and blood lipid metabolism.

Influences of Method of Soothing Liver， Calming Nerves， Descending Adverse Qi and Protecting Membrane on CGRP and SP in Patients with Non-erosive Reflux Gastroesophageal Disease

DAI Gao-zhong, TIAN Qiu-shi, FAN Xian-jing, ZHU Shi-kai, ZHU Jing-fen, ZHAO Ke-xue

2016, 32(6): 519-523.

[Abstract](1143) [PDF 1376KB](1314)

Abstract:
OBJECTIVE To observe the efficiency of the method of soothing liver， calming nerves， descending adverse qi and protecting membrane in treating patients with non-erosive reflux gastroesophageal disease(NERD) and its role in reducing the expression of CGRP and SP. METHODS 37 patients were randomly divided into treatment group(20 cases) and control group (17 cases). Patients in the control group were treated with omeprazole and domperidone， whereas those in the treatment group were further added with Chinese formula. Questionnaire concerning reflux diseases was used to evaluate the symptoms before and after treatment. Two biopsy samples were taken from the lower esophageal for immunohistochemistry to evaluate the expression of CGRP and SP. RESULTS ①No evident differences were detected in the two groups when it came to the total scores of questionnaire concerning reflux diseases(P>0.05) before treatment. Both the total symptom scores and the symptom scores of burning， chest pain and reflux of gastric contents in the treatment group were lower than those in the control group(P<0.05)， with no evident differences being detected in sour taste/bitter taste in the mouth(P>0.05) after treatment. The total curative rate of the treatment group was 75%， which was higher than 47% of the control group(P<0.05). ②Immunohistochemical test showed that the expression of CGRP and substance P in the treatment group after treatment was lower than that before treatment(P<0.05)， while no significant changes were noticed in the control group(P>0.05). CONCLUSION The method of soothing liver， calming nerves， descending adverse qi and protecting membrane combined with western medicine therapy can efficiently relieve the symptoms and enhance curative efficiency in patients with NERD， whose action mechanism might be related to the inhibition of CGRP and substance P expression， so as to decrease esophageal hypersensitivity， protect the esophageal mucosa and increase its barrier function.

Influence of Ziyin Shugan Xiehuo Formula on Secondary Sex Characteristics and Growth of Girls with Idiopathic Central Precocious Puberty

ZHANG Qing-mei, SHANG Qing, MA Cai-yun, LIN Hai-feng, ZHANG Yu

2016, 32(6): 524-527.

[Abstract](1053) [PDF 1053KB](1305)

Abstract:
OBJECTIVE To study the influence of Ziyin Shugan Xiehuo Formula on secondary sex characteristics and growth of girls with idiopathic central precocious puberty. METHODS 74 cases of idiopathic central type precocious puberty belonging to fire excess from yin deficiency type were selected from June， 2013 to June， 2015 in our hospital and randomly divided into the control group and the observation group， with 37 cases in each group. Patients in the observation group were given Ziyin Shugan Xiehuo Formula for nourishing kidney， soothing liver and purging fire， while those in the observation group was treated with Da Buyin Pill. After 6 months of treatment， TCM symptom improvements and changes of serum LH， FSH， E2， bone age， ovary and uterus volume before and after treatment were observed and compared. The clinical curative effects and adverse reaction of the two groups were evaluated. RESULTS The total effective rate of the observation group was 94.6%， which was significantly higher than 73.0% of the control group(P<0.05). During the treatment， indexes of the two groups including routine urine test， blood urine test， heart， liver and kidney were all within the normal ranges with no obvious adverse being detected. TCM symptom scores of both groups were significantly decreased after treatment(P<0.01)， but the observation group decreased more obviously(P<0.01). Advanced bone ages were notably improved in both groups after treatment(P<0.05~0.01)， with the observation group being more superior to the control group(P<0.01). FSH， LH and E2 levels of two groups after treatment were significantly improved(P<0.05)， but the observation group improved more significantly(P<0.05). Both ovarian and uterine volume of the two groups were significantly reduced after treatment(P<0.05)， but the observation group reduced more obviously(P<0.05). CONCLUSION Ziyin Shugan Xiehuo Formula can obviously decrease sex hormone levels of girls with idiopathic central precocious puberty due to fire excess from yin deficiency， control and improve the occurrence of secondary sex characteristics of the girls， and inhibit the rapid growth of bone age with high safety.

Clinical Efficacy Study on Chaizhi Pinggan Decoction in Treating Functional Dyspepsia of Liver-stomach Disharmony Type and Its Influence on 5-HT and SS

MA Ke-qiang, LI Wan-yu, FENG Xue-zhen

2016, 32(6): 528-531.

[Abstract](1141) [PDF 1053KB](1329)

Abstract:
OBJECTIVE To explore the clinical efficacy of using Chaizhi Pinggan decoction to treat functional dyspepsia of liver-stomach disharmony type and its mechanism. METHODS 110 patients with functional dyspepsia due to liver-stomach disharmony were selected from March， 2015 to March， 2016 in our hospital and randomly divided into the observation group and the control group， with 55 cases in each group. The control group was given domperidone tablets while the observation group were given Chaizhi Pinggan Decoction. After 4 weeks treatment， changes in clinical symptoms and signs， serum serotonin(5HT) and somatostatin(SS) content in the two groups were detected and compared. The recurrence incidence of the two groups during the three-month follow up were observed. RESULTS Such symptoms as abdominal fullness and distention， hypochondriac pain， prone to sign， poor appetite， nausea， belching and vomiting all decreased significantly(P<0.05~0.01)， but the observation group reduced more significantly compared with the control group(P<0.05~0.01). TCM syndrome total scores of both groups decreased significantly after treatment(P<0.01)， but the observation group reduced more significantly(P<0.01). The total efficiency of epigastric pain syndrome and postprandial distress syndrome in the observation group were 92.8% and 92.6%， which were much higher than those in the control group72.4% and 69.2%(P<0.05). After treatment both serum 5HT and SS in the two groups went down significantly(P<0.01)， with more decrease in the observation group(P<0.05). During the three-month follow ups， the recurrence rate of the observation group was 3.6%， lower than 12.7% of the control group(P<0.05). CONCLUSION Chaizhi Pinggan Decoction can significantly improve clinical symptoms of patients with functional dyspepsia due to liver and stomach disharmony， whose mechanism might be related to the increase of 5HT and decrease of SS.

Clinical Research of Du Meridian-dredging Method Combined with Dorsiflexion Ankle Foot Orthotics on Walking Ability in Patients with Hemiplegia

FENG Hui, CHEN Gong, PAN Hua-ping, WU Yun-chuan

2016, 32(6): 532-536.

[Abstract](995) [PDF 1058KB](1164)

Abstract:
OBJECTIVE To observe the influence of Du meridian-dredging method combined with dorsiflexion ankle foot orthotics on walking ability in patients with hemiplegia. METHODS 121 ischemic stroke patients with hemiplegia were divided into the treatment group(n=61) and the control group(n=60) using random single blind control method. Conventional rehabilitation trainings including Bobath technology and ankle-foot orthoses were given to patients in the control group， while those in the treatment group were further given meridian dredging massage. The treatment lasted for 8 weeks in total. Variation of parameters before and after treatment were compared including lower limb movement function score， the fastest walking velocity measured over 10 meters， gait space， time parameters， as well as angle of knee flexion and extension during walk in two groups were compared. RESULTS Before the treatment， all parameters had no significant difference between two groups(P>0.05). After 8 weeks， there was a significant difference on all parameters of both groups(P<0.01)， but the treatment group improved much more than the control group(P<0.05). CONCLUSION The meridian dredging massage with dorsiflexion ankle foot orthotics can improve movement function and walking ability in patients with hemiplegia.

Clinical Observation of Scraping Therapy in Treatment of Allergic Rhinitis of Different Syndromes

AN Hong-li, HUANG Mei

2016, 32(6): 537-539.

[Abstract](5040) [PDF 1071KB](2976)

Abstract:
OBJECTIVE To explore the effect of scraping therapy in the treatment of allergic rhinitis(AR) of different syndromes. METHODS 40 AR patients conforming to the inclusion standard were randomly divided into the control group and treatment group， with 20 cases in each group. Patients in the scraping group received scraping and cupping once a week for 4 weeks in total， while those in the control group were treated with cupping therapy. The symptom changes， clinical efficacy and the content of IgE and IL-4 were examined and compared before and after treatment. RESULTS Significant differences were noticed in the total symptom scores like sneezing， running nose， nasal obstruction and nasal itching before and after treatment(P<0.05). The total effective rate of the control group was 55.0% and that of the treatment group was 89.5%(P<0.05). The content of IgE and IL-4 of two groups were apparently reduced after treatment and the treatment group was superior to the control group(P<0.05). CONCLUSION Scraping therapy achieves definite effect on allergic rhinitis， deserving further study and clinical application.

Discussion on Pathogenesis Essence of Parkinson's Disease Based on Distribution Regularity of TCM Syndrome Elements of 300 Cases

YANG Ning, GUO Wei-feng, LIU Wei-guo, WAN Hai-dong, NING Hou-xu

2016, 32(6): 540-542.

[Abstract](1132) [PDF 1013KB](1329)

Abstract:
OBJECTIVE To discuss the pathogenesis essence of Parkinson's disease through analyzing the distribution regularities of syndrome elements in 300 cases. METHODS Epidemiological cross-sectional survey method was applied to analyze the pathogenesis syndrome elements of 300 cases with Parkinson's disease and summarize its distribution characteristics. RESULTS The total appearance frequency of the eight kinds of syndrome elements was 1180， and the top four pathogenesis syndrome elements are wind， yin deficiency， phlegm and stasis， with the corresponding data being 22.88%， 22.12%， 14.89% and 17.54% respectively. The proportion of patients with the above two or more pathogenesis syndrome elements was 32.33%. CONCLUSION Liver-kidney yin deficiency is considered to be the basis of pathogenesis and wind-phlegm obstruction syndrome and stasis are the center pathogenesis， with the main syndrome type being liver-kidney yin deficiency and wind-phlegm obstruction. Hence， the main therapeutic method for this disease is to nourish liver and kidney， remove phlegm， eliminate wind and dredge collaterals.

Adjustment of Moxibustion on Serum Level of IL-17， RANKL and TGF-β in RA Rats

GAO Zi-shan, GAN Jun-xue, GUO Jiang-yan, FU Shu-ping, GAN Ke

2016, 32(6): 543-547.

[Abstract](1294) [PDF 1062KB](1309)

Abstract:
OBJECTIVE To explore the IL-17 signal transduction mechanism of anti-inflammatory effect of moxibustion on RA rats. METHODS 66 SD rats were randomly assigned to control， model， control+moxa， moxa， IL-17 overexpression+moxa， IL-17 knockdown+moxa， IL-17 overexpression and IL-17 knockdown groups. Complete freund's adjuvant were injected into the right paw of SD rats to establish RA model， and siRNAs were injected for IL-17 overexpression/knockdown models. After 7d， moxibustion treatments were performed for 18d. RESULTS The swelling degree of RA rats and the serum level of IL-17 and RANKL were significantly higher than control group(P<0.01)， and the PWTL of RA rats，serum level of TGF-β were significantly lower than control group. After 18d of moxibustion treatment， the swelling degree of RA rats and the serum level of IL-17，RANKL were significantly lower than control group(P<0.01)，and the PWTL of RA rats and serum level of TGF-β were significantly higher than control group. In addition， the serum level of RANKL in IL-17 knockdown+moxa rats were significantly higher than moxa group(P<0.01); the serum level of RANKL in IL-17 knockdown+moxa rats were significantly lower than moxa group and control group（P<0.01）. CONCLUSION Moxibustion may adjust the serum level of RANKL and TGF-β by supressed the serum level of IL-17 therefore relieving RA in RA rats.

Effect of Fire-needle Intervention on JNK and p38MAPK in Ankle-joint of Rheumatoid Arthritis Rats

SHEN Tian, ZHANG Cai-rong, FU Ronghong, YANG Hongbao, LI Zhong-ren

2016, 32(6): 548-552.

[Abstract](1134) [PDF 1394KB](1467)

Abstract:
OBJECTIVE To observe the influence of fire-needle therapy on JNK and p38MAPK in ankle-joint of rheumatoid arthritis(RA) rats to explore its underlying mechanism in RA treatment. METHODS 40 male SD rats were randomly divided into normal group， model group， fire-needle group and medication(methotrexate， MTX) group， with 10 cases in each group. Fire-needle was applied to Jiaji(EX-B2) and Ashi point every other day， twice altogether. The right hindpaw swelling volume of rats were detected on the 1st， 2nd， 4th， 6th and 8th day， respectively. The pathological changes in synovial tissue of the right ankle joint were observed by optical microscope. The expression of JNK and p38 was detected by immunohistochemical method and Western blot. RESULTS In comparison with the control group， the swelling paw volumes at the 2nd day significantly increased of the rest 3 groups（P<0.01）. Compared to the model group， the swelling paw volumes on the 8th day in fire-needle group decreased significantly（P<0.05）. Serum TNF-α and IL-1β（P<0.05） contents increased in model group comparing with normal group， while decreased in fire-needle group and medication group comparing with model group. The pathological states of synovial tissue of the right ankle joint in both fire-needle and medication groups were relatively lighter than those of the model group. In comparison with normal group， the expression of JNK and p38 in local synovial tissue of the right ankle joint increased significantly in model group（P<0.05～0.01）. Compared with model group， the expression of JNK and p38 of fire-needle and medication group both decreased significantly（P<0.05）. These changes were also found in photographs made by immunohistochemical method. CONCLUSION Fire-needle therapy can dowmregulate the expression of JNK and p38 in local synovial tissue of the right ankle join in MAPK signal transduction pathways， which is the possible mechanism of fire-needle therapy for RA treatment.

2016, 32(6): 553-556.

[Abstract](1041) [PDF 1407KB](1363)

Abstract:

Discussion on the Protection of Aortic Endothelial Function Based on the Rho/ROCK Signaling Pathway of Spontaneous Hypertensive Rats After Grain-sized Moxibustion

ZHANG Hong-ru, GUAN Yuan-yuan, TAO Jia-lei, LU Sheng-feng, NI Guang-xia

2016, 32(6): 557-560.

[Abstract](1201) [PDF 1088KB](1300)

Abstract:
OBJECTIVE To investigate the effect of decreasing blood pressure by moxibustion with seed-sided moxa cone of spontaneously hypertensive rat(SHR) and the protection of vascular endothelial function ，and discuss its possible mechanism. METHODS SHRs were randomly divided into a model group and a moxibustion group. WKY rats were regard as bland group.Rats in model group and bland group did not received moxibustion. Rats in the moxibustion group received moxibustion therapy. Observe the blood pressure，the content of nitric oxide (NO) in serum and aortic morphology change of all groups.Samples of aorta were isolated for Western blotting and the qPCR to identify Rho A，ROCK1 and Rho A，ROCK1 mRNA expression levels. RESULTS Compared with model group， moxibustion group had a decreasing of blood pressure，aortic Rho A，ROCK1 and Rho A ，ROCK1 mRNA expression ，while the NO content in serum increased obviously，the differences were statistically significant（P<0.05）And the aortic intima hyperplasia was not obvious ， just only part of it shed. CONCLUSION Moxibustion with seed-sided moxa cone can reduce the blood pressure of SHR， protect its vascular endothelia function.The mechanism may be related to the inhibition of the expression of Rho/ROCK signal pathway.

Effect of Bushen Huoxue Jiangu Decoction Drug Serum on Osteoblast Proliferation， Differentiation， Maturation and Mineralization in Vitro

CHEN Jupeng, HUANG Guicheng, LI Weidong, LIU Huadong, XING Runlin

2016, 32(6): 561-565.

[Abstract](1147) [PDF 1285KB](1243)

Abstract:
OBJECTIVE To observe the effects of Bushen Huoxue Jiangu Decoction drug serum on osteoblast proliferation， differentiation， maturation and mineralization in vitro， which will provide the theoretical basis for osteoporosis treatment. METHODS Cancellous bone specimens from the femoral head were obtained from patients with femoral neck fracture merger osteoporosis， and the osteoblast cells were seeded and cultured in vitro to establish the osteoblast differentiation model. High， middle and low doses of Bushen Huoxue Jiangu Decoction drug serum were prepared. MTT method， ALP activity assay and alizarin red staining were used to observe the proliferation， activity of ALP and the number of mineral node of cultured osteoblasts in vitro. RESULTS MTT assay displayed different degrees of osteoblast proliferation in high and middle dose groups and showed some dose dependence after 48h of drug intervention. The activities of ALP in high， middle and low dose groups obviously increased after 72h of drug intervention. The deposition of calcium salt crystal in alizarin red staining obviously increased in high dose group after 72h of drug intervention. CONCLUSION Bushen Huoxue Jiangu Decoction drug serum can promote osteoblast proliferation， differentiation， maturation and mineralization in vitro.

The Hypoglycemic Activity of Homogeneous Polysaccharides from Dendrobium Officinale

TANG Zhi-yuan, ZHOU Xiao-yu, FENG Jian, LIU Hua, LI Jun, WU Yi, YE Xu-mei, NI Song-shi

2016, 32(6): 566-570.

[Abstract](1107) [PDF 2282KB](1443)

Abstract:
OBJECTIVE To characterize the homogeneous polysaccharides from Dendrobium officinale Kimura et Migo and study the hypoglycemic activity of the isolated polysaccharides. METHODS DEAE-cellulose anion-exchange chromatography was used to isolate homogeneous polysaccharides from crude polysaccharides (DOP); Monosaccharide composition was analysed after trimethylsilation; and the chemical structure of isolated polysaccharide was characterized with infrared spectroscopy; The effects of isolated polysaccharides on the levels of fasting blood glucose， plasma insulin， and glycosylated serum protein were estimated using diabetic mice models induced by alloxan. RESULTS Four isolated polysaccharides were isolated from DOP， in which DOP1 had the highest content. DOP1 was composed of arabinose， galactose， glucose and mannose in a molar ratio of 3.5∶1.2∶1.5∶1.0. The infrared spectroscopy of various isolated polysaccharides showed that they all had the obvious characteristic absorption peaks of corresponding monosaccharides. The levels of fasting blood glucose and glycosylated serum protein decreased obviously after a 21 day ig administration in diabetic mice models（P<0.01）， in addition， all the four isolated polysaccharides increased glycosylated serum protein levels in varying degrees after a 21 day dosage (P<0.05~0.01). DOP1 displayed the optimal effects on the improvements of above three biochemistry indexes compared with the other three isolated polysaccharides. CONCLUSION Four isolated polysaccharides from DOP displayed hypoglycemic activity in diabetic mice models induced by alloxan.

Network-assisted Investigation into Mechanism of Dachuanxiong Fang on Migraine

YUAN Rong-gao, GU Ming-hua, GU Jiang-yong

2016, 32(6): 571-576.

[Abstract](1003) [PDF 1242KB](1525)

Abstract:
OBJECTIVE To understand the mechanism of Dachuanxiong Fang on migraine. METHODS Data mining， Molecular docking and Network Analysis were employed to investigate the active compounds and key target protein. RESULTS 38 active hits were identified by virtual screening， of which， most compounds had good oral bioavailability and might be optimal CNS exposure. Among them， 16 molecules (senkyunolide M， levistolide A， chuanxiongterpene， riligustilide， etc.) could significantly contribute to alleviate migraine. And they could target 18 protein (such as CGRP， p38， TNF-α， iGluR5， NOS) to inhibit neurogenic inflammation and neutrotransmitters， to reduce the sensitive of nociceptors originated in the trigeminal ganglion， and to combat the genesis and propagation of cortical spreading depression. CONCLUSION It was illustrated the molecular mechanism and the key active compounds of Dachuanxiong Fang， which was helpful for wet experiments to explore targets and active compounds.

Rapidly Determination of Major and Minor Elements in Different Processed Products of Pyrite by X-Ray Fluorescence Spectrometry

ZHANG Meng-qun, CHEN Yi-jun, WU Yu, SHI Ye-dong, LI Wei-dong

2016, 32(6): 577-580.

[Abstract](969) [PDF 1023KB](1170)

Abstract:
OBJECTIVE A comprehensive understanding of the major and minor elements in different processed products of Pyrite， providing scientific basis for the clinical rational use of Pyrite. METHODS The determination method of 10 components including Fe，S，Si，Al and other major and minor components in different processed products of Pyrite by X-ray fluorescence spectrometry was established. RESULTS The detection limit of Fe，S，Mn，Si，Al，Ti，Ca，P，Zn and Pb was 13.37，36.92，7.56， 68， 29， 77.47， 12.46， 22.54， 21.64， 4.55， 3.24mg/kg. Determination of standard material composition of GBW (E) 070088 (iron ore) and GBW 07267 (pyrite) for 5 repetitions.The accuracy were 94%~107% and the RSD were 1.0%~10.1%. CONCLUSION The method can be used to determine the major and minor elements in different processed products of Pyrite， and it is accurate and convenient.

Study on Different Compatibility of Shuangshen Pingfei Decoction Influence on Decocting Rate of Mangiferin in Anemarrhena asphodeloides

JIANG Su, CHEN Ya-jun, ZHENG Yun-feng, LI Cun-yu, FAN Xin-sheng, PENG Guo-ping

2016, 32(6): 581-584.

[Abstract](980) [PDF 1083KB](1307)

Abstract:
OBJECTIVE To explore the influence of different compatibility of Shuangshen Pingfei decoction on the decocting rate of mangiferin in Anemarrhena asphodeloides， and ingredients to research influence on the mechanism of the solution rate. METHODS To disassemble formulas on the basis of the prescription of traditional Chinese medicine containing categories of ingredients. With decocting rate of mangiferin in Anemarrhena asphodeloides Bge as indexes， analyse different compatibility influence on mangiferin decocting rate. Based on these results of disassembled formulas， the representative alkaloid 1-Deoxynojirimycinand amino acid in different compatibility are used to research the influence of compositions of white mulberry on the decocting rate of mangiferin in Anemarrhena asphodeloides Bge and discuss the influence of the decocting rate mechanism with Anion and cation exchange resin processing of samples. RESULTS Research findings shows that the decocting rates of mangiferin in Anemarrhena asphodeloides Bge decoction， prescription combined decoction， Anemarrhena asphodeloides Bge-saponins from Chinese herbal compound， Anemarrhena asphodeloides Bge-phenolic acid from Chinese herbal compound are 97.33%， 47.03%， 94.08%， 57.53%， 34.31%， respectively; Further study shows that Morus alba L. combined with Anemarrhena asphodeloides Bge have the most obvious effect on extraction yield of mangiferin. Anemarrhena asphodeloides Bge decoting with total alkaloids of Morus alba L， DNJ， glycine， β-alanine， respectively. The decocting rate of mangiferin in each group was 16.64%， 28.13%， 40.75% and 48.56%， respectively. CONCLUSION Our results suggest that the decocting rates of mangiferin are mainly influenced by Morus alba L. We also conjectured that alkaloids and amino acids of Morus alba L. form complexes with mangiferin.

Simultaneously Determination of Eight Components in Schisandra chinensis Georgi by UPLC-MS/MS

XIE Tong, XU Jian-ya, SHEN Cun-si, DI Liu-qing, WANG Shou-chuan, SHAN Jin-jun

2016, 32(6): 585-588.

[Abstract](1065) [PDF 1701KB](1349)

Abstract:
OBJECTIVE To develop a sensitive and selective ultra performance liquid chromatography-triple quadrupole mass spectrometer (UPLC-MS/MS) method for quantification of eight components (chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A) in Schisandra chinensis Georgi. METHODS The separation was performed on a Thermo BDS Hypersil C₁₈ (2.1mm×100mm， 2.3μm) column. The mobile consisted of acetonitrile (A) and 0.1% formic acid aqueous with a gradient elution at a flow-rate of 0.25mL/min. The detection was acquired by MRM. The column temperature was 40℃. RESULTS The linear ranges of chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A were 0.975~62.5ng/mL (r=0.9998)， 4.875~2500ng/mL (r=0.9997)， 0.975~62.5ng/mL (r=0.9994)， 0.0975~12.5ng/mL (r=0.9996)， 0.04883~6.25ng/mL (r=0.9999)， 4.875~2500ng/mL (r=0.9998)， 0.4883~250ng/mL (r=0.9999)， 0.4883~250ng/mL(r=0.9998)， respectively. The recoveries were 98.75%，96.44%，101.13%，99.41%，102.26%，98.45%，96.75%，99.42%. CONCLUSION The proposed method enables quantification for the study of the compounds of chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A in Schisandra chinensis Georgi

Exploration on Medication Rule in Prescriptions of Professor Wang Canhui in Treating Chronic Atrophic Gastritis

CHEN Ying-ying, LIU Tao

2016, 32(6): 589-591.

[Abstract](1141) [PDF 1072KB](1524)

Abstract:
OBJECTIVE To explore the prescription rules of professor Wang Canhui in treating chronic atrophic gastritis(CAG) through data mining. METHODS 305 medical records of 124 outpatients with chronic atrophic gastritis treated by professor Wang Canhui with good clinical outcomes were recorded. Both commonly used herds and herb categories were summarized by applying frequency analysis with SPSS 17.0 and also clustering analysis was done to excavate the coreprescriptions and commonly used herb pairs. RESULTS The top three herbs on the frequency list of herb use were deep-fried Atractylodes macrocephala Koidz， Radix Pseudostellariae and Curcuma zedoaria. The commonly used herbs are qi-tonification herbs， heat-clearing herbs， qi-regulation herbs and herbs for invigorating blood circulation and eliminating stasis， with two core prescriptions and four commonly used herb pairs being excavated. CONCLUSION Professor Wang Canhui focuses on protecting the vital qi of spleen and stomach when treating patients with chronic atrophic gastritis and advocates the method of tonifying spleen qi， clearing away depressed heat， regulating qi and blood.

Clinical Experience of Professor Chen Xiaohu in Treating Arrythmia

ZHU Xian-hui

2016, 32(6): 592-594.

[Abstract](1118) [PDF 1009KB](1762)

Abstract:
This paper summarized the clinical experience of Professor Chen Xiaohu in treating arrhythmia with the integrated Chinese-Western therapy based on his double heart concept. It is believed in Chinese medicine that the pathogenesis of arrhythmia is rooted in qi deficiency， yin deficiency and blood deficiency， with the branch being qi stagnation， phlegm， fire and stasis. Feeling ill at ease is considered as the center pathogenesis， with liver， spleen and kidney being involved. Hence， the therapeutic method is to calm the mind and nerves， together with soothing liver， regulating spleen and nourishing kidney， through herb medication and talk therapy. Such formulas as modified Shengmai Powder， Zhi Gancao Decoction， Ergui Pill are applied to treat arrythmia.

Curative Effect Observation of Yinzhihuang Combined with Ursodesoxycholic Acid and Transmetil in Treating Intrahepatic Cholestasis During Gestation Period

WANG Xin-yan, QIU Ming-juan, LU Ting, CHEN Yun-jie

2016, 32(6): 595-597.

[Abstract](1285) [PDF 1021KB](1333)

Abstract:
OBJECTIVE To investigate the clinical effects of combined treatment of Chinese and western medicine including Yinzhihuang， ursodeoxycholic acid and transmetil in the treatment of intrahepatic cholestasis of pregnancy. METHODS 80 cases with intrahepatic cholestasis of pregnancy were divided into observation group and control group， with 40 cases in each group. Patients in the observation group were administrated with traditional Chinese medicine Yinzhihuang and western medicine transmetil and ursodeoxycholic acid， while those in the control group were only given ursodeoxycholic acid and transmetil. Then clinical symptoms， changes of serum total bile acid(TBA)， alanine aminotransferase(ALT)， aspartate aminotransferase (AST)， total bilirubin (TB)， clinical effects and delivery outcomes were compared between the two groups. RESULTS Serum total bile acid， total bilirubin alanine aminotransferase and aspartate aminotransferase in the two groups decreased significantly after treatment and all these indicators in the observation group were significantly lower than those in the control group. The total efficiency of the observation group was much higher than that of the control group. There existed statistical significance in premature delivery， incidence of fetal distress， cesarean section， postpartum hemorrhage when comparison was made between the two groups(P<0.05). CONCLUSION Combining traditional Chinese medicine with western medicine for the treatment of the intrahepatic cholestasis of pregnancy exhibits excellent clinical effects and good perinatal prognosis， which deserves further clinical promotion.

Futong Xiere Formula Through Nasal Feeding for Treating 20 Cases with Hepatic Coma Due to Cirrhosis of Liver and Upper Gastrointestinal Bleeding

YUAN Chao, WU Wen-ping

2016, 32(6): 598-600.

[Abstract](1041) [PDF 1022KB](1229)

Abstract:
OBJECTIVE To observe the clinical effects of Futong Xiere Formula from professor Huang Bao-zhong， a distinguished veteran doctors of TCM， in treating hepatic coma due to cirrhosis of liver and upper gastrointestinal bleeding through nasal feeding. METHODS 40 cases diagnosed with decompensated liver cirrhosis combined with upper gastrointestinal bleeding and hepatic coma in our hospital were collected from August， 2007 to December， 2012 and divided into observation group and treatment group， with 20 cases in each group. Patients in the control group were given conventional treatment including stopping bleeding， resisting hepatic coma， protecting liver and supporting therapy. Based on this， patients in the observation group were further given Futong Xiere Formula through nasal feeding. Then the clinical effects were analyzed between the two groups， together with hospitalization time and costs. RESULTS The total curative rate was 90% in the observation group and 60% in the control group. The observation group was superior to the control group in reducing total bilirubin and blood ammonia level(P<0.05). CONCLUSION Conventional treatment combined with herbal therapy through nasal feeding based on syndrome differentiation can efficiently stop bleeding， improve coma condition and enhance the results.

2016 Vol. 32, No. 6

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